en: snow-precipitation orographic-precipitation precipitation-variability cosmo-wrf complex-terrain

en: StationData

en: Snow depth maps over Dischma valley for 26 January 2016 and 9 March 2016 and snow depth differences between the two dates.

en:
The data presented here corresponds to the publication "Spatial variability in snow precipitation and accumulation in COSMO-WRF simulations and radar estimations over complex terrain" (Gerber et al., 2018a), which investigates the precipitation variability of snow precipitation in the central northern part of the Grisons (CH) and the publication "The importance of near-surface winter precipitation processes in complex alpine terrain" (Gerber et al., 2018b). The dataset contains:

* WRFsimulations: WRF simulation output for simulations with 4x (14x) terrain smoothing with an output timestep of 30 min/5 min and horizontal grid spacings of 1350 m, 450 m, 150 m and 50 m (currently: data available upon request).
* StationData: Meteorological station data of 18 meteorological stations in the central northern part of the Grisons with 30 minute resolution for the period 1 January 2016 till 1 May 2016.
* ADS80data: Photogrammetrically determined snow depth distribution data over the Dischma valley for the 26 January 2016 and 9 March 2016. Snow heights are corrected for buildings, vegetation (> 1m), outliers, and pixles, which are obivously snow-free on the pictures (Bühler et al., 2015). In addition the snow depth differences (snow depth on 9 March 2016 minus snow depth on 26 January 2016) are provided.

For more details about the simulation and observation data, see Gerber et al., 2018 and Gerber and Sharma (2018).

Publications:

Bühler, Y., Marty, M., Egli, L., Veitinger, J., Jonas, T., Thee, P., and Ginzler, C.: Snow depth mapping in high-alpine catchments using digital photogrammetry. Cryosphere, 9, 229–243, doi:10.5194/tc-9-229-2015, 2015.

Gerber, F., Besic, N., Sharma, V., Mott, R., Daniels, M., Gabella, M., Berne, A., Germann, U., and Lehning, M.: Spatial variability in snow precipitation and accumulation in COSMO-WRF simulations and radar estimations over complex terrain, The Cryosphere, 12, 3137–3160, doi:10.5194/tc-12-3137-2018, 2018.

Gerber, F., Mott, R. and Lehning, M.: The importance of near-surface winter precipitation processes in complex alpine terrain, Journal of Hydrometeorology, accepted, 2018.

Gerber, F., and Sharma, V.: Running COSMO-WRF on very-high resolution over complex terrain. Laboratory of Cryospheric Sciences CRYOS, École Polytechnique Fédérale de Lausanne EPFL, Lausanne, Switzerland. doi:10.16904/envidat.35, 2018.

complex-terrain

display_name: complex-terrain

cosmo-wrf

display_name: cosmo-wrf

orographic-precipitation

display_name: orographic-precipitation

precipitation-variability

display_name: precipitation-variability

snow-precipitation

display_name: snow-precipitation

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: DISCHMEX - High-resolution WRF simulations in complex alpine terrain and station measurements


maintainer: Jana von Freyberg

en: root-zone review uncertainties ecohydrology plant-water-uptake stable-water-isotopes endmember-mixing-models

state: active

type: dataset

en: Data set of MEE-20-04-264

media_type: application/pdf

en: The following two tables contain information about the data sources of the values reported in Table 1 and 2 in the paper “Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources” published in the journal ‘Methods in Ecology and Evolution’.

format: PDF

mimetype: application/pdf

en: Data set of MEE-20-04-264 (CSV)

media_type: CSV

format: CSV

en: The following two tables contain information about the data sources of the values reported in Table 1 and 2 in the paper “Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources” published in the journal 'Methods in Ecology and Evolution'.

ecohydrology

display_name: ecohydrology

endmember-mixing-models

display_name: endmember-mixing-models

plant-water-uptake

display_name: plant-water-uptake

review

display_name: review

root-zone

display_name: root-zone

stable-water-isotopes

display_name: stable-water-isotopes

uncertainties

display_name: uncertainties

education

fr: Education, science

de: Bildung, Wissenschaft

it: Formazione e scienza

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: Data set of: Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources


maintainer: EnviDat Support

en: compression-experiments snow-mechanics x-ray-tomography snow microstructure

en: README

media_type: TXT

en: README file explaining the organization of the data

format: TXT

en: Snowmicroquakes Data

en: Compression experiments, X-ray images and simulation data with supplemented README file

format: SERVICE

en:
When snow is compressed with a certain speed, micro-snowquakes are triggered in the porous structure of bonded crystals. The present dataset covers uniaxial compression experiments of snow at different strain rates and concurrent X-ray tomography imaging documenting this feature.

The experiments were conducted in a micro-compression stage operated in the X-ray tomography scanner in the SLF cold laboratory. The dataset comprises the compression force data of 17 compression experiments, the 3D image data from 4 X-ray tomography scans and the results of numerical simulations.

compression-experiments

display_name: compression-experiments

microstructure

display_name: microstructure

snow

display_name: snow

snow-mechanics

display_name: snow-mechanics

x-ray-tomography

display_name: x-ray-tomography

en: Compressive stick slip and snow-micro-quakes


en: shortwave-radiation precipitation-amount humidity longwave-radiation snow-depth air-temperature

en: Latest data from Stillberg climate station

media_type: SMET

en: This dataset represents the latest data from the Stillberg climate station updated daily. Automated quality control procedures are applied but no manual checks as in the main dataset.

en: Overview plot

media_type: PNG

en: Overview plots of all variables in timeseries.

format: PNG

en: Description of station parameters and history.

en: Site photo 3

media_type: JPEG

en: STB2 CSV

media_type: text/csv

en: Stillberg meteo as CSV (see smet for metadata header)

mimetype: text/csv

en: Site photo 2

en: Site photo 1

en: Snow sensor perpendicular to slope.

en: DATA ACCESS

en: Meteorological station data 1975-2016. Outlier analysis and general quality control has been carried out. Station was completely renovated in 2007 and instruments changed. A complete air temperature timeseries can be obtained by combining parameters "TA" and "AIR_TEMP_FUEHLER".

en: Longterm meteorological and snow station on a steep (c. 40 degrees) North-East exposed slope at 2090 m a.s.l above Davos, Switzerland. Established 1975 and ongoing measurements made, although instruments have changed during this period. The station is located within a experimental plantation that serves as avalanche protection to downslope communities as well as a unique site for treeline dynamics research.

air-temperature

display_name: air-temperature

humidity

display_name: humidity

longwave-radiation

display_name: longwave-radiation

precipitation-amount

display_name: precipitation-amount

shortwave-radiation

display_name: shortwave-radiation

snow-depth

display_name: snow-depth

education

en: Education and science

en: Long-term meteorological and snow station at 2090 m a.s.l., Stillberg, Davos, Switzerland (1975 - present)


en: viscous-deformation snow-failure snow sintering snow-avalanche fiber-bundle-model acoustic-emissions snow-mechanics

en: fiber-bundle-model-for-snow-failure

media_type: application/zip

en: Data underlying Figures in publication "Modelling Snow Failure Behavior and Concurrent Acoustic Emissions Signatures with a Fiber Bundle Model" submitted to "Geophysical Research Letters"

format: ZIP

mimetype: application/zip

en:
This dataset contains modeled and experimental results for laboratory snow failure experiments and the concurrent acoustic emissions signatures for different loading rates. For modelling the snow failure we used a fiber bundle model that includes sintering and viscous deformation.

The data underlay the figures in the publication "Modelling Snow Failure Behavior and Concurrent Acoustic Emissions Signatures with a Fiber Bundle Model" submitted for publication to "Geophysical Research Letters".

acoustic-emissions

display_name: acoustic-emissions

fiber-bundle-model

display_name: fiber-bundle-model

sintering

display_name: sintering

snow-avalanche

display_name: snow-avalanche

snow-failure

display_name: snow-failure

viscous-deformation

display_name: viscous-deformation

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Fiber Bundle Model for snow failure and concurrent Acoustic Emissions


maintainer: Sofia Pagliarin

en: imperviousness copernicus milan urban-atlas barcelona soil-sealing

media_type: mpk

en: Shareable project in Esri ArcGIS format including the HRL IMD data in raster format for each of the territorial boundaries as specified in letter a)-h), see Dataset description

media_type: .csv

en: Information on data sources

en:
Dataset description

This dataset is a recalculation of the Copernicus 2015 high resolution layer (HRL) of imperviousness density data (IMD) at different spatial/territorial scales for the case studies of Barcelona and Milan. The selected spatial/territorial scales are the following:

 * a)	Barcelona city boundaries
 * b)	Barcelona metropolitan area, Àrea Metropolitana de Barcelona (AMB)
 * c)	Barcelona greater city (Urban Atlas)
 * d)	Barcelona functional urban area (Urban Atlas)
 * e)	Milan city boundaries
 * f)	Milan metropolitan area, Piano Intercomunale Milanese (PIM)
 * g)	Milan greater city (Urban Atlas)
 * h)	Milan functional urban area (Urban Atlas)

In each of the spatial/territorial scales listed above, the number of 20x20mt cells corresponding to each of the 101 values of imperviousness (0-100% soil sealing: 0% means fully non-sealed area; 100% means fully sealed area) is provided, as well as the converted measure into squared kilometres (km2).

