en: yellowstone carrion fungi soil-biodiversity detritus decomposition biogeochemical-cycling bacteria wolf carcass

en:
Site	ID for different carcasses across the Yellowstone Landscape
Location	Sampling location, either under carcass or control plot
Species	Indicating whether carcass was from bison or elk
Gender	Gener of carcass; F = female, M = male, ? = unknown
Lat	Latitude
Long	Longitude
DSK	Days since kill; in days
Soil.N	Soil N concentration (%)
Soil.C	Soil C concentration (%)
Veg.C	Vegetation C concentration (%); dominant grasses sampled
Veg.N	Vegetation N concentration (%); dominant grasses sampled
Veg.CtoN	Vegetation C to N ratio; dominant grasses sampled
Soil.resp	Soil respiration (g m-2 h-1)
soil.temp	Soil temperature (°C)
Soil.moist	Soil moisture (volumentric; %)
CRR	Cotton rotting rate
bacteria.richness	Bacteria richness
bacteria.diversity	Bacteria diverstiy
fungi.richness	Fungi richness
fungi.diversity	Fungi diversity
bacteria.abundance	Bacteria abundance
fungi.abundance	Fungi abundance

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:

Risch, A.C., Frossard, A., Schütz, M., Frey, B., Morris, A.W., Bump, J.K. (accepted) Effects of elk and bison carcasses on soil microbial communities and ecosystem functions in Yellowstone, USA. (accepted). Functional Ecology
doi: ...

Methods
Study area and study sites
This study was conducted in YNP’s Northern Range (NR), located in north-western Wyoming and south-western Montana, USA (~44.9163° N, 110.4169° W). The NR expands over ~1000 km2 and features long cold winters and short dry summers. Grasslands and shrublands dominate the NR that is the home of large migratory herds of bison (winter counts 2017: ~3919 individuals; Geremia, Wallen, & White, 2017) and elk (~5349 individuals) as well as their main predators, approximately five packs of wolves with a total of 33 individuals (Smith et al., 2017). As part of a long-term research program within YNP, wolf predation has been studied since their reintroduction in 1995.
For our study, we received ground-truthed coordinates of bison and elk carcasses from winter 2016/17 (November 2016 through April 2017) from the YNP Wolf Project. Between June 20 and July 1, 2017, we visited 24 carcasses in total. At five sites, we could not sample as the carcasses were no longer found. In total we located remains (hairmats, rumen content, bones, teeth) of 19 adult male and female carcasses (7 bison, 12 elk; Supplementary Table 1). Live body weights of adult bison and elk are approximately 730 kg (male bison), 450 kg (female bison), 330 kg (male elk), and 235 kg (female elk, Meagher, 1973; Quimby & Johnson, 1951).
The kills and subsequent consumption happened between 34 and 173 days prior to our sampling (hereafter “days since kill”, DSK), for which we accounted in our statistics. Note that wolves and other scavengers consumed the soft tissue of the carcasses quickly, hence, there is close to no soft tissue left for decomposition as compared to an intact body left on the soil surface. The 19 carcass sites covered the extent of YNP’s NR, with both bison and elk carcasses showing similar distributions; elevation ranged from 1703 to 2884 m a.s.l. (Supplementary Fig 1 & Supplementary Table 1). The carcasses were all located in grassland or sage-brush shrubland, with or without sparsely scattered trees, and both bison and elk carcasses showed the same distribution of DSK. At each study site, we selected a reference plot (hereafter “control”) that was of comparable size, slope aspect and vegetation to the carcass location (hereafter “carcass”). The control was at least 10 m away (Danell, Berteaux, & Brathen, 2002; Melis et al., 2007) from the carcass itself to ensure the absence of potential direct and indirect carcass effects (paired design; (Bump, Webster, et al., 2009; Bump, Peterson, et al., 2009).

Ecosystem functions and soil properties
We randomly collected 50 g of mineral soil from three locations on both control and carcass plots to a depth of 5 cm with sterile techniques and gently mixed the material to obtain a composite sample. Half the soil sample was immediately bagged in plastic bags (whirl packs), stored in a cooler with ice packs (~5 ºC), sieved (2-mm) and frozen within 4-6 hours of collection to assess soil microbial communities. For this purpose, we extracted total genomic DNA from 0.5 g soil using the PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany). DNA concentrations were measured using PicoGreen (Molecular Probes, Eugene, OR, USA). PCR amplifications of partial bacterial small-subunit ribosomal RNA genes (region V3–V4 of 16S rRNA) and fungal ribosomal internal transcribed spacers (region ITS2) were performed as described previously (Frey et al., 2016). Each sample consisting of 40 ng DNA was amplified in triplicate and pooled before purification with Agencourt AMPure XP beads (Beckman Colter, Berea, CA, USA) and quantified with the Qubit 2.0 fluorometric system (Life Technologies, Paisley, UK). Amplicons were sent to the Genome Quebec Innovation Center (Montreal, Canada) for barcoding using the Fluidigm Access Array technology and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA).
Quality control of bacterial and fungal reads was performed using a customized pipeline (Supplementary Table 2; Frey et al., 2016). Paired-ends reads were matched with USEARCH (Edgar & Flyvbjerg, 2015), substitution errors were corrected using Bayeshammer (Nikolenko, Korobeynikov, & Alekseyev, 2013) and PCR primers were trimmed (allowing for 1 mismatch, read length >300 bp for 16S and >200 bp for ITS primers) using Cutadapt (M. Martin, 2011). Sequences were dereplicated and singleton reads removed prior to clustering into operational taxonomic units (OTUs) at 97% identity using USEARCH (Edgar, 2013). The remaining centroid sequences were tested for the presence of ribosomal signatures using Metaxa2 (Bengtsson-Palme et al., 2015) or ITSx (Bengtsson-Palme et al., 2013). Taxonomic assignments of the OTUs were obtained using Bayesian classifier (Wang, Garrity, Tiedje, & Cole, 2007) with a minimum bootstrap support of 60% implemented in mothur (Schloss et al., 2009) by querying the bacterial and fungal reads against the SILVA Release 128 (Quast et al., 2013) and UNITE 8.0 (Abarenkov et al., 2010) reference databases for 16S and ITS OTUs, respectively.
Abundances of the bacterial 16S rRNA gene and fungal ITS amplicon were determined by quantitative real-time PCR (qPCR) on an ABI7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) as described previously (Frossard et al., 2018). The same primers (without barcodes) and cycling conditions as for the sequencing approach were used for the 16S and ITS qPCR. Three standard curves per target region were obtained using tenfold serial dilutions of plasmids generated from cloned targets (Frey, Niklaus, Kremer, Lüscher, & Zimmermann, 2011). Data were converted to represent mean copy number of targets per gram of soil (dry weight).
The other half of the soil sample was bagged in paper, dried to constant weight at 60°C, passed through a 2 mm sieve and analyzed for total C and N concentration with a CE Instruments NC 2100 soil analyzer (CE Elantech Inc., Lakewood NJ, USA). We also collected 20 mature and undamaged leaves of the dominant grass species growing on control and carcass sites, but taxa were not recorded. The plant material was dried at 60°C, finely ground till homogenized and also analyzed to obtain total C and N concentrations. Soil temperature (10 cm depth) was measured with a waterproof digital thermometer (Barnstead International, Dubuque IA, USA) at three locations each at the control and carcass site. Soil moisture (0 – 10 cm depth) was measured with time domain reflectometry (Field-Scout TDR-100; Spectrum Technologies, Plainfield IL, USA) at five randomly chosen points on control and carcass sites. We measured soil respiration at five randomly chosen points at both control and carcass sites with a PP-Systems SRC-1 soil respiration chamber (closed circuit) attached to a PP-Systems EGM-4 infrared gas analyzer (PP-Systems, Amesbury, MA, USA). For each measurement the soil chamber (15 cm high; 10 cm diameter) was tightly placed on the soil surface, after clipping plants to avoid measuring plant respiration or photosynthesis. Measurements were conducted over 120 s.
In addition, we assessed the decomposition rates of standardized OM using the cotton strip assay (Latter & Howson, 1977; Latter & Walton, 1988). Cotton cloth tensile strength loss (CTSL) is a measure of decomposition, and an index to express the combined effect of soil microclimatic, physical, chemical and biological properties on decomposition while accounting for OM quality (Latter & Walton, 1988; Risch, Jurgensen, & Frank, 2007; Withington & Sanford Jr., 2007). We placed five 20 cm wide x 13 cm long sheets of 100% unbleached cotton cloth (American Type SM 1/18’’, Warp: 34/1, Weft: 20/1, Weave plain, 29.5 picks/cm warp, 22 picks/cm weft, 237 g/m2; Daniel Jenny & Co., Switzerland;) at each carcass and control site vertically into the soil by making slits with a flat spade to a depth of 12 cm. We inserted each cloth with the spade, and then pushed the slit closed to assure tight contact with the soil. The cloths were retrieved after 18 to 27 days. After retrieval, the cloths were air-dried, remaining soil gently removed by hand, and 1.5 cm wide strips were cut at the 3.5-5.0 cm (top) and the 9-10.5 cm (bottom) soil depth. The strips were equilibrated at 50 % relative humidity and 20°C for 48 hours (climate chamber) prior to strength testing (Scanpro Awetron TH-1 tensile strength tester; AB Lorentzen and Wettre, Kista, Sweden). Cotton rotting rate (CRR) = (CTScontrol - CTSfinal/CTSfinal)1/3 * (365/t), where CTScontrol is the cotton tensile strength of a control cloth and CTSfinal the cotton tensile strength of the incubated sample, t is the incubation period in days. Control cloths were inserted into the ground and immediately retrieved to account for tensile strength loss associated with cloth insertion. We averaged the CRR of top and bottom strips for further analyses as no difference was found between the two. All sampling and cloth insertion took place between June 20 and July 1, 2017, cloths were retrieved between July 17 and 20, 2017. Soil respiration, average CRR, vegetation N concentration and vegetation C:N ratio are defined as ecosystem functions, soil C and N concentration, soil temperature and moisture as soil abiotic properties, and bacterial and fungal richness (number of taxa), diversity (Shannon) and abundance as soil biotic properties.

