en: carrion biogeochemical-cycling wolf decomposition detritus bacteria soil-biodiversity carcass yellowstone fungi

en:
Site	ID for different carcasses across the Yellowstone Landscape
Location	Sampling location, either under carcass or control plot
Species	Indicating whether carcass was from bison or elk
Gender	Gener of carcass; F = female, M = male, ? = unknown
Lat	Latitude
Long	Longitude
DSK	Days since kill; in days
Soil.N	Soil N concentration (%)
Soil.C	Soil C concentration (%)
Veg.C	Vegetation C concentration (%); dominant grasses sampled
Veg.N	Vegetation N concentration (%); dominant grasses sampled
Veg.CtoN	Vegetation C to N ratio; dominant grasses sampled
Soil.resp	Soil respiration (g m-2 h-1)
soil.temp	Soil temperature (°C)
Soil.moist	Soil moisture (volumentric; %)
CRR	Cotton rotting rate
bacteria.richness	Bacteria richness
bacteria.diversity	Bacteria diverstiy
fungi.richness	Fungi richness
fungi.diversity	Fungi diversity
bacteria.abundance	Bacteria abundance
fungi.abundance	Fungi abundance

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:

Risch, A.C., Frossard, A., Schütz, M., Frey, B., Morris, A.W., Bump, J.K. (accepted) Effects of elk and bison carcasses on soil microbial communities and ecosystem functions in Yellowstone, USA. (accepted). Functional Ecology
doi: ...

Methods
Study area and study sites
This study was conducted in YNP’s Northern Range (NR), located in north-western Wyoming and south-western Montana, USA (~44.9163° N, 110.4169° W). The NR expands over ~1000 km2 and features long cold winters and short dry summers. Grasslands and shrublands dominate the NR that is the home of large migratory herds of bison (winter counts 2017: ~3919 individuals; Geremia, Wallen, & White, 2017) and elk (~5349 individuals) as well as their main predators, approximately five packs of wolves with a total of 33 individuals (Smith et al., 2017). As part of a long-term research program within YNP, wolf predation has been studied since their reintroduction in 1995.
For our study, we received ground-truthed coordinates of bison and elk carcasses from winter 2016/17 (November 2016 through April 2017) from the YNP Wolf Project. Between June 20 and July 1, 2017, we visited 24 carcasses in total. At five sites, we could not sample as the carcasses were no longer found. In total we located remains (hairmats, rumen content, bones, teeth) of 19 adult male and female carcasses (7 bison, 12 elk; Supplementary Table 1). Live body weights of adult bison and elk are approximately 730 kg (male bison), 450 kg (female bison), 330 kg (male elk), and 235 kg (female elk, Meagher, 1973; Quimby & Johnson, 1951).
The kills and subsequent consumption happened between 34 and 173 days prior to our sampling (hereafter “days since kill”, DSK), for which we accounted in our statistics. Note that wolves and other scavengers consumed the soft tissue of the carcasses quickly, hence, there is close to no soft tissue left for decomposition as compared to an intact body left on the soil surface. The 19 carcass sites covered the extent of YNP’s NR, with both bison and elk carcasses showing similar distributions; elevation ranged from 1703 to 2884 m a.s.l. (Supplementary Fig 1 & Supplementary Table 1). The carcasses were all located in grassland or sage-brush shrubland, with or without sparsely scattered trees, and both bison and elk carcasses showed the same distribution of DSK. At each study site, we selected a reference plot (hereafter “control”) that was of comparable size, slope aspect and vegetation to the carcass location (hereafter “carcass”). The control was at least 10 m away (Danell, Berteaux, & Brathen, 2002; Melis et al., 2007) from the carcass itself to ensure the absence of potential direct and indirect carcass effects (paired design; (Bump, Webster, et al., 2009; Bump, Peterson, et al., 2009).

Ecosystem functions and soil properties
We randomly collected 50 g of mineral soil from three locations on both control and carcass plots to a depth of 5 cm with sterile techniques and gently mixed the material to obtain a composite sample. Half the soil sample was immediately bagged in plastic bags (whirl packs), stored in a cooler with ice packs (~5 ºC), sieved (2-mm) and frozen within 4-6 hours of collection to assess soil microbial communities. For this purpose, we extracted total genomic DNA from 0.5 g soil using the PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany). DNA concentrations were measured using PicoGreen (Molecular Probes, Eugene, OR, USA). PCR amplifications of partial bacterial small-subunit ribosomal RNA genes (region V3–V4 of 16S rRNA) and fungal ribosomal internal transcribed spacers (region ITS2) were performed as described previously (Frey et al., 2016). Each sample consisting of 40 ng DNA was amplified in triplicate and pooled before purification with Agencourt AMPure XP beads (Beckman Colter, Berea, CA, USA) and quantified with the Qubit 2.0 fluorometric system (Life Technologies, Paisley, UK). Amplicons were sent to the Genome Quebec Innovation Center (Montreal, Canada) for barcoding using the Fluidigm Access Array technology and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA).
Quality control of bacterial and fungal reads was performed using a customized pipeline (Supplementary Table 2; Frey et al., 2016). Paired-ends reads were matched with USEARCH (Edgar & Flyvbjerg, 2015), substitution errors were corrected using Bayeshammer (Nikolenko, Korobeynikov, & Alekseyev, 2013) and PCR primers were trimmed (allowing for 1 mismatch, read length >300 bp for 16S and >200 bp for ITS primers) using Cutadapt (M. Martin, 2011). Sequences were dereplicated and singleton reads removed prior to clustering into operational taxonomic units (OTUs) at 97% identity using USEARCH (Edgar, 2013). The remaining centroid sequences were tested for the presence of ribosomal signatures using Metaxa2 (Bengtsson-Palme et al., 2015) or ITSx (Bengtsson-Palme et al., 2013). Taxonomic assignments of the OTUs were obtained using Bayesian classifier (Wang, Garrity, Tiedje, & Cole, 2007) with a minimum bootstrap support of 60% implemented in mothur (Schloss et al., 2009) by querying the bacterial and fungal reads against the SILVA Release 128 (Quast et al., 2013) and UNITE 8.0 (Abarenkov et al., 2010) reference databases for 16S and ITS OTUs, respectively.
Abundances of the bacterial 16S rRNA gene and fungal ITS amplicon were determined by quantitative real-time PCR (qPCR) on an ABI7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) as described previously (Frossard et al., 2018). The same primers (without barcodes) and cycling conditions as for the sequencing approach were used for the 16S and ITS qPCR. Three standard curves per target region were obtained using tenfold serial dilutions of plasmids generated from cloned targets (Frey, Niklaus, Kremer, Lüscher, & Zimmermann, 2011). Data were converted to represent mean copy number of targets per gram of soil (dry weight).
The other half of the soil sample was bagged in paper, dried to constant weight at 60°C, passed through a 2 mm sieve and analyzed for total C and N concentration with a CE Instruments NC 2100 soil analyzer (CE Elantech Inc., Lakewood NJ, USA). We also collected 20 mature and undamaged leaves of the dominant grass species growing on control and carcass sites, but taxa were not recorded. The plant material was dried at 60°C, finely ground till homogenized and also analyzed to obtain total C and N concentrations. Soil temperature (10 cm depth) was measured with a waterproof digital thermometer (Barnstead International, Dubuque IA, USA) at three locations each at the control and carcass site. Soil moisture (0 – 10 cm depth) was measured with time domain reflectometry (Field-Scout TDR-100; Spectrum Technologies, Plainfield IL, USA) at five randomly chosen points on control and carcass sites. We measured soil respiration at five randomly chosen points at both control and carcass sites with a PP-Systems SRC-1 soil respiration chamber (closed circuit) attached to a PP-Systems EGM-4 infrared gas analyzer (PP-Systems, Amesbury, MA, USA). For each measurement the soil chamber (15 cm high; 10 cm diameter) was tightly placed on the soil surface, after clipping plants to avoid measuring plant respiration or photosynthesis. Measurements were conducted over 120 s.
In addition, we assessed the decomposition rates of standardized OM using the cotton strip assay (Latter & Howson, 1977; Latter & Walton, 1988). Cotton cloth tensile strength loss (CTSL) is a measure of decomposition, and an index to express the combined effect of soil microclimatic, physical, chemical and biological properties on decomposition while accounting for OM quality (Latter & Walton, 1988; Risch, Jurgensen, & Frank, 2007; Withington & Sanford Jr., 2007). We placed five 20 cm wide x 13 cm long sheets of 100% unbleached cotton cloth (American Type SM 1/18’’, Warp: 34/1, Weft: 20/1, Weave plain, 29.5 picks/cm warp, 22 picks/cm weft, 237 g/m2; Daniel Jenny & Co., Switzerland;) at each carcass and control site vertically into the soil by making slits with a flat spade to a depth of 12 cm. We inserted each cloth with the spade, and then pushed the slit closed to assure tight contact with the soil. The cloths were retrieved after 18 to 27 days. After retrieval, the cloths were air-dried, remaining soil gently removed by hand, and 1.5 cm wide strips were cut at the 3.5-5.0 cm (top) and the 9-10.5 cm (bottom) soil depth. The strips were equilibrated at 50 % relative humidity and 20°C for 48 hours (climate chamber) prior to strength testing (Scanpro Awetron TH-1 tensile strength tester; AB Lorentzen and Wettre, Kista, Sweden). Cotton rotting rate (CRR) = (CTScontrol - CTSfinal/CTSfinal)1/3 * (365/t), where CTScontrol is the cotton tensile strength of a control cloth and CTSfinal the cotton tensile strength of the incubated sample, t is the incubation period in days. Control cloths were inserted into the ground and immediately retrieved to account for tensile strength loss associated with cloth insertion. We averaged the CRR of top and bottom strips for further analyses as no difference was found between the two. All sampling and cloth insertion took place between June 20 and July 1, 2017, cloths were retrieved between July 17 and 20, 2017. Soil respiration, average CRR, vegetation N concentration and vegetation C:N ratio are defined as ecosystem functions, soil C and N concentration, soil temperature and moisture as soil abiotic properties, and bacterial and fungal richness (number of taxa), diversity (Shannon) and abundance as soil biotic properties.

