vegetation-height-model-nfi

maintainer: Christian Ginzler

title_for_slug: vegetation-height-model-nfi

en: vegetation-height-model 3d point-cloud forest photogrammety

en: 2019 Vegetation Height Model NFI (current)

en:
Data 'Vegetation Height Model NFI 2019' available on request (christian.ginzler@wsl.ch).

A national vegetation height model was calculated for Switzerland using digital aerial images. We used the stereo aerial images acquired by the Federal Office of Topography swisstopo using the ADS80 sensor to first calculate a digital surface model (DSM) with a very high spatial resolution (1 × 1 m). The DSM was then normalized to obtain the actual vegetation heights using the digital terrain model (DTM) SwissAlti3D based on airborne laser data. Buildings are masked out using the NDVI information of the aerial images to produce a vegetation height model, and to produce a vegetation height model (VHM). Such a model will be calculated in the framework of the Swiss National Forest Inventory (NFI) with consistent methods and a very high level of detail. For covering the whole of Switzerland, we used summer aerial images from 2013 to 2019.

en: Thumbnail

en: 2012 Vegetation Height Model NFI

en:
Data 'Vegetation Height Model NFI 2012' available on request (christian.ginzler@wsl.ch).

A national vegetation height model was calculated for Switzerland using digital aerial images. We used the stereo aerial images acquired by the Federal Office of Topography swisstopo using the ADS80 sensor to first calculate a digital surface model (DSM) with a very high spatial resolution (1 × 1 m). The DSM was then normalized to obtain the actual vegetation heights using the digital terrain model (DTM) SwissAlti3D based on airborne laser data. Buildings are masked out using the NDVI information of the aerial images to produce a vegetation height model, and to produce a vegetation height model (VHM). Such a model will be calculated in the framework of the Swiss National Forest Inventory (NFI) with consistent methods and a very high level of detail. For covering the whole of Switzerland, we used summer aerial images from 2007 to 2012.

en: 2016 Vegetation Height Model NFI

en:
Data 'Vegetation Height Model NFI 2016' available on request (christian.ginzler@wsl.ch).

A national vegetation height model was calculated for Switzerland using digital aerial images. We used the stereo aerial images acquired by the Federal Office of Topography swisstopo using the ADS80 sensor to first calculate a digital surface model (DSM) with a very high spatial resolution (1 × 1 m). The DSM was then normalized to obtain the actual vegetation heights using the digital terrain model (DTM) SwissAlti3D based on airborne laser data. Buildings are masked out using the NDVI information of the aerial images to produce a vegetation height model, and to produce a vegetation height model (VHM). Such a model will be calculated in the framework of the Swiss National Forest Inventory (NFI) with consistent methods and a very high level of detail. For covering the whole of Switzerland, we used summer aerial images from 2011 to 2016.

en: 2015 Vegetation Height Model NFI

en:
Data 'Vegetation Height Model NFI 2015' available on request (christian.ginzler@wsl.ch).

A national vegetation height model was calculated for Switzerland using digital aerial images. We used the stereo aerial images acquired by the Federal Office of Topography swisstopo using the ADS80 sensor to first calculate a digital surface model (DSM) with a very high spatial resolution (1 × 1 m). The DSM was then normalized to obtain the actual vegetation heights using the digital terrain model (DTM) SwissAlti3D based on airborne laser data. Buildings are masked out using the NDVI information of the aerial images to produce a vegetation height model, and to produce a vegetation height model (VHM). Such a model will be calculated in the framework of the Swiss National Forest Inventory (NFI) with consistent methods and a very high level of detail. For covering the whole of Switzerland, we used summer aerial images from 2010 to 2015.

en: Countrywide Stereo-Image Matching for Updating Digital Surface Models in the Framework of the Swiss National Forest Inventory

en:
A national vegetation height model was calculated for Switzerland using digital aerial images.
We used the stereo aerial images acquired by the Federal Office of Topography swisstopo using the ADS80 sensor to first calculate a digital surface model (DSM) with a very high spatial resolution
(1 × 1 m and 0.5 x 0.5 m). The DSM was then normalized to obtain the actual
vegetation heights using a digital terrain model (DTM)
based on laser data with the buildings masked out, and to
produce a vegetation height model (VHM). Such a model
will be calculated in the framework of the Swiss National Forest Inventory (NFI) with consistent methods and a very
high level of detail. For covering the whole of Switzerland,
we use summer aerial images from six years.