 Dataset composition

The dataset is provided in .csv format and is composed of:

_IMD15_BCN_MI_Sources.csv_: Information on data sources

 _IMD15_BCN.csv_: This file refers to the 2015 high resolution layer of imperviousness density (IMD) for the selected territorial/spatial scales in Barcelona:
 * a)	Barcelona city boundaries (label: bcn_city)
 * b)	Barcelona metropolitan area, Àrea metropolitana de Barcelona (AMB) (label: bcn_amb)
 * c)	Barcelona greater city (Urban Atlas) (label: bcn_grc)
 * d)	Barcelona functional urban area (Urban Atlas) (label: bcn_fua)

 _IMD15_MI.csv_: This file refers to the 2015 high resolution layer of imperviousness density (IMD) for the selected territorial/spatial scales in Milan:
 * e)	Milan city boundaries (label: mi_city)
 * f)	Milan metropolitan area, Piano intercomunale milanese (PIM) (label: mi_pim)
 * g)	Milan greater city (Urban Atlas) (label: mi_grc)
 * h)	Milan functional urban area (Urban Atlas) (label: mi_fua)

_IMD15_BCN_MI.mpk_: the shareable project in Esri ArcGIS format including the HRL IMD data in raster format for each of the territorial boundaries as specified in letter a)-h).

Regarding the territorial scale as per letter f), the list of municipalities included in the Milan metropolitan area in 2016 was provided to me in 2016 from a person working at the PIM.

In the IMD15_BCN.csv and IMD15_MI.csv, the following columns are included:

 * Level: the territorial level as defined above (a)-d) for Barcelona and e)-h) for Milan);
 * Value: the 101 values of imperviousness density expressed as a percentage of soil sealing (0-100%: 0% means fully non-sealed area; 100% means fully sealed area);
 * Count: the number of 20x20mt cells corresponding to a certain percentage of soil sealing or imperviousness;
 * Km2: the conversion of the 20x20mt cells into squared kilometres (km2) to facilitate the use of the dataset.

 Further information on the Dataset

This dataset is the result of a combination between different databases of different types and that have been downloaded from different sources. Below, I describe the main steps in data management that resulted in the production of the dataset in an Esri ArcGIS (ArcMap, Version 10.7) project.

 1. The high resolution layer (HRL) of the imperviousness density data (IMD) for 2015 has been downloaded from the official website of Copernicus. At the time of producing the dataset (April/May 2021), the 2018 version of the IMD HRL database was not yet validated, so the 2015 version was chosen instead. The type of this dataset is raster.

 2. For both Barcelona and Milan, shapefiles of their administrative boundaries have been downloaded from official sources, i.e. the ISTAT (Italian National Statistical Institute) and the ICGC (Catalan Institute for Cartography and Geology). These files have been reprojected to match the IMD HRL projection, i.e. ETRS 1989 LAEA.

 3. Urban Atlas (UA) boundaries for the Greater Cities (GRC) and Functional Urban Areas (FUA) of Barcelona and Milan have been checked and reconstructed in Esri ArcGIS from the administrative boundaries files by using a Eurostat correspondence table. This is because at the time of the dataset creation (April/May 2021), the 2018 Urban Atlas shapefiles for these two cities were not fully updated or validated on the Copernicus Urban Atlas website. Therefore, I had to re-create the GRC and FUA boundaries by using the Eurostat correspondence table as an alternative (but still official) data source. The use of the Eurostat correspondence table with the codes and names of municipalities was also useful to detect discrepancies, basically stemming from changes in municipality names and codes and that created inconsistent spatial features. When detected, these discrepancies have been checked with the ISTAT and ICGC offices in charge of producing Urban Atlas data before the final GRC and FUA boundaries were defined.

Steps 2) and 3) were the most time consuming, because they required other tools to be used in Esri ArcGIS, like spatial joins and geoprocessing tools for shapefiles (in particular dissolve and area re-calculator in editing sessions) for each of the spatial/territorial scales as indicated in letters a)-h).

Once the databases for both Barcelona and Milan as described in points 2) and 3) were ready (uploaded in Esri ArcGIS, reprojected and their correctness checked), they have been ‘crossed’ (i.e. clipped) with the IMD HRL as described in point 1) and a specific raster for each territorial level has been calculated. The procedure in Esri ArcGIS was the following:

 * Clipping: Arctoolbox > Data management tools > Raster > Raster Processing > Clip. The ‘input’ file is the HRL IMD raster file as described in point 1) and the ‘output’ file is each of the spatial/territorial files. The option "Use Input Features for Clipping Geometry (optional)” was selected for each of the clipping.
 * Delete and create raster attribute table: Once the clipping has been done, the raster has to be recalculated first through Arctoolbox > Data management tools > Raster > Raster properties > Delete Raster Attribute Table and then through Arctoolbox > Data management tools > Raster > Raster properties > Build Raster Attribute Table; the "overwrite" option has been selected.

 Other tools used for the raster files in Esri ArcGIS have been the spatial analyst tools (in particular, Zonal > Zonal Statistics). As an additional check, the colour scheme of each of the newly created raster for each of the spatial/territorial attributes as per letters a)-h) above has been changed to check the consistency of its overlay with the original HRL IMD file. However, a perfect match between the shapefiles as per letters a)-h) and the raster files could not be achieved since the raster files are composed of 20x20mt cells.

The newly created attribute tables of each of the raster files have been exported and saved as .txt files. These .txt files have then been copied in the excel corresponding to the final published dataset.

barcelona

display_name: barcelona

copernicus

display_name: copernicus

imperviousness

display_name: imperviousness

milan

display_name: milan

soil-sealing

display_name: soil-sealing

urban-atlas

display_name: urban-atlas

en: Soil sealing Barcelona and Milan different territorial levels


wind-crust-formation-snowmicropen-data

title_for_slug: wind-crust-formation-snowmicropen-data

en: snowmicropen wind-crust wind-packing smp snow

en: SMP data

media_type: Link

en: Links to the dataset

en: README

media_type: text/plain

en: Describes the dataset in detail

mimetype: text/plain

en: Processing scripts

en: Contains the R-scripts used to process the SMPs

en:
This dataset contains the SnowMicroPen (SMP) data from 38 wind tunnel experiments on wind-packing / wind crust formation. These experiments were performed in the winters 2015/16 and 2016/17 and include more than 1000 SMP measurements.

The SMPs are organized per experiment. Each experiment subfolder contains the processed SMP profiles and some additional files.

Please refer to the README for more details on the data. The processing scripts are available for download as well. The scripts are mainly provided as documentation and would need to be adjusted to be used.

This dataset is the basis of the following publication:

Sommer C.G., Lehning M., & Fierz C. (2017). Wind tunnel experiments: Saltation is necessary for wind-packing. Journal of Glaciology, 63(242), 950-958. doi:10.1017/jog.2017.53

smp

display_name: smp

snow

display_name: snow

snowmicropen

display_name: snowmicropen

wind-crust

display_name: wind-crust

wind-packing

display_name: wind-packing

en: Wind crust formation: SnowMicroPen data


maintainer: Matthias Haeni

en: swiss-forest-lab climate-change temperature meteorology relative-humidity meteo long-term precipitation par wind-speed

en: Forest station data (ALB)

en: Site aerial photo (Alpthal)

media_type: image/jpeg

en: Site aerial photo from the Alpthal forest station. Source: Google. 2016. Site aerial photo from the Alpthal forest station. Retrieved November 11, 2016, from https://www.google.ch/maps

mimetype: image/jpeg

en: Data plot of the forest station (ALB)

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for one meteorological station in Alpthal in Switzerland which is located within a natural coniferous forest (ALB) with Norway spruce (_Picea abies_; 180-230 yrs) as dominant tree species. The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Alpthal is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: Alpthal, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


causal-effect-of-lup

title_for_slug: causal-effect-of-lup

en: annual-effect plan-evaluation psm-did land-use-change land-use-planning

en: causal-effect-of-lup

media_type: ZIP

en:
Data.dbf contains the varibles of 1662 villages in Zhangzhou Cities
PSM_DID.R can re-produce the results

en:
Title: Does zoning contain built-up land expansion? Causal evidence from Zhangzhou City, China.

Research objective: Built-up land zoning is an imporatant policy measure of land use planning (LUP) to contain built-up land expansion in China. We used a difference-indifference model with propensity score matching to estimate the average and annual effect of built-up land zoning on built-up land expansion in Zhangzhou City, China between 2010 and 2020.

Data: Data.dbf contains the varibles of 1662 villages in Zhangzhou Cities in 1995, 2000, 2005, 2010, 2013, 2015, 2018, and 2020.
XZQDM2 is villages' unique administrative ID;
Area is the land area of village i;
Dis2water is the Euclidean distance from village i to the nearest waterbody;
Dis2coastl is the Euclidean distance from village i to the nearest coastline;
Dis2city is the the Euclidean distance from village i to the city center;
Dis2county is the the Euclidean distance from village i to the nearest county center;
Elevation is the the average elevation within village i;
Dis2road is the the Euclidean distance from village i to the nearest road;
Nei_Built_ is the the area of built-up land (Nei Built.upit) in the neighboring villages of village i in year t;
Treated is a binary variable, Treated = 1 to the villages that were partially or entirely located inside the development-permitted zones, and Treated = 0 to the villages that were entirely located outside the development-permitted zones;
Intensity is the percentage of land that was assigned to the development-permitted zones in village i;
Year represent the year in 1995, 2000, 2005, 2010, 2013, 2015, 2018, and 2020;
BuLE is the dependent variable, representing built-up land expansion in village i in year t;
Town is town' unique administrative ID.