Statistical analyses
Univariate analyses for ecosystem functions, soil biotic and abiotic properties
We tested whether individual ecosystem functions, soil biotic and abiotic properties differed between carcass and control (“Location”), bison and elk (“Species”) and days since kill (“DSK”). For this purpose, we used linear mixed effect models (LMM, “nlme” package v 3.1 – 131.1 in R v 3.4.4; Pinheiro, Bates, DebRoy, & Sarkar, 2018; R Core Team, 2019) with Location, Species, Location x Species and DSK as fixed effects. Site was included as random effect to account for the paired design. We developed a separate model for all dependent variables. All but bacterial richness, fungal richness, fungal diversity and vegetation N concentration were natural-log transformed to meet model assumptions. For each LMM, we calculated contrasts to assess the specific comparisons we were interested in with the “lsmeans” package v 2.27-62 (Lenth & Love, 2018): 1) carcass vs control, 2) carcass bison vs control bison, and 3) carcass elk vs control elk. We also tested whether we had differences between bison and elk carcasses or the sites where bison and elk were killed and included contrasts 4) carcass bison vs carcass elk and 5) control bison vs control elk.
We calculated the log response ratio (LRR = ln[carcass/control]) to obtain carcass effects for all variables for both species separately. LRR < 0 indicates higher value at control compared to carcass, LRR > 0 indicates higher values at carcass compared control. We used LRRs for visualization and to assess spatial patterns in carcass effects across YNP. For this purpose we calculated the Moran’s I statistic for each ecosystem function, soil biotic and abiotic property based on a latitude-longitude matrix with the “moran.test” function in the “spdep” package version 1.1-3 (Bivand et al., 2019).
Multivariate analyses
Rare OTUs, defined as OTUs with a low abundance of reads, were retained in multivariate methods because they only marginally influence these analyses (Gobet, Quince, & Ramette, 2010). Bray–Curtis dissimilarity matrices were generated based on square-root-transformed matrices. We used Principal Coordinate Analyses (PCoA) to assess how soil bacterial and fungal communities differed between control and carcass of bison and elk (“vegan” package v 2.5-4, Oksanen et al., 2019). We then extracted PCoA axes scores 1 and 2 and used LMM (“nlme” package) with Location, Species, Location x Species and DSK as fixed effects. Site was, again, included as random effect. We again calculated the contrasts as described above using the “lsmeans” package. We also assessed how ecosystem functions, and soil abiotic and biotic properties were related to the soil bacteria and fungi community structure associated with bison and elk control and carcasses using the “envfit” function in the “vegan” package (Oksanen et al., 2019).
Indicator species analyses were performed using the multipatt function implemented in the “indicspecies” package version 1.7.6 with 100000 permutations (De Caceres & Jansen, 2016). This step allowed to identify OTUs that led to changes in multivariate patterns between control and carcass of both bison and elk separately (De Cáceres, Legendre, & Moretti, 2010). The multipatt function uses a point biserial correlation coefficient statistical test. Indicator OTUs were defined as bacterial and fungal OTUs with more than 50 sequences, i.e., removing rare taxa and taxa with low abundances containing little indicator information (Rime et al., 2015) and that were significantly correlated with Location (p < 0.05, correlation coefficient > 0.3). A heatmap of these OTUs were generated with the vegan and ggplot2 packages. The indicator analyses were performed in R version 3.3.3 (R Core Team, 2017).

References
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Bivand, R., Altman, M., Anselin, L., Assuncao, R., Berke, O., Blanchet, G., … Yu, D. (2019). spdep: Spatial dependence, weighthing schemes, statistics. R package version 1.1-3.
Bump, J. K., Peterson, R. O., & Vucetich, J. A. (2009). Wolves modulate soil nutrient heterogeneity and foliar nitrogen by configuring the distribution of ungulate carcasses. Ecology, 90(11), 3159–3167.
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Edgar, R. C. (2013). UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods, 10, 996.
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Frey, B., Niklaus, P. A., Kremer, J., Lüscher, P., & Zimmermann, S. (2011). Heavy-machinery traffic impacts methane emissions as well as methanogen abundance and community structure in oxic forest soils. Applied and Environmental Microbiology, 77(17), 6060–6068. doi:10.1128/AEM.05206-11
Frey, B., Rime, T., Phillips, M., Stierli, B., Hajdas, I., Widmer, F., & Hartmann, M. (2016). Microbial diversity in European alpine permafrost and active layers. FEMS Microbial Ecology, 92(3), fiw018.
Frossard, A., Donhauser, J., Mestrot, A., Gygax, S., Bååth, E., & Frey, B. (2018). Long- and short-term effects of mercury pollution on the soil microbiome. Soil Biology and Biochemistry, 120, 191–199. doi:https://doi.org/10.1016/j.soilbio.2018.01.028
Geremia, C., Wallen, R., & White, P. J. (2017). Status report of the Yellowstone bison population, September 2017. Yellowstone National Park, Mammoth, WY, USA: National Park Service, Yellowstone Center for Resources.
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Risch, A. C., Jurgensen, M. F., & Frank, D. A. (2007). Effects of grazing and soil micro-climate on decomposition rates in a spatio-temporally heterogeneous grassland. Plant and Soil, 298(1–2), 191–201. doi:10.1007/s11104-007-9354-x
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Wang, Q., Garrity, G. M., Tiedje, J. M., & Cole, J. R. (2007). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology, 73(16), 5261–5267. doi:10.1128/AEM.00062-07
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Please cite this paper together with the citation for the datafile.

bacteria

display_name: bacteria

biogeochemical-cycling

display_name: biogeochemical-cycling

carcass

display_name: carcass

carrion

display_name: carrion

decomposition

display_name: decomposition

detritus

display_name: detritus

fungi

display_name: fungi

soil-biodiversity

display_name: soil-biodiversity

wolf

display_name: wolf

yellowstone

display_name: yellowstone

en: Elk and bison carcasses in Yellowstone, USA


maintainer: EnviDat Support

en: ground-temperature permos borehole ritigraben permafrost

state: active

type: dataset

en: PERMOS DATA POLICY

media_type: PDF

format: PDF

en: DATA ACCESS

media_type: URL

format: SERVICE

en: Map of PERMOS borehole network

en:
_ENVIDAT NOTE: Data currently unvailable and measures are being taken to recover and restore the data files._

Processed ground temperature measurements at the Ritigraben permafrost borehole (RIT_0102) in canton Valais, Switzerland. The borehole is located at 2690 m asl on a flat site. The surface material is coarse blocks and borehole depth is 30 m. Thermistors used YSI 44006. Year of drilling 2002. This borehole is part of the Swiss Permafrost network, PERMOS (www.permos.ch). Contact phillips@slf.ch for details of processing applied.

borehole

display_name: borehole

ground-temperature

display_name: ground-temperature

permafrost

display_name: permafrost

permos

display_name: permos

ritigraben

display_name: ritigraben

education

fr: Education, science

de: Bildung, Wissenschaft

en: Education and science

it: Formazione e scienza

envidat

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

en: EnviDat: the Environmental Data Portal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: RIT1: Processed permafrost borehole data (2690  m asl), Ritigraben, Switzerland


en: geochemical-solutes stream-water spatially-distributed-sampling stable-water-isotopes precipitation

format: XLS

en: The dataset contains daily measurements of volumes, isotopes and solutes of stream water as well as precipitation measured several locations; meteorological variables are added. Date: 18.07.2019

en:
This dataset contain measurements of solute and stable water isotopes in stream water and precipitation in the Alp catchment and two of its tributaries (between 2015 -2018) . The river Alp is a snow-dominated catchment situated in Central Switzerland characterized by an elevation range from 840 to 1898 m a.s.l. The dataset provides solutes (major anions and cations, trace metals) and stable water isotopes and water fluxes (precipitation rates, discharge) at daily intervals from several sampling locations.
An updated version of the isotope dataset is available here: https://www.doi.org/10.16904/envidat.242

geochemical-solutes

display_name: geochemical-solutes

precipitation

display_name: precipitation

spatially-distributed-sampling

display_name: spatially-distributed-sampling

stable-water-isotopes

display_name: stable-water-isotopes

stream-water

display_name: stream-water

en: Daily data of solute and stable water isotopes in stream water and precipitation in the Alp catchment, Central Switzerland


maintainer: Franziska Koch

en: snow-depth snow-cover gps snow liquid-water-content

en: Dataset with GPS-derived snow cover properties and validation data

media_type: text/csv

en: This file includes the data as described in the Read-me file.

format: CSV

mimetype: text/csv

en: Read-me

media_type: application/pdf

en: The read-me file contains the description of the data provided.

mimetype: application/pdf

en:
This data set includes GPS-derived snow water equivalent (SWE), snow depth (HS) and liquid water content (LWC) data for three entire snow-covered seasons (2015-2016, 2016-2017, 2017-2018) at the study plot Weissfluhjoch 2540 m a.s.l. (Davos, Switzerland). The procedure to derive these snow properties is described in Koch et al. (2019). The novel approach is based on a combination of GPS signal attenuation and time delay.

The dataset also includes corresponding validation data for SWE and HS measured at Weissfluhjoch, and some additional meteorological data used for interpretation of the snow cover evolution.

Please refer to the Read-me file for further details on the data.