Latest version is from 2019.

forest

display_name: forest

photogrammety

display_name: photogrammety

point-cloud

display_name: point-cloud

vegetation-height-model

display_name: vegetation-height-model

en: Vegetation Height Model NFI


aerosol-data-weissfluhjoch

maintainer: EnviDat Support

title_for_slug: aerosol-data-weissfluhjoch

en: raclets aerosol ice-nucleation inp particles linda

state: active

type: dataset

en: OPC-N3 Dataset Weissfluhjoch

media_type: text/csv

en: Particle concentrations in a size range of 0.35 μm to 40 μm were measured with an OPC-N3 (Alphasense, Inc.) in a resolution of 20 seconds (time in UTC). PM₁, PM₂,₅ and PM₁₀ were directly derived.

format: CSV

mimetype: text/csv

en: LINDA Dataset Weissfluhjoch

en: The LINDA file contains information on temperature (in °C) dependent frozen fractions, number of Ice Nuleating Particles (INP, per standard liter) and differential INP concentrations (per standard liter and Kelvin).

format: SERVICE

en: OPC-GT526S Dataset Weissfluhjoch

en: Particle counts were recorded in six size channels (0.3, 0.5, 0.8, 1.5, 2.5 and 5 μm) in a resolution of 20 seconds (time in UTC) by a particle counter (GT-526S, Met One Instruments, Inc.).

en: APS Dataset Weissfluhjoch

en: The APS file contains aerosol data (total number concentration, median, mean, geometric mean, mode and geometric standard deviation of the size distribution) for aerodynamic diameters between 0.5 μm and ~20 μm as well as instrument characteristics, in a temporal resolution of 20 seconds, with time in UTC, measured at Weissfluhjoch.

mimetype: application/x-netcdf

en: SMPS Dataset Weissfluhjoch

en: The SMPS file contains aerosol data (particle concentration, total number concentration, median, mean, geometric mean, mode and geometric standard deviation of the size distribution) in a size range between 12 and 460 nm (electrical mobility diameter) as well as instrument characteristics (sample temperature, pressure, relative humidity, mean free path and gas viscosity) in a temporal resolution of 2 minutes, with time in UTC, measured at Weissfluhjoch.

en:
Aerosol properties were measured between February 8 and March 31 2019 at the measurement site Weissfluhjoch (LON: 9.806475, LAT: 46.832964). Optical and aerodynamic particle counters, as well as a scanning mobility particle size spectrometer and an ice nuclei counter were deployed to report particle concentrations and size distributions in fine (10-1000 nm) and coarse mode (> 1000 nm), cloud condensation nuclei concentrations (CCNCs), and ice nuclei particle concentrations (ICNCs). The ambient particles were transported via a heated inlet to be distributed to the particle detecting devices inside the setup room.

Optical Particle Counter (OPC): Light scattering of a diode laser beam caused by travelling particles is used in the both, the OPC-N3 (0.41 - 38.5 μm) and GT-526S (0.3 – 5 μm), to determine their size and number concentration. For the OPC-N3, particle size spectra and concentration data are used afterwards to calculate PM₁, PM₂,₅ and PM₁₀ (assumptions: particle density: 1.65 g cmˉ³, refractive index: 1.5+i0).

Aerodynamic Particle Sizer (APS): The APS (3321, TSI Inc.) measured the particle size distribution for aerodynamic diameters between 0.5 μm and ~20 μm by the particle’s time-of-flight and light-scattering intensity (assumptions: particle density 1 g cmˉ³).

Scanning Mobility Particle Size Spectrometer (SMPS): Particle number concentrations in a size range between 12 and 460 nm (electrical mobility diameter) were measured at Davos Wolfgang, using a Scanning Mobility Particle Sizer Spectrometer (SMPS 3938, TSI Inc.). The classifier (3082, TSI Inc.) was equipped with a neutralizer (3088, TSI Inc.) and a differential mobility analyzer working with negative polarity (3081, TSI Inc.). The size selected particles were counted by a water-based condensation particle counter (3787 TSI Inc.). The TSI AIM software was used to provide particle size distributions by applying multiple charge and diffusion loss corrections (assumptions: particle density 1 g cmˉ³).