Method: First, we employed propensity score matching to overcome the selection bias and satisfy the parallel trend
assumption. Second, we built four Difference-in-Difference models to estimate the average and annual effect.

annual-effect

display_name: annual-effect

land-use-change

display_name: land-use-change

land-use-planning

display_name: land-use-planning

plan-evaluation

display_name: plan-evaluation

psm-did

display_name: psm-did

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Causal effect of LUP


en: hydrology model discharge climate-change-impact water-temperature

en: Data access

media_type: .zip

en:
This work presents the first extensive study of climate change impacts on rivers temperature in Switzerland. Results show that even for low emissions scenarios, water temperature increase will lead to adverse effect for both ecosystems and socioeconomic sectors (such as nuclear plant cooling) throughout the 21st century. For high emissions scenarios, the effect will be worsen. This study also shows that water warming in summer will be more important in Alpine regions than in lowlands.
This material is distributed under CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/legalcode).

climate-change-impact

display_name: climate-change-impact

discharge

display_name: discharge

hydrology

display_name: hydrology

model

display_name: model

water-temperature

display_name: water-temperature

en: Dataset for: Future water temperature of rivers in Switzerland under climate change investigated with physics-based models


maintainer: Sebastian Schlögl

en: snow stable-boundary-layer atmosphere atmospheric-turbulence turbulent-sensible-heat-fluxes

en: Turbulence data WFJ11

en: Turbulence data GR00

en: sensor in 2 m height

en: Turbulence data AA00

en: sensor in 1 m height

en: Turbulence data PM07

en: NWW

en: Turbulence data WFJ07

en: lower sensor

en: upper sensor

en: SEE

en: sensor in 0.2 m height

en: sensor in 25 m height

en:
We used five different atmospheric turbulence datasets from four test sites, with these sites showing differences in their topographical characteristics. We chose one typical alpine test site with high topographical complexity (Weissfluhjoch, Davos, Switzerland) and three test sites consisting of one glacier site (Plaine Morte, Crans-Montana, Switzerland) and two polar sites (Greenland and Antarctica) representing a quasi-ideal site with homogeneous surface and quasi infinite fetch in all directions.
The turbulent sensible heat flux was calculated using the eddy-covariance method. Note that the sonic temperature fluctuations have been converted into virtual temperature fluctuations.
Three-dimensional wind velocity and air temperature were processed using a linear detrending (Rannik and Vesala, 1999) and a planar fit approach (Massmann and Lee, 2002) to rotate the coordinate system. Air temperature, relative humidity and air pressure from weather stations were used to calculate air properties, which are required for the data processing. The weather stations are located in the immediate vicinity of the turbulence tower and are affected by the same air masses. Turbulence data were averaged to 30-min intervals, whilst changing to a 15-min time interval marginally affects the heat fluxes at the Weissfluhjoch test site (Mott et al., 2011). Note that we define a negative sensible heat flux as being directed towards the snow surface and a positive sensible heat flux as being directed upwards.
The selected datasets and corresponding test sites are briefly introduced below:

Weissfluhjoch 2007 (WFJ07): A vertical set-up of two three-dimensional ultrasonic anemometers (CSAT3, Campbell Scientific, Inc.) was used at the traditional field site Weissfluhjoch (2540 m asl.) to measure three-dimensional wind velocity and air temperature at a frequency of 20 Hz. The sensors were mounted 3 m and 5 m above the ground and provided reliable data for 50 days between 11 February 2007 and 24 April 2007. Further information on the field campaign can be found in Stössel et al. (2010) and Mott et al. (2011).

Weissfluhjoch 2011-13 (WFJ11): Three-dimensional wind velocity and air temperature were recorded at 5 m above the ground at a frequency of 10 Hz with a three-dimensional ultrasonic anemometer (CSAT3). The analysis was conducted for data obtained between February and March in the years 2011-13.

Plaine Morte 2007 (PM07): Two three-dimensional ultrasonic anemometers (CSAT3) were installed on a horizontal boom facing opposite directions (west-north-west vs. east-south-east) at 3.75 m above the ground to measure air temperature and three-dimensional wind velocity at 20 Hz. The data were collected at the almost flat field site on the Plaine Morte glacier (2750 m asl.) near Crans-Montana, Switzerland from February to April 2007. High quality meteorological data were additionally recorded and used to force the model. A detailed description about the set-up at the Plaine Morte glacier can be found in Huwald et al. (2009) and Bou-Zeid et al. (2010).

Greenland 2000 (GR00): High-frequency three-dimensional ultrasonic anemometer measurements (CSAT3) were recorded at 50 Hz at the Summit Camp (72.3 °N, 38.8 °W, 3208 m asl.) located on the northern dome of the Greenland ice sheet. Data were collected at 1 m and 2 m above the snow surface during summer in 2000 and 2001. Additionally, meteorological measurements were obtained for the post processing and used to force the model. More information about the field campaign can be found in Cullen et al. (2007, 2014).

Antarctica 2000 (AA00): A set-up of three vertical three-dimensional ultrasonic anemometers (DA-600, Kaijo Denki) were installed at Mizuho Station (70°42' S, 44°20' E, 2230 m asl.) in Eastern Antarctica at 0.2, 1 and 25 m and recorded turbulence data at a frequency of 100 Hz from October to November 2000. Longwave and shortwave radiation, relative humidity, air and snow surface temperature were additionally measured and used to force the model. More information about the field campaign can be found in Nishimura and Nemoto (2005).

atmosphere

display_name: atmosphere

atmospheric-turbulence

display_name: atmospheric-turbulence

snow

display_name: snow

stable-boundary-layer

display_name: stable-boundary-layer

turbulent-sensible-heat-fluxes

display_name: turbulent-sensible-heat-fluxes

en: How do stability corrections perform in the stable boundary layer over snow?


en: snowpack meteorological-data catchment-data snow-hydrology rain-on-snow

en: The file contains all data needed to run simulations with the snow cover model SNOWPACK.

en: Meteorological data used to run SNOWPACK for 58 catchments in the Swiss Alps. The data consists of a 2 km grid of "virtual meteorological stations" for each catchment. It was used to simulate snow cover processes during rain-on-snow events, therefore meteorological data of each catchment contains at least one rain-on-snow event. Further information can be found in the attached readme.txt and in Würzer & Jonas et al. (2017), currently under review in Hydrological Processes.

catchment-data

display_name: catchment-data

meteorological-data

display_name: meteorological-data

rain-on-snow

display_name: rain-on-snow

snow-hydrology

display_name: snow-hydrology

snowpack

display_name: snowpack

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Meteorological data for investigation of rain-on-snow events in 58 catchments in Switzerland


maintainer: Anita Risch

en: grassland invertebrates grazing ecosystem-multifunctionality swiss-national-park subalpine ecosystem-functions exclusion ecosystem-coupling vertebrates

en: Datafile

en:
ID: Plot ID composited of Fence & Treatment number (90 plots total)

Grassland: Name of the six subalpine grasslands where the exclosure setups were erected. In large grasslands (GRIM, MING, STAB), we established four hierarchical exclosure setups (two in short- and two in tall-grass vegetation), whereas in the small grasslands (BOTS, DADA, MARG) only one setup was erected per vegetation type. BOTS = Val dal Botsch, GRIM = Grimmels, MING = Val Mingér, STAB = Alp Stabelchod, DADA = Stabelchod da daint, MARG = Margunet

Fence: Number of exclosure setup (1 - 18). Note that uneven numbers (1,3,5,7,9,11,13,15,17) represent setups established on short-grass vegetation, even numbers (2,4,6,8,10,12,14,16,18) setups established in tall-grass vegetation
Vegetation	Vegetation type, 1 = short-grass vegetation, 2 = tall-grass vegetation; (9 replicate fences on each vegetation type)
Treatment	Progressive animal exclusion, numbers indicate which animals had access to the plot: 1 = Large/medium/small mammals, and invertebrates have access ("L/M/S/I"), 2 = Medium/small mammals, and invertebrates have access (“M/S/I”), 3 = Small mammals and invertebrates have access (“S/I”), 4 = Invertebrates have access (“I”), 5 = No animals have access (“None”)

Bulkden:	Fine fraction soil bulk density (g cm-3)

Soil.moist	: Soil moisture (%)

Soil.org.C: Rhizosphere soil C content (%)

pH: Soil pH

Nema.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil nematode community composition

Nema.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil nematode community composition

Micro.PC1: 	First principal component (PC1) of principal component analysis (PCA) of the soil microbial community composition

Micro.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil microbial community composition

Abv.Inv.PC1: First principal component (PC1) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Abv.Inv.PC2: Second principal component (PC2) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Arthr.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil arthropod community composition

Arthr.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil arthropod community composition

Plant.PC1: First principal component (PC1) of principal component analysis (PCA) of the plant community composition

Plant.PC2: Second principal component (PC2) of principal component analysis (PCA) of the plant community composition

Root.biomass: Root biomass (g m-2)

Micro.biomass	: Microbial biomass carbon (mg kg-1)

Plant.richness: Plant species richness

Plant.N: Plant tissue nitrogen content (%)

Soil.N.min: Soil net nitrogen mineralisation (kg ha-1 season)

Soil.resp: Soil carbon dioxide emissions (soil respiration; g m-2 h-1)

format: XLS

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:
 > Risch AC, Ochoa-Hueso R, van der Putten WH, Bump JK, Busse MD, Frey B, Gwiazdowicz DJ, Page-Dumroese DS, Vandegehuchte ML, Zimmermann S, Schütz M. Size-dependent loss of aboveground animals differentially affects grassland ecosystem coupling and functions. 2018. Nature Communications 9: 3684. [doi: 10.1038/s41467-018-06105-4](https://doi.org/10.1038/s41467-018-06105-4).

Please cite this paper together with the citation for the datafile.