Statistical analyses
Univariate analyses for ecosystem functions, soil biotic and abiotic properties
We tested whether individual ecosystem functions, soil biotic and abiotic properties differed between carcass and control (“Location”), bison and elk (“Species”) and days since kill (“DSK”). For this purpose, we used linear mixed effect models (LMM, “nlme” package v 3.1 – 131.1 in R v 3.4.4; Pinheiro, Bates, DebRoy, & Sarkar, 2018; R Core Team, 2019) with Location, Species, Location x Species and DSK as fixed effects. Site was included as random effect to account for the paired design. We developed a separate model for all dependent variables. All but bacterial richness, fungal richness, fungal diversity and vegetation N concentration were natural-log transformed to meet model assumptions. For each LMM, we calculated contrasts to assess the specific comparisons we were interested in with the “lsmeans” package v 2.27-62 (Lenth & Love, 2018): 1) carcass vs control, 2) carcass bison vs control bison, and 3) carcass elk vs control elk. We also tested whether we had differences between bison and elk carcasses or the sites where bison and elk were killed and included contrasts 4) carcass bison vs carcass elk and 5) control bison vs control elk.
We calculated the log response ratio (LRR = ln[carcass/control]) to obtain carcass effects for all variables for both species separately. LRR < 0 indicates higher value at control compared to carcass, LRR > 0 indicates higher values at carcass compared control. We used LRRs for visualization and to assess spatial patterns in carcass effects across YNP. For this purpose we calculated the Moran’s I statistic for each ecosystem function, soil biotic and abiotic property based on a latitude-longitude matrix with the “moran.test” function in the “spdep” package version 1.1-3 (Bivand et al., 2019).
Multivariate analyses
Rare OTUs, defined as OTUs with a low abundance of reads, were retained in multivariate methods because they only marginally influence these analyses (Gobet, Quince, & Ramette, 2010). Bray–Curtis dissimilarity matrices were generated based on square-root-transformed matrices. We used Principal Coordinate Analyses (PCoA) to assess how soil bacterial and fungal communities differed between control and carcass of bison and elk (“vegan” package v 2.5-4, Oksanen et al., 2019). We then extracted PCoA axes scores 1 and 2 and used LMM (“nlme” package) with Location, Species, Location x Species and DSK as fixed effects. Site was, again, included as random effect. We again calculated the contrasts as described above using the “lsmeans” package. We also assessed how ecosystem functions, and soil abiotic and biotic properties were related to the soil bacteria and fungi community structure associated with bison and elk control and carcasses using the “envfit” function in the “vegan” package (Oksanen et al., 2019).
Indicator species analyses were performed using the multipatt function implemented in the “indicspecies” package version 1.7.6 with 100000 permutations (De Caceres & Jansen, 2016). This step allowed to identify OTUs that led to changes in multivariate patterns between control and carcass of both bison and elk separately (De Cáceres, Legendre, & Moretti, 2010). The multipatt function uses a point biserial correlation coefficient statistical test. Indicator OTUs were defined as bacterial and fungal OTUs with more than 50 sequences, i.e., removing rare taxa and taxa with low abundances containing little indicator information (Rime et al., 2015) and that were significantly correlated with Location (p < 0.05, correlation coefficient > 0.3). A heatmap of these OTUs were generated with the vegan and ggplot2 packages. The indicator analyses were performed in R version 3.3.3 (R Core Team, 2017).

References
Abarenkov, K., Henrik Nilsson, R., Larsson, K.-H., Alexander, I. J., Eberhardt, U., Erland, S., … Kõljalg, U. (2010). The UNITE database for molecular identification of fungi – recent updates and future perspectives. New Phytologist, 186(2), 281–285. doi:10.1111/j.1469-8137.2009.03160.x
Bengtsson-Palme, J., Hartmann, M., Eriksson, K. M., Pal, C., Thorell, K., Larsson, D. G. J., & Nilsson, R. H. (2015). metaxa2: improved identification and taxonomic classification of small and large subunit rRNA in metagenomic data. Molecular Ecology Resources, 15(6), 1403–1414. doi:10.1111/1755-0998.12399
Bengtsson-Palme, J., Ryberg, M., Hartmann, M., Branco, S., Wang, Z., Godhe, A., … Nilsson, R. H. (2013). Improved software detection and extraction of ITS1 and ITS2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data. Methods in Ecology and Evolution, 4(10), 914–919. doi:10.1111/2041-210X.12073
Bivand, R., Altman, M., Anselin, L., Assuncao, R., Berke, O., Blanchet, G., … Yu, D. (2019). spdep: Spatial dependence, weighthing schemes, statistics. R package version 1.1-3.
Bump, J. K., Peterson, R. O., & Vucetich, J. A. (2009). Wolves modulate soil nutrient heterogeneity and foliar nitrogen by configuring the distribution of ungulate carcasses. Ecology, 90(11), 3159–3167.
Bump, J. K., Webster, C. R., Vucetich, J. A., Peterson, R. O., Shields, J. M., & Powers, M. D. (2009). Ungulate carcasses perforate ecological filters and create biogeochemical hotspots in forest herbaceous layers allowing trees a competitive advantage. Ecosystems, 12(6), 996–1007. doi:10.1007/s10021-009-9274-0
Danell, K., Berteaux, D., & Brathen, K. A. (2002). Effect of muskox carcasses on nitrogen concentration in tundra vegetation. Arctic, 55(4), 389392.
De Caceres, M., & Jansen, F. (2016). indicspecies: relationship between species and groups of species. R package version 1.7.6.
De Cáceres, M., Legendre, P., & Moretti, M. (2010). Improving indicator species analysis by combining groups of sites. Oikos, 119(10), 1674–1684. doi:10.1111/j.1600-0706.2010.18334.x
Edgar, R. C. (2013). UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods, 10, 996.
Edgar, R. C., & Flyvbjerg, H. (2015). Error filtering, pair assembly and error correction for next-generation sequencing reads. Bioinformatics, 31(21), 3476–3482. doi:10.1093/bioinformatics/btv401
Frey, B., Niklaus, P. A., Kremer, J., Lüscher, P., & Zimmermann, S. (2011). Heavy-machinery traffic impacts methane emissions as well as methanogen abundance and community structure in oxic forest soils. Applied and Environmental Microbiology, 77(17), 6060–6068. doi:10.1128/AEM.05206-11
Frey, B., Rime, T., Phillips, M., Stierli, B., Hajdas, I., Widmer, F., & Hartmann, M. (2016). Microbial diversity in European alpine permafrost and active layers. FEMS Microbial Ecology, 92(3), fiw018.
Frossard, A., Donhauser, J., Mestrot, A., Gygax, S., Bååth, E., & Frey, B. (2018). Long- and short-term effects of mercury pollution on the soil microbiome. Soil Biology and Biochemistry, 120, 191–199. doi:https://doi.org/10.1016/j.soilbio.2018.01.028
Geremia, C., Wallen, R., & White, P. J. (2017). Status report of the Yellowstone bison population, September 2017. Yellowstone National Park, Mammoth, WY, USA: National Park Service, Yellowstone Center for Resources.
Gobet, A., Quince, C., & Ramette, A. (2010). Multivariate cutoff level analysis (MultiCoLA) of large community data sets. Nucleic Acids Research, 38(15), e155–e155. doi:10.1093/nar/gkq545
Latter, P., & Howson, G. (1977). The use of cotton strips to indicate cellulose decomposition in the field. Pedobiologia, (17), 145–155.
Latter, P., & Walton, D. (1988). The cotton strip assay for cellulose decomposition studies in soil: history of the assay and development. In Cotton strip assay: an index for decomposition in soils (pp. 7–9). ITE Symposium, Institute of Terrestrial Ecology, Natural Environment Research Council, UK.
Lenth, R., & Love, J. (2018). lsmeans: least-squares means. R package version 2.27-62.
Martin, M. (2011). Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.Journal, 17(1), 10–12.
Meagher, M. M. (1973). The bison of Yellowstone National Park. NPS Scientific Monograph (Vol. 1). National Park Service, Yellowstone Center for Resources.
Melis, C., Selva, N., Teurlings, I., Skarpe, C., Linnell, J. D. C., & Andersen, R. (2007). Soil and vegetation nutrient response to bison carcasses in Białowieża Primeval Forest, Poland. Ecological Research, 22(5), 807–813. doi:10.1007/s11284-006-0321-4
Nikolenko, S. I., Korobeynikov, A. I., & Alekseyev, M. A. (2013). BayesHammer: Bayesian clustering for error correction in single-cell sequencing. BMC Genomics, 14(1), S7. doi:10.1186/1471-2164-14-S1-S7
Oksanen, J., Blanchet, F. G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., … Wagner, H. H. (2019). vegan: community ecology package. R package version 2.5-4.
Pinheiro, J., Bates, D., DebRoy, S., & Sarkar, D. (2018). nlme: Linear and nonlinear mixed effect models. R package version 3.1-131.1.
Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., … Glöckner, F. O. (2013). The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Research, 41(Database issue), D590–D596. doi:10.1093/nar/gks1219
Quimby, D. C., & Johnson, D. E. (1951). Weights and measurements of Rocky Mountain elk. Journal of Wildlife Management, 15, 57–62.
R Core Team. (2017). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Zurich, Switzerland.
R Core Team. (2019). R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing.
Rime, T., Hartmann, M., Brunner, I., Widmer, F., Zeyer, J., & Frey, B. (2015). Vertical distribution of the soil microbiota along a successional gradient in a glacier forefield. Molecular Ecology, 24(5), 1091–1108. doi:10.1111/mec.13051
Risch, A. C., Jurgensen, M. F., & Frank, D. A. (2007). Effects of grazing and soil micro-climate on decomposition rates in a spatio-temporally heterogeneous grassland. Plant and Soil, 298(1–2), 191–201. doi:10.1007/s11104-007-9354-x
Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., … Weber, C. F. (2009). Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology, 75(23), 7537–7541. doi:10.1128/AEM.01541-09
Smith, D., Stahler, D., Cassidy, K., Stahler, E., Metz, M., Cassidy, B., … Cato, E. (2018). Yellowstone National Park wolf project annual report 2017. Yellowstone National Park, Mammoth, WY, USA: National Park Service, Yellowstone Center of Resources.
Wang, Q., Garrity, G. M., Tiedje, J. M., & Cole, J. R. (2007). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology, 73(16), 5261–5267. doi:10.1128/AEM.00062-07
Withington, C., & Sanford Jr., R. (2007). Decomposition rates of buried substances increase with altitude in a forest-alpine tundra ecotone. Soil Biology and Biochemistry, (39), 68–75.

