maintainer: Anita Risch

en: exclusion swiss-national-park ecosystem-functions invertebrates ecosystem-multifunctionality grazing vertebrates grassland ecosystem-coupling subalpine

en: Datafile

en:
ID: Plot ID composited of Fence & Treatment number (90 plots total)

Grassland: Name of the six subalpine grasslands where the exclosure setups were erected. In large grasslands (GRIM, MING, STAB), we established four hierarchical exclosure setups (two in short- and two in tall-grass vegetation), whereas in the small grasslands (BOTS, DADA, MARG) only one setup was erected per vegetation type. BOTS = Val dal Botsch, GRIM = Grimmels, MING = Val Mingér, STAB = Alp Stabelchod, DADA = Stabelchod da daint, MARG = Margunet

Fence: Number of exclosure setup (1 - 18). Note that uneven numbers (1,3,5,7,9,11,13,15,17) represent setups established on short-grass vegetation, even numbers (2,4,6,8,10,12,14,16,18) setups established in tall-grass vegetation
Vegetation	Vegetation type, 1 = short-grass vegetation, 2 = tall-grass vegetation; (9 replicate fences on each vegetation type)
Treatment	Progressive animal exclusion, numbers indicate which animals had access to the plot: 1 = Large/medium/small mammals, and invertebrates have access ("L/M/S/I"), 2 = Medium/small mammals, and invertebrates have access (“M/S/I”), 3 = Small mammals and invertebrates have access (“S/I”), 4 = Invertebrates have access (“I”), 5 = No animals have access (“None”)

Bulkden:	Fine fraction soil bulk density (g cm-3)

Soil.moist	: Soil moisture (%)

Soil.org.C: Rhizosphere soil C content (%)

pH: Soil pH

Nema.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil nematode community composition

Nema.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil nematode community composition

Micro.PC1: 	First principal component (PC1) of principal component analysis (PCA) of the soil microbial community composition

Micro.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil microbial community composition

Abv.Inv.PC1: First principal component (PC1) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Abv.Inv.PC2: Second principal component (PC2) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Arthr.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil arthropod community composition

Arthr.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil arthropod community composition

Plant.PC1: First principal component (PC1) of principal component analysis (PCA) of the plant community composition

Plant.PC2: Second principal component (PC2) of principal component analysis (PCA) of the plant community composition

Root.biomass: Root biomass (g m-2)

Micro.biomass	: Microbial biomass carbon (mg kg-1)

Plant.richness: Plant species richness

Plant.N: Plant tissue nitrogen content (%)

Soil.N.min: Soil net nitrogen mineralisation (kg ha-1 season)

Soil.resp: Soil carbon dioxide emissions (soil respiration; g m-2 h-1)

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:
 > Risch AC, Ochoa-Hueso R, van der Putten WH, Bump JK, Busse MD, Frey B, Gwiazdowicz DJ, Page-Dumroese DS, Vandegehuchte ML, Zimmermann S, Schütz M. Size-dependent loss of aboveground animals differentially affects grassland ecosystem coupling and functions. 2018. Nature Communications 9: 3684. [doi: 10.1038/s41467-018-06105-4](https://doi.org/10.1038/s41467-018-06105-4).

Please cite this paper together with the citation for the datafile.