#Methods
Study sites
The experimental exclosure setups were installed within the SNP (IUCN category Ia preserve; Dudley 2008), in south-eastern Switzerland. The park covers 172 km2 of forests and subalpine and alpine grasslands along with scattered rock outcrops and scree slopes. The entire area has been protected from human impact (no hunting, fishing, camping or off-trail hiking) since 1914. Large, fairly homogenous patches of short- and tall-grass vegetation, which originate from different historical management and grazing regimes, cover the park’s subalpine grasslands entirely. Short-grass vegetation developed in areas where cattle used to rest (nutrient input) prior to the park’s foundation (14th century to 1914) (Schütz and others 2003, 2006) and is dominated by lawn grass species such as Festuca rubra L., Briza media L. and Agrostis capillaris L. (Schütz and others 2003, 2006). Today, this vegetation type is intensively grazed by diverse vertebrate and invertebrate communities that inhabit the park and consume up to 60% of the available biomass (Risch and others 2013). Tall-grass vegetation developed where cattle formerly grazed, but did not rest, and is dominated by rather nutrient-poor tussocks of Carex sempervirens Vill. and Nardus stricta L. (Schütz and others 2003, 2006). This vegetation type receives considerably less grazing, with only roughly 20% of the biomass consumed (Risch and others 2013). Consequently, the two vegetation types together represent a long-term trajectory of changes in grazing regimes. Underlying bedrock of all grasslands is dolomite, which renders these grasslands rather poor in nutrients regardless of former and current land-use regimes.
Experimental design
To progressively exclude aboveground vertebrate and invertebrate animals, we established 18 size-selective exclosure setups (nine in short-grass, nine in tall-grass vegetation) distributed over six subalpine grasslands across the SNP (Risch and others 2013, 2015). Elevation differences of exclosure locations did not exceed 350 m (between 1975 and 2300 m a.s.l.). The exclosures were established immediately after snowmelt in spring 2009 and were left in place for five consecutive growing seasons (until end of 2013). They were, however, temporarily dismantled every fall (late October after first snowfall) to protect them from avalanches. They were re-established in the same location every spring immediately after snowmelt. Each size-selective exclosure setup consisted of five plots (2 x 3 m) that progressively excluded aboveground vertebrates and invertebrates from large to small. The plots are labelled according to the guilds that had access to them “L/M/S/I”, “M/S/I”, “S/I”, “I”, “None”; L = large mammals, M = medium mammals, S = small mammals, I = invertebrates, None = no animals had access. As we only had permission to have the experimental setup in place for five consecutive growing seasons, the experiment had to be completely dismantled in the late fall of 2013 and all material removed from the SNP.
Our exclosure design was aimed at excluding mammalian herbivores, but naturally also excluded the few medium and small mammalian predators, as well as the entire aboveground invertebrate food web. A total of 26 large to small mammal species can be found in the SNP, but large apex predators are missing (wolf, bear, lynx) . Reptiles, amphibians and birds are scarce to absent in the subalpine grasslands under study. Only two reptile species occur in the park and they are confined to rocky areas that warm up enough for them to survive. One frog species spawns in an isolated pond far from our grasslands. Only three bird species occasionally feed on the subalpine grasslands. Using game cameras (Moultrie 6MP Game Spy I-60 Infrared Digital Game Camera, Moultrie Feeders, Alabaster, AL, USA), we did observe that the medium- and small-sized mammals (marmot/hares and mice) were not afraid to enter the fences and feed on their designated plots. We never spotted reptiles, amphibians or birds on camera. We distinguished between 59 higher aboveground-dwelling invertebrate taxa that our size-selective exclosures excluded (see also methods for aboveground-dwelling invertebrates below).
The “L/M/S/I” plot (not fenced) was located at least 5 m from the 2.1 m tall and 7 x 9 m large main electrical fence that enclosed the other four plots. The bottom wire of this fence was mounted at 0.5 m height and was not electrified to enable safe access for medium and small mammals, while fencing out the large ones. Within each main fence, we randomly established four 2 x 3 m plots separated by 1-m wide walkways from one another and from the main fence line: 1) the “M/S/I” plots were unfenced, allowing access to all but the large mammals; 2) the “S/I” plots (10 x 10 cm electrical mesh fence) excluded all medium-sized mammals. Note that the bottom 10 cm of this fence remained non-electrified to enable safe access for small mammals; 3) the “I” plots (2 x 2 cm metal mesh fence) excluded all mammals. We double-folded the mesh at the bottom 50 cm to reduce the mesh size to smaller than 1 x 1 cm openings; and 4) the “None” plots were surrounded by a 1 m tall mosquito net (1.5 x 2 mm) to exclude all animals. The top of the plot was covered with a mosquito-meshed wooden frame mounted to the corner posts (roof). We treated these plots a few times with biocompatible insecticide (Clean kill original, Eco Belle GmbH, Waldshut-Tiengen, Germany) to remove insects that might have entered during data collection or that hatched from the soil, but amounts were negligible and did not impact soil moisture conditions within these plots.
To assess whether the design of the “None” exclosure (mesh and roof) affected the response variables within the plots and, therefore, influenced the results, we established an additional six “micro-climate control” exclosures (one in each of the six grasslands) (Risch and others 2013, 2015). These exclosures were built as the “None” exclosures but were open at the bottom (20 cm) of the 3-m side of the fence facing away from the prevailing wind direction to allow invertebrates to enter. A 20-cm high and 3-m long strip of metal mesh was used to block access to small mammals. Thus, this construction allowed a comparable micro-climate to the “None” plots, but also a comparable feeding pressure by invertebrates to the “I” plots. We compared various properties within these exclosures against one another to assess if our construction altered the conditions in the “None” plots. We showed that differences in plant (e.g., vegetation height, aboveground biomass) and soil properties (e.g., soil temperature, moisture) found between the “I” and the “None” treatments were not due to the construction of the “None” exclosure, but a function of animal exclusions, although the amount of UV light reaching the plant canopy was significantly reduced (Risch and others 2013).
Aboveground invertebrate sampling
Aboveground invertebrates were sampled with two different methods to capture both ground- and plant-dwelling organisms: 1) we randomly placed two pitfall traps (67 mm in diameter, covered with a roof) filled with 20% propylene glycol in one 1 x 1 m subplot of the 2 x 3 m treatment plots in spring 2013 (May) and emptied them every two weeks until late September 2013 (Vandegehuchte and others 2017b, 2018). A pitfall trap consisted of a plastic cylinder (13 cm depth, 6.75 cm diameter). Within each cylinder we placed a 100 ml plastic vial with outer diameter 6.70 cm and on top of the cylinder we placed a plastic funnel to guide the invertebrates into the vials. Each trap was cover with a cone-shaped and transparent plastic roof to protect the trap from rain (Vandegehuchte and others 2017b, 2018). Note that in the “None” plots only one trap was placed as control to check for effectiveness of the exclosure. 2) We vacuumed all invertebrates from a 60 x 60 cm area on another 1 x 1 m subplot with a suction sampler (Vortis, Burkhard manufacturing CO, Ltd., Rickmansworth, Hertfordshire, UK) every month from June to September 2013 (Vandegehuchte and others 2017b, 2018). For this purpose, we quickly placed a square plastic frame (60 x 60 x 40 cm) with a closable mosquito mesh sleeve attached to the top edge into the plot from the outside. The suction sample was then inserted into through the sleeve and operated for 45 s to collect the invertebrates (Vandegehuchte and others 2017b, 2018).
We sorted the ≈100 000 individuals collected with both methods by hand and identified each individual morphologically to the lowest taxonomic level feasible (59 taxa, including orders, suborders, subfamilies, families; phylum for Mollusca). These taxa belonged to the following feeding types: 19 herbivores, 16 detritivores, 9 predators, 8 mixed feeders, 5 omnivores and 2 non-classified feeders (or not feeding as adults) (Vandegehuchte and others 2017b). We summed the numbers from the two pitfall traps and the suction sampling over the course of the 2013 season to represent the aboveground invertebrate abundance and community composition of a plot. Note: we did not specifically attempt to catch flying invertebrates with e.g., sticky traps, thus a few flying insects may have been missed with our vacuum sampling approach.
Sampling of plant properties
The vascular plant species composition was assessed at peak biomass every summer (July) by estimating the frequency of occurrence of each species with the pin count method in each plot (Frank and McNaughton 1990). A total of 172 taxa occurred within our 90 plots and we calculated plant species richness for each plot separately. We used the 2013 data in this study. Plant quality was assessed every year in July and September; here we use plant quality at the end of the experiment (September 2013). Two 10 x 100 cm wide strips of vegetation per plot were clipped, combined, dried at 65°C, and ground (Pulverisette 16, Fritsch, Idar-Oberstein, Germany) to pass through a 0.5 mm sieve. Twenty randomly selected samples across all treatments were analysed for N (Leco TruSpec Analyser, Leco, St. Joseph, Michigan, USA) (Vandegehuchte and others 2015). Nitrogen concentrations of the other samples were then estimated from models established for the experiment and the entire SNP relating Fourier transform-near infrared reflectance (FT-NIR) spectra to the measured values of N using a multi-purpose FT-NIR spectrometer (Bruker Optics, Fällanden, Switzerland) (Vandegehuchte and others 2015). Root biomass was sampled every fall by collecting five 2.2 cm diameter x 10 cm deep soil samples (Giddings Machine Company, Windsor, CO, USA) per plot (450 samples year-1). The samples were dried at 30 °C and roots were sorted from the sample by hand. We sorted each sample for 1 h which allowed to retrieve over 90% of all roots present in the samples (Risch and others 2013). The roots were then dried at 65 °C for 48 and weighed to the nearest mg. We averaged the values per plot and used the 2013 data only in this study.
Sampling of edaphic communities
In 2009, 2010, and 2011 we collected three composited soil samples (5 cm diameter x 10 cm depth; AMS Samplers, American Falls, ID, USA) and assessed bacterial community structure using T-RFLP profiling (Liu and others 1997; Blackwood and others 2003; Hodel and others 2014). We detected a total of 89 operational taxonomic units (OTUs). These values are in accordance with other studies reporting OTU richness (Wirthner and others 2011; Zumsteg and others 2012; Meola and others 2014) using T-RFLP profiling, a method that detects the most abundant, and thus likely, the most relevant, taxa. We averaged the data over the three years of collections for our calculations. Microbial biomass carbon (MBC) was determined with the substrate-induced method (Anderson and Domsch 1978) every fall (September) between 2009 and 2013 by collecting three mineral soil samples (5 cm diameter × 10 cm mineral soil core, AMS Samplers, American Falls, ID, USA). The three samples were combined (90 samples for each sampling year), immediately put on ice, taken to the laboratory, passed through a 2-mm sieve and stored at 4°C. Again, we only used the 2013 data in this study.
Soil samples (5 cm diameter x 10 cm depth) to extract soil arthropods were collected in June, July, and August 2011 with a soil corer lined with a plastic sleeve to ensure an undisturbed sample (total of 270 samples). The plastic line core was immediately sealed on both ends using cling film and put into a cooler. All plots were sampled within three days and the extraction of arthropods started the evening of the sampling day using a high-gradient Tullgren funnel apparatus (Crossley and Blair 1991; Vandegehuchte and others 2015). Samples were kept in the extractor for four days and the soil arthropods were collected in 95% ethanol. All individuals were counted and each individual was identified morphologically to the lowest level feasible [76 taxa, including orders, suborders, subfamilies, families (Protura, Thysanoptera, Aphidina, Psylina, Coleoptera, Brachycera, Nematocera, Auchenorryncha, Heteroptera, Formicidae); sub-phylum for Myriapoda, for Acari and Collembola also including morpho-species). Note that we also included larval stages (nine of the 76 taxa) (Vandegehuchte and others 2015). All data were summed over the season. A detailed species list for mites and collembolans is published (Vandegehuchte and others 2017a) [https://doi.org/10.1371/journal.pone.0118679.s001]. Earthworms are rare in the SNP and therefore were not included. We collected eight random 2.2 cm diameter x 10 cm deep soil cores from each plot in September 2013 to determine the soil nematode community composition. The samples were mixed and the nematodes were extracted from 100 ml of fresh soil using Oostenbrink elutriators (Oostenbrink 1960). All nematodes in a 1 ml of the 10 ml extract were counted, a minimum of 150 individuals sample-1 were identified to genus or family level using (Bongers 1988), the numbers of all nematodes were extrapolated to the entire sample and expressed for a 100 g dry sample. In total we identified 63 genus or family levels (Vandegehuchte and others 2015). The list of all the nematodes found is published (Vandegehuchte and others 2015) [http://www.oikosjournal.org/appendix/oik-03341] or DOI: [doi: 10.1111/oik.03341].
We are aware that sampling soil microbes from 2009 to 2011 and soil arthropods in 2011 was not ideal, but we are positive that this does not bias the results. Most of the parameters measured in our experiment either already showed a treatment response after the first growing season (e.g., plant biomass) or did not respond over the entire time experiment (e.g., microbial biomass C). The microbial community composition (2009 – 2011) was highly influenced by inter-annual differences in temperature and precipitation, but did not differ between treatments or vegetation types (Hodel and others 2014). We therefore felt comfortable using the 2009 through 2011 data for describing the soil microbial community in our experimental treatments. Similarly, we are positive that our soil arthropod data are representative. We did assess soil arthropods in August 2012 and found no differences to the August 2011 data. However, we did not feel comfortable combining the 2011 June, July, August data with only August data for 2012 for our analyses.
Sampling of soil properties
We collected three soil samples (5 cm diameter x 10 cm depth) in each plot in September 2013 after removing the vegetation. First, we collected the top layer of mineral soil rich in organic matter, the surface organic layer or rhizosphere, typically 1 to 3 cm in depth with a soil corer (AMS Samples, American Falls, Idaho, USA). Second, we collected a 10 cm mineral soil core beneath this surface layer. The cores for each layer were composited, dried at 65 °C for 48 h and fine-ground to pass a 0.5 mm screen. We then analysed all samples for total C using a Leco TruSpec Analyser (Leco, St. Joseph, Michigan, USA). Mineral soil pH was measured potentiometrically in 1:2 soil:CaCl2 solution with an equilibration time of 30 min.
Soil net N mineralisation was assessed during the 2013 growing season (Risch and others 2015). For this purpose, we randomly collected a 5 cm diameter x10 cm deep soil sample with a soil corer (AMS Samples, American Falls, Idaho, USA) after clipping the vegetation in June 2013. After weighing and sieving (4 mm mesh) the soil, we extracted a 20 g subsample in 1 mol l-1 KCl for 1.5 h on an end-over-end shaker and thereafter filtered it through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnenmühle FineArt GmbH, Dassel, Germany). From these filtrates NO3- concentrations were measured colorimetrically (Norman and Stucki 1981) and NH4+with flow injection analysis (FIAS 300, Perkin Elmer, Waltham Massachusetts, USA) (Risch and others 2015). We dried the rest of the sample 105 °C to constant mass to determine fine,fraction bulk density. A second soil sample was collected within each plot in June 2013 with a corer lined with a 5 x 13 cm aluminium cylinder. The corer was driven 11.5 cm deep into the soil so that the top 1.5 cm of the cylinder remained empty. Into this space we placed a polyester bag (250 µm) filled an ion-exchanger resin to capture the incoming N. The bag was filled with a 1:1 mixture of acidic and alkaline exchanger resin (ion-exchanger I KA/ion exchanger IIIAA, Merck AG, Darmstadt, Germany). We then removed 1.5 cm soil at the bottom of the cylinder and placed a second resin exchanger bag into this space to capture the N leached from the soil column. To assure that the exchange resin was saturated with H+ and Cl- prior to filling the bags, the mixture was stirred with 1.2 ml l-1 HCl for 1 h and then rinsed with demineralized water until the electrical conductivity of the water reached 5 µm cm-1. The cylinder with the resin bags in place was reinserted into the soil with the top flush to the soil surface and incubated for three months. We recollected the cylinders in September 2013. Each resin bag and 20 g of sieved soil (4 mm mesh) from each cylinder were then separately extracted with KCl and NO3- and NH4+ concentrations were measured. Nitrate and NH4+ concentrations of all samples were then converted to a content basis by multiplying their values with fine fraction bulk density. Net N mineralisation was thereafter calculated as the difference between the N content of the samples collected at the end of the three-month incubation (including the N extracted from the bottom resin bag) and the N content at the beginning of the incubation (Risch and others 2015).
Soil CO2 emissions were measured every two weeks between 0900 and 1700 hrs from early May through late September 2013 with a PP-Systems SRC-1 soil respiration chamber (15 cm high, 10 cm diameter; closed circuit) attached to a PP-Systems EGM-4 infrared gas analyser (PP-Systems, Amesbury, MA, USA) on two locations per plot (Risch and others 2013). The chamber was placed on randomly placed, permanently installed PVC collars (10 cm diameter) driven 5 cm into the soil at the beginning of the study (Risch and others 2013). Freshly germinated plants growing within the collars were removed prior to each measurement to avoid measuring plant respiration or photosynthesis. The two measurements collected per plot and sampling date were averaged.
Soil moisture (with time domain reflectometry; Field-Scout TDR-100, Spectrum Technologies, Plainfield, Illionois, USA) and temperature (with a waterproof digital pocket thermometer; Barnstead International, Dubuque, Iowa, USA) were measured at five random locations per plot every two weeks during the growing seasons during the experiment for the 0 to 10 cm depth (Risch and others 2013, 2015). As soil moisture and soil temperature were highly negatively correlated (Risch and others 2013), we only used soil moisture for this study. We used plot-level averages of all values available to capture soil moisture variability during the five years of the experiment. The results remained unchanged when we only used soil moisture from the 2013 growing season.
Numeral calculations and statistical analyses
Ecosystem coupling. We conducted principal component analyses (PCAs; unscaled) at the complete dataset level using the abundances of each taxonomical entity to describe each of the five different communities used in this study: aboveground-dwelling invertebrates, vascular plants, soil microorganisms, soil arthropods and soil nematodes. We retained the first two components (PCA axis 1 and PCA axis 2) of each analysis as we found them to adequately represent the temporal and spatial variability of our 90 treatment plots in previous studies55,67. Together they explained a total of 71.70% of the variation for aboveground invertebrates, 44.36% for plants, 44.85% for soil microorganisms, 61.85% for soil arthropods and 77.19% for soil nematodes. In addition, we used soil pH and soil organic C content as a proxy for soil chemical properties, soil bulk density as a proxy for soil physical properties and soil moisture (negatively correlated with soil temperature) as a proxy for soil micro-climatic conditions for an overall total of fourteen constituents.
We calculated ecosystem coupling9 for each exclosure treatment within each vegetation type (i.e., 2  5 treatment combinations in total) as an integrated measure of pairwise ecological interactions between ecosystem constituents representing ecological communities and the soil abiotic environment. These ecological interactions are defined by non-parametric Spearman rank correlation analyses between two constituents, excluding interactions involving two abiotic constituents (e.g., soil pH vs. soil moisture) and interactions between the first (PC1) and second (PC2) component of each community type, as these are orthogonal by definition. Interactions between abiotic constituents were excluded from the analyses because the focus of our study was on communities and how they interact with one another and their surrounding environment; therefore, including abiotic-abiotic interactions was not of interest here. Given that the effectiveness of our experimental design resulted in that no community composition data of aboveground-dwelling invertebrates was available for the “None” plots (all animals excluded), only thirteen instead of fourteen constituents were included in the ecosystem coupling calculations for this treatment. The complete absence of aboveground invertebrates represents the most extreme case of disturbance between aboveground animal communities and the rest of the ecosystem constituents. This may have resulted in a slight overestimation of ecosystem coupling for these plots.
	Average ecosystem coupling was calculated as follows:
Ecosystem coupling=
where Xi is the absolute Coupling was calculated value of the Spearman’s rho coefficient of the ith correlation for each treatment within each vegetation type (i.e., based on nine replicates each), considering and n is the number of pairwise comparisons considered (n = a total of 80; interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).	Coupling was calculated for each treatment within each vegetation type (i.e., based on nine replicates each), considering a total of 80 interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).
To establish whether constituents were significantly and positively coupled within treatments (i.e., the average of their correlation coefficients were greater than in a null model where correlation only happens by chance), we calculated one-tailed p-values based on permutation tests with 999 permutations.