Please cite this paper together with the citation for the datafile.

bacteria

display_name: bacteria

biogeochemical-cycling

display_name: biogeochemical-cycling

carcass

display_name: carcass

carrion

display_name: carrion

decomposition

display_name: decomposition

detritus

display_name: detritus

fungi

display_name: fungi

soil-biodiversity

display_name: soil-biodiversity

wolf

display_name: wolf

yellowstone

display_name: yellowstone

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Elk and bison carcasses in Yellowstone, USA


snow-drift-station-snow-and-air-data

maintainer: Benjamin Walter

title_for_slug: snow-drift-station-snow-and-air-data

en: snow-depth snow-temperature snow-height snow raclets temperature

state: active

type: dataset

en: Snow Surface Temperature

en: The snow surface temperature (°C) was measured with a Campbell SI-111 at 0.7 m above ground. The time is given in UTC+1.

format: XLS

en: Snow Depth

en: The snow depth (cm) was measured with a Lufft SHM-31 which was mounted at 1.7 m above ground. The time is given in UTC+1.

en: Air Temperature

en: The air temperature (°C) was measured with a Campbell CS-215 which was mounted at a height of 2.2 m above ground. The time is given in UTC+1.

en: Relative Humidity

en: The relative humidity (%) was measured with a Campbell CS-215 at a height of 2.2 m above ground. The time is given in UTC+1.

en: Snow and air data was monitored at Gotschnagrat (LON: 46.859 LAT: 9.849) by an infrarot radiometer (Campbell SI-111) for snow temperature (°C), a snow height sensor (Lufft SHM-31) for snow height change (cm) and a temperature and humidity sensor (Campbell CS-215) for air temperature (°C) and relative humidity (%). No filter was applied to the sensors and the smapling frequency was 1 Hz.

raclets

display_name: raclets

snow

display_name: snow

snow-depth

display_name: snow-depth

snow-height

display_name: snow-height

snow-temperature

display_name: snow-temperature

temperature

display_name: temperature

education

fr: Education, science

de: Bildung, Wissenschaft

en: Education and science

it: Formazione e scienza

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: Snow Drift Station - Snow and Air Data


maintainer: EnviDat Support

en: air-temperature paleo-climate last-glacial-maximum precipitation glaciers orography

en: Technical documentation

media_type: application/pdf

en: Technical documentation containing file metadata and variable descriptions.

format: PDF

mimetype: application/pdf

en: Data Access via S3

media_type: geotiff

en:
High resolution, downscaled climate model data are used in a wide variety of applications in environmental sciences. Here we present the CHELSA-TraCE21k downscaling algorithm to create global monthly climatologies for temperature and precipitation at 30 arcsec spatial resolution in 100 year time steps for the last 21,000 years. Paleo orography at high spatial resolution and at each timestep is created by combining high resolution information on glacial cover from current and Last Glacial Maximum (LGM) glacier databases with the interpolation of a dynamic ice sheet model (ICE6G) and a coupling to mean annual temperatures from CCSM3-TraCE21k. Based on the reconstructed paleo orography, mean annual temperature and precipitation was downscaled using the CHELSA V1.2 algorithm.

The data is published under a Creative Commons Attribution 2.0 Generic (CC BY 2.0) license.

format: GeoTIFF

media_type: .pdf

format: SERVICE

air-temperature

display_name: air-temperature

glaciers

display_name: glaciers

last-glacial-maximum

display_name: last-glacial-maximum

orography

display_name: orography

paleo-climate

display_name: paleo-climate

precipitation

display_name: precipitation

en: CHELSA-TraCE21k: Downscaled transient temperature and precipitation data since the last glacial maximum


en: geoinformation-software digital-planning digital-elevation-model swiss-geodata seilaplan open-source qgis-plugin cable-yarding swisstopo

en: View video on youtube!

en: Herunterladen des DHM von swisstopo Website

en:
In order to use the QGIS plugin ‘Seilaplan’ for digital cable line planning, a digital terrain model (DTM) is required. As an alternative to using the ‘Swiss Geo Downloader’ plugin, the DTM can be obtained directly from Swisstopo. In this tutorial we explain step by step how to download the necessary DTM from the Swisstopo Website, and how to use it in QGIS for the digital planning of a cable line using the plugin ‘Seilaplan’. Please note that the tutorial language is German!

Link to the elevation model on the swisstopo website: https://www.swisstopo.admin.ch/de/geodata/height/alti3d.html#technische_details

Link to the rope map website: https://seilaplan.wsl.ch

********************

Für die Verwendung des QGIS Plugins Seilaplan zur digitalen Seillinienplanung ist ein digitales Höhenmodell (DHM) nötig. Als Alternative zum Swiss Geo Downloader erklären wir in diesem Tutorial Schritt für Schritt, wie man das nötige Höhenmodell von der Swisstopo Webseite herunterladen und in QGIS zur Seillinienplanung verwenden kann.

Link zum Höhenmodell auf der swisstopo Webseite: https://www.swisstopo.admin.ch/de/geodata/height/alti3d.html#technische_details

Link zur Seilaplan-Website: https://seilaplan.wsl.ch

cable-yarding

display_name: cable-yarding

digital-elevation-model

display_name: digital-elevation-model

digital-planning

display_name: digital-planning

geoinformation-software

display_name: geoinformation-software

open-source

display_name: open-source

qgis-plugin

display_name: qgis-plugin

seilaplan

display_name: seilaplan

swiss-geodata

display_name: swiss-geodata

swisstopo

display_name: swisstopo

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Seilaplan Tutorial: DTM download from swisstopo website


maintainer: Thorsten Bartels-Rausch

en: polar antartic sea-salt-aerosol snow laboratory-study cryosphere arctic

en: Experimental results of uptake coefficient vs. temperature and ozone concentration

media_type: text/csv

en:
Data to Figure 3: Measured uptake coefficients as a function of temperature at 5E12 molecules per cm3 ozone concentration.

Data to Figure 6: Measured uptake coefficients at different temperatures as a function of gas- phase ozone concentration.

format: CSV

mimetype: text/csv

en: Experimental Results: Uptake coefficients vs. temperature and ozone concentration

en:
* Final Data to Figure 3: Uptake coefficients as a function of temperature at 5E12 molecules per cm3 ozone concentration.
* Final Data to Figure 6: Uptake coefficients at different temperatures as a function of gas- phase ozone concentration.

The average ozone uptake coefficients of the three cycles between the bypass and the flow tube per repeat is taken as the mean uptake coefficient for each experiment. Gamma was corrected for gas phase diffusion limitation following the Cooney–Kim– Davis (CKD) method52 (see supporting material of publication for details).

en: The reaction of ozone with bromide in polar regions results in the formation of reactive bromide species with impacts on ozone budget and the oxidative capacity of the lower atmosphere. Here, we present a data investigating the temperature dependence of bromide oxidation by ozone using a coated wall flow tube reactor coated with an aqueous mixture of citric acid and sodium bromide, a proxy for sea salt aerosol in snow or the free troposphere. Thus study shows the effect of of organic species at relatively mild temperatures between the freezing point and eutectic temperature as typical for Earth's cryosphere.

antartic

display_name: antartic

arctic

display_name: arctic

cryosphere

display_name: cryosphere

laboratory-study

display_name: laboratory-study

polar

display_name: polar

sea-salt-aerosol

display_name: sea-salt-aerosol

snow

display_name: snow

en: Kinetic experiments on the oxidation of bromide by ozone from 289-245 K.


mountain-permafrost-hydrology

maintainer: Jeannette Noetzli

title_for_slug: mountain-permafrost-hydrology

en: climate-change-impacts permafrost-degradation monitoring-techniques mass-movements ice-rich-permafrost mountain-permafrost-hydrology

en: Synthesis report chapter of the Hydro-CH2018 project of the Federal Office for the Environment (FOEN)

media_type: PDF

en: DORA Persistent URL

en: This report was prepared as one of the synthesis report chapters of the Hydro-CH2018 project of the Federal Office for the Environment (FOEN). In earlier reports such as the CH2014-IMPACTS report (CH-Impacts 2014), the topic of mountain permafrost hydrology was not addressed. Here, we provide a baseline of the available knowledge of mountain permafrost in the Swiss Alps for future reference. We compile an overview of the current understanding of mountain permafrost in the Swiss Alps, its distribution and characteristics, observed and projected changes, and expected impacts on slope stability, infrastructure and hydrological aspects. We also briefly describe the measurement techniques and modelling approaches applied. The chapter closes with a summary of the most important open research questions. The literature cited mainly includes studies on mountain permafrost published in scientific journals and assessments of long-term observation data. We focus on permafrost hydrology interactions wherever information is available. However, systematic studies on permafrost hydrology in mountain areas are still limited.

climate-change-impacts

display_name: climate-change-impacts

ice-rich-permafrost

display_name: ice-rich-permafrost

mass-movements

display_name: mass-movements

monitoring-techniques

display_name: monitoring-techniques

mountain-permafrost-hydrology

display_name: mountain-permafrost-hydrology

permafrost-degradation

display_name: permafrost-degradation

education

en: Education and science

en: Mountain Permafrost Hydrology


maintainer: Ana Beatriz Pierri Daunt

en: green-infrastructure sense-of-place landscape-services pdfsearch landscape-perception ecosystem-services decision-making resilience landscape-ecology multifunctionality socio-ecological-system literature-review planning landscape-planning

en: 5. Planning steps.

media_type: CSV

en: 4. Use of any additional concept.

en: 6. Protocol

media_type: XLSX

en: 3. Use of any early concept.

en: 2. Frequency of additional concepts.

en: 1. Frequency of early concepts.

en:
Table of content: 1. Frequency of early concepts; 2. Frequency of additional concepts; 3. Use of any early concept; 4. Use of any additional concept, 5. Planning steps; 6. Protocol.

The present dataset is part of the published scientific paper entitled “Landscape ecological concepts in planning: review of recent developments” (Hersperger et al., 2021). The goal of this research was to review recent publications to assess the use of landscape ecological concepts in planning. Specifically, we address the following research questions: Q1. Landscape ecological concepts: What are they? How frequently are they mentioned in current research? Q2. How are landscape ecological concepts integrated in landscape planning?

We analysed all empirical and overview papers that have been published in four key academic journals in the field of landscape ecology and landscape planning in the years 2015–2019 (n = 1918). Four key journals in the field of landscape ecology were selected to conduct the analysis, respectively Landscape Ecology (LE), Landscape Online (LO), Current Landscape Ecology Reports (CLER), and Landscape and Urban Planning (LUP). The title, abstract and keywords of all papers were read in order to identify landscape ecological concepts. Then, all 1918 papers went through a keyword search to identify the use of early and additional concepts. We used the “pdfsearch” package in R programming language and searched for singular and plural forms and different variations of the concepts (see Supplementary material 1, Table A). As a result, we provided four outputs:

  1. Frequency of early concepts. This data provides the total number of times each article used each early concept (Q1). This data was used to produce the Figure 2a at the original publication.

  2. Frequency of additional concepts. This data provides the total number of times each article used each additional concept (Q1). This data was used to produce the Figure 2b at the original publication.

  3. Use of any early concept. This data provides the total number of times each article used any early concept (Q1).
This data was used to produce the Figure 3a at the original publication.

  4. Use of any additional concept. This data provides the total number of times each article used any additional concept (Q1). This data was used to produce the Figure 3b at the original publication.