These data are the basis of the following publication:
> Koch, F., Henkel, P., Appel, F., Schmid, L., Bach, H., Lamm, M., Prasch, M., Schweizer, J., and Mauser, W., 2019. Retrieval of snow water equivalent, liquid water content and snow height of dry and wet snow by combining GPS signal attenuation and time delay. Water Resources Research, 55(5), 4465-4487. https://doi.org/10.1029/2018WR024431

gps

display_name: gps

liquid-water-content

display_name: liquid-water-content

snow

display_name: snow

snow-cover

display_name: snow-cover

snow-depth

display_name: snow-depth

en: GPS-derived data of SWE, HS and LWC and corresponding validation data


en: photovoltaic snow shortwave-radiation albedo winter-energy-production

en: Data Forward Scattering

media_type: .zip

en: GROUNDEYE model for multiple forward scattering in snow-covered terrain. Meteorological and topographical input data set for the Meierhofertäli near Davos, Switzerland, September 2017 - August 2018. Output data for September 2017 - August 2018 at hourly resolution and every pixel. Post-processing scripts for plotting and analysis.

en:
 Overview
We present GROUNDEYE, a new model of radiative transfer over mountainous terrain, which considers for the first time the forward scattering properties of snow.
Embedded in the surface process model Alpine3D, the new terrain radiation model GROUNDEYE receives interpolated real weather data with diffuse and direct broadband shortwave radiation for each pixel as well as a spatially variable plane albedo from the module SNOWPACK.

 Format
The GROUNDEYE model is written in c++, as is the entire environment of Alpine3d. The input and output data sets are .xlsx or .txt format, pre- and postprocessing including the generation of all figures is in .R format.

 Structure
In Data_Forward_Scattering.zip you will find all necessary data and model details to
reproduce the results of the JGR publication "How forward-scattering snow and terrain change the Alpine radiation balance with application to solar panels"

 - 	Model Input Data contains the meteorological and topographic input data sets, the BRDF, and preprocessing scripts.
 - Model Code contains the full model Alpine3d including the radiative transfer module GROUNDEYE.
 - Model Output Data contains the results of the simulation of terrain irradiance and irradiance of solar panels; hourly resolution, 1. Sptember 2017 - 31. August 2018.
 - Measurements Solar Testsite contains information and measurements of the solar testsite at the Totalp near Davos, Switzerland.
 - Postprocessing contains all R-Scripts used for the analysis and
plotting of the corresponding data.

In each of these folders you will find detailed information in
the file 'About this Folder.txt'.

albedo

display_name: albedo

photovoltaic

display_name: photovoltaic

shortwave-radiation

display_name: shortwave-radiation

winter-energy-production

display_name: winter-energy-production

en: Data, Code Link and Metadata on Forward Scattering of Snow at Totalp


en: phylogenetic-diversity functional-diversity soil grain-size ecological-indicator-values climate taxonomic-diversity

en: Data access

media_type: ZIP

format: ZIP

en:
This dataset comprises a large array of ecological data for the European Alps:

  (1) Current soil and climate predictors at various resolutions.
  (2) GBIF observations of the European Alps Flora (~4,000 species).
  (3) Species habitat suitability maps (1,109 species; based on species observations filtered at 40x40-km)
  at various resolutions used in the study to generate (4); except 'expert'...
  (4) Expert, Taxonomic, phylogenetic and functional diversity of the study region at various resolutions
  (from 100-m to 40-km --> 100-m aggregated & mean to km + non-aggregated/predicted) for CLIM,
  SOIL and CLIM-SOIL models.
  (5) Ecological and altitudinal preferences of the European Alps Flora.
  (6) Data outputs of the related published article.
  (7) All scripts used for analyses.
  (8) Additional files used for analyses.
  (9) Improved set of species habitat suitability maps (~2,600 species; based on species observations
  filtered at 1x1-km) and related taxonomic diversity at 100-m resolution (aggregated to km +
  non-aggregated/predicted) for CLIM, SOIL and CLIM-SOIL models ---> not incorporated in the study.

climate

display_name: climate

ecological-indicator-values

display_name: ecological-indicator-values

functional-diversity

display_name: functional-diversity

grain-size

display_name: grain-size

phylogenetic-diversity

display_name: phylogenetic-diversity

soil

display_name: soil

taxonomic-diversity

display_name: taxonomic-diversity

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Resolution in species distribution models shapes spatial patterns of plant multifaceted diversity


en: water-temperature discharge climate-change-impact hydrology model

en:
This work presents the first extensive study of climate change impacts on rivers temperature in Switzerland. Results show that even for low emissions scenarios, water temperature increase will lead to adverse effect for both ecosystems and socioeconomic sectors (such as nuclear plant cooling) throughout the 21st century. For high emissions scenarios, the effect will be worsen. This study also shows that water warming in summer will be more important in Alpine regions than in lowlands.
This material is distributed under CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/legalcode).

climate-change-impact

display_name: climate-change-impact

discharge

display_name: discharge

hydrology

display_name: hydrology

model

display_name: model

water-temperature

display_name: water-temperature

en: Dataset for: Future water temperature of rivers in Switzerland under climate change investigated with physics-based models


maintainer: Anita Risch

en: ecosystem-functions invertebrates grassland swiss-national-park subalpine exclusion grazing ecosystem-multifunctionality ecosystem-coupling vertebrates

en: Datafile

en:
ID: Plot ID composited of Fence & Treatment number (90 plots total)

Grassland: Name of the six subalpine grasslands where the exclosure setups were erected. In large grasslands (GRIM, MING, STAB), we established four hierarchical exclosure setups (two in short- and two in tall-grass vegetation), whereas in the small grasslands (BOTS, DADA, MARG) only one setup was erected per vegetation type. BOTS = Val dal Botsch, GRIM = Grimmels, MING = Val Mingér, STAB = Alp Stabelchod, DADA = Stabelchod da daint, MARG = Margunet

Fence: Number of exclosure setup (1 - 18). Note that uneven numbers (1,3,5,7,9,11,13,15,17) represent setups established on short-grass vegetation, even numbers (2,4,6,8,10,12,14,16,18) setups established in tall-grass vegetation
Vegetation	Vegetation type, 1 = short-grass vegetation, 2 = tall-grass vegetation; (9 replicate fences on each vegetation type)
Treatment	Progressive animal exclusion, numbers indicate which animals had access to the plot: 1 = Large/medium/small mammals, and invertebrates have access ("L/M/S/I"), 2 = Medium/small mammals, and invertebrates have access (“M/S/I”), 3 = Small mammals and invertebrates have access (“S/I”), 4 = Invertebrates have access (“I”), 5 = No animals have access (“None”)

Bulkden:	Fine fraction soil bulk density (g cm-3)

Soil.moist	: Soil moisture (%)

Soil.org.C: Rhizosphere soil C content (%)

pH: Soil pH

Nema.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil nematode community composition

Nema.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil nematode community composition

Micro.PC1: 	First principal component (PC1) of principal component analysis (PCA) of the soil microbial community composition

Micro.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil microbial community composition

Abv.Inv.PC1: First principal component (PC1) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Abv.Inv.PC2: Second principal component (PC2) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Arthr.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil arthropod community composition

Arthr.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil arthropod community composition

Plant.PC1: First principal component (PC1) of principal component analysis (PCA) of the plant community composition

Plant.PC2: Second principal component (PC2) of principal component analysis (PCA) of the plant community composition

Root.biomass: Root biomass (g m-2)

Micro.biomass	: Microbial biomass carbon (mg kg-1)

Plant.richness: Plant species richness

Plant.N: Plant tissue nitrogen content (%)

Soil.N.min: Soil net nitrogen mineralisation (kg ha-1 season)

Soil.resp: Soil carbon dioxide emissions (soil respiration; g m-2 h-1)

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:
 > Risch AC, Ochoa-Hueso R, van der Putten WH, Bump JK, Busse MD, Frey B, Gwiazdowicz DJ, Page-Dumroese DS, Vandegehuchte ML, Zimmermann S, Schütz M. Size-dependent loss of aboveground animals differentially affects grassland ecosystem coupling and functions. 2018. Nature Communications 9: 3684. [doi: 10.1038/s41467-018-06105-4](https://doi.org/10.1038/s41467-018-06105-4).

Please cite this paper together with the citation for the datafile.