We considered six ecosystem functions and process rates commonly used to assess ecosystem functioning (Meyer and others 2015; Manning and others 2018). Plant N content represents a measure of forage quality, while plant richness has been shown to stabilise biomass production, thus allowing the system to respond to changes in herbivory. Soil net N mineralisation, soil respiration, root biomass, and microbial biomass represent fluxes or stocks of energy. For all functions and processes higher values represent higher functioning (Manning and others 2018). All these variables were measured in the last year of the experiment (2013). We then quantified ecosystem multifunctionality using the multiple threshold approach (Byrnes and others 2014; Manning and others 2018), which considers the number of functions that are above a certain threshold, over a series of threshold values (typically 10-99%) that are defined based on the maximum value of each function. We weighted all our functions equally for these calculations (Manning and others 2018). The number of functions in a plot with values higher than a given threshold value for the respective function is summed up. The sum represents ecosystem multifunctionality for that plot. Given that choosing any particular threshold as a measure of ecosystem multifunctionality is arbitrary, we calculated the average of thresholds from 10-90% (in 10% intervals) as a more integrated representation of ecosystem multifunctionality.
We used Pearson correlations to explore the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and ecosystem multifunctionality by calculating the slopes of all relationships between ecosystem coupling and multifunctionality for all thresholds between 10 and 99%. We also related ecosystem coupling with the average of multifunctionality at thresholds between 30-80% as explained before and considered this correlation as a robust indication of the type of association between these two variables. In addition, we explored the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and individual ecosystem functions. The effects of exclosures and vegetation type on individual functions and multifunctionality were evaluated using linear mixed effects models ('lme' function of the nlme package), with exclosure and vegetation type as fixed effects and fence as a random factor. All statistical analyses and numerical calculations were done in R version 3.4.0 (R Core Team 2016).