To address the second question (Q2), the title, abstract and keywords of the papers included in our sample (n=1918 articles) were screened to identify papers that might show how landscape ecological concepts are integrated into planning. We selected 52 empirical papers (see Supplementary material – 4 Integration of landscape ecological concepts into planning), and we provided two outputs:

  5. Planning steps. This data provides the number of times landscape ecological concepts were addressed in each
planning steps in 52 empirical papers analysed in detail (Q2). This data was used to produce the Figure 4 at the
original publication.

  6. Protocol for assessing the integration of landscape ecological concepts into planning. To systematically collect the
data, we used this protocol which addressed the following questions: (a) which type of planning is addressed by the
paper? (b) to which planning level does the paper refer to? (c) which concepts are integrated in any of the planning
steps described above?

decision-making

display_name: decision-making

ecosystem-services

display_name: ecosystem-services

green-infrastructure

display_name: green-infrastructure

landscape-ecology

display_name: landscape-ecology

landscape-perception

display_name: landscape-perception

landscape-planning

display_name: landscape-planning

landscape-services

display_name: landscape-services

literature-review

display_name: literature-review

multifunctionality

display_name: multifunctionality

pdfsearch

display_name: pdfsearch

planning

display_name: planning

resilience

display_name: resilience

sense-of-place

display_name: sense-of-place

socio-ecological-system

display_name: socio-ecological-system

en: The usage of landscape ecological concepts in the planning literature


en: snow-water-equivalent snow-density weisfluhjoch snow-ice-temperature snow-stratigraphy

en: README

media_type: TXT

en: README file giving use metadata.

format: TXT

en: WFJ Snow Profiles 1999-2014

media_type: application/zip

en:
Biweekly manual snow profiles from Weissfluhjoch, Davos, Switzerland.
Version: 2015-09-14T16:25

format: ZIP

mimetype: application/zip

en: Dataset of manual bi-weekly snow profiles from Weissfluhjoch, Davos, Switzerland. Typical snow profile measurements and observations are included (temperature, density, grain size, grain type, hardness, wetness), following the guidelines of the The International Classification for Seasonal Snow on the Ground (ICSSG) [Fierz, C., Armstrong, R.L., Durand, Y., Etchevers, P., Greene, E., McClung, D.M., Nishimura, K., Satyawali, P.K. and Sokratov, S.A. 2009. The International Classification for Seasonal Snow on the Ground. IHP-VII Technical Documents in Hydrology N°83, IACS Contribution N°1, UNESCO-IHP, Paris].

snow-density

display_name: snow-density

snow-ice-temperature

display_name: snow-ice-temperature

snow-stratigraphy

display_name: snow-stratigraphy

snow-water-equivalent

display_name: snow-water-equivalent

weisfluhjoch

display_name: weisfluhjoch

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Manual bi-weekly snow profiles from Weissfluhjoch, Davos, Switzerland


maintainer: Elisabeth Hafner

en: hazard-mapping avalanche satellite natural-hazard snow

en: SPOT6 Avalanche Outlines 16 January 2019

media_type: SHP

en: Shapefile of the manually mapped avalanche outlines. Please only use this data together with the example key document provided with this dataset and within the limitations of the dataset described in the publications.

format: SHP

en:
Outlines of 6'041 avalanches mapped from SPOT6 satellite data over the Swiss Alps on 16 January 2019. The dataset was acquired following a period with very high avalanche danger. The outlines have different attributes described in the data example key (ExampleKey_AvalMapping16012019.pdf)

The generation of the data is described in:

Bühler, Y., E. D. Hafner, B. Zweifel, M. Zesiger, and H. Heisig (2019), Where are the avalanches? Rapid SPOT6 satellite data acquisition to map an extreme avalanche period over the Swiss Alps, The Cryosphere, 13(12), 3225-3238, doi:10.5194/tc-13-3225-2019.

The data was comprehensivly validated in a subset area in

Hafner, E.D.; Techel, F.; Leinss, S.; Bühler, Y., 2021: Mapping avalanches with satellites - evaluation of performance and completeness. Cryosphere, 15, 2: 983-1004. doi: 10.5194/tc-15-983-2021

avalanche

display_name: avalanche

hazard-mapping

display_name: hazard-mapping

natural-hazard

display_name: natural-hazard

satellite

display_name: satellite

snow

display_name: snow

education

en: Education and science

en: SPOT6 Avalanche outlines 16 January 2019


data-broedlin-cnp

title_for_slug: data-broedlin-cnp

en: stoichiometry carbon dissolved-organic-matter drying-rewetting mineralization phosphorus nitrogen forest soil

en: Data Broedlin-CNP

en: Data Broedlin-CNP-Release

en: Raw data of C-N-P mobilization experiment from Oi, Oe/Oa, and A horizons of beech forest soils along a nutrient availability gradient.

en: Mircocosm experiment to identify the individual patterns and controls of C, N, and P mobilization in soils under beech forests. Organic and mineral horizons sampled along a nutrient availability gradient in Germany were exposed to either permanent moist conditions or to dry spells in microcosms and quantified the release of inorganic and organic C, N, and P.

carbon

display_name: carbon

dissolved-organic-matter

display_name: dissolved-organic-matter

drying-rewetting

display_name: drying-rewetting

forest

display_name: forest

mineralization

display_name: mineralization

nitrogen

display_name: nitrogen

phosphorus

display_name: phosphorus

soil

display_name: soil

stoichiometry

display_name: stoichiometry

en: Data Broedlin CNP


maintainer: Jana von Freyberg

en: stable-water-isotopes plant-water-uptake endmember-mixing-models root-zone ecohydrology uncertainties review

en: Data set of MEE-20-04-264

en: The following two tables contain information about the data sources of the values reported in Table 1 and 2 in the paper “Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources” published in the journal ‘Methods in Ecology and Evolution’.

en: Data set of MEE-20-04-264 (CSV)

en: The following two tables contain information about the data sources of the values reported in Table 1 and 2 in the paper “Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources” published in the journal 'Methods in Ecology and Evolution'.

ecohydrology

display_name: ecohydrology

endmember-mixing-models

display_name: endmember-mixing-models

plant-water-uptake

display_name: plant-water-uptake

review

display_name: review

root-zone

display_name: root-zone

stable-water-isotopes

display_name: stable-water-isotopes

uncertainties

display_name: uncertainties

en: Data set of: Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources


maintainer: Matthias Haeni

en: par meteo precipitation wind-speed long-term swiss-forest-lab meteorology relative-humidity climate-change temperature

en: Site photo of the forest station (BTB)

media_type: image/jpeg

en: Site photo of the forest station from the side.

mimetype: image/jpeg

en: Data plot of the forest station (BTB)

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

en: Site photo of the field station (BTF)

en: Site photo of the field station from the side.

en: Field station data (BTF)

en: Data file.

en: Site aerial photo (Bettlachstock)

en: Site aerial photo from both Bettlachstock stations. Source: Google. 2016. Site aerial photo from both Bettlachstock stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Data plot of the field station (BTF)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Bettlachstock in Switzerland where one station is located within a natural mixed forest stand (BTB) with European beech (_Fagus sylvatica_; 170-190 yrs), European silver fir (_Abies alba_; 190 yrs) and Norway spruce (_Picea abies_; 200 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, BTF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Bettlachstock is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Bettlachstock, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


maintainer: Michael Lehning

en: terrestrial-laser-scanning snow-conservation snow-farming snowpack snow winter-tourism

en: Martell .smet

media_type: SMET

en: Meteodata used as input for SNOWAPCK simulations, orginating from Station Hintermartell.

en: Fluela .smet

en: Meteodata used as input for SNOWAPCK simulations, orginating from Station Windtunnel and DAV.

en: HS Fluela spring 2015

media_type: text/plain

en:
Snow depth/ height of snow heap in spring 2015 obtained by terrrestrial laserscanning
cell size: 10 cm
spatial reference: CH1903

mimetype: text/plain

en: HS Martell spring 2015

en:
Snow depth/ height of snow heap in spring 2015 obtained by terrestrial laserscanning
cell size: 10 cm
spatial reference: European Datum 1950 UTM
Nodata: -9999

en: Martell .sno

media_type: SNO

en: Input snow profile (.sno file) used for SNOWPACK simulations of the Martell heap

en: HS Fluela autumn 2015

en:
Snow depth/ height of snow heap in autumn 2015 obtained by terrestrial laserscanning
cell size: 10 cm
spatial reference: CH1903
Nodata: -9999

en: Fluela .sno

en: Input snow profile (.sno file) used for SNOWPACK simulations of the Flüela heap

en: HS Martell autumn 2015

en:
Snow depth/ height of snow heap in autumn 2015 obtained by terrestrial laserscanning
cell size: 10 cm
spatial reference: European Datum 1950 UTM
Nodata: -9999

en:
Two data sets obtained for snow farming projects (Fluela, Davos, CH and Martell, IT) in 2015.

The data set contains for each site:

* 10 cm GIS raster of snow depth calculated from terrestrial laserscanning surveys (TLS) in the end of winter season (April/May)

* 10 cm GIS raster of snow depth calculated from TLS in the end of summer season (October)

Input files for SNOWPACK model:

* .sno: snow profile at the end of winter

* .smet: meteorological data measured by weather stations in the area

For more details see Grünewald, T., Lehning, M., and Wolfsperger, F.: Snow farming: Conserving snow over the summer season, The Cryosphere Discuss., https://doi.org/10.5194/tc-2017-93, in review, 2017.

snow

display_name: snow

snow-conservation

display_name: snow-conservation

snow-farming

display_name: snow-farming

snowpack

display_name: snowpack

terrestrial-laser-scanning

display_name: terrestrial-laser-scanning

winter-tourism

display_name: winter-tourism

education

en: Education and science

en: Snowfarming data set Davos and Martell 2015


maintainer: Jonas Stillhard

en: forest-structure carpathian-biosphere-reserve primeval-beech-forest permanent-forest-plot-data fagus-sylvatica dem stand-inventory-data forest-dynamics forest long-term-data-sets

en: Data description

en: Available online on publishers website

en: Dataset

en:
In 2000, a permanent forest plot of 10 ha has been established in the core zone of the primeval beech forest of Uholka.
 All living and dead trees with a diameter at breast height (DBH) ≥ 60 mm were identified to species, DBH measured, stems tagged and mapped.
Since then, the plot has been remeasured in 2005, 2010, and 2015. In total, 4,820 individual trees were measured with 14,116 individual measurements throughout all four inventories.
In spring 2018, an Airborne Laser Scan was carried out, covering the Uholka‐Shyrokyi Luh forest. This data set allows us to derive a high‐resolution digital elevation model (DEM) of the plot area.
The data set allows for important insights into the development and the spatial and temporal dynamics of primeval beech forests.