#Methods
Study sites
The experimental exclosure setups were installed within the SNP (IUCN category Ia preserve; Dudley 2008), in south-eastern Switzerland. The park covers 172 km2 of forests and subalpine and alpine grasslands along with scattered rock outcrops and scree slopes. The entire area has been protected from human impact (no hunting, fishing, camping or off-trail hiking) since 1914. Large, fairly homogenous patches of short- and tall-grass vegetation, which originate from different historical management and grazing regimes, cover the park’s subalpine grasslands entirely. Short-grass vegetation developed in areas where cattle used to rest (nutrient input) prior to the park’s foundation (14th century to 1914) (Schütz and others 2003, 2006) and is dominated by lawn grass species such as Festuca rubra L., Briza media L. and Agrostis capillaris L. (Schütz and others 2003, 2006). Today, this vegetation type is intensively grazed by diverse vertebrate and invertebrate communities that inhabit the park and consume up to 60% of the available biomass (Risch and others 2013). Tall-grass vegetation developed where cattle formerly grazed, but did not rest, and is dominated by rather nutrient-poor tussocks of Carex sempervirens Vill. and Nardus stricta L. (Schütz and others 2003, 2006). This vegetation type receives considerably less grazing, with only roughly 20% of the biomass consumed (Risch and others 2013). Consequently, the two vegetation types together represent a long-term trajectory of changes in grazing regimes. Underlying bedrock of all grasslands is dolomite, which renders these grasslands rather poor in nutrients regardless of former and current land-use regimes.
Experimental design
To progressively exclude aboveground vertebrate and invertebrate animals, we established 18 size-selective exclosure setups (nine in short-grass, nine in tall-grass vegetation) distributed over six subalpine grasslands across the SNP (Risch and others 2013, 2015). Elevation differences of exclosure locations did not exceed 350 m (between 1975 and 2300 m a.s.l.). The exclosures were established immediately after snowmelt in spring 2009 and were left in place for five consecutive growing seasons (until end of 2013). They were, however, temporarily dismantled every fall (late October after first snowfall) to protect them from avalanches. They were re-established in the same location every spring immediately after snowmelt. Each size-selective exclosure setup consisted of five plots (2 x 3 m) that progressively excluded aboveground vertebrates and invertebrates from large to small. The plots are labelled according to the guilds that had access to them “L/M/S/I”, “M/S/I”, “S/I”, “I”, “None”; L = large mammals, M = medium mammals, S = small mammals, I = invertebrates, None = no animals had access. As we only had permission to have the experimental setup in place for five consecutive growing seasons, the experiment had to be completely dismantled in the late fall of 2013 and all material removed from the SNP.
Our exclosure design was aimed at excluding mammalian herbivores, but naturally also excluded the few medium and small mammalian predators, as well as the entire aboveground invertebrate food web. A total of 26 large to small mammal species can be found in the SNP, but large apex predators are missing (wolf, bear, lynx) . Reptiles, amphibians and birds are scarce to absent in the subalpine grasslands under study. Only two reptile species occur in the park and they are confined to rocky areas that warm up enough for them to survive. One frog species spawns in an isolated pond far from our grasslands. Only three bird species occasionally feed on the subalpine grasslands. Using game cameras (Moultrie 6MP Game Spy I-60 Infrared Digital Game Camera, Moultrie Feeders, Alabaster, AL, USA), we did observe that the medium- and small-sized mammals (marmot/hares and mice) were not afraid to enter the fences and feed on their designated plots. We never spotted reptiles, amphibians or birds on camera. We distinguished between 59 higher aboveground-dwelling invertebrate taxa that our size-selective exclosures excluded (see also methods for aboveground-dwelling invertebrates below).
The “L/M/S/I” plot (not fenced) was located at least 5 m from the 2.1 m tall and 7 x 9 m large main electrical fence that enclosed the other four plots. The bottom wire of this fence was mounted at 0.5 m height and was not electrified to enable safe access for medium and small mammals, while fencing out the large ones. Within each main fence, we randomly established four 2 x 3 m plots separated by 1-m wide walkways from one another and from the main fence line: 1) the “M/S/I” plots were unfenced, allowing access to all but the large mammals; 2) the “S/I” plots (10 x 10 cm electrical mesh fence) excluded all medium-sized mammals. Note that the bottom 10 cm of this fence remained non-electrified to enable safe access for small mammals; 3) the “I” plots (2 x 2 cm metal mesh fence) excluded all mammals. We double-folded the mesh at the bottom 50 cm to reduce the mesh size to smaller than 1 x 1 cm openings; and 4) the “None” plots were surrounded by a 1 m tall mosquito net (1.5 x 2 mm) to exclude all animals. The top of the plot was covered with a mosquito-meshed wooden frame mounted to the corner posts (roof). We treated these plots a few times with biocompatible insecticide (Clean kill original, Eco Belle GmbH, Waldshut-Tiengen, Germany) to remove insects that might have entered during data collection or that hatched from the soil, but amounts were negligible and did not impact soil moisture conditions within these plots.
To assess whether the design of the “None” exclosure (mesh and roof) affected the response variables within the plots and, therefore, influenced the results, we established an additional six “micro-climate control” exclosures (one in each of the six grasslands) (Risch and others 2013, 2015). These exclosures were built as the “None” exclosures but were open at the bottom (20 cm) of the 3-m side of the fence facing away from the prevailing wind direction to allow invertebrates to enter. A 20-cm high and 3-m long strip of metal mesh was used to block access to small mammals. Thus, this construction allowed a comparable micro-climate to the “None” plots, but also a comparable feeding pressure by invertebrates to the “I” plots. We compared various properties within these exclosures against one another to assess if our construction altered the conditions in the “None” plots. We showed that differences in plant (e.g., vegetation height, aboveground biomass) and soil properties (e.g., soil temperature, moisture) found between the “I” and the “None” treatments were not due to the construction of the “None” exclosure, but a function of animal exclusions, although the amount of UV light reaching the plant canopy was significantly reduced (Risch and others 2013).
Aboveground invertebrate sampling
Aboveground invertebrates were sampled with two different methods to capture both ground- and plant-dwelling organisms: 1) we randomly placed two pitfall traps (67 mm in diameter, covered with a roof) filled with 20% propylene glycol in one 1 x 1 m subplot of the 2 x 3 m treatment plots in spring 2013 (May) and emptied them every two weeks until late September 2013 (Vandegehuchte and others 2017b, 2018). A pitfall trap consisted of a plastic cylinder (13 cm depth, 6.75 cm diameter). Within each cylinder we placed a 100 ml plastic vial with outer diameter 6.70 cm and on top of the cylinder we placed a plastic funnel to guide the invertebrates into the vials. Each trap was cover with a cone-shaped and transparent plastic roof to protect the trap from rain (Vandegehuchte and others 2017b, 2018). Note that in the “None” plots only one trap was placed as control to check for effectiveness of the exclosure. 2) We vacuumed all invertebrates from a 60 x 60 cm area on another 1 x 1 m subplot with a suction sampler (Vortis, Burkhard manufacturing CO, Ltd., Rickmansworth, Hertfordshire, UK) every month from June to September 2013 (Vandegehuchte and others 2017b, 2018). For this purpose, we quickly placed a square plastic frame (60 x 60 x 40 cm) with a closable mosquito mesh sleeve attached to the top edge into the plot from the outside. The suction sample was then inserted into through the sleeve and operated for 45 s to collect the invertebrates (Vandegehuchte and others 2017b, 2018).
We sorted the ≈100 000 individuals collected with both methods by hand and identified each individual morphologically to the lowest taxonomic level feasible (59 taxa, including orders, suborders, subfamilies, families; phylum for Mollusca). These taxa belonged to the following feeding types: 19 herbivores, 16 detritivores, 9 predators, 8 mixed feeders, 5 omnivores and 2 non-classified feeders (or not feeding as adults) (Vandegehuchte and others 2017b). We summed the numbers from the two pitfall traps and the suction sampling over the course of the 2013 season to represent the aboveground invertebrate abundance and community composition of a plot. Note: we did not specifically attempt to catch flying invertebrates with e.g., sticky traps, thus a few flying insects may have been missed with our vacuum sampling approach.
Sampling of plant properties
The vascular plant species composition was assessed at peak biomass every summer (July) by estimating the frequency of occurrence of each species with the pin count method in each plot (Frank and McNaughton 1990). A total of 172 taxa occurred within our 90 plots and we calculated plant species richness for each plot separately. We used the 2013 data in this study. Plant quality was assessed every year in July and September; here we use plant quality at the end of the experiment (September 2013). Two 10 x 100 cm wide strips of vegetation per plot were clipped, combined, dried at 65°C, and ground (Pulverisette 16, Fritsch, Idar-Oberstein, Germany) to pass through a 0.5 mm sieve. Twenty randomly selected samples across all treatments were analysed for N (Leco TruSpec Analyser, Leco, St. Joseph, Michigan, USA) (Vandegehuchte and others 2015). Nitrogen concentrations of the other samples were then estimated from models established for the experiment and the entire SNP relating Fourier transform-near infrared reflectance (FT-NIR) spectra to the measured values of N using a multi-purpose FT-NIR spectrometer (Bruker Optics, Fällanden, Switzerland) (Vandegehuchte and others 2015). Root biomass was sampled every fall by collecting five 2.2 cm diameter x 10 cm deep soil samples (Giddings Machine Company, Windsor, CO, USA) per plot (450 samples year-1). The samples were dried at 30 °C and roots were sorted from the sample by hand. We sorted each sample for 1 h which allowed to retrieve over 90% of all roots present in the samples (Risch and others 2013). The roots were then dried at 65 °C for 48 and weighed to the nearest mg. We averaged the values per plot and used the 2013 data only in this study.
Sampling of edaphic communities
In 2009, 2010, and 2011 we collected three composited soil samples (5 cm diameter x 10 cm depth; AMS Samplers, American Falls, ID, USA) and assessed bacterial community structure using T-RFLP profiling (Liu and others 1997; Blackwood and others 2003; Hodel and others 2014). We detected a total of 89 operational taxonomic units (OTUs). These values are in accordance with other studies reporting OTU richness (Wirthner and others 2011; Zumsteg and others 2012; Meola and others 2014) using T-RFLP profiling, a method that detects the most abundant, and thus likely, the most relevant, taxa. We averaged the data over the three years of collections for our calculations. Microbial biomass carbon (MBC) was determined with the substrate-induced method (Anderson and Domsch 1978) every fall (September) between 2009 and 2013 by collecting three mineral soil samples (5 cm diameter × 10 cm mineral soil core, AMS Samplers, American Falls, ID, USA). The three samples were combined (90 samples for each sampling year), immediately put on ice, taken to the laboratory, passed through a 2-mm sieve and stored at 4°C. Again, we only used the 2013 data in this study.
Soil samples (5 cm diameter x 10 cm depth) to extract soil arthropods were collected in June, July, and August 2011 with a soil corer lined with a plastic sleeve to ensure an undisturbed sample (total of 270 samples). The plastic line core was immediately sealed on both ends using cling film and put into a cooler. All plots were sampled within three days and the extraction of arthropods started the evening of the sampling day using a high-gradient Tullgren funnel apparatus (Crossley and Blair 1991; Vandegehuchte and others 2015). Samples were kept in the extractor for four days and the soil arthropods were collected in 95% ethanol. All individuals were counted and each individual was identified morphologically to the lowest level feasible [76 taxa, including orders, suborders, subfamilies, families (Protura, Thysanoptera, Aphidina, Psylina, Coleoptera, Brachycera, Nematocera, Auchenorryncha, Heteroptera, Formicidae); sub-phylum for Myriapoda, for Acari and Collembola also including morpho-species). Note that we also included larval stages (nine of the 76 taxa) (Vandegehuchte and others 2015). All data were summed over the season. A detailed species list for mites and collembolans is published (Vandegehuchte and others 2017a) [https://doi.org/10.1371/journal.pone.0118679.s001]. Earthworms are rare in the SNP and therefore were not included. We collected eight random 2.2 cm diameter x 10 cm deep soil cores from each plot in September 2013 to determine the soil nematode community composition. The samples were mixed and the nematodes were extracted from 100 ml of fresh soil using Oostenbrink elutriators (Oostenbrink 1960). All nematodes in a 1 ml of the 10 ml extract were counted, a minimum of 150 individuals sample-1 were identified to genus or family level using (Bongers 1988), the numbers of all nematodes were extrapolated to the entire sample and expressed for a 100 g dry sample. In total we identified 63 genus or family levels (Vandegehuchte and others 2015). The list of all the nematodes found is published (Vandegehuchte and others 2015) [http://www.oikosjournal.org/appendix/oik-03341] or DOI: [doi: 10.1111/oik.03341].
We are aware that sampling soil microbes from 2009 to 2011 and soil arthropods in 2011 was not ideal, but we are positive that this does not bias the results. Most of the parameters measured in our experiment either already showed a treatment response after the first growing season (e.g., plant biomass) or did not respond over the entire time experiment (e.g., microbial biomass C). The microbial community composition (2009 – 2011) was highly influenced by inter-annual differences in temperature and precipitation, but did not differ between treatments or vegetation types (Hodel and others 2014). We therefore felt comfortable using the 2009 through 2011 data for describing the soil microbial community in our experimental treatments. Similarly, we are positive that our soil arthropod data are representative. We did assess soil arthropods in August 2012 and found no differences to the August 2011 data. However, we did not feel comfortable combining the 2011 June, July, August data with only August data for 2012 for our analyses.
Sampling of soil properties
We collected three soil samples (5 cm diameter x 10 cm depth) in each plot in September 2013 after removing the vegetation. First, we collected the top layer of mineral soil rich in organic matter, the surface organic layer or rhizosphere, typically 1 to 3 cm in depth with a soil corer (AMS Samples, American Falls, Idaho, USA). Second, we collected a 10 cm mineral soil core beneath this surface layer. The cores for each layer were composited, dried at 65 °C for 48 h and fine-ground to pass a 0.5 mm screen. We then analysed all samples for total C using a Leco TruSpec Analyser (Leco, St. Joseph, Michigan, USA). Mineral soil pH was measured potentiometrically in 1:2 soil:CaCl2 solution with an equilibration time of 30 min.
Soil net N mineralisation was assessed during the 2013 growing season (Risch and others 2015). For this purpose, we randomly collected a 5 cm diameter x10 cm deep soil sample with a soil corer (AMS Samples, American Falls, Idaho, USA) after clipping the vegetation in June 2013. After weighing and sieving (4 mm mesh) the soil, we extracted a 20 g subsample in 1 mol l-1 KCl for 1.5 h on an end-over-end shaker and thereafter filtered it through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnenmühle FineArt GmbH, Dassel, Germany). From these filtrates NO3- concentrations were measured colorimetrically (Norman and Stucki 1981) and NH4+with flow injection analysis (FIAS 300, Perkin Elmer, Waltham Massachusetts, USA) (Risch and others 2015). We dried the rest of the sample 105 °C to constant mass to determine fine,fraction bulk density. A second soil sample was collected within each plot in June 2013 with a corer lined with a 5 x 13 cm aluminium cylinder. The corer was driven 11.5 cm deep into the soil so that the top 1.5 cm of the cylinder remained empty. Into this space we placed a polyester bag (250 µm) filled an ion-exchanger resin to capture the incoming N. The bag was filled with a 1:1 mixture of acidic and alkaline exchanger resin (ion-exchanger I KA/ion exchanger IIIAA, Merck AG, Darmstadt, Germany). We then removed 1.5 cm soil at the bottom of the cylinder and placed a second resin exchanger bag into this space to capture the N leached from the soil column. To assure that the exchange resin was saturated with H+ and Cl- prior to filling the bags, the mixture was stirred with 1.2 ml l-1 HCl for 1 h and then rinsed with demineralized water until the electrical conductivity of the water reached 5 µm cm-1. The cylinder with the resin bags in place was reinserted into the soil with the top flush to the soil surface and incubated for three months. We recollected the cylinders in September 2013. Each resin bag and 20 g of sieved soil (4 mm mesh) from each cylinder were then separately extracted with KCl and NO3- and NH4+ concentrations were measured. Nitrate and NH4+ concentrations of all samples were then converted to a content basis by multiplying their values with fine fraction bulk density. Net N mineralisation was thereafter calculated as the difference between the N content of the samples collected at the end of the three-month incubation (including the N extracted from the bottom resin bag) and the N content at the beginning of the incubation (Risch and others 2015).
Soil CO2 emissions were measured every two weeks between 0900 and 1700 hrs from early May through late September 2013 with a PP-Systems SRC-1 soil respiration chamber (15 cm high, 10 cm diameter; closed circuit) attached to a PP-Systems EGM-4 infrared gas analyser (PP-Systems, Amesbury, MA, USA) on two locations per plot (Risch and others 2013). The chamber was placed on randomly placed, permanently installed PVC collars (10 cm diameter) driven 5 cm into the soil at the beginning of the study (Risch and others 2013). Freshly germinated plants growing within the collars were removed prior to each measurement to avoid measuring plant respiration or photosynthesis. The two measurements collected per plot and sampling date were averaged.
Soil moisture (with time domain reflectometry; Field-Scout TDR-100, Spectrum Technologies, Plainfield, Illionois, USA) and temperature (with a waterproof digital pocket thermometer; Barnstead International, Dubuque, Iowa, USA) were measured at five random locations per plot every two weeks during the growing seasons during the experiment for the 0 to 10 cm depth (Risch and others 2013, 2015). As soil moisture and soil temperature were highly negatively correlated (Risch and others 2013), we only used soil moisture for this study. We used plot-level averages of all values available to capture soil moisture variability during the five years of the experiment. The results remained unchanged when we only used soil moisture from the 2013 growing season.
Numeral calculations and statistical analyses
Ecosystem coupling. We conducted principal component analyses (PCAs; unscaled) at the complete dataset level using the abundances of each taxonomical entity to describe each of the five different communities used in this study: aboveground-dwelling invertebrates, vascular plants, soil microorganisms, soil arthropods and soil nematodes. We retained the first two components (PCA axis 1 and PCA axis 2) of each analysis as we found them to adequately represent the temporal and spatial variability of our 90 treatment plots in previous studies55,67. Together they explained a total of 71.70% of the variation for aboveground invertebrates, 44.36% for plants, 44.85% for soil microorganisms, 61.85% for soil arthropods and 77.19% for soil nematodes. In addition, we used soil pH and soil organic C content as a proxy for soil chemical properties, soil bulk density as a proxy for soil physical properties and soil moisture (negatively correlated with soil temperature) as a proxy for soil micro-climatic conditions for an overall total of fourteen constituents.
We calculated ecosystem coupling9 for each exclosure treatment within each vegetation type (i.e., 2  5 treatment combinations in total) as an integrated measure of pairwise ecological interactions between ecosystem constituents representing ecological communities and the soil abiotic environment. These ecological interactions are defined by non-parametric Spearman rank correlation analyses between two constituents, excluding interactions involving two abiotic constituents (e.g., soil pH vs. soil moisture) and interactions between the first (PC1) and second (PC2) component of each community type, as these are orthogonal by definition. Interactions between abiotic constituents were excluded from the analyses because the focus of our study was on communities and how they interact with one another and their surrounding environment; therefore, including abiotic-abiotic interactions was not of interest here. Given that the effectiveness of our experimental design resulted in that no community composition data of aboveground-dwelling invertebrates was available for the “None” plots (all animals excluded), only thirteen instead of fourteen constituents were included in the ecosystem coupling calculations for this treatment. The complete absence of aboveground invertebrates represents the most extreme case of disturbance between aboveground animal communities and the rest of the ecosystem constituents. This may have resulted in a slight overestimation of ecosystem coupling for these plots.
	Average ecosystem coupling was calculated as follows:
Ecosystem coupling=
where Xi is the absolute Coupling was calculated value of the Spearman’s rho coefficient of the ith correlation for each treatment within each vegetation type (i.e., based on nine replicates each), considering and n is the number of pairwise comparisons considered (n = a total of 80; interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).	Coupling was calculated for each treatment within each vegetation type (i.e., based on nine replicates each), considering a total of 80 interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).
To establish whether constituents were significantly and positively coupled within treatments (i.e., the average of their correlation coefficients were greater than in a null model where correlation only happens by chance), we calculated one-tailed p-values based on permutation tests with 999 permutations.