#Methods
Study sites
The experimental exclosure setups were installed within the SNP (IUCN category Ia preserve; Dudley 2008), in south-eastern Switzerland. The park covers 172 km2 of forests and subalpine and alpine grasslands along with scattered rock outcrops and scree slopes. The entire area has been protected from human impact (no hunting, fishing, camping or off-trail hiking) since 1914. Large, fairly homogenous patches of short- and tall-grass vegetation, which originate from different historical management and grazing regimes, cover the park’s subalpine grasslands entirely. Short-grass vegetation developed in areas where cattle used to rest (nutrient input) prior to the park’s foundation (14th century to 1914) (Schütz and others 2003, 2006) and is dominated by lawn grass species such as Festuca rubra L., Briza media L. and Agrostis capillaris L. (Schütz and others 2003, 2006). Today, this vegetation type is intensively grazed by diverse vertebrate and invertebrate communities that inhabit the park and consume up to 60% of the available biomass (Risch and others 2013). Tall-grass vegetation developed where cattle formerly grazed, but did not rest, and is dominated by rather nutrient-poor tussocks of Carex sempervirens Vill. and Nardus stricta L. (Schütz and others 2003, 2006). This vegetation type receives considerably less grazing, with only roughly 20% of the biomass consumed (Risch and others 2013). Consequently, the two vegetation types together represent a long-term trajectory of changes in grazing regimes. Underlying bedrock of all grasslands is dolomite, which renders these grasslands rather poor in nutrients regardless of former and current land-use regimes.
Experimental design
To progressively exclude aboveground vertebrate and invertebrate animals, we established 18 size-selective exclosure setups (nine in short-grass, nine in tall-grass vegetation) distributed over six subalpine grasslands across the SNP (Risch and others 2013, 2015). Elevation differences of exclosure locations did not exceed 350 m (between 1975 and 2300 m a.s.l.). The exclosures were established immediately after snowmelt in spring 2009 and were left in place for five consecutive growing seasons (until end of 2013). They were, however, temporarily dismantled every fall (late October after first snowfall) to protect them from avalanches. They were re-established in the same location every spring immediately after snowmelt. Each size-selective exclosure setup consisted of five plots (2 x 3 m) that progressively excluded aboveground vertebrates and invertebrates from large to small. The plots are labelled according to the guilds that had access to them “L/M/S/I”, “M/S/I”, “S/I”, “I”, “None”; L = large mammals, M = medium mammals, S = small mammals, I = invertebrates, None = no animals had access. As we only had permission to have the experimental setup in place for five consecutive growing seasons, the experiment had to be completely dismantled in the late fall of 2013 and all material removed from the SNP.
Our exclosure design was aimed at excluding mammalian herbivores, but naturally also excluded the few medium and small mammalian predators, as well as the entire aboveground invertebrate food web. A total of 26 large to small mammal species can be found in the SNP, but large apex predators are missing (wolf, bear, lynx) . Reptiles, amphibians and birds are scarce to absent in the subalpine grasslands under study. Only two reptile species occur in the park and they are confined to rocky areas that warm up enough for them to survive. One frog species spawns in an isolated pond far from our grasslands. Only three bird species occasionally feed on the subalpine grasslands. Using game cameras (Moultrie 6MP Game Spy I-60 Infrared Digital Game Camera, Moultrie Feeders, Alabaster, AL, USA), we did observe that the medium- and small-sized mammals (marmot/hares and mice) were not afraid to enter the fences and feed on their designated plots. We never spotted reptiles, amphibians or birds on camera. We distinguished between 59 higher aboveground-dwelling invertebrate taxa that our size-selective exclosures excluded (see also methods for aboveground-dwelling invertebrates below).
The “L/M/S/I” plot (not fenced) was located at least 5 m from the 2.1 m tall and 7 x 9 m large main electrical fence that enclosed the other four plots. The bottom wire of this fence was mounted at 0.5 m height and was not electrified to enable safe access for medium and small mammals, while fencing out the large ones. Within each main fence, we randomly established four 2 x 3 m plots separated by 1-m wide walkways from one another and from the main fence line: 1) the “M/S/I” plots were unfenced, allowing access to all but the large mammals; 2) the “S/I” plots (10 x 10 cm electrical mesh fence) excluded all medium-sized mammals. Note that the bottom 10 cm of this fence remained non-electrified to enable safe access for small mammals; 3) the “I” plots (2 x 2 cm metal mesh fence) excluded all mammals. We double-folded the mesh at the bottom 50 cm to reduce the mesh size to smaller than 1 x 1 cm openings; and 4) the “None” plots were surrounded by a 1 m tall mosquito net (1.5 x 2 mm) to exclude all animals. The top of the plot was covered with a mosquito-meshed wooden frame mounted to the corner posts (roof). We treated these plots a few times with biocompatible insecticide (Clean kill original, Eco Belle GmbH, Waldshut-Tiengen, Germany) to remove insects that might have entered during data collection or that hatched from the soil, but amounts were negligible and did not impact soil moisture conditions within these plots.
To assess whether the design of the “None” exclosure (mesh and roof) affected the response variables within the plots and, therefore, influenced the results, we established an additional six “micro-climate control” exclosures (one in each of the six grasslands) (Risch and others 2013, 2015). These exclosures were built as the “None” exclosures but were open at the bottom (20 cm) of the 3-m side of the fence facing away from the prevailing wind direction to allow invertebrates to enter. A 20-cm high and 3-m long strip of metal mesh was used to block access to small mammals. Thus, this construction allowed a comparable micro-climate to the “None” plots, but also a comparable feeding pressure by invertebrates to the “I” plots. We compared various properties within these exclosures against one another to assess if our construction altered the conditions in the “None” plots. We showed that differences in plant (e.g., vegetation height, aboveground biomass) and soil properties (e.g., soil temperature, moisture) found between the “I” and the “None” treatments were not due to the construction of the “None” exclosure, but a function of animal exclusions, although the amount of UV light reaching the plant canopy was significantly reduced (Risch and others 2013).
Aboveground invertebrate sampling
Aboveground invertebrates were sampled with two different methods to capture both ground- and plant-dwelling organisms: 1) we randomly placed two pitfall traps (67 mm in diameter, covered with a roof) filled with 20% propylene glycol in one 1 x 1 m subplot of the 2 x 3 m treatment plots in spring 2013 (May) and emptied them every two weeks until late September 2013 (Vandegehuchte and others 2017b, 2018). A pitfall trap consisted of a plastic cylinder (13 cm depth, 6.75 cm diameter). Within each cylinder we placed a 100 ml plastic vial with outer diameter 6.70 cm and on top of the cylinder we placed a plastic funnel to guide the invertebrates into the vials. Each trap was cover with a cone-shaped and transparent plastic roof to protect the trap from rain (Vandegehuchte and others 2017b, 2018). Note that in the “None” plots only one trap was placed as control to check for effectiveness of the exclosure. 2) We vacuumed all invertebrates from a 60 x 60 cm area on another 1 x 1 m subplot with a suction sampler (Vortis, Burkhard manufacturing CO, Ltd., Rickmansworth, Hertfordshire, UK) every month from June to September 2013 (Vandegehuchte and others 2017b, 2018). For this purpose, we quickly placed a square plastic frame (60 x 60 x 40 cm) with a closable mosquito mesh sleeve attached to the top edge into the plot from the outside. The suction sample was then inserted into through the sleeve and operated for 45 s to collect the invertebrates (Vandegehuchte and others 2017b, 2018).
We sorted the ≈100 000 individuals collected with both methods by hand and identified each individual morphologically to the lowest taxonomic level feasible (59 taxa, including orders, suborders, subfamilies, families; phylum for Mollusca). These taxa belonged to the following feeding types: 19 herbivores, 16 detritivores, 9 predators, 8 mixed feeders, 5 omnivores and 2 non-classified feeders (or not feeding as adults) (Vandegehuchte and others 2017b). We summed the numbers from the two pitfall traps and the suction sampling over the course of the 2013 season to represent the aboveground invertebrate abundance and community composition of a plot. Note: we did not specifically attempt to catch flying invertebrates with e.g., sticky traps, thus a few flying insects may have been missed with our vacuum sampling approach.
Sampling of plant properties
The vascular plant species composition was assessed at peak biomass every summer (July) by estimating the frequency of occurrence of each species with the pin count method in each plot (Frank and McNaughton 1990). A total of 172 taxa occurred within our 90 plots and we calculated plant species richness for each plot separately. We used the 2013 data in this study. Plant quality was assessed every year in July and September; here we use plant quality at the end of the experiment (September 2013). Two 10 x 100 cm wide strips of vegetation per plot were clipped, combined, dried at 65°C, and ground (Pulverisette 16, Fritsch, Idar-Oberstein, Germany) to pass through a 0.5 mm sieve. Twenty randomly selected samples across all treatments were analysed for N (Leco TruSpec Analyser, Leco, St. Joseph, Michigan, USA) (Vandegehuchte and others 2015). Nitrogen concentrations of the other samples were then estimated from models established for the experiment and the entire SNP relating Fourier transform-near infrared reflectance (FT-NIR) spectra to the measured values of N using a multi-purpose FT-NIR spectrometer (Bruker Optics, Fällanden, Switzerland) (Vandegehuchte and others 2015). Root biomass was sampled every fall by collecting five 2.2 cm diameter x 10 cm deep soil samples (Giddings Machine Company, Windsor, CO, USA) per plot (450 samples year-1). The samples were dried at 30 °C and roots were sorted from the sample by hand. We sorted each sample for 1 h which allowed to retrieve over 90% of all roots present in the samples (Risch and others 2013). The roots were then dried at 65 °C for 48 and weighed to the nearest mg. We averaged the values per plot and used the 2013 data only in this study.
Sampling of edaphic communities
In 2009, 2010, and 2011 we collected three composited soil samples (5 cm diameter x 10 cm depth; AMS Samplers, American Falls, ID, USA) and assessed bacterial community structure using T-RFLP profiling (Liu and others 1997; Blackwood and others 2003; Hodel and others 2014). We detected a total of 89 operational taxonomic units (OTUs). These values are in accordance with other studies reporting OTU richness (Wirthner and others 2011; Zumsteg and others 2012; Meola and others 2014) using T-RFLP profiling, a method that detects the most abundant, and thus likely, the most relevant, taxa. We averaged the data over the three years of collections for our calculations. Microbial biomass carbon (MBC) was determined with the substrate-induced method (Anderson and Domsch 1978) every fall (September) between 2009 and 2013 by collecting three mineral soil samples (5 cm diameter × 10 cm mineral soil core, AMS Samplers, American Falls, ID, USA). The three samples were combined (90 samples for each sampling year), immediately put on ice, taken to the laboratory, passed through a 2-mm sieve and stored at 4°C. Again, we only used the 2013 data in this study.
Soil samples (5 cm diameter x 10 cm depth) to extract soil arthropods were collected in June, July, and August 2011 with a soil corer lined with a plastic sleeve to ensure an undisturbed sample (total of 270 samples). The plastic line core was immediately sealed on both ends using cling film and put into a cooler. All plots were sampled within three days and the extraction of arthropods started the evening of the sampling day using a high-gradient Tullgren funnel apparatus (Crossley and Blair 1991; Vandegehuchte and others 2015). Samples were kept in the extractor for four days and the soil arthropods were collected in 95% ethanol. All individuals were counted and each individual was identified morphologically to the lowest level feasible [76 taxa, including orders, suborders, subfamilies, families (Protura, Thysanoptera, Aphidina, Psylina, Coleoptera, Brachycera, Nematocera, Auchenorryncha, Heteroptera, Formicidae); sub-phylum for Myriapoda, for Acari and Collembola also including morpho-species). Note that we also included larval stages (nine of the 76 taxa) (Vandegehuchte and others 2015). All data were summed over the season. A detailed species list for mites and collembolans is published (Vandegehuchte and others 2017a) [https://doi.org/10.1371/journal.pone.0118679.s001]. Earthworms are rare in the SNP and therefore were not included. We collected eight random 2.2 cm diameter x 10 cm deep soil cores from each plot in September 2013 to determine the soil nematode community composition. The samples were mixed and the nematodes were extracted from 100 ml of fresh soil using Oostenbrink elutriators (Oostenbrink 1960). All nematodes in a 1 ml of the 10 ml extract were counted, a minimum of 150 individuals sample-1 were identified to genus or family level using (Bongers 1988), the numbers of all nematodes were extrapolated to the entire sample and expressed for a 100 g dry sample. In total we identified 63 genus or family levels (Vandegehuchte and others 2015). The list of all the nematodes found is published (Vandegehuchte and others 2015) [http://www.oikosjournal.org/appendix/oik-03341] or DOI: [doi: 10.1111/oik.03341].
We are aware that sampling soil microbes from 2009 to 2011 and soil arthropods in 2011 was not ideal, but we are positive that this does not bias the results. Most of the parameters measured in our experiment either already showed a treatment response after the first growing season (e.g., plant biomass) or did not respond over the entire time experiment (e.g., microbial biomass C). The microbial community composition (2009 – 2011) was highly influenced by inter-annual differences in temperature and precipitation, but did not differ between treatments or vegetation types (Hodel and others 2014). We therefore felt comfortable using the 2009 through 2011 data for describing the soil microbial community in our experimental treatments. Similarly, we are positive that our soil arthropod data are representative. We did assess soil arthropods in August 2012 and found no differences to the August 2011 data. However, we did not feel comfortable combining the 2011 June, July, August data with only August data for 2012 for our analyses.
Sampling of soil properties
We collected three soil samples (5 cm diameter x 10 cm depth) in each plot in September 2013 after removing the vegetation. First, we collected the top layer of mineral soil rich in organic matter, the surface organic layer or rhizosphere, typically 1 to 3 cm in depth with a soil corer (AMS Samples, American Falls, Idaho, USA). Second, we collected a 10 cm mineral soil core beneath this surface layer. The cores for each layer were composited, dried at 65 °C for 48 h and fine-ground to pass a 0.5 mm screen. We then analysed all samples for total C using a Leco TruSpec Analyser (Leco, St. Joseph, Michigan, USA). Mineral soil pH was measured potentiometrically in 1:2 soil:CaCl2 solution with an equilibration time of 30 min.
Soil net N mineralisation was assessed during the 2013 growing season (Risch and others 2015). For this purpose, we randomly collected a 5 cm diameter x10 cm deep soil sample with a soil corer (AMS Samples, American Falls, Idaho, USA) after clipping the vegetation in June 2013. After weighing and sieving (4 mm mesh) the soil, we extracted a 20 g subsample in 1 mol l-1 KCl for 1.5 h on an end-over-end shaker and thereafter filtered it through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnenmühle FineArt GmbH, Dassel, Germany). From these filtrates NO3- concentrations were measured colorimetrically (Norman and Stucki 1981) and NH4+with flow injection analysis (FIAS 300, Perkin Elmer, Waltham Massachusetts, USA) (Risch and others 2015). We dried the rest of the sample 105 °C to constant mass to determine fine,fraction bulk density. A second soil sample was collected within each plot in June 2013 with a corer lined with a 5 x 13 cm aluminium cylinder. The corer was driven 11.5 cm deep into the soil so that the top 1.5 cm of the cylinder remained empty. Into this space we placed a polyester bag (250 µm) filled an ion-exchanger resin to capture the incoming N. The bag was filled with a 1:1 mixture of acidic and alkaline exchanger resin (ion-exchanger I KA/ion exchanger IIIAA, Merck AG, Darmstadt, Germany). We then removed 1.5 cm soil at the bottom of the cylinder and placed a second resin exchanger bag into this space to capture the N leached from the soil column. To assure that the exchange resin was saturated with H+ and Cl- prior to filling the bags, the mixture was stirred with 1.2 ml l-1 HCl for 1 h and then rinsed with demineralized water until the electrical conductivity of the water reached 5 µm cm-1. The cylinder with the resin bags in place was reinserted into the soil with the top flush to the soil surface and incubated for three months. We recollected the cylinders in September 2013. Each resin bag and 20 g of sieved soil (4 mm mesh) from each cylinder were then separately extracted with KCl and NO3- and NH4+ concentrations were measured. Nitrate and NH4+ concentrations of all samples were then converted to a content basis by multiplying their values with fine fraction bulk density. Net N mineralisation was thereafter calculated as the difference between the N content of the samples collected at the end of the three-month incubation (including the N extracted from the bottom resin bag) and the N content at the beginning of the incubation (Risch and others 2015).
Soil CO2 emissions were measured every two weeks between 0900 and 1700 hrs from early May through late September 2013 with a PP-Systems SRC-1 soil respiration chamber (15 cm high, 10 cm diameter; closed circuit) attached to a PP-Systems EGM-4 infrared gas analyser (PP-Systems, Amesbury, MA, USA) on two locations per plot (Risch and others 2013). The chamber was placed on randomly placed, permanently installed PVC collars (10 cm diameter) driven 5 cm into the soil at the beginning of the study (Risch and others 2013). Freshly germinated plants growing within the collars were removed prior to each measurement to avoid measuring plant respiration or photosynthesis. The two measurements collected per plot and sampling date were averaged.
Soil moisture (with time domain reflectometry; Field-Scout TDR-100, Spectrum Technologies, Plainfield, Illionois, USA) and temperature (with a waterproof digital pocket thermometer; Barnstead International, Dubuque, Iowa, USA) were measured at five random locations per plot every two weeks during the growing seasons during the experiment for the 0 to 10 cm depth (Risch and others 2013, 2015). As soil moisture and soil temperature were highly negatively correlated (Risch and others 2013), we only used soil moisture for this study. We used plot-level averages of all values available to capture soil moisture variability during the five years of the experiment. The results remained unchanged when we only used soil moisture from the 2013 growing season.
Numeral calculations and statistical analyses
Ecosystem coupling. We conducted principal component analyses (PCAs; unscaled) at the complete dataset level using the abundances of each taxonomical entity to describe each of the five different communities used in this study: aboveground-dwelling invertebrates, vascular plants, soil microorganisms, soil arthropods and soil nematodes. We retained the first two components (PCA axis 1 and PCA axis 2) of each analysis as we found them to adequately represent the temporal and spatial variability of our 90 treatment plots in previous studies55,67. Together they explained a total of 71.70% of the variation for aboveground invertebrates, 44.36% for plants, 44.85% for soil microorganisms, 61.85% for soil arthropods and 77.19% for soil nematodes. In addition, we used soil pH and soil organic C content as a proxy for soil chemical properties, soil bulk density as a proxy for soil physical properties and soil moisture (negatively correlated with soil temperature) as a proxy for soil micro-climatic conditions for an overall total of fourteen constituents.
We calculated ecosystem coupling9 for each exclosure treatment within each vegetation type (i.e., 2  5 treatment combinations in total) as an integrated measure of pairwise ecological interactions between ecosystem constituents representing ecological communities and the soil abiotic environment. These ecological interactions are defined by non-parametric Spearman rank correlation analyses between two constituents, excluding interactions involving two abiotic constituents (e.g., soil pH vs. soil moisture) and interactions between the first (PC1) and second (PC2) component of each community type, as these are orthogonal by definition. Interactions between abiotic constituents were excluded from the analyses because the focus of our study was on communities and how they interact with one another and their surrounding environment; therefore, including abiotic-abiotic interactions was not of interest here. Given that the effectiveness of our experimental design resulted in that no community composition data of aboveground-dwelling invertebrates was available for the “None” plots (all animals excluded), only thirteen instead of fourteen constituents were included in the ecosystem coupling calculations for this treatment. The complete absence of aboveground invertebrates represents the most extreme case of disturbance between aboveground animal communities and the rest of the ecosystem constituents. This may have resulted in a slight overestimation of ecosystem coupling for these plots.
	Average ecosystem coupling was calculated as follows:
Ecosystem coupling=
where Xi is the absolute Coupling was calculated value of the Spearman’s rho coefficient of the ith correlation for each treatment within each vegetation type (i.e., based on nine replicates each), considering and n is the number of pairwise comparisons considered (n = a total of 80; interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).	Coupling was calculated for each treatment within each vegetation type (i.e., based on nine replicates each), considering a total of 80 interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).
To establish whether constituents were significantly and positively coupled within treatments (i.e., the average of their correlation coefficients were greater than in a null model where correlation only happens by chance), we calculated one-tailed p-values based on permutation tests with 999 permutations.