carpathian-biosphere-reserve

display_name: carpathian-biosphere-reserve

dem

display_name: dem

fagus-sylvatica

display_name: fagus-sylvatica

forest

display_name: forest

forest-dynamics

display_name: forest-dynamics

forest-structure

display_name: forest-structure

long-term-data-sets

display_name: long-term-data-sets

permanent-forest-plot-data

display_name: permanent-forest-plot-data

primeval-beech-forest

display_name: primeval-beech-forest

stand-inventory-data

display_name: stand-inventory-data

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Stand inventory data from the 10‐ha forest research plot in Uholka, Ukraine


maintainer: Frank Techel

en: danger-level avalanche-forecast snow european-alps warning-regions

en: shape file warning regions Alps

en: Forecast avalanche danger level

en: contains the highest forecast danger level (dangerLevelMax) for each day and warning region

en:
This dataset contains the data used in the publication by Techel et al., 2018 _Spatial consistency and bias in avalanche forecasts - a case study in the European Alps_ (Nat Haz Earth Syst Sci).
For details on the data please refer to this publication.
The dataset contains the following:
- shape files for the warning regions in the Alps
- highest forecast danger level for each warning region and day

avalanche-forecast

display_name: avalanche-forecast

danger-level

display_name: danger-level

european-alps

display_name: european-alps

warning-regions

display_name: warning-regions

en: Forecast avalanche danger level European Alps 2011 - 2015


irkis-soil-moisture-measurements-davos

maintainer: Nander Wever

title_for_slug: irkis-soil-moisture-measurements-davos

en: meteorological-measurements meteo-station dischma-catchment soil-moisture davos

en: IRKIS soil moisture data (zip file)

en: Compressed archive (zip) containing soil moisture measurements, as well as meteorological measurements and interpolated meteorological time series.

en:
Meteorological and soil moisture measurements from soil moisture stations installed from October 2010 - October 2013 in the area surrounding Davos, in particular in the Dischma catchment.

There are in total 7 stations: 1202, 1203, 1204, 1205, 222, 333 and SLF2. For each of the stations, there is a:

* vwc_[stn].smet: containing the soil moisture measurements

* station_[stn].smet: in-situ measured meteorlogical parameters. Note, the quality of these measurements for stations 1202, 1203, 1204 and 1205 is very low, with data gaps. Use this data with care. For stations 222, 333 and SLF2, data quality is high and only the default cautiousness should be applied.

* interpolatedmeteo_[stn].smet contains per stations a dataset derived by interpolating from several stations in the Davos area to the stations location. This dataset was generated from the output of the Alpine3D model, of which simulations are presented in the Wever et al. (2017) manuscript.

At the soil moisture measurement sites, Decagon 10HS sensors were installed, at 10, 30, 50, 80 and 120 cm depth. Per depth 2 sensors were installed, labelled A and B in the datafiles. Note that at stations 1203, 1204 and 1205, sensors were only installed at 10, 30 and 50 cm depth.

The files follow the SMET format: https://models.slf.ch/docserver/meteoio/SMET_specifications.pdf and metadata for the stations can be found in the header of the smet files.

Please cite the Wever et al. (2017) reference when using this data in publications.

For a more detailed description, please refer to: Wever, N., Comola, F., Bavay, M., and Lehning, M.: Simulating the influence of snow surface processes on soil moisture dynamics and streamflow generation in an alpine catchment, Hydrol. Earth Syst. Sci., 21, 4053-4071, https://doi.org/10.5194/hess-21-4053-2017, 2017.

davos

display_name: davos

dischma-catchment

display_name: dischma-catchment

meteo-station

display_name: meteo-station

meteorological-measurements

display_name: meteorological-measurements

soil-moisture

display_name: soil-moisture

en: IRKIS Soil moisture measurements Davos


en: biomass single-tree forest-inventory terrestiral-laserscanning tree-volume lidar

en: Individual tree parameters and volume estimates based on TLS point clouds and allometric models

en:
"ASM" denotes volume estimates calculated by allometric scaling models (as used in Swiss NFI), based on tree height, DBH and D7.
"QSM" denotes volume estimates derived from TLS point clouds by quantitative structure models (QSM).
The remaining variables are geometrical features directly calculated from single tree TLS point clouds.
For more detailed variable descriptions see related publication.

media_type: ZIP

en:
 Dataset
This dataset is based on terrestrial laser scanning (TLS) data acquired during winter 2020/2021 in leaf-off conditions, with a Leica BLK 360 instrument following a tree-centric scanning pattern.
The data was acquired on two sites (47.42°N 8.49°E and 47.504°N, 7.78°E), both of which were managed mixed temperate forest stands.
Individual trees were semi-automatically segmented from the co-registered TLS point clouds.

 Background
Accurate estimates of individual tree volume or biomass within forest inventories are essential for calibration and validation of biomass mapping products based on Earth observation data.
Terrestrial laser scanning (TLS) enables detailed and non-destructive volume estimation of individual trees, with existing approaches ranging from simple geometrical features to virtual 3D reconstruction of entire trees. Validating such approaches with weight measurements is a key step before the integration of TLS or other close-range technologies into operational applications such as forest inventories.
In this study, we firstly evaluate individual tree volume estimation approaches based on 3D reconstruction through quantitative structure models (QSM) against destructive reference data of 60 trees and compare them to operational allometric scaling models (ASM).
Secondly, we determine the explanatory power of TLS-derived geometric parameters regarding total tree, stem, coarse wood and fine branch volume.

biomass

display_name: biomass

forest-inventory

display_name: forest-inventory

lidar

display_name: lidar

single-tree

display_name: single-tree

terrestiral-laserscanning

display_name: terrestiral-laserscanning

tree-volume

display_name: tree-volume

education

en: Education and science

en: Individual tree TLS point clouds for tree volume estimation


en: sea-salt-aerosol arctic ice antarctica snow

en: Fig 1B_2

en: Individual data to mean shown in Figure 1B

en: Code

media_type: m

en: Matlab code to read and plot the data

en: This table lists all data files in chronological order. "SampleTemp" refers to the reading of the PT-1000 at the side of the sample holder. "cellPressure" is the water vapour pressure during the measurements. From this, in presence of ice, the temperature of the ice sample can be calculated which is given in the column "iceTemp". "dT" is the difference, or temperature offset, between sensor's reading and the ice samples temperature. The offset is due to the sensor being mounted away from the cooled spot of the sample were it experiences heating from warmer parts of the sample holder. The off-set is used to calibrate the temperature sensor and the resulting temperature during the experiments is given in the column "temperature". "relHum" is the relative humidity above (supercooled) liquid and "concBrine" is the concentration of a NaCl solution in mol/l in equilibrium with ice at the experimental temperature. "Area" is the integrated PE signal.

en: Fib 1B_3

en: Fig 1B_1

en: Fig 1C

en: Data to Figure 1C

en: Fig 1F_1

en: Individual data to mean shown in Figure 1F

en: Table 2

en: This table lists all NEXAFS data files in chronological order with PE carbon to oxygen signal intensity ratios -from which the atomic ratio were calculated in the paper - before ("preCtoO") and after ("postCtoO") each NEXAFS measurement. Also listed is the change in Na ("lossSodi"), O ("lossOxy"), Cl ("lossChlor"), and C ("lossCarb") signal intensity after the NEXAFS measurement compared to prior to the NEXAFS measurement.

en: Fig 1F_2

en: Read Me

en: Description of the data tables

en: Fig 1F

en: Data to Figure 1F

en: Fig 1D

en: Data to Figure 1D

en: Fig 1E

en: Data to Figure 1E

en: Fig 1B

en: Laboratory experiments are presented on the phase change at the surface of sodium chloride – water mixtures at temperatures between 259 K and 240 K. High selectivity to the upper few nanometres of the frozen solution – air interface is achieved by using electron yield near-edge X-ray absorption fine structure (NEXAFS) spectroscopy. We present the NEXAFS spectrum of the hydrohalite.

envidat

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Data set on interfacial supercooling and the precipitation of hydrohalite in frozen NaCl solutions by X-ray absorption spectroscopy


maintainer: Chunhong Zhao

en: land-dynamics urban-morphology land-use landscape austin

en: Land use dynamics in Austin since 1992

media_type: img

en: The present dataset is part of the published scientific paper Zhao C, Weng Q, Hersperger A M. Characterizing the 3-D urban morphology transformation to understand urban-form dynamics: a case study of Austin, Texas, USA. Landscape and urban planning, 2020, 203:103881. The overall objective of this paper is to understand urban form dynamics in the Austin metropolitan area for the periods 2006–2011 and 2011–2016. The study also aims to understand to what extent the changes in the built environment (in terms of ‘efficient growth’ versus ‘inefficient growth’) from the 1990s to 2016 in the Austin metropolitan area corresponded with ‘compact and efficient growth’ planning policy documents. The UMT distribution can be found in the paper. The area of transitioning UMT was provided in Table 2 and Table 3 can be found in the Appendix of the paper. A protocol was developed to perform the content analysis of the strategic plans and gather the data. The detailed list of protocol items can be found in Appendix B of the paper. This study demonstrates the advantage of applying Lidar data to characterize 3-D urban morphology type (UMT) transition and understand its dynamics, which helps develop a comprehensive understanding of the urbanization process and provides a tool for planning intentions and policies evaluation on urban development over time. The UMT maps can be found in Appendix A of the paper. The Lidar point datasets and the 30 × 30 m National Land Cover Database (NLCD) are the two main data sources of UMT mapping. Lidar datasets were gathered from different projects that had been conducted and collected by state agencies and other organizations between 2007 and 2017. Table A1 in the appendix in the paper shows the accuracies and acquisition parameters of the Lidar projects from 2007 to 2017. Land use/cover dynamics in Austin metropolitan area dataset provides Land use/cover patterns in the years 1992, 2001, 2004, 2006, 2008, 2011, 2013, 2016 with a spatial resolution of 30 meters. Since NLCD 1992 used a different classification system for the urban land classes, we first reclassified the NLCD 1992 using a customized Arcpy package.

austin

display_name: austin

land-dynamics

display_name: land-dynamics

land-use

display_name: land-use

landscape

display_name: landscape

urban-morphology

display_name: urban-morphology

education

en: Education and science

en: EnviDat: the Environmental Data Portal

en: Land use/cover dynamics in Austin metropolitan area since 1992


en: landscape place-attachment urban-ecology sense-of-place electrodermal-activity

en: 01. EDA Processed Data

en: 02. Questionnaire Data

en: 00. EDA Raw Data

en: 03. Publication Ready Data

en: 04. Scripts

en: 05. Visuals

en: This repository contains data on EDA measurements of visitors with different cultural backgrounds in virtual urban park settings. The parks are a Persian garden (Shiraz, Iran) and a historical park in Zurich, Switzerland. The cultural background of the visitors is Persian and Central European. The repository contains raw data from EDA, processed time series and statistical procedures.

electrodermal-activity

display_name: electrodermal-activity

place-attachment

display_name: place-attachment

sense-of-place

display_name: sense-of-place

urban-ecology

display_name: urban-ecology

en: Electrodermal Activity (EDA) of Bi-cultural Visitors In Virtual Park Settings


en: century air-temperature free-climate-data timeseries precipitation maximum-temperature minimum-temperature climate-layers climate

en: CHELSA Technical Documentation

media_type: application/pdf

en: As the CHELSA project is continuously expanded, certain new parameters, time scales etc. are not included anymore in the original publication (Karger et al. 2017). We are therefore provide this document as a guideline of the current climatic parameters available and will expand it as new parameters or time periods will become available. Please refer to the version history for changes made in the document.

en: Data Access

en:
CHELSAcruts is a delta change monthly climate dataset for the years 1901-2016 for mean monthly maximum temperatures, mean monthly minimum temperatures, and monthly precipitation sum. Here we use the delta change method by B-spline interpolation of anomalies (deltas) of the CRU TS 4.01 dataset. Anomalies were interpolated between all CRU TS grid cells and are then added (for temperature variables) or multiplied (in case of precipitation) to high resolution climate data from CHELSA V1.2 (Karger et al. 2017, Scientific Data). This method has the assumption that climate only varies on the scale of the coarser (CRU TS) dataset, and the spatial pattern (from CHELSA) is consistent over time. This is certainly a rather crude assumption, and for time periods for which more accurate data is available CHELSAcruts should be avoided if possible (e.g. use CHELSA V1.2 for 1979-2015). Different to CHELSA V1.2, CHELSAcruts does not take changing wind patterns, or temperature lapse rates into account, but rather expects them to be constant over time, and similar to the long term averages.