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- Meyer ST, Koch C, Weisser WW. 2015. Towards a standardized Rapid Ecosystem Function Assessment (REFA). Trends Ecol Evol 30:390–7. http://www.sciencedirect.com/science/article/pii/S0169534715000968
- Norman R., Stucki JW. 1981. The determination of nitrate and nitrite in soil extracts by ultraviolet spectrophotometry. Soil Sci Soc Am J 45:347–53.
- Ochoa-Hueso R. 2016. Non-linear disruption of ecological interactions in response to nitrogen deposition. Ecology 87:2802–2814.
- Oostenbrink M. 1960. Estimating nematode populations by some selected methods. In: Sasser NJ, Jenkins WR, editors. Nematology. Chapel Hill, NC, USA: University of North Carolina Press. pp 85–101.
- R Core Team. 2016. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing
- Risch AC, Haynes AG, Busse MD, Filli F, Schütz M. 2013. The response of soil CO2 fluxes to progressively excluding vertebrate and invertebrate herbivores depends on ecosystem type. Ecosystems 16:1192–202.
- Risch AC, Schütz M, Vandegehuchte ML, Van Der Putten WH, Duyts H, Raschein U, Gwiazdowicz DJ, Busse MD, Page-Dumroese DS, Zimmermann S. 2015. Aboveground vertebrate and invertebrate herbivore impact on net N mineralization in subalpine grasslands. Ecology 96:3312–22.
- Schütz M, Risch AC, Achermann G, Thiel-Egenter C, Page-Dumroese DS, Jurgensen MF, Edwards PJ. 2006. Phosphorus translocation by red deer on a subalpine grassland in the Central European Alps. Ecosystems 9:624–633.
- Schütz M, Risch AC, Leuzinger E, Krüsi BO, Achermann G. 2003. Impact of herbivory by red deer (Cervus elaphus L.) on patterns and processes in subalpine grasslands in the Swiss National Park. For Ecol Manage 181:177–88.
- Vandegehuchte ML, van der Putten WH, Duyts H, Schütz M, Risch AC. 2017a. Aboveground mammal and invertebrate exclusions cause consistent changes in soil food webs of two subalpine grassland types, but mechanisms are system-speciﬁc. Oikos 126:212–23.
- Vandegehuchte ML, Raschein U, Schütz M, Gwiazdowicz DJ, Risch AC. 2015. Indirect short- and long-term effects of aboveground invertebrate and vertebrate herbivores on soil microarthropod communities. PLoS One 10:e0118679.
- Vandegehuchte ML, Schütz M, de Schaetzen F, Risch AC. 2017b. Mammal-induced trophic cascades in invertebrate food webs are modulated by grazing intensity in subalpine grassland. J Anim Ecol 86:1434–46.
- Vandegehuchte ML, Trivellone V, Schütz M, Firn J, de Schaetzen F, Risch AC. 2018. Mammalian herbivores affect leafhoppers associated with specific plant functional types at different timescales. Funct Ecol 32:545–55.
- Wirthner S, Frey B, Busse MD, Schütz M, Risch AC. 2011. Effects of wild boar (Sus scrofa L.) rooting on the bacterial community structure in mixed-hardwood forest soils in Switzerland. Eur J Soil Biol 47:296–302. http://dx.doi.org/10.1016/j.ejsobi.2011.07.003
- Zumsteg A, Luster J, Göransson H, Smittenberg RH, Brunner I, Bernasconi SM, Zeyer J, Frey B. 2012. Bacterial, Archaeal and Fungal Succession in the Forefield of a Receding Glacier. Microb Ecol 63:552–64. https://doi.org/10.1007/s00248-011-9991-8

ecosystem-coupling

display_name: ecosystem-coupling

ecosystem-functions

display_name: ecosystem-functions

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

exclusion

display_name: exclusion

grassland

display_name: grassland

grazing

display_name: grazing

invertebrates

display_name: invertebrates

subalpine

display_name: subalpine

swiss-national-park

display_name: swiss-national-park

vertebrates

display_name: vertebrates

en: Ecosystem coupling and multifunctionality - exclosure experiment


en: dem orthophoto uav drone findelengletscher gletscher glacier dsm orthoimage griesgletscher alps sankt-annafirn

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Griesgletscher on the 3rd of August 2015.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Griesgletscher on the 26th of September 2015.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Sankt Annafirn on the 15th of July 2015.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Findelengletscher on the 30th of August 2016.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Findelengletscher on the 21th of July 2015.

en: Bulk Data Access

en: Link to the S3 bucket where all the data resources can be downloaded in bulk

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Griesgletscher on the 15th of August 2015.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Griesgletscher on the 13th of September 2016.

en: This folder contains all UAV flights paths that we created for the Sankt Annafirn, the Findelengletscher and Griesgletscher. They have been created with the software eMotion2, that was developped for the eBee platform from Sensefly.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Findelengletscher on the 19th of April 2016.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Findelengletscher on the 15th of September 2015.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Findelengletscher on the 09th of April 2015.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Sankt Annafirn on the 15th of April 2015.

en: This .pdf contains an orthoimage for each dataset as well as a Table which displays the number of flights made, image acquired, number of GCP set and the total area covered.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Sankt Annafirn on the 28th of September 2015.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Findelengletscher on the 22th of September 2016.

en:
This .zip folder contains:

 - An orthoimage (oi) of 0.1m resolution

- A Digital Surface Model (dsm) of 0.1m resolution

- The Agisoft Photoscan automatic processing report

for the UAV field campaign conducted on the Findelengletscher on the 2nd of July 2016.

en:
 UAV-derived DSMs and orthoimages

Unmanned Aerial Vehicle (UAV) surveys were conducted between 2015 and 2016 on the Sankt Annafirn, Findelen- and Griesgletscher, situated in the Swiss Alps. Three surveys at the Sankt Annafirn allowed for a full glacier coverage, four surveys at Griesgletscher allowed an almost full glacier coverage and seven surveys at Findelengletscher allowed for a partial coverage of the glacier tongue (see individual datasets for exact extent). For each survey, a high resolution orthoimage and a Digital Surface Model (DSM) was created.

 UAV surveys:

Prior flight, Ground Control Points (GCPs) were deployed on the glacier surface and measured with a differential GPS (Trimble R7 or Leica GPS 1200). They allowed precise georeferencing of the UAV-derived datasets. UAV flight plans were planned with the software *eMotion 2* and a SenseFly eBee was used as surveying platform. The images were then processed with the software Agisoft Photoscan Pro 1.1.6 .

The location and dates of each survey can be found in the table together with the number of flights performed (Nflights), the number of acquired images (Nimages), the number of GCPs set (NGCPs) and the surveyed area.