We considered six ecosystem functions and process rates commonly used to assess ecosystem functioning (Meyer and others 2015; Manning and others 2018). Plant N content represents a measure of forage quality, while plant richness has been shown to stabilise biomass production, thus allowing the system to respond to changes in herbivory. Soil net N mineralisation, soil respiration, root biomass, and microbial biomass represent fluxes or stocks of energy. For all functions and processes higher values represent higher functioning (Manning and others 2018). All these variables were measured in the last year of the experiment (2013). We then quantified ecosystem multifunctionality using the multiple threshold approach (Byrnes and others 2014; Manning and others 2018), which considers the number of functions that are above a certain threshold, over a series of threshold values (typically 10-99%) that are defined based on the maximum value of each function. We weighted all our functions equally for these calculations (Manning and others 2018). The number of functions in a plot with values higher than a given threshold value for the respective function is summed up. The sum represents ecosystem multifunctionality for that plot. Given that choosing any particular threshold as a measure of ecosystem multifunctionality is arbitrary, we calculated the average of thresholds from 10-90% (in 10% intervals) as a more integrated representation of ecosystem multifunctionality.
We used Pearson correlations to explore the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and ecosystem multifunctionality by calculating the slopes of all relationships between ecosystem coupling and multifunctionality for all thresholds between 10 and 99%. We also related ecosystem coupling with the average of multifunctionality at thresholds between 30-80% as explained before and considered this correlation as a robust indication of the type of association between these two variables. In addition, we explored the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and individual ecosystem functions. The effects of exclosures and vegetation type on individual functions and multifunctionality were evaluated using linear mixed effects models ('lme' function of the nlme package), with exclosure and vegetation type as fixed effects and fence as a random factor. All statistical analyses and numerical calculations were done in R version 3.4.0 (R Core Team 2016).

#References

- Anderson J, Domsch K. 1978. A physiological method for the quantitative measurement of microbial biomass in soil. Soil Biol Biochem 10:215–21.
- Blackwood CB, Marsh T, Kim S-H, Paul EA. 2003. Terminal Restriction Fragment Length Polymorphism Data Analysis for Quantitative Comparison of Microbial Communities. Appl Environ Microbiol 69:926–32. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143601/
- Bongers T. 1988. De nematoden von Nederland. Schoorl, The Netherlands: Pirola
- Byrnes JEK, Gamfeldt L, Isbell F, Lefcheck JS, Griffin JN, Hector A, Cardinale BJ, Hooper DU, Dee LE, Duffy JE. 2014. Investigating the relationship between biodiversity and ecosystem multifunctionality: Challenges and solutions. Methods Ecol Evol 5:111–24.
- Crossley DAJ, Blair JM. 1991. A high-efficiency low-technology Tulgren-type extractor for soil microarthopods. Agric Ecosyst Environ 34:187–92.
- Dudley N. 2008. Guidelines for applying protected area managment categories. Gland: IUCN
- Frank DA, McNaughton SJ. 1990. Aboveground biomass estimation with the canopy intercept method: A plant growth form caveat. Oikos 57:57–60.
- Haynes AG, Schütz M, Buchmann N, Page-Dumroese DS, Busse MD, Risch AC. 2014. Linkages between grazing history and herbivore exclusion on decomposition rates in mineral soils of subalpine grasslands. Plant Soil 374.
- Hodel M, Schütz M, Vandegehuchte ML, Frey B, Albrecht M, Busse MD, Risch AC. 2014. Does the aboveground herbivore assemblage influence soil bacterial community composition and richness in subalpine grasslands? Microb Ecol 68:584–95.
- Liu WT, Marsh TL, Cheng H, Forney LJ. 1997. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ Microbiol 63:4516–22. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC168770/
- Manning P, van der Plas F, Soliveres S, Allan E, Maestre FT, Mace G, Whittingham MJ, Fischer M. 2018. Redefining ecosystem multifunctionality. Nat Ecol Evol 2:427–36. https://doi.org/10.1038/s41559-017-0461-7
- Meola M, Lazzaro A, Zeyer J. 2014. Diversity, resistance and resilience of the bacterial communities at two alpine glacier forefields after a reciprocal soil transplantation. Environ Microbiol 16:1918–34. https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.12435
- Meyer ST, Koch C, Weisser WW. 2015. Towards a standardized Rapid Ecosystem Function Assessment (REFA). Trends Ecol Evol 30:390–7. http://www.sciencedirect.com/science/article/pii/S0169534715000968
- Norman R., Stucki JW. 1981. The determination of nitrate and nitrite in soil extracts by ultraviolet spectrophotometry. Soil Sci Soc Am J 45:347–53.
- Ochoa-Hueso R. 2016. Non-linear disruption of ecological interactions in response to nitrogen deposition. Ecology 87:2802–2814.
- Oostenbrink M. 1960. Estimating nematode populations by some selected methods. In: Sasser NJ, Jenkins WR, editors. Nematology. Chapel Hill, NC, USA: University of North Carolina Press. pp 85–101.
- R Core Team. 2016. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing
- Risch AC, Haynes AG, Busse MD, Filli F, Schütz M. 2013. The response of soil CO2 fluxes to progressively excluding vertebrate and invertebrate herbivores depends on ecosystem type. Ecosystems 16:1192–202.
- Risch AC, Schütz M, Vandegehuchte ML, Van Der Putten WH, Duyts H, Raschein U, Gwiazdowicz DJ, Busse MD, Page-Dumroese DS, Zimmermann S. 2015. Aboveground vertebrate and invertebrate herbivore impact on net N mineralization in subalpine grasslands. Ecology 96:3312–22.
- Schütz M, Risch AC, Achermann G, Thiel-Egenter C, Page-Dumroese DS, Jurgensen MF, Edwards PJ. 2006. Phosphorus translocation by red deer on a subalpine grassland in the Central European Alps. Ecosystems 9:624–633.
- Schütz M, Risch AC, Leuzinger E, Krüsi BO, Achermann G. 2003. Impact of herbivory by red deer (Cervus elaphus L.) on patterns and processes in subalpine grasslands in the Swiss National Park. For Ecol Manage 181:177–88.
- Vandegehuchte ML, van der Putten WH, Duyts H, Schütz M, Risch AC. 2017a. Aboveground mammal and invertebrate exclusions cause consistent changes in soil food webs of two subalpine grassland types, but mechanisms are system-speciﬁc. Oikos 126:212–23.
- Vandegehuchte ML, Raschein U, Schütz M, Gwiazdowicz DJ, Risch AC. 2015. Indirect short- and long-term effects of aboveground invertebrate and vertebrate herbivores on soil microarthropod communities. PLoS One 10:e0118679.
- Vandegehuchte ML, Schütz M, de Schaetzen F, Risch AC. 2017b. Mammal-induced trophic cascades in invertebrate food webs are modulated by grazing intensity in subalpine grassland. J Anim Ecol 86:1434–46.
- Vandegehuchte ML, Trivellone V, Schütz M, Firn J, de Schaetzen F, Risch AC. 2018. Mammalian herbivores affect leafhoppers associated with specific plant functional types at different timescales. Funct Ecol 32:545–55.
- Wirthner S, Frey B, Busse MD, Schütz M, Risch AC. 2011. Effects of wild boar (Sus scrofa L.) rooting on the bacterial community structure in mixed-hardwood forest soils in Switzerland. Eur J Soil Biol 47:296–302. http://dx.doi.org/10.1016/j.ejsobi.2011.07.003
- Zumsteg A, Luster J, Göransson H, Smittenberg RH, Brunner I, Bernasconi SM, Zeyer J, Frey B. 2012. Bacterial, Archaeal and Fungal Succession in the Forefield of a Receding Glacier. Microb Ecol 63:552–64. https://doi.org/10.1007/s00248-011-9991-8

ecosystem-coupling

display_name: ecosystem-coupling

ecosystem-functions

display_name: ecosystem-functions

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

exclusion

display_name: exclusion

grazing

display_name: grazing

invertebrates

display_name: invertebrates

subalpine

display_name: subalpine

swiss-national-park

display_name: swiss-national-park

vertebrates

display_name: vertebrates

education

en: Education and science

en: Ecosystem coupling and multifunctionality - exclosure experiment


maintainer: Matthias Haeni

en: long-term precipitation relative-humidity temperature wind-speed par meteorology climate-change swiss-forest-lab meteo

state: active

type: dataset

en: Field station data (BAF)

media_type: text/csv

en: Data file.