CHELSAcruts is licensed under a Creative Commons Attribution 2.0 Generic (CC BY 2.0) license.

air-temperature

display_name: air-temperature

century

display_name: century

climate

display_name: climate

climate-layers

display_name: climate-layers

free-climate-data

display_name: free-climate-data

maximum-temperature

display_name: maximum-temperature

minimum-temperature

display_name: minimum-temperature

precipitation

display_name: precipitation

timeseries

display_name: timeseries

envidat

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: CHELSAcruts -  High resolution temperature and precipitation timeseries for the 20th century and beyond


lwf-tea-bag-sites

maintainer: Marcus Schaub

title_for_slug: lwf-tea-bag-sites

en: roiboos litter green-tea decomposition forest tea-bag modeling verification carbon

en: Site Information

en: Descriptive information on sites

en:
Decomposition of plant litter is a key process for the transfer of carbon and nutrients in ecosystems. Carbon contained in the decaying biomass is released to the atmosphere as respired CO2, and may contribute to global warming. Litterbag studies have been used to improve our knowledge of the drivers of litter decomposition, but they lack comparability because litter quality is plant species-specific. The use of commercial tea bags as a standard substrate was suggested in order to harmonize studies, where green tea and rooibos represent more labile and more recalcitrant C compounds as surrogates of local litter.
The tea bag approach was implemented on eight sites of the Swiss long-term Forest Ecosystem Research (LWF) network (https://www.wsl.ch/LWF). This allowed us to take advantage from the existing infrastructure and data from a previous litterbag study with local litter. In Beatenberg and Schaenis, additional elevation transects were established (1200-1800 m and 540-1150 m, respectively) to examine particularly the effect of temperature on decomposition. In Pfynwald (https://www.wsl.ch/de/ueber-die-wsl/versuchsanlagen-und-labors/flaechen-im-wald/pfynwald.html) and Salgesch, infrastructure of running projects was used to examine the effect of drought and understory removal, respectively. In Novaggio, tea bags were incubated in summer and winter to study the effect of seasonality particularly precipitation. Tea bags are collected after 3, 12, 24, and 36 months; for the two time-shifted experiments additionally after 6 and 9 months.
The study has two primary objectives. Firstly, it contributes to TeaComposition initiative (http://teacomposition.org/) which aims at investigating long-term litter decomposition and its key drivers at present as well as under different future climate scenarios using a common protocol and standard litter (tea) across nine terrestrial biomes. Secondly, the data are used to further develop decomposition models such as Yasso (http://en.ilmatieteenlaitos.fi/yasso) which is used by several countries, including Switzerland to estimate the annual carbon fluxes in dead wood, litter, and soil for reporting in National Greenhouse Gas Inventories under the United Nations Framework Convention on Climate Change and the Kyoto Protocol.

carbon

display_name: carbon

decomposition

display_name: decomposition

forest

display_name: forest

green-tea

display_name: green-tea

litter

display_name: litter

modeling

display_name: modeling

roiboos

display_name: roiboos

tea-bag

display_name: tea-bag

verification

display_name: verification

education

en: Education and science

en: EnviDat: the Environmental Data Portal

en: LWF-Tea bag sites


en: hydrology precipitation alptal stable-water-isotope catchment stream-water

media_type: .txt

en:
Version 2 of the dataset contains additional information on:
- line-conditioned (lc) excess
- precipitation sample volumes
- isotope data quality

en: Single comma-separated txt-file with daily data of stable water isotopes measured in streamwater and precipitation in the Swiss Alptal catchment and two of its tributaries (Erlenbach and Vogelbach), as well as daily data of key hydrometeorological variables from the Alptal valley

en: Data Set Documentation: alptal_Isotopes_daily_2015-2019_v2

en: Documentations contains meta data of the data columns and information about the study catchments.

en: This dataset contains four years of daily measurements of the natural isotopic composition (2H, 18O) of precipitation and stream water at the Alp catchment (area 47 km2) in Central Switzerland and two of its tributaries (0.73 km2 and 1.55 km2). In addition, the dataset contains daily measurements of key hydrometeorological variables.

alptal

display_name: alptal

catchment

display_name: catchment

hydrology

display_name: hydrology

stable-water-isotope

display_name: stable-water-isotope

stream-water

display_name: stream-water

en: Four years of daily stable water isotope data in stream water and precipitation from three Swiss catchments


weissfluhjoch-dataset-for-esm-snowmip

title_for_slug: weissfluhjoch-dataset-for-esm-snowmip

en: snow-depth snow-models esm-snowmip snow weissfluhjoch

en: Dataset Weissfluhjoch for ESM-SnowMIP

en: ini file

en: This ini file is required by data-converter from MeteoIO version 1887 (http://models.slf.ch/p/meteoio/)

en: Conversion script

media_type: text/x-sh

en:
Use this script to convert WFJ_optimaldataset_v6.smet to wfj.smet, the file that is used for the ESM-SnowMIP project. It also requires the file: WFJ_AirPressure_Data.zip

Use: bash create_wfj_dataset.sh > input/wfj.smet

mimetype: text/x-sh

en: This Weissfluhjoch dataset is a processed version of the Weissfluhjoch dataset version 6 from https://doi.org/10.16904/6. This dataset was specially created for the ESM-SnowMIP project. Here it is documented how this dataset has been created.

esm-snowmip

display_name: esm-snowmip

snow

display_name: snow

snow-depth

display_name: snow-depth

snow-models

display_name: snow-models

weissfluhjoch

display_name: weissfluhjoch

en: Weissfluhjoch dataset for ESM-SnowMIP


en: failure-layer snow-profiles snowpack-simulation rutschblock snow-stratigraphy snowpack snow-instability

en: Simulated snow profiles

media_type: .csv

en: Simulated snow stratigraphy in form of one *.csv-file per snow profile from the DAV and SWISS data sets (in respective subfolders). Each file is named after the profile ID which allows identifying the corresponding observed profile in the "observed_profiles" folder. One row corresponds to one simulated snow layer. The content of the columns 1-7 is described in Table 3 of the ReadMe file. The remaining columns (8-31) contain all features that were used in the development of the RF model and are described in Table 1 of the ReadMe file.

en: Observed snow profiles

en: Observed snow stratigraphy in form of one *.csv-file per snow profile from the DAV and SWISS data sets (in respective subfolders). Each file is named after the profile ID which allows identifying the corresponding simulated profile in the "simulated_profiles" folder. One row corresponds to one observed snow layer. The content of the columns is described in Table 2 of the ReadMe file.

en: SWISS data set: Properties of observed snow profiles and simulated failure layers

en: For each profile pair from the SWISS data set, this file contains one row with information on the observed snow stability and all extracted features describing the simulated failure layers. Please note that only those profile pairs which fulfilled the similarity criteria (column similarity_obs_sim = 1, 121 profiles) were used to validate the RF model. The content of the columns is explained in Table 1 of the ReadMe file.

en: DAV data set: Properties of observed snow profiles and simulated failure layers

en:
For each profile pair from the DAV data set, this file contains one row with information on the observed snow stability and all extracted features describing the simulated failure layers. The content of the columns is explained in Table 1 of the ReadMe file. Only those profile pairs which fulfilled predefined similarity criteria (column
"similarity_obs_sim" = 1) and which belonged to the stability classes "stable" and "unstable" (column "stability_class"
= 0 or 1) were used to train the RF model. Please note that for each of the two snow profiles with RB score 7 (profile
IDs 7228 and 9526), three additional layers were included in this data set to obtain a balanced set of training data (73
unstable and 73 stable profiles).

en: ReadMe

en: ReadMe file explaining data structure and content

en:
This data set includes information on all observed and simulated snow profiles that were used to train and validate the
random forest model described in Mayer et al. (2022). The RF model was trained to assess snow instability from simulated snow stratigraphy. The data set contains observed snow profiles from the region of Davos (DAV subset, 512 profiles) and from all over Switzerland (SWISS subset, 230 profiles). For each observed snow profile, there is a corresponding simulated profile which was obtained using meteorological input data for the numerical snow cover model SNOWPACK. The information on the observed snow profile contains a Rutschblock test result including the depth of the failure interface. As part of the study described in Mayer et al. (2022), each observed snow profile was manually compared to its simulated counterpart and the simulated layer corresponding to the Rutschblock failure layer was identified.

The data are provided in the following form: one file each per observed and simulated snow profile (2x512 files DAV, 2x230 files SWISS), two files (1 file DAV, 1 file SWISS) containing the observed information on snow instability, the allocation between observed and simulated failure layer, and all features extracted from the simulated weak layers that were used to develop the RF model.

failure-layer

display_name: failure-layer

rutschblock

display_name: rutschblock

snow-instability

display_name: snow-instability

snow-profiles

display_name: snow-profiles

snow-stratigraphy

display_name: snow-stratigraphy

snowpack

display_name: snowpack

snowpack-simulation

display_name: snowpack-simulation

education

en: Education and science

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Observed and simulated snow profile data


restoring-grassland-multifunctionality

title_for_slug: restoring-grassland-multifunctionality

en: grassland ecosystem-multifunctionality nematodes restoration soil-biodiversity bacteria mineralization plants biodiversity fungi

en: Please note that C.sto and Corg have been corrected.

en: Functional diversity above_belowground fauna

en: Please note that EMF, MF.below, MF.abio and Soil.hetero have been corrected.

en:
Please cite this paper together with the citation for the datafile.