We considered six ecosystem functions and process rates commonly used to assess ecosystem functioning (Meyer and others 2015; Manning and others 2018). Plant N content represents a measure of forage quality, while plant richness has been shown to stabilise biomass production, thus allowing the system to respond to changes in herbivory. Soil net N mineralisation, soil respiration, root biomass, and microbial biomass represent fluxes or stocks of energy. For all functions and processes higher values represent higher functioning (Manning and others 2018). All these variables were measured in the last year of the experiment (2013). We then quantified ecosystem multifunctionality using the multiple threshold approach (Byrnes and others 2014; Manning and others 2018), which considers the number of functions that are above a certain threshold, over a series of threshold values (typically 10-99%) that are defined based on the maximum value of each function. We weighted all our functions equally for these calculations (Manning and others 2018). The number of functions in a plot with values higher than a given threshold value for the respective function is summed up. The sum represents ecosystem multifunctionality for that plot. Given that choosing any particular threshold as a measure of ecosystem multifunctionality is arbitrary, we calculated the average of thresholds from 10-90% (in 10% intervals) as a more integrated representation of ecosystem multifunctionality.
We used Pearson correlations to explore the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and ecosystem multifunctionality by calculating the slopes of all relationships between ecosystem coupling and multifunctionality for all thresholds between 10 and 99%. We also related ecosystem coupling with the average of multifunctionality at thresholds between 30-80% as explained before and considered this correlation as a robust indication of the type of association between these two variables. In addition, we explored the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and individual ecosystem functions. The effects of exclosures and vegetation type on individual functions and multifunctionality were evaluated using linear mixed effects models ('lme' function of the nlme package), with exclosure and vegetation type as fixed effects and fence as a random factor. All statistical analyses and numerical calculations were done in R version 3.4.0 (R Core Team 2016).