format: CSV

mimetype: text/csv

en: Site photo of the field station (BAF)

media_type: image/jpeg

en: Site photo of the field station from below (viewing upwards the slope).

format: SERVICE

mimetype: image/jpeg

en: Site aerial photo (Beatenberg)

en: Site aerial photo from both Beatenberg stations. Source: Google. 2016. Site aerial photo from both Beatenberg stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Data plot of the field station (BAF)

media_type: application/pdf

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

format: PDF

mimetype: application/pdf

en: Forest station data (BAB)

en: Data plot of the forest station (BAB)

en: Site photo of the forest station (BAB)

en: Site photo of the forest station from below (viewing upwards the slope).

en:
High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Beatenberg in Switzerland where one station is located within a natural coniferous forest (BAB) with Norway spruce (_Picea abies_; 190-210 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, BAF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Beatenberg is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the
Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

fr: Education, science

de: Bildung, Wissenschaft

it: Formazione e scienza

envidat

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

en: EnviDat: the Environmental Data Portal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: Beatenberg, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


maintainer: EnviDat Support

en: cmip5 air-temperature orographic-precipitation precipitation-amount climate-change

en: Download Server

en:
Predicting future climatic conditions at high spatial resolution is essential for many applications in science. Here we present data for monthly time series of precipitation and minimum and maximum temperature for four downscaled global circulation models. We used model output statistics in combination with mechanistic downscaling (the CHELSA algorithm) to calculate mean monthly maximum and minimum temperatures, as well as monthly precipitation sums at ~5km spatial resolution globally for the years 1850-2100. We validated the performance of the downscaling algorithm by comparing model output with observed climates for the years 1950-2069.

CHELSA_cmip5_ts is licensed under a Creative Commons Attribution 2.0 Generic (CC BY 2.0) license.

air-temperature

display_name: air-temperature

orographic-precipitation

display_name: orographic-precipitation

precipitation-amount

display_name: precipitation-amount

education

en: Education and science

en: High resolution monthly precipitation and temperature timeseries for the period 2006-2100


maintainer: Marco Moretti

en: bettergardens honeybees gardens city4bees urban-biodiversity resource-availability zurich pollinator-experiment beekeeping urban-ecosystems urbanization wild-bees

media_type: CSV

en:
Mean, standard deviation , minimum and maximum values of six functional traits. The data has the following structure:
- Bee_species: bee scientific name
- mean_trait: mean value
- sd_trait: standard deviation
_min_trait: minimum value
- max_trait: maximum value
- trait: the six traits used. ITD = Intertegular distance (mm); Pheno.duration = duration of the phenology; Pheno.start = start of the phenology; Pheno.end = end of the phenology; Rel.tongue.length= relative tongue length (mm); Daytime.activity = daily activity (h)
DistNichePart: functional dissimilarity in a multidimentional trait space defined by five traits between wild bee individuals of a wild bee species and the honeybee individuals. Values are at the species level.

media_type: .csv

en:
The data contains the following columns:

  SiteID: numeric, the identity of the garden where the bee was sampled.
  Taxonname: character, species name
  Lecty: numeric discreet, a mesure of specialisation degree. Levels: 1 = monolectic; 2=oliolectic; 3=polilectic
  Pheno.start= numeric discreet, the starting week of the flying activity.
  Pheno.end= numeric discreet, the ending week of the flying activity.
  Pheno.duration=numeric discreet, Pheno.end - Pheno.start
  Daytime.activity = numeric continuous, individual-level measure of the day hour when the bee was sampled
  ITD = numeric continuous, individual-level measure of the InterTegular Distance, a metric of mobility in mm.
  Rel.tongue.length =numeric continuous, individual-level measure of the relative tongue length (Tongue length / ITD), a metric of specilaisation degree, in mm.

en: Metadata for the predictors and response dataset

en: Metadata for the predictor and response dataset

en: Response and predictors used for the SEMs

en:
Cities are socio-ecological systems that filter and select species, thus establishing unique species assemblages and biotic interactions. Urban ecosystems can host richer wild bee communities than highly intensified agricultural areas, specifically in resource-rich urban green spaces such as allotment and family gardens. At the same time, urban beekeeping has boomed in many European cities, raising concerns that the fast addition of a large number of managed bees could deplete the existing floral resources, triggering competition between wild bees and honeybees. The data has been used to investigated the interplay between resource availability and the number of honeybees at local and landscape scales and how this relationship influences wild bee diversity.

This dataset contains the raw and processed data supporting the findings from the paper: "Low resource availability drives feeding niche partitioning between wild bees and honeybees in a European city".

The data contains:
1. Bee trait measurements at the species and individual-level of five functional traits.
2. The values of the feeding niche partitioning (functional dissimilarity to honeybees)
3. The predictors of resource availability and beekeeping intensity at local and landscape scales used in the modelling of the paper for the 23 experimental sites.

beekeeping

display_name: beekeeping

bettergardens

display_name: bettergardens

gardens

display_name: gardens

honeybees

display_name: honeybees

pollinator-experiment

display_name: pollinator-experiment

resource-availability

display_name: resource-availability

urban-biodiversity

display_name: urban-biodiversity

urban-ecosystems

display_name: urban-ecosystems

urbanization

display_name: urbanization

wild-bees

display_name: wild-bees

zurich

display_name: zurich

en: Niche partitioning between wild bees and honeybees


present-weather-sensor-klosters

maintainer: Maxime Hervo

title_for_slug: present-weather-sensor-klosters

en: weather precipitation raclets visibility sensor

en: Present Weather Sensor Klosters

media_type: mat

en: Present Weather sensor measuring visibility and weather type installed at Klosters.

en: A present weather sensor (Vaisala PWD22) was deployed in Klosters (LON: 9.880413, LAT: 46.869019) for weather observation, combining the functions of a forwardscatter visibility meter and a present weather sensor. Besides measuring ambient light, it detects the intensity as well as the amount of both liquid and solid precipitation. More information can be found in the [User's Manual](ftp://ftp.cmdl.noaa.gov/aerosol/doc/manuals/PWD22_Manual.pdf).

precipitation

display_name: precipitation

raclets

display_name: raclets

sensor

display_name: sensor

visibility

display_name: visibility

weather

display_name: weather


en: multilocus-genotypes drosophila-suzukii simple-sequence-repeats nuclear-microsatellite-markers switzerland trichopria-drosophilae biological-control population-genetics

en: Multilocus genotypes (biallelic coding for 21 nuclear microsatellites) of Trichopria drosophilae described in Gugerli et al. Agrarforschung Schweiz 2019. Missing data denoted as '999'. Population codes (PopNo) are the following: 1, Vezia; 3, Contone ; 5, Davesco; 6, Corteglia; 7, Mezzana; 8, Stabio; 9, Gordola; 10, Sementina; 12, Brusata di Novazzano; 14, Monteggio; 16, Giubiasco; 17, ComoBP; 18, VD; 19, Weil_D; 20, Dossenheim; 21, BioPlanet_Como. Coordinates (CoordCH_E, CoordCH_N) refer to the Swiss coordinates system CH1903.

en: Table comprising all pertinent information for multiplex PCRs of species-specific nuclear microsatellite makers for Trichopria drosophilae, comprising all 21 newly developed markers, details on full sequences with GenBank accession numbers, primer sequences (including labeling and pig-tails), and repeat motives. PCRs with respective annealing temperatures (AnnealingTemp) refer to Type-It Multiplex PCR Kit (Qiagen, Hilden, Germany).

en:
 Nuclear microsatellite markers and genotype data for _Trichopria ddrosophilae_

This data set comprises (i) the characteristics of a set of 21 species-specific nuclear microsatellites for PCR amplification in _Trichopria drosophilae_ (ii) and genotype data for samples collected in southern Switzerland (Canton of Ticino), with few reference samples from Canton of Vaud, southern Germany, and northern Italy (lab-reared population).
Markers were developed by Ecogenics GmbH, Balgach (Switzerland), using MiSeq Nano 2x250 v2 format (on a mix of 10 individuals). Multiplex PCR assays for multilocus genotyping were established by Ecological Genetics (WSL Birmensdorf), and population genetic analyses are found in Gugerli et al., Agrarforschung Schweiz 2019.

biological-control

display_name: biological-control

drosophila-suzukii

display_name: drosophila-suzukii

multilocus-genotypes

display_name: multilocus-genotypes

nuclear-microsatellite-markers

display_name: nuclear-microsatellite-markers

population-genetics

display_name: population-genetics

simple-sequence-repeats

display_name: simple-sequence-repeats

switzerland

display_name: switzerland

trichopria-drosophilae

display_name: trichopria-drosophilae

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Nuclear microsatellite markers for Trichopria drosophilae, parasitoid wasp on Drosophila suzukii


mountain-permafrost-hydrology

maintainer: Jeannette Noetzli

title_for_slug: mountain-permafrost-hydrology

en: ice-rich-permafrost mass-movements climate-change-impacts monitoring-techniques mountain-permafrost-hydrology permafrost-degradation

en: DORA Persistent URL

media_type: PDF

en: Synthesis report chapter of the Hydro-CH2018 project of the Federal Office for the Environment (FOEN)

en: This report was prepared as one of the synthesis report chapters of the Hydro-CH2018 project of the Federal Office for the Environment (FOEN). In earlier reports such as the CH2014-IMPACTS report (CH-Impacts 2014), the topic of mountain permafrost hydrology was not addressed. Here, we provide a baseline of the available knowledge of mountain permafrost in the Swiss Alps for future reference. We compile an overview of the current understanding of mountain permafrost in the Swiss Alps, its distribution and characteristics, observed and projected changes, and expected impacts on slope stability, infrastructure and hydrological aspects. We also briefly describe the measurement techniques and modelling approaches applied. The chapter closes with a summary of the most important open research questions. The literature cited mainly includes studies on mountain permafrost published in scientific journals and assessments of long-term observation data. We focus on permafrost hydrology interactions wherever information is available. However, systematic studies on permafrost hydrology in mountain areas are still limited.

climate-change-impacts

display_name: climate-change-impacts

ice-rich-permafrost

display_name: ice-rich-permafrost

mass-movements

display_name: mass-movements

monitoring-techniques

display_name: monitoring-techniques

mountain-permafrost-hydrology

display_name: mountain-permafrost-hydrology

permafrost-degradation

display_name: permafrost-degradation

education

en: Education and science

en: Mountain Permafrost Hydrology


maintainer: Maria Elvira Murazzi

en: nanoplastic forest-trees uptake polystyrene 13c-labelling

en: Nanoplastic in forests experiment

media_type: XLS

en:
Data embargoed until August 2022, and it will be provided upon request

-  excel file containing: two working sheets (leaf supply and root supply experiment) with a table with the values 13C-nanopolystyrene in the different tissues of the two forest tree species; graph showing the differences between controls and treatments of 13C-nanopolystyrene enrichment for each tissue of each forest tree species; ANOVA table showing significant values after having exposed the tree species to 13C-nanopolystyrene

format: XLS

en:
The fate of plastic in the environment is of global concern, because its production recently has
increased strongly and it accumulates in terrestrial and aquatic ecosystems. Although some knowledge on
its role in aquatic and terrestrial ecosystems was gained in the recent decade, hitherto very little is known
about the impact of micro and nanoplastics in forest ecosystems.