Resch, M. C., Schütz, M., Buchmann, N., Frey, B., Graf, U., van der Putten, W. H., Zimmermann, S., Risch, A. C. 2021. Evaluating long-term success in grassland restoration – an ecosystem multifunctionality approach. Ecological Applications 31, e02271.

 Study area

The study was conducted in the Canton of Zurich, Switzerland, in and around two nature reserves Eigental and Altläufe der Glatt (47°27’ to 47°29’ N, 8°37’ to 8°32’ E, 417 to 572 m a.s.l.). All studied grasslands were located with a radius of approximately 4 km. Average monthly temperatures range from 0.7 ± 2.0 °C (January) to 19.0 ± 1.5 °C (July), and monthly precipitation range from 60 ± 42 mm (January) to 118 ± 46 mm (July [maxima]; 1989-2017; MeteoSchweiz 2018). In our study, we focused on semi-dry and semi-wet oligo- to mesotrophic grasslands characterized by high plant species richness and groundwater fluctuations throughout the year (Delarze et al. 2015, see also Resch et al. 2019).

 Experimental design and sampling

A large-scale restoration experiment to expand and reconnect isolated remnants of species-rich grasslands was initiated in the nature reserve Eigental in 1990. Twenty hectares of adjacent intensive grasslands were chosen for restoration. In 1995, three restoration methods of increasing intervention intensities were implemented. The goal of all three methods was to lower the availability of soil nutrients and hence, facilitate ecosystem development towards the targeted nutrient-poor grasslands. These methods were: Harvest only (hay harvest twice a year), Topsoil (removal of the nutrient-rich topsoil), and Topsoil+Propagules (topsoil removal combined with the application of hay from target vegetation). Plant biomass harvest (once a year in late summer/early autumn) commenced in Topsoil and Topsoil+Propagules five years after the soils were removed and is still ongoing today. We measured restoration success by comparing the three restoration methods with intensively managed (Initial) and semi-natural grasslands (Target) 22 years after restoration. Initial grassland sites share the same agricultural history as the restored sites: mowing and subsequent fertilizing (manure) up to five times a year, as well as different tillage regimes (Resch et al. 2019). Target sites were the sites from which hay for seeding the Topsoil+Propagules sites was collected. Soil conditions (i.e., soil types, soil texture) were comparable to those found in the restored grasslands (Resch et al. 2019). Additionally, Target sites were selected to represent a variety of semi-natural grasslands, including semi-dry to semi-wet conditions. In Target grasslands, biomass is harvested once a year in late summer or early autumn. Eleven 5 m x 5 m (25 m2) plots were randomly established in each of the five treatments (in total 55 plots; for a detailed map see Neff et al. 2020). An additional 2 m x 2 m (4 m2) subplot was randomly established at least 2 m away from each 25 m2 plot for destructive sampling. Data sampling took place between June and September 2017.

Vegetation properties

All plant species were identified within the 25 m2 plots (nomenclature: Lauber and Wagner 1996) in mid-June 2017 (in total 250 species). Vegetation structure and plant biomass were assessed diagonally on a transect of 2 m x 10 cm within the 25 m2 plot in early July 2017. We measured the maximum and mean height of the vegetation at the start, middle and end of the transect and calculated the standard deviation of these measures to describe vegetation structural heterogeneity (Schuldt et al. 2019). Thereafter, biomass was clipped on the entire transect to 1 cm height, sorted into five functional groups (graminoids, forbs, legumes, litter, and woody species), dried at 60 °C for 48 h, and weighed (Meyer et al. 2015).

 Aboveground arthropods

Aboveground arthropods were sampled at two locations in each 25 m2 plot in early July 2017 (see also Neff et al. 2020). Briefly, two cylindrical baskets (50 cm diameter, 67 cm height; woven fabric) were thrown simultaneously from outside the plot into two opposite corners. A closable mosquito mesh sleeve was mounted to the top of the baskets and an integrated metal ring at the bottom was fixed to the ground with metal stakes to assure that insects could not escape. A suction sampler (Vortis, Burkhard Manufacturing Co. Ltd., Hertfordshire, England) was then inserted into one of the baskets through the opening of the sleeve and the plot was “vacuumed" twice for 105 seconds with a 30 seconds break. The collected animals were immediately transferred into 70% ethanol. Arthropods were sorted and assigned to 23 taxonomic groups. Holometabolic larvae were lumped into one category while hemimetabolic larvae were grouped separately from adults in the respective taxonomic rank. We used mean values of individuals per plot for total abundance. Aboveground arthropod richness was defined by the number of different taxa to lowest taxonomic level (in total 23 taxa). All taxa were assigned to one of five trophic levels: 1) primary producers, 2) primary consumers, 3) secondary consumers, 4) tertiary consumers, and 5) quaternary consumers.

 Belowground fauna

Sampling of all belowground fauna took place in mid-July 2017. Earthworms were sampled in two 30 cm x 30 cm x 20 cm soil monoliths at two opposite corners of the 25 m2 plot (opposite to aboveground arthropod sampling). The excavated soil monolith was broken by hand, all earthworms collected and immediately transferred in a 4% formaldehyde solution. Thereafter, earthworm individuals were identified to species level (in total 10 taxa; Christian and Zicsi 1999) and species assigned to three functional groups (Bouché 1977).
To assess soil arthropod communities, we randomly collected one undisturbed soil core (5 cm diameter, 12 cm depth) in each 4 m2 subplot with a slide hammer corer lined with a plastic sleeve (AMS Samplers, American Falls, Idaho, USA). Soil arthropods were extracted using Berlese-Tullgren funnels (3 mm mesh), starting the day of sampling and lasting 14 days. Individuals were stored in 70% ethanol. Soil arthropods were assigned to 41 taxonomic groups and 4 feeding types. Holometabolic and hemimetabolic larvae were treated as previously described for aboveground arthropods. Belowground arthropod richness refers to the 41 taxonomic groups.

For soil nematode sampling, we randomly collected eight soil cores of 2.2 cm diameter (Giddings Machine Company, Windsor, CO, USA) within each 4 m2 subplot to a depth of 12 cm. The eight cores were combined, gently homogenized, placed in coolers, kept at 4 °C and transported to the laboratory at NIOO in Wageningen (NL) within one week after collection. Free-living nematodes were extracted from 200 g of fresh soil using Oostenbrink elutriator (Oostenbrink 1960) and prepared for morphological identification and quantification as described by Resch et al. (2019). Nematodes were identified to family level (39 taxa) according to Bongers (1988), assigned to 17 functional groups, 5 feeding types and 5 colonizer-persister (C-P) classes (Yeates et al. 1993, Bongers 1990, Resch et al. 2019).

We randomly collected two more soil cores (2.2 cm diameter x 12 cm depth) within each 4 m2 subplot to determine soil microbial communities. Again, the soil cores were combined, homogenized, placed in coolers and transported to the laboratory at WSL in Birmensdorf (Switzerland) where the metagenomic DNA was extracted from 8 g sieved soil (2 mm) using the DNeasy PowerMax Soil Kit (Quiagen, Hilden, NRW, GER) according to the manufacturer`s instructions. PCR amplification of the V3-V4 region of the prokaryotic small-subunit (16S) and the ribosomal internal transcribed spacer region (ITS2) of eukaryotes was performed with 1 ng of template DNA utilizing PCR primers and conditions as previously described (Frey et al., 2016). PCRs were run in triplicates and pooled. The pooled amplicons were sent to the Genome Quebec Innovation Centre (Montreal, QC, Canada) for barcoding using the Fluidigm Access Array technology (Fluidigm) and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA). Quality filtering, clustering into operational taxonomic units (OTUs) and taxonomic assignment were performed as described by Frey et al. (2016) and Adamczyk et al. (2019). We used a customised pipeline largely based on UPARSE (Edgar 2013) implemented in USEARCH v. 9.2 (Edgar 2010). After discarding singletons of dereplicated sequences, clustering into OTUs with 97% sequence similarity was performed (Edgar 2013). Quality-filtered reads were mapped on the filtered set of centroid sequences. Taxonomic classification of prokaryotic and fungal sequences was conducted querying against most recent versions of SILVA (v.132, Quast et al. 2013) and UNITE (v.8, Nilsson et al. 2018). Only taxonomic assignments with confidence rankings equal or higher than 0.8 were accepted (assignments below 0.8 set to unclassified). Prokaryotic OTUs assigned to mitochondria or chloroplasts as well as eukaryotic OTUs assigned other than fungi were removed prior to data analysis. In addition, prokaryotic and fungal datasets were filtered to discard singletons and doubletons. Thereafter, OTU abundance matrices were rarefied to the lowest number of sequences per community, to normalize the total number of reads and achieve parity between samples (Prokaryota: 29,843 reads; Fungi: 26,690 reads). Finally, prokaryotic and fungal observed richness (number of OTUs) were estimated (Prokaryota: 14,010 OTUs; Fungi: 5,813 OTUs). For prokaryotes, we distinguished five and for fungi six functional types based on lowest taxonomic resolution (Nguyen et al. 2016, Tedersoo et al. 2014). Belowground taxon richness was defined by the total number of earthworm, arthropod, nematode, fungi, and prokaryote taxa assigned to lowest taxonomic level. Finally, all belowground taxa were assigned to the same five trophic levels as the aboveground arthropods.

 Soil chemical and physical properties, soil nitrogen mineralization

We randomly collected three 5 cm diameter x 12 cm depth soil samples in each 4 m2 subplot with a slide hammer corer (AMS Samplers, American Falls, Idaho, USA), pooled them and then made two subsamples. One was field-fresh and stored at 3 °C until analysis, the other was dried for 48 h at 60 °C and passed through a 4 mm mesh. From the dried sample, we measured soil pH potentiometrically in 0.01 M CaCl2 (soil:solution ratio=1:2; 30 minutes equilibration time). Total and organic carbon content were measured on fine-ground samples (≤ 0.5 mm) by dry combustion using a CN analyzer NC 2500 (CE Instruments, Wigan, United Kingdom). Inorganic carbon of samples with a pH > 6.5 was removed with acid vapor prior to analysis of organic carbon (Walthert et al. 2010). We calculated total soil carbon (C) storage after correcting its content for soil depth, stone content and density of fine earth (see below). Exchangeable cations were determined on another 5 g dry soil sample with 50 mL unbuffered 1 M NH4Cl solution (soil:solution ratio=1:10, end-over-end shaker for 1.5 hours) and measured by an ICP-OES (Optima 7300 DV, Perkin-Elmer, Waltham, Massachusetts, USA). Thereafter, cation exchange capacity (CEC) was calculated as the sum of exchangeable cations and protons (and expressed as mmolc per 1 kg soil) and used to describe nutrient retention capacity in our plots. Concentrations of exchangeable protons were calculated as the difference between total and Al-induced exchangeable acidity as determined by the KCl-method (Thomas 1982).