A folder for each dataset is available (see folder name in table), which contains:

- An orthoimage *glacier_date_photoscan_oi_CH1903+_LV95_0.1m.tif*

- A Digital Surface Model *glacier_date_photoscan_dsm_CH1903+_LV95_0.1m.tif*

- The Agisoft Photoscan automatic processing report  *glacier_date_photoscan_report.pdf*

where:

- *glacier* is the name of the surveyed glacier

- *date* is the date of the UAV image acquisition

- *photoscan* is the name of the photogrammetric software

- *oi* or *dsm* the type of dataset

- *CH1903+_LV95* is the coordinate system and datum of the dataset

- *0.1m* is the resolution of the dataset in meter

- *.tif* is the extention of the dataset

Details about the UAV surveys, the image processing and the accuracy of the UAV-derived products can be found in this publication below.

Paper Citation:
 > _Gindraux et al. 2017. Accuracy Assessment of Digital Surface Models from Unmanned Aerial Vehicles’Imagery on Glaciers, Remote Sensing, 9, 186, 1-15, [doi: 10.3390/rs9020186](https://doi.org/10.3390/rs9020186)._

The folder UAV_flight_paths.zip contains all UAV flights performed on the Sankt Annafirn, Findelengletscher and Griesgletscher. The flights were planned with the software eMotion2 and have the .afp extention.

alps

display_name: alps

dem

display_name: dem

drone

display_name: drone

dsm

display_name: dsm

findelengletscher

display_name: findelengletscher

glacier

display_name: glacier

gletscher

display_name: gletscher

griesgletscher

display_name: griesgletscher

orthoimage

display_name: orthoimage

orthophoto

display_name: orthophoto

sankt-annafirn

display_name: sankt-annafirn

uav

display_name: uav

en: UAV-derived Digital Surface Models and orthoimages for three alpine glaciers


restoring-grassland-multifunctionality

title_for_slug: restoring-grassland-multifunctionality

en: soil-biodiversity bacteria mineralization biodiversity fungi grassland ecosystem-multifunctionality nematodes restoration plants

media_type: XLSX

en: Please note that C.sto and Corg have been corrected.

en: Please note that EMF, MF.below, MF.abio and Soil.hetero have been corrected.

en: Functional diversity above_belowground fauna

en:
Please cite this paper together with the citation for the datafile.

Resch, M. C., Schütz, M., Buchmann, N., Frey, B., Graf, U., van der Putten, W. H., Zimmermann, S., Risch, A. C. 2021. Evaluating long-term success in grassland restoration – an ecosystem multifunctionality approach. Ecological Applications 31, e02271.

 Study area

The study was conducted in the Canton of Zurich, Switzerland, in and around two nature reserves Eigental and Altläufe der Glatt (47°27’ to 47°29’ N, 8°37’ to 8°32’ E, 417 to 572 m a.s.l.). All studied grasslands were located with a radius of approximately 4 km. Average monthly temperatures range from 0.7 ± 2.0 °C (January) to 19.0 ± 1.5 °C (July), and monthly precipitation range from 60 ± 42 mm (January) to 118 ± 46 mm (July [maxima]; 1989-2017; MeteoSchweiz 2018). In our study, we focused on semi-dry and semi-wet oligo- to mesotrophic grasslands characterized by high plant species richness and groundwater fluctuations throughout the year (Delarze et al. 2015, see also Resch et al. 2019).

 Experimental design and sampling

A large-scale restoration experiment to expand and reconnect isolated remnants of species-rich grasslands was initiated in the nature reserve Eigental in 1990. Twenty hectares of adjacent intensive grasslands were chosen for restoration. In 1995, three restoration methods of increasing intervention intensities were implemented. The goal of all three methods was to lower the availability of soil nutrients and hence, facilitate ecosystem development towards the targeted nutrient-poor grasslands. These methods were: Harvest only (hay harvest twice a year), Topsoil (removal of the nutrient-rich topsoil), and Topsoil+Propagules (topsoil removal combined with the application of hay from target vegetation). Plant biomass harvest (once a year in late summer/early autumn) commenced in Topsoil and Topsoil+Propagules five years after the soils were removed and is still ongoing today. We measured restoration success by comparing the three restoration methods with intensively managed (Initial) and semi-natural grasslands (Target) 22 years after restoration. Initial grassland sites share the same agricultural history as the restored sites: mowing and subsequent fertilizing (manure) up to five times a year, as well as different tillage regimes (Resch et al. 2019). Target sites were the sites from which hay for seeding the Topsoil+Propagules sites was collected. Soil conditions (i.e., soil types, soil texture) were comparable to those found in the restored grasslands (Resch et al. 2019). Additionally, Target sites were selected to represent a variety of semi-natural grasslands, including semi-dry to semi-wet conditions. In Target grasslands, biomass is harvested once a year in late summer or early autumn. Eleven 5 m x 5 m (25 m2) plots were randomly established in each of the five treatments (in total 55 plots; for a detailed map see Neff et al. 2020). An additional 2 m x 2 m (4 m2) subplot was randomly established at least 2 m away from each 25 m2 plot for destructive sampling. Data sampling took place between June and September 2017.

Vegetation properties

All plant species were identified within the 25 m2 plots (nomenclature: Lauber and Wagner 1996) in mid-June 2017 (in total 250 species). Vegetation structure and plant biomass were assessed diagonally on a transect of 2 m x 10 cm within the 25 m2 plot in early July 2017. We measured the maximum and mean height of the vegetation at the start, middle and end of the transect and calculated the standard deviation of these measures to describe vegetation structural heterogeneity (Schuldt et al. 2019). Thereafter, biomass was clipped on the entire transect to 1 cm height, sorted into five functional groups (graminoids, forbs, legumes, litter, and woody species), dried at 60 °C for 48 h, and weighed (Meyer et al. 2015).

 Aboveground arthropods

Aboveground arthropods were sampled at two locations in each 25 m2 plot in early July 2017 (see also Neff et al. 2020). Briefly, two cylindrical baskets (50 cm diameter, 67 cm height; woven fabric) were thrown simultaneously from outside the plot into two opposite corners. A closable mosquito mesh sleeve was mounted to the top of the baskets and an integrated metal ring at the bottom was fixed to the ground with metal stakes to assure that insects could not escape. A suction sampler (Vortis, Burkhard Manufacturing Co. Ltd., Hertfordshire, England) was then inserted into one of the baskets through the opening of the sleeve and the plot was “vacuumed" twice for 105 seconds with a 30 seconds break. The collected animals were immediately transferred into 70% ethanol. Arthropods were sorted and assigned to 23 taxonomic groups. Holometabolic larvae were lumped into one category while hemimetabolic larvae were grouped separately from adults in the respective taxonomic rank. We used mean values of individuals per plot for total abundance. Aboveground arthropod richness was defined by the number of different taxa to lowest taxonomic level (in total 23 taxa). All taxa were assigned to one of five trophic levels: 1) primary producers, 2) primary consumers, 3) secondary consumers, 4) tertiary consumers, and 5) quaternary consumers.

 Belowground fauna

Sampling of all belowground fauna took place in mid-July 2017. Earthworms were sampled in two 30 cm x 30 cm x 20 cm soil monoliths at two opposite corners of the 25 m2 plot (opposite to aboveground arthropod sampling). The excavated soil monolith was broken by hand, all earthworms collected and immediately transferred in a 4% formaldehyde solution. Thereafter, earthworm individuals were identified to species level (in total 10 taxa; Christian and Zicsi 1999) and species assigned to three functional groups (Bouché 1977).
To assess soil arthropod communities, we randomly collected one undisturbed soil core (5 cm diameter, 12 cm depth) in each 4 m2 subplot with a slide hammer corer lined with a plastic sleeve (AMS Samplers, American Falls, Idaho, USA). Soil arthropods were extracted using Berlese-Tullgren funnels (3 mm mesh), starting the day of sampling and lasting 14 days. Individuals were stored in 70% ethanol. Soil arthropods were assigned to 41 taxonomic groups and 4 feeding types. Holometabolic and hemimetabolic larvae were treated as previously described for aboveground arthropods. Belowground arthropod richness refers to the 41 taxonomic groups.

For soil nematode sampling, we randomly collected eight soil cores of 2.2 cm diameter (Giddings Machine Company, Windsor, CO, USA) within each 4 m2 subplot to a depth of 12 cm. The eight cores were combined, gently homogenized, placed in coolers, kept at 4 °C and transported to the laboratory at NIOO in Wageningen (NL) within one week after collection. Free-living nematodes were extracted from 200 g of fresh soil using Oostenbrink elutriator (Oostenbrink 1960) and prepared for morphological identification and quantification as described by Resch et al. (2019). Nematodes were identified to family level (39 taxa) according to Bongers (1988), assigned to 17 functional groups, 5 feeding types and 5 colonizer-persister (C-P) classes (Yeates et al. 1993, Bongers 1990, Resch et al. 2019).