The aim of this pioneering project was to explore if nanoplastics are taken up by forest trees species
through leaves or roots. In greenhouse experiments, we exposed leaves or roots of seedling of two forest
trees species to solutions with highly 13C-labelled polystyrene nanoparticles (13C-nPS, 99 atom%) and
examined if they were incorporated in different above- and belowground tissues.

Treated part of the trees for both species showed significant 13C-enrichment, indicating that trees take up nanoparticles. However, the overall 13C signal strength in tissues that were not exposed to the 13C label remained low (Δδ13C<1‰) and was confined to a few seedlings, leaving it ambiguous whether nanoplastic transport occurs or not.

We acknowledge that the new method developed might be not sensitive enough to unequivocally detect
mechanisms of nanoplastic uptake and transport at environmentally realistic concentrations.

forest-trees

display_name: forest-trees

nanoplastic

display_name: nanoplastic

polystyrene

display_name: polystyrene

uptake

display_name: uptake

en: Nanoplastics in forests: Exploring the effects of nanoplastics on forest soils and tree physiology (NanoPlast)


maintainer: Michael Lehning

en: alpine3d snow alpine3d-simulations snow-hydrology alpine-precipitation snow-cover snow-distribution

en: ADS datasets and simulation inputs

media_type: application/zip

en: The file contains the ADS datasets of snow depth and input data used for simulations.

format: ZIP

mimetype: application/zip

en:
Dataset (Model input, snow distribution and validation) for the precipitation scaling paper, which should be cited along with the data set citation.

This data is useful for distributed hydrological modelling or other tasks that involve the study of snow distribution and precipitation in the high Alpine.

The format of the data is for Alpine3D (models.slf.ch) model runs but other models could be used, too.

Please cite:

_Vögeli, C., Lehning, M., Wever, N., Bavay M., 2016: Scaling Precipitation Input to Spatially Distributed Hydrological Models by Measured Snow Distribution., Front. Earth Sci. 4: 108. doi: 10.3389/feart.2016.00108._

Dataset is provided as a single zip file.
The archive contains two directories, the valuable distributed snow depth maps for the landscape Davos and the simulation input.
The archive also contains the file: "ReadMeMetadataDataSetPrecipitationScaling" which explains the data structure.

alpine-precipitation

display_name: alpine-precipitation

snow

display_name: snow

snow-cover

display_name: snow-cover

snow-distribution

display_name: snow-distribution

snow-hydrology

display_name: snow-hydrology

en: Precipitation Scaling Data Set (Vögeli et al., Frontiers)


causal-effect-of-mfoz

title_for_slug: causal-effect-of-mfoz

en: built-up-land-change propensity-score-matching selection-bias causality fujian-province the-major-function-oriented-zone

en: Data and Stata code for repeating the results

media_type: ZIP

en:
Data.xlsx is the variables of 952 townships
Table 1-2 NN(1-1)_noreplace_caliper(0.01).do is STATA code to produce the results in Table 1 and 2
Figure 5 Distribution of the propensity scores.R is R code rto produce Figure 5
Figure 5 Standard mean difference.R is R code rto produce Figure 5

en:
Title: Closer to causality: How effective is spatial planning in governing built-up land expansion in Fujian Province, China?

Research objective: The Major Function Oriented Zone (MFOZ), the first strategic spatial plan in China, is developed to achieve a coordinated regional development, through spatial regulation and zoning of development. The MFOZ he MFOZ divided land into four major function-oriented zones: The development-optimized zone, the development-prioritised zone, the development-restricted zone, and the development-prohibited zone. We used propensity score marching to evaluate the effect of the MFOZ on built-up land expansion in Fujian Province over three time intervals (2013–2015, 2013–2018 and 2013–2020).

Data: Data.xlsx contains the variables of 954 towns in Fujian Province.
Town_ID is the town unique ID;
County_ID is the county unique ID;
City_ID is the city unique ID;
MFOZ is the the development-prioritised zone and the development-restricted zone (The development-optimized zone and the development-prohibited zone are excluded);
Builtup_13_15 is the built-up land expansion from 2013 to 2015;
Builtup_13_18 is the built-up land expansion from 2013 to 2018;
Builtup_13_20 is the built-up land expansion from 2013 to 2020;
Dis2water is the Euclidean distance from the town to the nearest waterbody;
Slope is the the average slope within the town;
GDP is the average GDP in 2010 within the town;
Pop is the average population in 2010 within the town;
Road is the average population in 2010 within the town;
Dis2city is the Euclidean distance from the town to the nearest prefectural city centre;
Nei_Arable, Nei_Forest, and Nei_Built.up are the area of arable land, forest land, and built-up land neighbouring town i in 2010.

Method: we used the propensity score matching to compare the changes in the amount of built-up land in the towns of the development-prioritised zone with the matched towns of the development-restricted zone. Additionally, we used three evaluation intervals (2013–2015, 2013–2018 and 2013–2020) to evaluate temporal variation in the causal effect of the MFOZ on built-up land expansion.

built-up-land-change

display_name: built-up-land-change

causality

display_name: causality

fujian-province

display_name: fujian-province

propensity-score-matching

display_name: propensity-score-matching

selection-bias

display_name: selection-bias

the-major-function-oriented-zone

display_name: the-major-function-oriented-zone

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Causal effect of MFOZ


restoring-grassland-multifunctionality

title_for_slug: restoring-grassland-multifunctionality

en: ecosystem-multifunctionality nematodes mineralization bacteria grassland soil-biodiversity fungi biodiversity plants restoration

media_type: XLSX

en: Please note that C.sto and Corg have been corrected.

en: Please note that EMF, MF.below, MF.abio and Soil.hetero have been corrected.

en: Functional diversity above_belowground fauna

en:
Please cite this paper together with the citation for the datafile.

Resch, M. C., Schütz, M., Buchmann, N., Frey, B., Graf, U., van der Putten, W. H., Zimmermann, S., Risch, A. C. 2021. Evaluating long-term success in grassland restoration – an ecosystem multifunctionality approach. Ecological Applications 31, e02271.

 Study area

The study was conducted in the Canton of Zurich, Switzerland, in and around two nature reserves Eigental and Altläufe der Glatt (47°27’ to 47°29’ N, 8°37’ to 8°32’ E, 417 to 572 m a.s.l.). All studied grasslands were located with a radius of approximately 4 km. Average monthly temperatures range from 0.7 ± 2.0 °C (January) to 19.0 ± 1.5 °C (July), and monthly precipitation range from 60 ± 42 mm (January) to 118 ± 46 mm (July [maxima]; 1989-2017; MeteoSchweiz 2018). In our study, we focused on semi-dry and semi-wet oligo- to mesotrophic grasslands characterized by high plant species richness and groundwater fluctuations throughout the year (Delarze et al. 2015, see also Resch et al. 2019).

 Experimental design and sampling

A large-scale restoration experiment to expand and reconnect isolated remnants of species-rich grasslands was initiated in the nature reserve Eigental in 1990. Twenty hectares of adjacent intensive grasslands were chosen for restoration. In 1995, three restoration methods of increasing intervention intensities were implemented. The goal of all three methods was to lower the availability of soil nutrients and hence, facilitate ecosystem development towards the targeted nutrient-poor grasslands. These methods were: Harvest only (hay harvest twice a year), Topsoil (removal of the nutrient-rich topsoil), and Topsoil+Propagules (topsoil removal combined with the application of hay from target vegetation). Plant biomass harvest (once a year in late summer/early autumn) commenced in Topsoil and Topsoil+Propagules five years after the soils were removed and is still ongoing today. We measured restoration success by comparing the three restoration methods with intensively managed (Initial) and semi-natural grasslands (Target) 22 years after restoration. Initial grassland sites share the same agricultural history as the restored sites: mowing and subsequent fertilizing (manure) up to five times a year, as well as different tillage regimes (Resch et al. 2019). Target sites were the sites from which hay for seeding the Topsoil+Propagules sites was collected. Soil conditions (i.e., soil types, soil texture) were comparable to those found in the restored grasslands (Resch et al. 2019). Additionally, Target sites were selected to represent a variety of semi-natural grasslands, including semi-dry to semi-wet conditions. In Target grasslands, biomass is harvested once a year in late summer or early autumn. Eleven 5 m x 5 m (25 m2) plots were randomly established in each of the five treatments (in total 55 plots; for a detailed map see Neff et al. 2020). An additional 2 m x 2 m (4 m2) subplot was randomly established at least 2 m away from each 25 m2 plot for destructive sampling. Data sampling took place between June and September 2017.

Vegetation properties

All plant species were identified within the 25 m2 plots (nomenclature: Lauber and Wagner 1996) in mid-June 2017 (in total 250 species). Vegetation structure and plant biomass were assessed diagonally on a transect of 2 m x 10 cm within the 25 m2 plot in early July 2017. We measured the maximum and mean height of the vegetation at the start, middle and end of the transect and calculated the standard deviation of these measures to describe vegetation structural heterogeneity (Schuldt et al. 2019). Thereafter, biomass was clipped on the entire transect to 1 cm height, sorted into five functional groups (graminoids, forbs, legumes, litter, and woody species), dried at 60 °C for 48 h, and weighed (Meyer et al. 2015).