Ammonium (NH4+) and nitrate (NO3−) were extracted from a 20 g fresh subsample with 80 mL 1M KCl for 1.5 hours on an end-over-end shaker and filtered through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnemühle FineArt GmbH, Dassel, Germany). NH4+ concentrations were determined colorimetrically by automated flow injection analysis (FIAS 300, Perkin-Elmer, Waltham, Massachusetts, USA). NO3− concentrations were measured colorimetrically according to Norman and Stucki (1981). Potential soil net nitrogen (N) mineralization was assessed during an 8-week incubation period under controlled moisture (60% of field capacity), temperature (20 °C) and light conditions (dark) in the laboratory. We weighed duplicate samples of fresh soil equivalent to 8 g dry soil (24 h at 104 °C) into 50 mL Falcon tubes. Soil samples were extracted for NH4+ and NO3− at the beginning and after eight weeks as described above. Soil net N mineralization was calculated as the difference between the inorganic nitrogen (NH4+ and NO3−) before and after the incubation (Hart et al. 1994), corrected for the total incubation time and represented per day values expressed as mg N kg-1 soil d-1.

To assess soil physical properties, we randomly collected one undisturbed soil core per 4 m2 subplot (5 cm diameter, 12 cm depth) in a steel cylinder that fit into the slide hammer (AMS Samplers, American Falls, Idaho, USA). The cylinder was capped in the field to avoid disturbance. We then measured field capacity in the laboratory. For this purpose, the cylinder and soil therein were saturated in a water bath and drained on a sand/silt-bed with a suction corresponding to 60 cm hydrostatic head. The moist soil was dried at 105 °C to constant weight. Field capacity was calculated by dividing the mass of water by the total mass of wet soil contained at 60 cm hydrostatic head and used to describe water holding capacity. Thereafter, samples were passed through a 4 mm mesh. Fine-earth and skeleton fractions were weighed separately to assess bulk soil density (fine-earth plus skeleton), density of fine earth, and proportion of skeleton. Particle density was determined with the pycnometer method (Blake and Hartge 1986), and total porosity and proportion of fine pores were calculated (Danielson and Sutherland 1986). Clay, silt, and sand contents were quantified with the sediment method (Gee and Bauder 1986).

Surface and soil temperature (12 cm depth, water-resistant digital pocket thermometer; IP65, H-B Instrument, Trappe, Pennsylvania, USA) as well as volumetric soil moisture content (12 cm depth, time domain reflectometry; Field-Scout TDR 300, Spectrum Technologies, Aurora, Illinois, USA) were measured at five random locations within the 4 m2 subplots every month from June to September. We calculated the standard deviation of each temperature and moisture measure over four months to describe seasonal variations. Slope inclination was determined at plot-level via GPS measurements (GPS 1200, Leica Geosystem, Heerbrugg, Switzerland) and categorized into slope gradient classes according to FAO standards (1990). Thickness of the topsoil horizon (equivalent to Ah or Aa horizon) was determined at one soil monolith (30 x 30 x 30 cm3) per 4 m2 subplot in cm and rounded to next integer.

 References

Adamczyk, M., F. Hagedorn, S. Wipf, J. Donhauser, P. Vittoz, C. Rixen, A. Frossard, J. Theurillat, and B. Frey. 2019. The soil microbiome of GLORIA mountain summits in the Swiss Alps. Frontiers in Microbiology 10:1080-1101.

Blake, G.R., and K. H. Hartge. 1986. Particle Density. Pages 377-382 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Bongers, T. 1988. De nematoden van Nederland. Stichting Uitgeverij van de Koniklijke Nederlandse Natuurhistorische Verenigung (KNNV), Utrecht.

Bongers, T. 1990. The maturity index: an ecological measure of environmental disturbance based on nematode species composition. Oecologia 83:14-19.

Bouché, M. B. 1977. Strategies lombriciennes. Ecological Bulletins 25:122-132.

Christian, E., and A. Zicsi. 1999. Ein synoptischer Bestimmungsschlüssel der Regenwürmer Österreichs (Oligochaeta: Lumbricidae). Die Bodenkultur 50:121-131.

Danielson, R. E., and P. L. Sutherland. 1986. Porosity. Pages 443-461 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Delarze, R., Y. Gonseth, S. Eggenberg, and M. Vust. 2015. Lebensräume der Schweiz: Ökologie ‐ Gefährdung ‐ Kennarten, Ott Verlag, Bern.

Edgar, R. C. 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:2460–2461.

Edgar, R. C. 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods 10:996–998.

FAO. 1990. Guidelines for soil description, third ed. Land and Water Development Division at the Food and Agriculture Organization of the United Nations (FAO), Rome.

Frey, B., T. Rime, M. Phillips, B. Stierli, I. Hajdas, F. Widmer, and M. Hartmann. 2016. Microbial diversity in European alpine permafrost and active layers. FEMS Microbiology Ecology 92:fiw018.

Gee, G.W., and J. W. Bauder. 1986. Particle-size analysis. Pages 383-411 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Hart, S. C, J. M. Stark, E. A. Davidson, and M. K. Firestone. 1994. Nitrogen mineralization, immobilization, and nitrification. Pages 985-1016 in R. W. Weaver, S. Angle, P. Bottomley, D. Bezdicek, S. Smith, A. Tabatabai, and A. Wollum, editors. Methods of soil analysis: Part 2—Microbiological and biochemical properties. Soil Science Society of America (SSSA) Inc., Madison.

Lauber, K., Wagner, G., 1996. Flora Helvetica. Flora der Schweiz. Haupt Verlag, Bern.

MeteoSchweiz, 2018. Klimabulletin Jahr 2017. MeteoSchweiz, Zürich.

Meyer, S. T., C. Koch, and W. W. Weisser. 2015. Towards a standardized rapid ecosystem function assessment (REFA). Trends in Ecology and Evolution 30:390-397.

Neff, F., M. C. Resch, A. Marty, J. Rolley, M. Schütz, A. C. Risch, and M. M. Gossner. 2020. Long-term restoration success of insect herbivore communities in semi-natural grasslands – a functional approach. Ecological Applications 0:e02133

Nguyen, N.H., Z. W. Song, S. T. Bates, S. Branco, L. Tedersoo, J. Menke, J. S. Schilling, and P. G. Kennedy. 2016. FUNGuild: an open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology 20:241–248.

Nilsson, R. H., K.-H. Larsson, A. F. S. Taylor, J. Bengtsson-Palme, T. S. Jeppesen, D. Schigel, P. G. Kennedy, K. Picard, F. O. Glöckner, L. Tedersoo, I. Saar, U. Kõljalg, and K. Abarenkov. 2018. The UNITE database for molecular identification of fungi: handling dark taxa and parallel taxonomic classifications. Nucleic Acids Research 47:259–264.

Norman, R. J., and J. W. Stucki. 1981. The Determination of Nitrate and Nitrite in Soil Extracts by Ultraviolet Spectrophotometry. Soil Science Society of America Journal 45:347-353.

Oostenbrink, M. 1960. Estimating nematode populations by some selected methods. Pages 81-101 in J. J. Sasser, and W. R. Jenkins, editors. Nematology. Univ. of North Carolina Press, Chapel Hill.

Quast, C., E. Pruesse, P. Yilmaz, J. Gerken, T. Schweer, P. Yarza, J. Peplies, and F. O. Glöckner. 2013. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Research 41:590-596.

Resch, M. C., M. Schütz, U. Graf, R. Wagenaar, W.H. van der Putten, and A. C. Risch. 2019. Does topsoil removal in grassland restoration benefit both soil nematode and plant communities? Journal of Applied Ecology 56:1782-1793.

Schuldt, A., A. Ebeling, M. Kunz, M. Staab, C. Guimarães-Steinicke, D. Bachmann, N. Buchmann, W. Durka, A. Fichtner, F. Fornoff, W. Härdtle, L. R. Hertzog, A-N. Klein, C. Roscher, J. Schaller, von G. Oheimb, A. Weigelt, W. Weisser, C.Wirth, J. Zhang, H. Bruelheide, and N. Eisenhauer. 2019. Multiple plant diversity components drive consumer communities across ecosystems. Nature Communications 10:1460.

Tedersoo, L., M. Bahram, S. Põlme, U. Kõljalg, N. S. Yorou, R. Wijesundera, L. Villarreal Ruiz, A. M. Vasco-Palacios, P. Q. Thu, A. Suija, M. E. Smith, C. Sharp, E. Saluveer, A. Saitta, M. Rosas, T. Riit, D. Ratkowsky, K. Pritsch, K. Põldmaa, M. Piepenbring, C. Phosri, M. Peterson, K. Parts, K. Pärtel, E. Otsing, E. Nouhra, A. L. Njouonkou, R. H. Nilsson, L. N. Morgado, J. Mayor, T. W. May, L. Majuakim, D. J. Lodge, S. See Lee, K.-H. Larsson, P. Kohout, K. Hosaka, I. Hiiesalu, T. W. Henkel, H. Harend, L.-D. Guo, A. Greslebin, G. Grelet, J. Geml, G. Gates, W. Dunstan, C. Dunk, R. Frenkhan, L. Dearnaley, A. De Kesel, T. Dang, X. Chen, F. Buegger, F. Q. Brearley, G. Bonito, S. Anslan, S. Abell, and K. Abarenkov. 2014. Global diversity and geography of soil fungi. Science 346:1256688.

Thomas, G.W. 1982. Exchangeable cations. Pages 159-165 in A. L. Page, R. H. Miller, and D. R. Keeney, editors. Methods of Soil Analysis: Part 2—Chemical and microbiological properties. Soil Science Society of America (SSSA) Inc., Madison.

Walthert, L., U. Graf, A. Kammer, J. Luster, D. Pezzotta, S. Zimmermann, and F. Hagedorn. 2010. Determination of organic and inorganic carbon, δ13C, and nitrogen in soils containing carbonates after acid fumigation with HCl. Journal of Plant Nutrition and Soil Sciences 173:207-216.

Yeates, G. W., T. Bongers, R. G. M. de Goede, D. W. Freckman, and S. S. Georgieva. 1993. Feeding habits in soil nematode families and genera – an outline for soil ecologists. Journal of Nematology 25:315-331.

bacteria

display_name: bacteria

biodiversity

display_name: biodiversity

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

fungi

display_name: fungi

grassland

display_name: grassland

mineralization

display_name: mineralization

nematodes

display_name: nematodes

plants

display_name: plants

restoration

display_name: restoration

soil-biodiversity

display_name: soil-biodiversity

en: Restoring grassland multifunctionality