#References

- Anderson J, Domsch K. 1978. A physiological method for the quantitative measurement of microbial biomass in soil. Soil Biol Biochem 10:215–21.
- Blackwood CB, Marsh T, Kim S-H, Paul EA. 2003. Terminal Restriction Fragment Length Polymorphism Data Analysis for Quantitative Comparison of Microbial Communities. Appl Environ Microbiol 69:926–32. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143601/
- Bongers T. 1988. De nematoden von Nederland. Schoorl, The Netherlands: Pirola
- Byrnes JEK, Gamfeldt L, Isbell F, Lefcheck JS, Griffin JN, Hector A, Cardinale BJ, Hooper DU, Dee LE, Duffy JE. 2014. Investigating the relationship between biodiversity and ecosystem multifunctionality: Challenges and solutions. Methods Ecol Evol 5:111–24.
- Crossley DAJ, Blair JM. 1991. A high-efficiency low-technology Tulgren-type extractor for soil microarthopods. Agric Ecosyst Environ 34:187–92.
- Dudley N. 2008. Guidelines for applying protected area managment categories. Gland: IUCN
- Frank DA, McNaughton SJ. 1990. Aboveground biomass estimation with the canopy intercept method: A plant growth form caveat. Oikos 57:57–60.
- Haynes AG, Schütz M, Buchmann N, Page-Dumroese DS, Busse MD, Risch AC. 2014. Linkages between grazing history and herbivore exclusion on decomposition rates in mineral soils of subalpine grasslands. Plant Soil 374.
- Hodel M, Schütz M, Vandegehuchte ML, Frey B, Albrecht M, Busse MD, Risch AC. 2014. Does the aboveground herbivore assemblage influence soil bacterial community composition and richness in subalpine grasslands? Microb Ecol 68:584–95.
- Liu WT, Marsh TL, Cheng H, Forney LJ. 1997. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ Microbiol 63:4516–22. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC168770/
- Manning P, van der Plas F, Soliveres S, Allan E, Maestre FT, Mace G, Whittingham MJ, Fischer M. 2018. Redefining ecosystem multifunctionality. Nat Ecol Evol 2:427–36. https://doi.org/10.1038/s41559-017-0461-7
- Meola M, Lazzaro A, Zeyer J. 2014. Diversity, resistance and resilience of the bacterial communities at two alpine glacier forefields after a reciprocal soil transplantation. Environ Microbiol 16:1918–34. https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.12435
- Meyer ST, Koch C, Weisser WW. 2015. Towards a standardized Rapid Ecosystem Function Assessment (REFA). Trends Ecol Evol 30:390–7. http://www.sciencedirect.com/science/article/pii/S0169534715000968
- Norman R., Stucki JW. 1981. The determination of nitrate and nitrite in soil extracts by ultraviolet spectrophotometry. Soil Sci Soc Am J 45:347–53.
- Ochoa-Hueso R. 2016. Non-linear disruption of ecological interactions in response to nitrogen deposition. Ecology 87:2802–2814.
- Oostenbrink M. 1960. Estimating nematode populations by some selected methods. In: Sasser NJ, Jenkins WR, editors. Nematology. Chapel Hill, NC, USA: University of North Carolina Press. pp 85–101.
- R Core Team. 2016. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing
- Risch AC, Haynes AG, Busse MD, Filli F, Schütz M. 2013. The response of soil CO2 fluxes to progressively excluding vertebrate and invertebrate herbivores depends on ecosystem type. Ecosystems 16:1192–202.
- Risch AC, Schütz M, Vandegehuchte ML, Van Der Putten WH, Duyts H, Raschein U, Gwiazdowicz DJ, Busse MD, Page-Dumroese DS, Zimmermann S. 2015. Aboveground vertebrate and invertebrate herbivore impact on net N mineralization in subalpine grasslands. Ecology 96:3312–22.
- Schütz M, Risch AC, Achermann G, Thiel-Egenter C, Page-Dumroese DS, Jurgensen MF, Edwards PJ. 2006. Phosphorus translocation by red deer on a subalpine grassland in the Central European Alps. Ecosystems 9:624–633.
- Schütz M, Risch AC, Leuzinger E, Krüsi BO, Achermann G. 2003. Impact of herbivory by red deer (Cervus elaphus L.) on patterns and processes in subalpine grasslands in the Swiss National Park. For Ecol Manage 181:177–88.
- Vandegehuchte ML, van der Putten WH, Duyts H, Schütz M, Risch AC. 2017a. Aboveground mammal and invertebrate exclusions cause consistent changes in soil food webs of two subalpine grassland types, but mechanisms are system-speciﬁc. Oikos 126:212–23.
- Vandegehuchte ML, Raschein U, Schütz M, Gwiazdowicz DJ, Risch AC. 2015. Indirect short- and long-term effects of aboveground invertebrate and vertebrate herbivores on soil microarthropod communities. PLoS One 10:e0118679.
- Vandegehuchte ML, Schütz M, de Schaetzen F, Risch AC. 2017b. Mammal-induced trophic cascades in invertebrate food webs are modulated by grazing intensity in subalpine grassland. J Anim Ecol 86:1434–46.
- Vandegehuchte ML, Trivellone V, Schütz M, Firn J, de Schaetzen F, Risch AC. 2018. Mammalian herbivores affect leafhoppers associated with specific plant functional types at different timescales. Funct Ecol 32:545–55.
- Wirthner S, Frey B, Busse MD, Schütz M, Risch AC. 2011. Effects of wild boar (Sus scrofa L.) rooting on the bacterial community structure in mixed-hardwood forest soils in Switzerland. Eur J Soil Biol 47:296–302. http://dx.doi.org/10.1016/j.ejsobi.2011.07.003
- Zumsteg A, Luster J, Göransson H, Smittenberg RH, Brunner I, Bernasconi SM, Zeyer J, Frey B. 2012. Bacterial, Archaeal and Fungal Succession in the Forefield of a Receding Glacier. Microb Ecol 63:552–64. https://doi.org/10.1007/s00248-011-9991-8

ecosystem-coupling

display_name: ecosystem-coupling

ecosystem-functions

display_name: ecosystem-functions

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

exclusion

display_name: exclusion

grassland

display_name: grassland

grazing

display_name: grazing

invertebrates

display_name: invertebrates

subalpine

display_name: subalpine

swiss-national-park

display_name: swiss-national-park

vertebrates

display_name: vertebrates

en: Ecosystem coupling and multifunctionality - exclosure experiment


maintainer: Athanasios Nenes

en: weissfluhjoch hygroscopicity supersaturation dmt-ccn-chamber cloud-modeling ccn sigma-w raclets cloud-droplets

en: Predicted cloud droplet number concentrations Weissfluhjoch

en:
In-situ measurements of aerosol size distributions along with updraft velocities collected from a wind Doppler Lidar are coupled with a state-of-the art droplet activation parameterization (Morales and Nenes, 2014) to determine the cloud droplet number and maximum in-cloud supersaturation that can potentially form over the Weissfluhjoch station between February 24 and March 8 2019. For this, a hygroscopicity parameter of 0.25 is assumed, according to the CCN-derived values. The data file contains:

* Date and time (DD/MM/YYYY hh:mm:ss) in UTC
* The wind-lidar derived standard deviation of updraft velocities (sigma-w) in m/s, used to apply the parameterization employing a single characteristic velocity (Morales and Nenes, 2010)
* The maximum predicted cloud parcel supersaturation (Smax%)
* The total in-situ aerosol number concentration (Naer) in cmˉ³, measured by the SMPS
* The predicted cloud droplet numbers (Nd) in cmˉ³
* The activation fraction (%)

en: CCN data Weissfluhjoch

en:
In-situ CCN measurements were conducted between February 24 and March 8 2019 at Weissfluhjoch. The data file contains:

* Date and time (DD/MM/YYYY hh:mm:ss) in UTC
* Filtered CCN number concentrations (in cmˉ³) measured at 6 discrete supersaturations (SS) ranging from 0.09% to 0.74%. The name of each CCN column is indicative of the SS developed within the instrument.
* Instrument characteristics such as sample temperature (in °C) and pressure (in mbar)
* A code that refers to the reason of filtering.

Missing or filtered CCN values are indicated by "-999", while a declaration of each "Error_code" can be found in the following list:

_Error_code_:

0000: Measurements without error

1000: Period of transient operation after a supersaturation change

2000: Sharp change (decrease) in the room temperature housing the instrument, which induces a reset in column temperature

3000: Irregular values in the room temperature housing the instrument

9999: Missing data

en: Hygroscopicity parameter Weissfluhjoch

en:
The aerosol hygroscopicity parameter is derived combining the CCN and SMPS size distribution data collected between February 24 and March 8 2019 at Weissfluhjoch. The data file contains:

* Date and time (DD/MM/YYYY hh:mm:ss) in UTC
* The instrument supersaturation (SS%)
* The calculated hygroscopicity parameter (kappa)
* The calculated critical dry diameter of the particles (Dcr) in nm.