 Aboveground arthropods

Aboveground arthropods were sampled at two locations in each 25 m2 plot in early July 2017 (see also Neff et al. 2020). Briefly, two cylindrical baskets (50 cm diameter, 67 cm height; woven fabric) were thrown simultaneously from outside the plot into two opposite corners. A closable mosquito mesh sleeve was mounted to the top of the baskets and an integrated metal ring at the bottom was fixed to the ground with metal stakes to assure that insects could not escape. A suction sampler (Vortis, Burkhard Manufacturing Co. Ltd., Hertfordshire, England) was then inserted into one of the baskets through the opening of the sleeve and the plot was “vacuumed" twice for 105 seconds with a 30 seconds break. The collected animals were immediately transferred into 70% ethanol. Arthropods were sorted and assigned to 23 taxonomic groups. Holometabolic larvae were lumped into one category while hemimetabolic larvae were grouped separately from adults in the respective taxonomic rank. We used mean values of individuals per plot for total abundance. Aboveground arthropod richness was defined by the number of different taxa to lowest taxonomic level (in total 23 taxa). All taxa were assigned to one of five trophic levels: 1) primary producers, 2) primary consumers, 3) secondary consumers, 4) tertiary consumers, and 5) quaternary consumers.

 Belowground fauna

Sampling of all belowground fauna took place in mid-July 2017. Earthworms were sampled in two 30 cm x 30 cm x 20 cm soil monoliths at two opposite corners of the 25 m2 plot (opposite to aboveground arthropod sampling). The excavated soil monolith was broken by hand, all earthworms collected and immediately transferred in a 4% formaldehyde solution. Thereafter, earthworm individuals were identified to species level (in total 10 taxa; Christian and Zicsi 1999) and species assigned to three functional groups (Bouché 1977).
To assess soil arthropod communities, we randomly collected one undisturbed soil core (5 cm diameter, 12 cm depth) in each 4 m2 subplot with a slide hammer corer lined with a plastic sleeve (AMS Samplers, American Falls, Idaho, USA). Soil arthropods were extracted using Berlese-Tullgren funnels (3 mm mesh), starting the day of sampling and lasting 14 days. Individuals were stored in 70% ethanol. Soil arthropods were assigned to 41 taxonomic groups and 4 feeding types. Holometabolic and hemimetabolic larvae were treated as previously described for aboveground arthropods. Belowground arthropod richness refers to the 41 taxonomic groups.

For soil nematode sampling, we randomly collected eight soil cores of 2.2 cm diameter (Giddings Machine Company, Windsor, CO, USA) within each 4 m2 subplot to a depth of 12 cm. The eight cores were combined, gently homogenized, placed in coolers, kept at 4 °C and transported to the laboratory at NIOO in Wageningen (NL) within one week after collection. Free-living nematodes were extracted from 200 g of fresh soil using Oostenbrink elutriator (Oostenbrink 1960) and prepared for morphological identification and quantification as described by Resch et al. (2019). Nematodes were identified to family level (39 taxa) according to Bongers (1988), assigned to 17 functional groups, 5 feeding types and 5 colonizer-persister (C-P) classes (Yeates et al. 1993, Bongers 1990, Resch et al. 2019).

We randomly collected two more soil cores (2.2 cm diameter x 12 cm depth) within each 4 m2 subplot to determine soil microbial communities. Again, the soil cores were combined, homogenized, placed in coolers and transported to the laboratory at WSL in Birmensdorf (Switzerland) where the metagenomic DNA was extracted from 8 g sieved soil (2 mm) using the DNeasy PowerMax Soil Kit (Quiagen, Hilden, NRW, GER) according to the manufacturer`s instructions. PCR amplification of the V3-V4 region of the prokaryotic small-subunit (16S) and the ribosomal internal transcribed spacer region (ITS2) of eukaryotes was performed with 1 ng of template DNA utilizing PCR primers and conditions as previously described (Frey et al., 2016). PCRs were run in triplicates and pooled. The pooled amplicons were sent to the Genome Quebec Innovation Centre (Montreal, QC, Canada) for barcoding using the Fluidigm Access Array technology (Fluidigm) and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA). Quality filtering, clustering into operational taxonomic units (OTUs) and taxonomic assignment were performed as described by Frey et al. (2016) and Adamczyk et al. (2019). We used a customised pipeline largely based on UPARSE (Edgar 2013) implemented in USEARCH v. 9.2 (Edgar 2010). After discarding singletons of dereplicated sequences, clustering into OTUs with 97% sequence similarity was performed (Edgar 2013). Quality-filtered reads were mapped on the filtered set of centroid sequences. Taxonomic classification of prokaryotic and fungal sequences was conducted querying against most recent versions of SILVA (v.132, Quast et al. 2013) and UNITE (v.8, Nilsson et al. 2018). Only taxonomic assignments with confidence rankings equal or higher than 0.8 were accepted (assignments below 0.8 set to unclassified). Prokaryotic OTUs assigned to mitochondria or chloroplasts as well as eukaryotic OTUs assigned other than fungi were removed prior to data analysis. In addition, prokaryotic and fungal datasets were filtered to discard singletons and doubletons. Thereafter, OTU abundance matrices were rarefied to the lowest number of sequences per community, to normalize the total number of reads and achieve parity between samples (Prokaryota: 29,843 reads; Fungi: 26,690 reads). Finally, prokaryotic and fungal observed richness (number of OTUs) were estimated (Prokaryota: 14,010 OTUs; Fungi: 5,813 OTUs). For prokaryotes, we distinguished five and for fungi six functional types based on lowest taxonomic resolution (Nguyen et al. 2016, Tedersoo et al. 2014). Belowground taxon richness was defined by the total number of earthworm, arthropod, nematode, fungi, and prokaryote taxa assigned to lowest taxonomic level. Finally, all belowground taxa were assigned to the same five trophic levels as the aboveground arthropods.

 Soil chemical and physical properties, soil nitrogen mineralization

We randomly collected three 5 cm diameter x 12 cm depth soil samples in each 4 m2 subplot with a slide hammer corer (AMS Samplers, American Falls, Idaho, USA), pooled them and then made two subsamples. One was field-fresh and stored at 3 °C until analysis, the other was dried for 48 h at 60 °C and passed through a 4 mm mesh. From the dried sample, we measured soil pH potentiometrically in 0.01 M CaCl2 (soil:solution ratio=1:2; 30 minutes equilibration time). Total and organic carbon content were measured on fine-ground samples (≤ 0.5 mm) by dry combustion using a CN analyzer NC 2500 (CE Instruments, Wigan, United Kingdom). Inorganic carbon of samples with a pH > 6.5 was removed with acid vapor prior to analysis of organic carbon (Walthert et al. 2010). We calculated total soil carbon (C) storage after correcting its content for soil depth, stone content and density of fine earth (see below). Exchangeable cations were determined on another 5 g dry soil sample with 50 mL unbuffered 1 M NH4Cl solution (soil:solution ratio=1:10, end-over-end shaker for 1.5 hours) and measured by an ICP-OES (Optima 7300 DV, Perkin-Elmer, Waltham, Massachusetts, USA). Thereafter, cation exchange capacity (CEC) was calculated as the sum of exchangeable cations and protons (and expressed as mmolc per 1 kg soil) and used to describe nutrient retention capacity in our plots. Concentrations of exchangeable protons were calculated as the difference between total and Al-induced exchangeable acidity as determined by the KCl-method (Thomas 1982).

Ammonium (NH4+) and nitrate (NO3−) were extracted from a 20 g fresh subsample with 80 mL 1M KCl for 1.5 hours on an end-over-end shaker and filtered through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnemühle FineArt GmbH, Dassel, Germany). NH4+ concentrations were determined colorimetrically by automated flow injection analysis (FIAS 300, Perkin-Elmer, Waltham, Massachusetts, USA). NO3− concentrations were measured colorimetrically according to Norman and Stucki (1981). Potential soil net nitrogen (N) mineralization was assessed during an 8-week incubation period under controlled moisture (60% of field capacity), temperature (20 °C) and light conditions (dark) in the laboratory. We weighed duplicate samples of fresh soil equivalent to 8 g dry soil (24 h at 104 °C) into 50 mL Falcon tubes. Soil samples were extracted for NH4+ and NO3− at the beginning and after eight weeks as described above. Soil net N mineralization was calculated as the difference between the inorganic nitrogen (NH4+ and NO3−) before and after the incubation (Hart et al. 1994), corrected for the total incubation time and represented per day values expressed as mg N kg-1 soil d-1.

To assess soil physical properties, we randomly collected one undisturbed soil core per 4 m2 subplot (5 cm diameter, 12 cm depth) in a steel cylinder that fit into the slide hammer (AMS Samplers, American Falls, Idaho, USA). The cylinder was capped in the field to avoid disturbance. We then measured field capacity in the laboratory. For this purpose, the cylinder and soil therein were saturated in a water bath and drained on a sand/silt-bed with a suction corresponding to 60 cm hydrostatic head. The moist soil was dried at 105 °C to constant weight. Field capacity was calculated by dividing the mass of water by the total mass of wet soil contained at 60 cm hydrostatic head and used to describe water holding capacity. Thereafter, samples were passed through a 4 mm mesh. Fine-earth and skeleton fractions were weighed separately to assess bulk soil density (fine-earth plus skeleton), density of fine earth, and proportion of skeleton. Particle density was determined with the pycnometer method (Blake and Hartge 1986), and total porosity and proportion of fine pores were calculated (Danielson and Sutherland 1986). Clay, silt, and sand contents were quantified with the sediment method (Gee and Bauder 1986).

Surface and soil temperature (12 cm depth, water-resistant digital pocket thermometer; IP65, H-B Instrument, Trappe, Pennsylvania, USA) as well as volumetric soil moisture content (12 cm depth, time domain reflectometry; Field-Scout TDR 300, Spectrum Technologies, Aurora, Illinois, USA) were measured at five random locations within the 4 m2 subplots every month from June to September. We calculated the standard deviation of each temperature and moisture measure over four months to describe seasonal variations. Slope inclination was determined at plot-level via GPS measurements (GPS 1200, Leica Geosystem, Heerbrugg, Switzerland) and categorized into slope gradient classes according to FAO standards (1990). Thickness of the topsoil horizon (equivalent to Ah or Aa horizon) was determined at one soil monolith (30 x 30 x 30 cm3) per 4 m2 subplot in cm and rounded to next integer.

 References

Adamczyk, M., F. Hagedorn, S. Wipf, J. Donhauser, P. Vittoz, C. Rixen, A. Frossard, J. Theurillat, and B. Frey. 2019. The soil microbiome of GLORIA mountain summits in the Swiss Alps. Frontiers in Microbiology 10:1080-1101.