We randomly collected two more soil cores (2.2 cm diameter x 12 cm depth) within each 4 m2 subplot to determine soil microbial communities. Again, the soil cores were combined, homogenized, placed in coolers and transported to the laboratory at WSL in Birmensdorf (Switzerland) where the metagenomic DNA was extracted from 8 g sieved soil (2 mm) using the DNeasy PowerMax Soil Kit (Quiagen, Hilden, NRW, GER) according to the manufacturer`s instructions. PCR amplification of the V3-V4 region of the prokaryotic small-subunit (16S) and the ribosomal internal transcribed spacer region (ITS2) of eukaryotes was performed with 1 ng of template DNA utilizing PCR primers and conditions as previously described (Frey et al., 2016). PCRs were run in triplicates and pooled. The pooled amplicons were sent to the Genome Quebec Innovation Centre (Montreal, QC, Canada) for barcoding using the Fluidigm Access Array technology (Fluidigm) and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA). Quality filtering, clustering into operational taxonomic units (OTUs) and taxonomic assignment were performed as described by Frey et al. (2016) and Adamczyk et al. (2019). We used a customised pipeline largely based on UPARSE (Edgar 2013) implemented in USEARCH v. 9.2 (Edgar 2010). After discarding singletons of dereplicated sequences, clustering into OTUs with 97% sequence similarity was performed (Edgar 2013). Quality-filtered reads were mapped on the filtered set of centroid sequences. Taxonomic classification of prokaryotic and fungal sequences was conducted querying against most recent versions of SILVA (v.132, Quast et al. 2013) and UNITE (v.8, Nilsson et al. 2018). Only taxonomic assignments with confidence rankings equal or higher than 0.8 were accepted (assignments below 0.8 set to unclassified). Prokaryotic OTUs assigned to mitochondria or chloroplasts as well as eukaryotic OTUs assigned other than fungi were removed prior to data analysis. In addition, prokaryotic and fungal datasets were filtered to discard singletons and doubletons. Thereafter, OTU abundance matrices were rarefied to the lowest number of sequences per community, to normalize the total number of reads and achieve parity between samples (Prokaryota: 29,843 reads; Fungi: 26,690 reads). Finally, prokaryotic and fungal observed richness (number of OTUs) were estimated (Prokaryota: 14,010 OTUs; Fungi: 5,813 OTUs). For prokaryotes, we distinguished five and for fungi six functional types based on lowest taxonomic resolution (Nguyen et al. 2016, Tedersoo et al. 2014). Belowground taxon richness was defined by the total number of earthworm, arthropod, nematode, fungi, and prokaryote taxa assigned to lowest taxonomic level. Finally, all belowground taxa were assigned to the same five trophic levels as the aboveground arthropods.

 Soil chemical and physical properties, soil nitrogen mineralization

We randomly collected three 5 cm diameter x 12 cm depth soil samples in each 4 m2 subplot with a slide hammer corer (AMS Samplers, American Falls, Idaho, USA), pooled them and then made two subsamples. One was field-fresh and stored at 3 °C until analysis, the other was dried for 48 h at 60 °C and passed through a 4 mm mesh. From the dried sample, we measured soil pH potentiometrically in 0.01 M CaCl2 (soil:solution ratio=1:2; 30 minutes equilibration time). Total and organic carbon content were measured on fine-ground samples (≤ 0.5 mm) by dry combustion using a CN analyzer NC 2500 (CE Instruments, Wigan, United Kingdom). Inorganic carbon of samples with a pH > 6.5 was removed with acid vapor prior to analysis of organic carbon (Walthert et al. 2010). We calculated total soil carbon (C) storage after correcting its content for soil depth, stone content and density of fine earth (see below). Exchangeable cations were determined on another 5 g dry soil sample with 50 mL unbuffered 1 M NH4Cl solution (soil:solution ratio=1:10, end-over-end shaker for 1.5 hours) and measured by an ICP-OES (Optima 7300 DV, Perkin-Elmer, Waltham, Massachusetts, USA). Thereafter, cation exchange capacity (CEC) was calculated as the sum of exchangeable cations and protons (and expressed as mmolc per 1 kg soil) and used to describe nutrient retention capacity in our plots. Concentrations of exchangeable protons were calculated as the difference between total and Al-induced exchangeable acidity as determined by the KCl-method (Thomas 1982).

Ammonium (NH4+) and nitrate (NO3−) were extracted from a 20 g fresh subsample with 80 mL 1M KCl for 1.5 hours on an end-over-end shaker and filtered through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnemühle FineArt GmbH, Dassel, Germany). NH4+ concentrations were determined colorimetrically by automated flow injection analysis (FIAS 300, Perkin-Elmer, Waltham, Massachusetts, USA). NO3− concentrations were measured colorimetrically according to Norman and Stucki (1981). Potential soil net nitrogen (N) mineralization was assessed during an 8-week incubation period under controlled moisture (60% of field capacity), temperature (20 °C) and light conditions (dark) in the laboratory. We weighed duplicate samples of fresh soil equivalent to 8 g dry soil (24 h at 104 °C) into 50 mL Falcon tubes. Soil samples were extracted for NH4+ and NO3− at the beginning and after eight weeks as described above. Soil net N mineralization was calculated as the difference between the inorganic nitrogen (NH4+ and NO3−) before and after the incubation (Hart et al. 1994), corrected for the total incubation time and represented per day values expressed as mg N kg-1 soil d-1.

To assess soil physical properties, we randomly collected one undisturbed soil core per 4 m2 subplot (5 cm diameter, 12 cm depth) in a steel cylinder that fit into the slide hammer (AMS Samplers, American Falls, Idaho, USA). The cylinder was capped in the field to avoid disturbance. We then measured field capacity in the laboratory. For this purpose, the cylinder and soil therein were saturated in a water bath and drained on a sand/silt-bed with a suction corresponding to 60 cm hydrostatic head. The moist soil was dried at 105 °C to constant weight. Field capacity was calculated by dividing the mass of water by the total mass of wet soil contained at 60 cm hydrostatic head and used to describe water holding capacity. Thereafter, samples were passed through a 4 mm mesh. Fine-earth and skeleton fractions were weighed separately to assess bulk soil density (fine-earth plus skeleton), density of fine earth, and proportion of skeleton. Particle density was determined with the pycnometer method (Blake and Hartge 1986), and total porosity and proportion of fine pores were calculated (Danielson and Sutherland 1986). Clay, silt, and sand contents were quantified with the sediment method (Gee and Bauder 1986).

Surface and soil temperature (12 cm depth, water-resistant digital pocket thermometer; IP65, H-B Instrument, Trappe, Pennsylvania, USA) as well as volumetric soil moisture content (12 cm depth, time domain reflectometry; Field-Scout TDR 300, Spectrum Technologies, Aurora, Illinois, USA) were measured at five random locations within the 4 m2 subplots every month from June to September. We calculated the standard deviation of each temperature and moisture measure over four months to describe seasonal variations. Slope inclination was determined at plot-level via GPS measurements (GPS 1200, Leica Geosystem, Heerbrugg, Switzerland) and categorized into slope gradient classes according to FAO standards (1990). Thickness of the topsoil horizon (equivalent to Ah or Aa horizon) was determined at one soil monolith (30 x 30 x 30 cm3) per 4 m2 subplot in cm and rounded to next integer.

 References

Adamczyk, M., F. Hagedorn, S. Wipf, J. Donhauser, P. Vittoz, C. Rixen, A. Frossard, J. Theurillat, and B. Frey. 2019. The soil microbiome of GLORIA mountain summits in the Swiss Alps. Frontiers in Microbiology 10:1080-1101.

Blake, G.R., and K. H. Hartge. 1986. Particle Density. Pages 377-382 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Bongers, T. 1988. De nematoden van Nederland. Stichting Uitgeverij van de Koniklijke Nederlandse Natuurhistorische Verenigung (KNNV), Utrecht.

Bongers, T. 1990. The maturity index: an ecological measure of environmental disturbance based on nematode species composition. Oecologia 83:14-19.

Bouché, M. B. 1977. Strategies lombriciennes. Ecological Bulletins 25:122-132.

Christian, E., and A. Zicsi. 1999. Ein synoptischer Bestimmungsschlüssel der Regenwürmer Österreichs (Oligochaeta: Lumbricidae). Die Bodenkultur 50:121-131.

Danielson, R. E., and P. L. Sutherland. 1986. Porosity. Pages 443-461 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Delarze, R., Y. Gonseth, S. Eggenberg, and M. Vust. 2015. Lebensräume der Schweiz: Ökologie ‐ Gefährdung ‐ Kennarten, Ott Verlag, Bern.

Edgar, R. C. 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:2460–2461.

Edgar, R. C. 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods 10:996–998.

FAO. 1990. Guidelines for soil description, third ed. Land and Water Development Division at the Food and Agriculture Organization of the United Nations (FAO), Rome.

Frey, B., T. Rime, M. Phillips, B. Stierli, I. Hajdas, F. Widmer, and M. Hartmann. 2016. Microbial diversity in European alpine permafrost and active layers. FEMS Microbiology Ecology 92:fiw018.

Gee, G.W., and J. W. Bauder. 1986. Particle-size analysis. Pages 383-411 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Hart, S. C, J. M. Stark, E. A. Davidson, and M. K. Firestone. 1994. Nitrogen mineralization, immobilization, and nitrification. Pages 985-1016 in R. W. Weaver, S. Angle, P. Bottomley, D. Bezdicek, S. Smith, A. Tabatabai, and A. Wollum, editors. Methods of soil analysis: Part 2—Microbiological and biochemical properties. Soil Science Society of America (SSSA) Inc., Madison.

Lauber, K., Wagner, G., 1996. Flora Helvetica. Flora der Schweiz. Haupt Verlag, Bern.

MeteoSchweiz, 2018. Klimabulletin Jahr 2017. MeteoSchweiz, Zürich.

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bacteria

display_name: bacteria

biodiversity

display_name: biodiversity

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

fungi

display_name: fungi

grassland

display_name: grassland

mineralization

display_name: mineralization

nematodes

display_name: nematodes

plants

display_name: plants

restoration

display_name: restoration

soil-biodiversity

display_name: soil-biodiversity

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Restoring grassland multifunctionality