Missing values are indicated by "-999".

en:
Cloud Condensation Nuclei (CCN) data: A Droplet Measurement Technologies (DMT) single-column continuous-flow streamwise thermal gradient chamber (CFSTGC; Roberts and Nenes, 2005) was deployed at the measurement site Weissfluhjoch (2700 m a.s.l., LON: 9.806475, LAT: 46.832964) to record the in-situ CCN number concentrations between February 24 and March 8 2019 for different supersaturations (SS). To account for the difference between the ambient (~735 mbar) and the calibration pressure (~800 mbar), the SS reported by the instrument is adjusted by a factor of 0.92. The CFSTGC was cycled between 6 discrete SS values ranging from 0.09% to 0.74%, producing a full CCN spectrum every hour. The raw CCN measurements are filtered to discount periods of transient operation and whenever the room temperature housing the instrument changed sufficiently to induce a reset in column temperature. Additional information can be found in Section 2.1.2 [here](https://acp.copernicus.org/preprints/acp-2020-1036/).

Hygroscopicity data: The CCN number concentration measurements were directly related to the size distribution and total aerosol concentration data measured by the Scanning Mobility Particle Size Spectrometer (SMPS) instrument at the same station (https://www.envidat.ch/dataset/aerosol-data-weissfluhjoch) to infer the particles hygroscopicity parameter (kappa). For each SMPS scan, the particles critical dry diameter (Dcr) is estimated by integrating backward the SMPS size distribution, until the aerosol number matches the CCN concentration observed for the same time period as the SMPS scan. Assuming the particle chemical composition is internally mixed, the kappa is determined from Dcr and SS, applying Köhler theory. Additional information can be found in Section 2.2 [here](https://acp.copernicus.org/preprints/acp-2020-1036/).

Predicted cloud droplet numbers: Droplet calculations are carried out with the physically based aerosol activation parameterization of Morales and Nenes (2014), employing the “characteristic velocity” approach of Morales and Nenes (2010). Aerosol size distribution observations required to predict the cloud droplet numbers and maximum in-cloud supersaturation are obtained from the SMPS instrument deployed at Weissfluhjoch. The required vertical velocity measurements are derived from the wind Doppler Lidar (https://www.envidat.ch/dataset/lidar-wind-profiler-data) deployed at Davos Wolfgang and are extracted for the altitude of interest, being 1100 m above ground level for Weissfluhjoch. Additional information can be found in Section 2.3 [here](https://acp.copernicus.org/preprints/acp-2020-1036/).

ccn

display_name: ccn

cloud-droplets

display_name: cloud-droplets

cloud-modeling

display_name: cloud-modeling

dmt-ccn-chamber

display_name: dmt-ccn-chamber

hygroscopicity

display_name: hygroscopicity

raclets

display_name: raclets

sigma-w

display_name: sigma-w

supersaturation

display_name: supersaturation

weissfluhjoch

display_name: weissfluhjoch

en: CCN, hygroscopicity, predicted cloud droplet numbers Weissfluhjoch


maintainer: Matthias Haeni

en: precipitation wind-speed temperature relative-humidity meteorology meteo swiss-forest-lab climate-change long-term par

en: Site photo of the forest station (CIB)

media_type: image/jpeg

en: Site photo of the forest station from below (viewing upwards the slope).

mimetype: image/jpeg

en: Site photo of the field station (CIF)

en: Site photo of the field station from the side.

en: Data plot of the forest station (CIB)

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

en: Site aerial photo (Chironico)

en: Site aerial photo from both Chironico stations. Source: Google. 2016. Site aerial photo from both Chironico stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Forest station data (CIB)

en: Data file.

en: Data plot of the field station (CIF)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Chironico in Switzerland where one station is located within a natural coniferous forest stand (CIB) with Norway spruce (_Picea abies_; 160-180 yrs) and European silver fir (_Abies alba_; 140-160 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, CIF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Chironico is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Chironico, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 2000 onwards


en: precipitation wind-speed temperature relative-humidity swiss-forest-lab meteo meteorology climate-change long-term par

en: Site aerial photo (Bettlachstock)

en: Site aerial photo from both Bettlachstock stations. Source: Google. 2016. Site aerial photo from both Bettlachstock stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Site photo of the forest station (BTB)

en: Site photo of the forest station from the side.

en: Data plot of the forest station (BTB)

en: Field station data (BTF)

en: Site photo of the field station (BTF)

en: Data plot of the field station (BTF)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Bettlachstock in Switzerland where one station is located within a natural mixed forest stand (BTB) with European beech (_Fagus sylvatica_; 170-190 yrs), European silver fir (_Abies alba_; 190 yrs) and Norway spruce (_Picea abies_; 200 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, BTF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Bettlachstock is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

en: Bettlachstock, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


disdrometer-data-laret

maintainer: Daniel Wolfensberger

title_for_slug: disdrometer-data-laret

en: visibility precipitation raclets hydrometeors disdrometer

en: Disdrometer Data Laret

media_type: application/zip

en: The zip folder contains one netCDF file per day for Laret from February 7 to March 29 2019 with a temporal resolution of 30 seconds (time in UTC). The naming convention for the files is Laret_YYYYMMDD.nc and the files include the following: characteristics of hydrometeors (e.g. diameter, fall velocity, amount per diameter class,...), precipitation rate, radar reflectivity, visibility range, weather codes and instrument performance.

mimetype: application/zip

en:
A laser optical disdrometer (Parsivel² , OTT Hydromet) was used to measure hydrometeors by extinction when passing a laser beam. The instrument can classify eight different kinds of precipitation, including rain, hail, snow, drizzle, and hybrid forms.
The dataset contains information on precipitation amount and type for the period of February 7 to March 29 2019 in Laret.

disdrometer

display_name: disdrometer

hydrometeors

display_name: hydrometeors

visibility

display_name: visibility


maintainer: Alexandre Buttler

en: biogeochemisty climate-change transplantation-experiment soil silvopastoral-systems

en: Dataset

en: Zip archive of all datasets detailed in study.

en:
Silvopastoral systems are highly productive and combine long-term wood production with annual provision of forage for livestock. In the Swiss Jura Mountains these systems are a key component of the landscape. As in other cold biomes, climate change can potentially accelerate landscape change within these historically sustainable systems.

In order to anticipate the evolution of subalpine wooded pasture ecosystems under future climate and land-use changes, this project focused on the interplay between soil, vegetation and climate. It was aimed at providing experimental evidence for chief ecosystem processes, with emphasis on the quality of the ecosystem services provided. The main interest was placed on vegetation turf resistance to climate change along an unwooded – sparsely wooded - densely wooded pasture gradient (land-use intensity), where plant productivity, diversity and succession along with rates of carbon cycling and microbial activity provided measures of ecosystem functioning at both plot and landscape level.

Experimental transplantation of monolith soil turfs to lower altitudes allowed to simulate soil warming and reduced annual precipitation. In order to simulate a year-round warmer and drier climate the natural climate variation along an altitudinal gradient was used as a proxy. The aim was to simulate realistic climate change scenarios for the second half of the 21st century predicted by the IPCC report and downscaled for Switzerland providing regionalized interpolated projections integrating therein trends for temperature increase and precipitation decrease. By using permanent meteorological stations within the network of the Federal Office of Meteorology and Climatology (MeteoSwiss), we obtained high resolution regional data on the variation of mean annual temperature (MAT) and mean annual precipitation (MAP) in relation to altitude in the Swiss Jura Mountains. We observed a general increase of +0.5 K in MAT and a decrease of -20 % MAP for each 100 m decrease in altitude along the SE slope of the Swiss Jura Mountains. These relationships served for the selection of the transplantation sites such that in comparison to a control site at 1350 m a.s.l. (Combe des Amburnex, N 46°54’, E 6°23’) a +2 K MAT and -20 % MAP was achieved at 1010 m a.s.l. (Saint-George, N 46°52’, E 6°26’), a +4 K MAT and -40 % MAP at 570 m a.s.l., (Arboretum d’Aubonne, N 46°51’, E 6°37’), and a +5 K MAT and -50 % MAP at 395 m a.s.l. (Les Bois Chamblard, N 46°47’, E 6°41’). The two stations at 1010 m a.s.l. and 570 m a.s.l. corresponded to the IPCC scenario A1B for a moderate increase in greenhouse gas emissions and to scenario A2 for a high increase in greenhouse gas emissions, respectively. The station at 395 m a.s.l. was chosen to represent an extreme scenario with climate variables lying at the positive tail distribution of model predictions under the A2 scenario.

Soil cores were assembled into rectangular PVC boxes of 60  80 cm2 size and of 35 cm height. All mesocosms were dug down to surface level into previously prepared trenches in the ground thus preventing lateral heat exchange with the atmosphere. Since at each site the mesocosms were placed in a common garden with no light interception, mesocosms with turfs from the two wooded pastures were shaded from direct sun light to simulate the natural light conditions in the corresponding habitats. Each mesocosm was equipped with a drainage system and was connected to a water tank thus representing a zero potential lysimeter collecting soil solution and precipitation/snowmelt runoff. ECH2O EC-TM sensor probes coupled to Em50 data-loggers (Decagon Devices, Inc., USA) recorded soil temperature and volumetric water content in each mesocosm at the top-soil (0 to -3 cm) every minute and data were averaged over one hour intervals. Climate parameters at each transplantation site were monitored continuously throughout the experiment by means of automated weather stations (Sensor Scope Sàrl, Switzerland), measuring rain precipitation (non-heated tipping bucket gauges) and air temperature and humidity 2 m above the ground surface at one minute intervals.