Blake, G.R., and K. H. Hartge. 1986. Particle Density. Pages 377-382 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Bongers, T. 1988. De nematoden van Nederland. Stichting Uitgeverij van de Koniklijke Nederlandse Natuurhistorische Verenigung (KNNV), Utrecht.

Bongers, T. 1990. The maturity index: an ecological measure of environmental disturbance based on nematode species composition. Oecologia 83:14-19.

Bouché, M. B. 1977. Strategies lombriciennes. Ecological Bulletins 25:122-132.

Christian, E., and A. Zicsi. 1999. Ein synoptischer Bestimmungsschlüssel der Regenwürmer Österreichs (Oligochaeta: Lumbricidae). Die Bodenkultur 50:121-131.

Danielson, R. E., and P. L. Sutherland. 1986. Porosity. Pages 443-461 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Delarze, R., Y. Gonseth, S. Eggenberg, and M. Vust. 2015. Lebensräume der Schweiz: Ökologie ‐ Gefährdung ‐ Kennarten, Ott Verlag, Bern.

Edgar, R. C. 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:2460–2461.

Edgar, R. C. 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods 10:996–998.

FAO. 1990. Guidelines for soil description, third ed. Land and Water Development Division at the Food and Agriculture Organization of the United Nations (FAO), Rome.

Frey, B., T. Rime, M. Phillips, B. Stierli, I. Hajdas, F. Widmer, and M. Hartmann. 2016. Microbial diversity in European alpine permafrost and active layers. FEMS Microbiology Ecology 92:fiw018.

Gee, G.W., and J. W. Bauder. 1986. Particle-size analysis. Pages 383-411 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Hart, S. C, J. M. Stark, E. A. Davidson, and M. K. Firestone. 1994. Nitrogen mineralization, immobilization, and nitrification. Pages 985-1016 in R. W. Weaver, S. Angle, P. Bottomley, D. Bezdicek, S. Smith, A. Tabatabai, and A. Wollum, editors. Methods of soil analysis: Part 2—Microbiological and biochemical properties. Soil Science Society of America (SSSA) Inc., Madison.

Lauber, K., Wagner, G., 1996. Flora Helvetica. Flora der Schweiz. Haupt Verlag, Bern.

MeteoSchweiz, 2018. Klimabulletin Jahr 2017. MeteoSchweiz, Zürich.

Meyer, S. T., C. Koch, and W. W. Weisser. 2015. Towards a standardized rapid ecosystem function assessment (REFA). Trends in Ecology and Evolution 30:390-397.

Neff, F., M. C. Resch, A. Marty, J. Rolley, M. Schütz, A. C. Risch, and M. M. Gossner. 2020. Long-term restoration success of insect herbivore communities in semi-natural grasslands – a functional approach. Ecological Applications 0:e02133

Nguyen, N.H., Z. W. Song, S. T. Bates, S. Branco, L. Tedersoo, J. Menke, J. S. Schilling, and P. G. Kennedy. 2016. FUNGuild: an open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology 20:241–248.

Nilsson, R. H., K.-H. Larsson, A. F. S. Taylor, J. Bengtsson-Palme, T. S. Jeppesen, D. Schigel, P. G. Kennedy, K. Picard, F. O. Glöckner, L. Tedersoo, I. Saar, U. Kõljalg, and K. Abarenkov. 2018. The UNITE database for molecular identification of fungi: handling dark taxa and parallel taxonomic classifications. Nucleic Acids Research 47:259–264.

Norman, R. J., and J. W. Stucki. 1981. The Determination of Nitrate and Nitrite in Soil Extracts by Ultraviolet Spectrophotometry. Soil Science Society of America Journal 45:347-353.

Oostenbrink, M. 1960. Estimating nematode populations by some selected methods. Pages 81-101 in J. J. Sasser, and W. R. Jenkins, editors. Nematology. Univ. of North Carolina Press, Chapel Hill.

Quast, C., E. Pruesse, P. Yilmaz, J. Gerken, T. Schweer, P. Yarza, J. Peplies, and F. O. Glöckner. 2013. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Research 41:590-596.

Resch, M. C., M. Schütz, U. Graf, R. Wagenaar, W.H. van der Putten, and A. C. Risch. 2019. Does topsoil removal in grassland restoration benefit both soil nematode and plant communities? Journal of Applied Ecology 56:1782-1793.

Schuldt, A., A. Ebeling, M. Kunz, M. Staab, C. Guimarães-Steinicke, D. Bachmann, N. Buchmann, W. Durka, A. Fichtner, F. Fornoff, W. Härdtle, L. R. Hertzog, A-N. Klein, C. Roscher, J. Schaller, von G. Oheimb, A. Weigelt, W. Weisser, C.Wirth, J. Zhang, H. Bruelheide, and N. Eisenhauer. 2019. Multiple plant diversity components drive consumer communities across ecosystems. Nature Communications 10:1460.

Tedersoo, L., M. Bahram, S. Põlme, U. Kõljalg, N. S. Yorou, R. Wijesundera, L. Villarreal Ruiz, A. M. Vasco-Palacios, P. Q. Thu, A. Suija, M. E. Smith, C. Sharp, E. Saluveer, A. Saitta, M. Rosas, T. Riit, D. Ratkowsky, K. Pritsch, K. Põldmaa, M. Piepenbring, C. Phosri, M. Peterson, K. Parts, K. Pärtel, E. Otsing, E. Nouhra, A. L. Njouonkou, R. H. Nilsson, L. N. Morgado, J. Mayor, T. W. May, L. Majuakim, D. J. Lodge, S. See Lee, K.-H. Larsson, P. Kohout, K. Hosaka, I. Hiiesalu, T. W. Henkel, H. Harend, L.-D. Guo, A. Greslebin, G. Grelet, J. Geml, G. Gates, W. Dunstan, C. Dunk, R. Frenkhan, L. Dearnaley, A. De Kesel, T. Dang, X. Chen, F. Buegger, F. Q. Brearley, G. Bonito, S. Anslan, S. Abell, and K. Abarenkov. 2014. Global diversity and geography of soil fungi. Science 346:1256688.

Thomas, G.W. 1982. Exchangeable cations. Pages 159-165 in A. L. Page, R. H. Miller, and D. R. Keeney, editors. Methods of Soil Analysis: Part 2—Chemical and microbiological properties. Soil Science Society of America (SSSA) Inc., Madison.

Walthert, L., U. Graf, A. Kammer, J. Luster, D. Pezzotta, S. Zimmermann, and F. Hagedorn. 2010. Determination of organic and inorganic carbon, δ13C, and nitrogen in soils containing carbonates after acid fumigation with HCl. Journal of Plant Nutrition and Soil Sciences 173:207-216.

Yeates, G. W., T. Bongers, R. G. M. de Goede, D. W. Freckman, and S. S. Georgieva. 1993. Feeding habits in soil nematode families and genera – an outline for soil ecologists. Journal of Nematology 25:315-331.

bacteria

display_name: bacteria

biodiversity

display_name: biodiversity

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

fungi

display_name: fungi

grassland

display_name: grassland

mineralization

display_name: mineralization

nematodes

display_name: nematodes

plants

display_name: plants

restoration

display_name: restoration

soil-biodiversity

display_name: soil-biodiversity

education

en: Education and science

en: Restoring grassland multifunctionality


radiosondes

maintainer: Jörg Wieder

title_for_slug: radiosondes

en: relative-humidity pressure holoballoon temperature wind-direction radiosonde wind raclets

en: Radiosond Sounding Files

en:
The "soundings" radiosond data contains information on ambient pressure (mbar), temperature (C), relative humidity (%), wind speed (m s‾ ¹) and wind direction (true deg), and are ordered with respect to the height above ground (m AGL) with a step size of 20 m. The first line of each file idicates the geographic location and the start time (in UTC) of the ascent. If the wind speed was zero, no wind direction is given.
The zip files contain ascents on the following days:
February 10 (morning and afternoon),
February 11 (morning),
February 16 (afternoon),
February 22 (morning),
March 1 (morning),
March 5 (morning),
March 11 (morning),
March 12 (morning),
March 13 (morning),
March 14 (morning),
March 17 (afternoon), and
March 18.
The data was managed with Windsond Receiver Application Demo Version 2.92, (C) Sparv Embedded AB 2016.

en: Radiosond Template Files

en:
The "template" files contain standard parameters, i.e. ambient pressure (mbar and Pa), temperature (°C), relative humidity (%), wind speed (m s‾ ¹) and wind direction (true deg), as well as additional information, e.g. time (UTC and local time), dew point (°C), mixing ration (g/kg), density (kg m³), altitude (m MSL), height above ground (ft and m AGL), and geographic coordinates (longitude, latitude). If the wind speed was zero, no wind direction is given.
The zip files contain ascents on the following days:
February 10 (morning and afternoon),
February 11 (morning),
February 16 (afternoon),
February 22 (morning),
March 1 (morning),
March 5 (morning),
March 11 (morning),
March 12 (morning),
March 13 (morning),
March 14 (morning),
March 17 (afternoon), and
March 18.
The data was managed with Windsond Receiver Application Demo Version 2.92, (C) Sparv Embedded AB 2016.

en: Radiosondes (Windsond, Sparv Embedded AB) were started in Davos Wolfgang (LON: 9.853594, LAT: 46.835577) to report height profiles of pressure, relative humidity and temperature at specific days. In addition to regular launches of radiosondes, sondes were attached to [HoloBalloon](https://www.envidat.ch/group/clouds-in-situ-raclets) to report the ambient conditions of the in-situ measurements. Further profiles of meteorological measures were recorded at [HoloGondel](https://www.envidat.ch/group/clouds-in-situ-raclets) which was installed at the gondola moving between Gotschnaboden and Gotschnagrat at 2285 m a.s.l.

holoballoon

display_name: holoballoon

pressure

display_name: pressure

raclets

display_name: raclets

radiosonde

display_name: radiosonde

relative-humidity

display_name: relative-humidity

temperature

display_name: temperature

wind

display_name: wind

wind-direction

display_name: wind-direction

en: Radiosondes


en: terrestrial-laser-scanning drifting-snow mass-flux meteorological-data antarctica snowmicropen

en: Dataset

media_type: Link

en: Links to the dataset

en: README

media_type: text/plain

en: Describes the dataset in detail

format: TXT

mimetype: text/plain

en:
This dataset contains the data acquired during the expedition to Princess Elisabeth Antarctica Station in December 2016 and January 2017. The dataset consits of meterorological data, drifting snow mass flux data, SnowMicroPen data and Terrestrial Laser Scanning data.

Please refer to the README for more information about the data.

This dataset is the basis of the following publication:

Sommer, C. G., Wever, N., Fierz, C., and Lehning, M.: Wind-packing of snow in Antarctica, The Cryosphere Discuss., https://doi.org/10.5194/tc-2018-36, in review, 2018.

antarctica

display_name: antarctica

drifting-snow

display_name: drifting-snow

mass-flux

display_name: mass-flux

meteorological-data

display_name: meteorological-data

snowmicropen

display_name: snowmicropen

terrestrial-laser-scanning

display_name: terrestrial-laser-scanning

envidat

en: EnviDat: the Environmental Data Portal

en: Expedition to Princess Elisabeth Antarctica Station, 2016/2017


en: snow-instability failure-layer rutschblock snow-profiles snowpack-simulation snowpack snow-stratigraphy

en: Simulated snow profiles

en: Simulated snow stratigraphy in form of one *.csv-file per snow profile from the DAV and SWISS data sets (in respective subfolders). Each file is named after the profile ID which allows identifying the corresponding observed profile in the "observed_profiles" folder. One row corresponds to one simulated snow layer. The content of the columns 1-7 is described in Table 3 of the ReadMe file. The remaining columns (8-31) contain all features that were used in the development of the RF model and are described in Table 1 of the ReadMe file.

en: Observed snow profiles

en: Observed snow stratigraphy in form of one *.csv-file per snow profile from the DAV and SWISS data sets (in respective subfolders). Each file is named after the profile ID which allows identifying the corresponding simulated profile in the "simulated_profiles" folder. One row corresponds to one observed snow layer. The content of the columns is described in Table 2 of the ReadMe file.

en: DAV data set: Properties of observed snow profiles and simulated failure layers

en:
For each profile pair from the DAV data set, this file contains one row with information on the observed snow stability and all extracted features describing the simulated failure layers. The content of the columns is explained in Table 1 of the ReadMe file. Only those profile pairs which fulfilled predefined similarity criteria (column
"similarity_obs_sim" = 1) and which belonged to the stability classes "stable" and "unstable" (column "stability_class"
= 0 or 1) were used to train the RF model. Please note that for each of the two snow profiles with RB score 7 (profile
IDs 7228 and 9526), three additional layers were included in this data set to obtain a balanced set of training data (73
unstable and 73 stable profiles).

en: ReadMe

en: ReadMe file explaining data structure and content

en: SWISS data set: Properties of observed snow profiles and simulated failure layers

en: For each profile pair from the SWISS data set, this file contains one row with information on the observed snow stability and all extracted features describing the simulated failure layers. Please note that only those profile pairs which fulfilled the similarity criteria (column similarity_obs_sim = 1, 121 profiles) were used to validate the RF model. The content of the columns is explained in Table 1 of the ReadMe file.

en:
This data set includes information on all observed and simulated snow profiles that were used to train and validate the
random forest model described in Mayer et al. (2022). The RF model was trained to assess snow instability from simulated snow stratigraphy. The data set contains observed snow profiles from the region of Davos (DAV subset, 512 profiles) and from all over Switzerland (SWISS subset, 230 profiles). For each observed snow profile, there is a corresponding simulated profile which was obtained using meteorological input data for the numerical snow cover model SNOWPACK. The information on the observed snow profile contains a Rutschblock test result including the depth of the failure interface. As part of the study described in Mayer et al. (2022), each observed snow profile was manually compared to its simulated counterpart and the simulated layer corresponding to the Rutschblock failure layer was identified.

The data are provided in the following form: one file each per observed and simulated snow profile (2x512 files DAV, 2x230 files SWISS), two files (1 file DAV, 1 file SWISS) containing the observed information on snow instability, the allocation between observed and simulated failure layer, and all features extracted from the simulated weak layers that were used to develop the RF model.

failure-layer

display_name: failure-layer

rutschblock

display_name: rutschblock

snow-instability

display_name: snow-instability

snow-profiles

display_name: snow-profiles

snow-stratigraphy

display_name: snow-stratigraphy

snowpack

display_name: snowpack

snowpack-simulation

display_name: snowpack-simulation

education

en: Education and science

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Observed and simulated snow profile data


maintainer: Nander Wever

en: shortwave-radiation humidity snow-ice-temperature wind-direction air-temperature wind-speed longwave-radiation precipitation-amount snow-depth

en: Version 6

media_type: SMET

en:
Deprecated version.

Release notes:

- Missing ISWR for 2009-09-30 and 2010-04-30 has been replaced by data from WFJ Gipfel

This dataset is the basis for the Weissfluhjoch dataset in ESM-SnowMIP (doi: http://dx.doi.org/10.16904/16).

en: SNOWPACK ini file (new syntax)

en: This ini-file can be used to run SNOWPACK simulations with the WFJ data set, using the new MeteoIO Filter, Generator and Interpolation syntax.

en: All versions

en: Version 1

en:
Deprecated version. Release notes:

First release of dataset.

en: Undercatch correction

en: This document describes how the undercatch correction for the Weissfluhjoch measurement site was determined.

en: Version 3

en:
Deprecated version. Release notes:

Bug fix: in version 2, the undercatch correction was not applied correctly

Bug fix: in version 2, snow lysimeter data was missing for extended periods of time

Update: dataset extended to 2016-01-02T00:30

Update: refreshing data originating from MeteoSwiss (particularly precipitation)

en: SNOWPACK ini file (old syntax)

en: This ini-file can be used to run SNOWPACK simulations with the WFJ data set, using version 3.4.0 or higher.

en: Version 4

en:
Deprecated version. Release notes:

Update: dataset extended to 2016-09-02T00:30

Update: refreshing data originating from MeteoSwiss (particularly precipitation)

en: Version 7 (current)

en:
Release notes:

Bug fix: in previous versions, snow lysimeter data from September 2010 onwards was erroneously reported for UTC+2, instead of UTC+1 (i.e., reported one hour later than should have been).

Update: dataset extended to 2017-07-11T00:30.

en: SNOWPACK sno file

en: This sno-file can be used to run SNOWPACK simulations with the WFJ data set, using version 3.4.0 or higher.

en: Version 2

en:
Deprecated version. Release notes:

Bug fix: in v1, incoming radiation components were taken from WFJ_Gipfel station, instead of WFJ Versuchsfeld.

en: Version 5

en:
Deprecated version. Release notes:

Update: using the incoming and outgoing short- and longwave radiation data from the new processing chain from 2010 onwards, filling also data gaps in pre-2010 data.

Update: dataset extended to 2016-10-04T00:30

Update: refreshing data originating from MeteoSwiss (particularly precipitation)

en:
Dataset of meteorological and snowpack measurements from the automatic weather station at Weissfluhjoch, Davos, Switzerland, suitable for driving snowpack models. The dataset contains standard meteorological measurements, and additionally snowpack runoff data from a snow lysimeter. Where possible, data is quality checked and missing data are replaced from backup sensors from the measurement site itself, or (in only a few cases) from the MeteoSwiss weather station at 470 m distance and 150 m above the measurement site.

Publication
Wever, N., Schmid, L., Heilig, A., Eisen, O., Fierz, C., and Lehning, M. Verification of the multi-layer SNOWPACK model with different water transport schemes. 2015. The Cryosphere. Volume 9. 2271-2293. http://dx.doi.org/10.5194/tc-9-2271-2015.

air-temperature

display_name: air-temperature

humidity

display_name: humidity

longwave-radiation

display_name: longwave-radiation

precipitation-amount

display_name: precipitation-amount

shortwave-radiation

display_name: shortwave-radiation

snow-depth

display_name: snow-depth

snow-ice-temperature

display_name: snow-ice-temperature

wind-speed

display_name: wind-speed

envidat

en: EnviDat: the Environmental Data Portal

en: WFJ_MOD: Meteorological and snowpack measurements from Weissfluhjoch, Davos, Switzerland


maintainer: Frank Techel

en: snow danger-level warning-regions european-alps avalanche-forecast

en: Forecast avalanche danger level

en: contains the highest forecast danger level (dangerLevelMax) for each day and warning region

en: shape file warning regions Alps

en:
This dataset contains the data used in the publication by Techel et al., 2018 _Spatial consistency and bias in avalanche forecasts - a case study in the European Alps_ (Nat Haz Earth Syst Sci).
For details on the data please refer to this publication.
The dataset contains the following:
- shape files for the warning regions in the Alps
- highest forecast danger level for each warning region and day

avalanche-forecast

display_name: avalanche-forecast

danger-level

display_name: danger-level

european-alps

display_name: european-alps

snow

display_name: snow

warning-regions

display_name: warning-regions

education

en: Education and science

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Forecast avalanche danger level European Alps 2011 - 2015


en: relative-humidity swiss-forest-lab meteo par long-term meteorology temperature wind-speed precipitation climate-change

en: Forest station data (CIB)

en: Data file.

en: Site photo of the forest station (CIB)

en: Site photo of the forest station from below (viewing upwards the slope).

en: Site photo of the field station (CIF)

en: Site photo of the field station from the side.

en: Data plot of the forest station (CIB)

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

en: Data plot of the field station (CIF)

en: Site aerial photo (Chironico)

en: Site aerial photo from both Chironico stations. Source: Google. 2016. Site aerial photo from both Chironico stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Chironico in Switzerland where one station is located within a natural coniferous forest stand (CIB) with Norway spruce (_Picea abies_; 160-180 yrs) and European silver fir (_Abies alba_; 140-160 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, CIF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Chironico is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

en: Chironico, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 2000 onwards


en: relative-humidity par meteo swiss-forest-lab precipitation long-term climate-change temperature wind-speed meteorology

en: Forest station data (LAB)

en: Site photo of the forest station (LAB)

en: Site photo of the fforest station from the side.

en: Field station data (LAF)

en: Site photo of the field station (LAF)

en: Data plot of the forest station (LAB)

en: Data plot of the field station (LAF)

en: Site aerial photo (Lausanne)

en: Site aerial photo from both Lausanne stations. Source: Google. 2016. Site aerial photo from both Lausanne stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Lausanne in Switzerland where one station is located within a natural mixed forest stand (LAB) with European beech (_Fagus sylvatica_; 160-170 yrs), European silver fir (_Abies alba_; 160-170 yrs) and Norway spruce (_Picea abies_; 160-170 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, LAF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Lausanne is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: Lausanne, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1996 onwards