A list of above- and belowground variables were measured to assess the resilience of biogeochemical processes, plant productivity, tree regeneration, and carbon sequestration for each respective land-use practice. Furthermore, the experimental data were used to improve on (parameterization) the existing spatially explicit, dynamic model WoodPaM and refine the modelʼs climatic and land-use variables so that different scenarios of climate change and land use change could be simulated. Natural and management induced disturbance patterns were incorporated into the model.
The data have been made available within the project CCES Mounted. The climate stations Sensorscope are still in use within the project CLIMARBRE (Wald und Klimawandel, WSL/BAFU).

#References

1. Puissant, J., Cécillon, L., Mills, R.T.E., Robroek, B.J.M. Gavazov, K., De Danieli, S., Spiegelberger, T., Buttler, A., Brun, J.J. 2015. Seasonal influence of climate manipulation on microbial community structure and function in mountain soils. Soil Biology and Biochemistry 80: 296–305.
2. Mills, R., K. Gavazov, T. Spiegelberger, D. Johnson and A. Buttler 2014. Diminished soil functions occur under simulated climate change in a sup-alpine pasture, but heterotrophic temperature sensitivity indicates microbial resilience. Science of the Total Environment, vol. 473–474(0): 465-472.
3. Gavazov, K., Spiegelberger, T. and Buttler, A. 2014. Transplantation of subalpine wood-pasture turfs along a natural climatic gradient reveals lower resistance of unwooded pastures to climate change compared to wooded ones. Oecologia (174) : 1425-1435.
4. Peringer A., Siehoff S., Chételat J., Spiegelberger T., Buttler A. & Gillet F. 2013. Past and future landscape dynamics in pasture-woodlands of the Swiss Jura Mountains under climate change. Ecology and Society, 18, 3: 11. DOI: 10.5751/ES-05600-180311. [online] URL: http://www.ecologyandsociety.org/vol18/iss3/art11/
5. Gavazov, K. S., A. Peringer, A. Buttler, F. Gillet and T. Spiegelberger. 2013. Dynamics of Forage Production in Pasture-woodlands of the Swiss Jura Mountains under Projected Climate Change Scenarios. Ecology and Society 18 (1): 38. [online] URL: http://www.ecologyandsociety.org/vol18/iss1/art38/

biogeochemisty

display_name: biogeochemisty

silvopastoral-systems

display_name: silvopastoral-systems

soil

display_name: soil

transplantation-experiment

display_name: transplantation-experiment

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Biogeochemical data from a transplantation experiment of monolith soil turfs along an altitudinal gradient to simulate climate change scenarios


en: visual-attractiveness adults-and-teenagers online-questionnaire-survey perception-of-ecosystem-services nfi-photos

en: Questionnaire French

media_type: .pdf

en: Questionnaire Italian

en: SPSS-Dataset

en: Questionnaire German

en: Codebook to SPSS-file

en: Dataset in csv-format

en: This questionnaire survey was conducted as an online survey and aimed at investigating the relationship between physical forest characteristics, visual attractiveness of forest and the perception of ecological and cultural ecosystem services in urban forests. Each participant was shown 6 photos out of a pool of 50 photos taken from the Swiss National Forest Inventory (NFI) database. Physical forest characteristics were derived from the photos. The study was conducted as part of the "WaMos meets LFI" (WML) project.

adults-and-teenagers

display_name: adults-and-teenagers

nfi-photos

display_name: nfi-photos

online-questionnaire-survey

display_name: online-questionnaire-survey

perception-of-ecosystem-services

display_name: perception-of-ecosystem-services

visual-attractiveness

display_name: visual-attractiveness

en: Relationship between physical forest characteristics, visual attractiveness and perception of ecosystem services in urban forests


maintainer: Vanessa Burg

en: biogas switzerland bioenergy manure review

en: Biogas from animal manure in Switzerland: energy potential, technology development and resource mobilization

en: Aim of this white paper is to provide decision-makers, administrations and stakeholders with the most current research findings in order to promote the optimal use of bioenergy from manure in the Swiss energy transition. For this purpose, the results of the Swiss competence center for bioenergy research - SCCER BIOSWEET - are summarized and presented in a broader context. If nothing else is mentioned, the results refer to Switzerland and in case of the feedstock to the domestic biomass potentials.

bioenergy

display_name: bioenergy

biogas

display_name: biogas

manure

display_name: manure

review

display_name: review

switzerland

display_name: switzerland


en: gridded-data core geodata video-encoding sentinel-2 raster time-series

media_type: .sh

en:
Shell script to extract data from the CORE format. You can 1) download and unzip the file, 2) make the script executable and 3) run it.
Disclaimer: only provided for peer review. Proper software will become available in time on https://github.com/EnviDat/?q=core (both Web/JS for Frontend and Python for Backend).

en: CORE Dataset (80 GB)

media_type: CORE

en:
Shell script to reproduce the CORE format. You can 1) download and unzip the file, 2) make the script executable and 3) run it.
Disclaimer: only provided for peer review. Proper software for working with CORE will become available in time on https://github.com/EnviDat/?q=core (both Web/JS for Frontend and Python for Backend).

en:
DISCLAIMER: CORE is still in development. Interested parties are warmly invited to join common development, to comment, discuss, find bugs, etc.

Acknowledgement: The CORE format was proudly inspired by the Cloud Optimized GeoTIFF ([COG](https://www.cogeo.org/)) format, by considering how to leverage the ability of clients issuing HTTP GET range requests for a time-series of remote sensing and aerial imagery (instead of just one image).

License: The Cloud Optimized Raster Encoding (CORE) specifications are released to the public domain under a Creative Commons 4.0 CC0 "No Rights Reserved" international license. You can reuse the information contained herein in any way you want, for any purposes and without restrictions.

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Summary:
The Cloud Optimized Raster Encoding (CORE) format is being developed for the efficient storage and management of gridded data by applying video encoding algorithms. It is mainly designed for the exchange and preservation of large time series data in environmental data repositories, while in the same time enabling more efficient workflows on the cloud. It can be applied to any large number of similar (in pixel size and image dimensions) raster data layers. CORE is not designed to replace COG but to work together with COG for a collection of many layers (e.g. by offering a fast preview of layers when switching between layers of a time series).

WARNING: Currently only applicable to RGB/Byte imagery. The final CORE specifications may probably be very different from what is written herein or CORE may not ever become productive due to a myriad of reasons (see also 'Major issues to be solved').

With this early public sharing of the format we explicitly support the Open Science agenda, which implies "shifting from the standard practices of publishing research results in scientific publications towards sharing and using all available knowledge at an earlier stage in the research process" (quote from: European Commission, Directorate General for Research and Innovation, 2016. Open innovation, open science, open to the world).

CORE Specifications:

1) a MP4 or WebM video digital multimedia container format (or any future video container playable as HTML video in major browsers)

2) a free to use or open video compression codec such as H.264, VP9, or AV1 (or any future video codec that is open sourced or free to use for end users)

Note: H.264 is currently recommended because of the wide usage with support in all major browsers, fast encoding due to acceleration in hardware (which is currently not the case for AV1 or VP9) and the fact that MPEG LA has allowed the free use for streaming video that is free to the end users. However, please note that H.264 is restricted by patents and its use in proprietary or commercial software requires the payment of royalties to [MPEG LA](https://www.mpegla.com/programs/avc-h-264/). However, when AV1 matures and accelerated hardware encoding becomes available, AV1 is expected to offer 30% to 50% smaller file size in comparison with H.264, while retaining the [same quality](https://trac.ffmpeg.org/wiki/Encode/AV1).

3) the encoding frame rate should be of one frame per second (fps) with each layer segmented in internal tiles, similar to COG, ordered by the main use case when accessing the data: either layer contiguous or tile contiguous;

Note: The internal tile arrangement should support an easy navigation inside the CORE video format, depending on the use case.

4) a CORE file is optimised for streaming with the moov atom at the beginning of the file (e.g. with -movflags faststart) and optional additional optimisations depending on the codec used (e.g. -tune fastdecode -tune zerolatency for H.264)

5) metadata tags inside the moov atom for describing and using geographic image data (that are preferably compatible with the [OGC GeoTIFF standard](https://www.ogc.org/standards/geotiff) or any future standard accepted by the geospatial community) as well as list of original file names corresponding to each CORE layer

6) it needs to encode similar source rasters (such as time series of rasters with the same extent and resolution, or different tiles of the same product; each input raster should be having the same image and pixel size)

7) it provides a mechanism for addressing and requesting overviews (lower resolution data) for a fast display in web browser depending on the map scale (currently external overviews)

Major issues to be solved:

- Internal overviews (similar to COG), by chaining lower resolution videos in the same MP4 container for fast access to overviews first); Currently, overviews are kept as separate files, as external overviews.

- Metadata encoding (how to best encode spatial extent, layer names, and so on, for each of the layer inside the series, which may have a different geographical extent, etc...; Known issues: adding too many tags with FFmpeg which are not part of the standard MP4 moov atom; metadata tags have a limited string length.

- Applicability beyond RGB/Byte datasets; defining a standard way of converting cell values from Int16/UInt16/UInt32/Int32/Float32/Float64/ data types into multi-band Byte values (and reconstructing them back to the original data type within acceptable thresholds)

Example

Notice: The provided CORE (.mp4) examples contain modified Copernicus Sentinel data [2018-2021].

For generating the CORE examples provided, 50 original Sentinel 2 (S-2) TCI data images from an area located inside Switzerland were downloaded from www.copernicus.eu, and then transformed into CORE format using ffmpeg with H.264 encoding using the [x264 library](https://www.videolan.org/developers/x264.html).

For full reproducibility, we provide the original data set and results, as well scripts for data encoding and extraction (see resources).

core

display_name: core

geodata

display_name: geodata

gridded-data

display_name: gridded-data

raster

display_name: raster

time-series

display_name: time-series

video-encoding

display_name: video-encoding