Coriolis μ and LINDA: A microbial air sampler (Coriolis μ, bertin Instruments) was used to collect airborne particles for investigating their ice nucleating ability with a droplet freezing device. Particles larger than 0.5 μm were drawn with an air flow rate of up to 300 l min‾¹ into the cone and centrifuged into the wall of the cone due to the forming vortex. The liquid sample was transferred into the LED based Ice Nucleation Detection Apparatus (LINDA, University of Basel) to study heterogeneous ice formation (immersion freezing mode) of ambient airborne particles.

aerosol

display_name: aerosol

ice-nucleation

display_name: ice-nucleation

inp

display_name: inp

linda

display_name: linda

particles

display_name: particles

raclets

display_name: raclets

educ

fr: Éducation, culture et sport

de: Bildung, Kultur und Sport

en: Education, culture and sport

it: Istruzione, cultura e sport

envidat

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

en: EnviDat: the Environmental Data Portal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: Aerosol Data Weissfluhjoch


maintainer: Anita Risch

en: grazing ecosystem-multifunctionality ecosystem-coupling swiss-national-park invertebrates vertebrates grassland ecosystem-functions exclusion subalpine

en: Datafile

en:
ID: Plot ID composited of Fence & Treatment number (90 plots total)

Grassland: Name of the six subalpine grasslands where the exclosure setups were erected. In large grasslands (GRIM, MING, STAB), we established four hierarchical exclosure setups (two in short- and two in tall-grass vegetation), whereas in the small grasslands (BOTS, DADA, MARG) only one setup was erected per vegetation type. BOTS = Val dal Botsch, GRIM = Grimmels, MING = Val Mingér, STAB = Alp Stabelchod, DADA = Stabelchod da daint, MARG = Margunet

Fence: Number of exclosure setup (1 - 18). Note that uneven numbers (1,3,5,7,9,11,13,15,17) represent setups established on short-grass vegetation, even numbers (2,4,6,8,10,12,14,16,18) setups established in tall-grass vegetation
Vegetation	Vegetation type, 1 = short-grass vegetation, 2 = tall-grass vegetation; (9 replicate fences on each vegetation type)
Treatment	Progressive animal exclusion, numbers indicate which animals had access to the plot: 1 = Large/medium/small mammals, and invertebrates have access ("L/M/S/I"), 2 = Medium/small mammals, and invertebrates have access (“M/S/I”), 3 = Small mammals and invertebrates have access (“S/I”), 4 = Invertebrates have access (“I”), 5 = No animals have access (“None”)

Bulkden:	Fine fraction soil bulk density (g cm-3)

Soil.moist	: Soil moisture (%)

Soil.org.C: Rhizosphere soil C content (%)

pH: Soil pH

Nema.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil nematode community composition

Nema.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil nematode community composition

Micro.PC1: 	First principal component (PC1) of principal component analysis (PCA) of the soil microbial community composition

Micro.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil microbial community composition

Abv.Inv.PC1: First principal component (PC1) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Abv.Inv.PC2: Second principal component (PC2) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Arthr.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil arthropod community composition

Arthr.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil arthropod community composition

Plant.PC1: First principal component (PC1) of principal component analysis (PCA) of the plant community composition

Plant.PC2: Second principal component (PC2) of principal component analysis (PCA) of the plant community composition

Root.biomass: Root biomass (g m-2)

Micro.biomass	: Microbial biomass carbon (mg kg-1)

Plant.richness: Plant species richness

Plant.N: Plant tissue nitrogen content (%)

Soil.N.min: Soil net nitrogen mineralisation (kg ha-1 season)

Soil.resp: Soil carbon dioxide emissions (soil respiration; g m-2 h-1)

format: XLS

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:
 > Risch AC, Ochoa-Hueso R, van der Putten WH, Bump JK, Busse MD, Frey B, Gwiazdowicz DJ, Page-Dumroese DS, Vandegehuchte ML, Zimmermann S, Schütz M. Size-dependent loss of aboveground animals differentially affects grassland ecosystem coupling and functions. 2018. Nature Communications 9: 3684. [doi: 10.1038/s41467-018-06105-4](https://doi.org/10.1038/s41467-018-06105-4).

Please cite this paper together with the citation for the datafile.

#Methods
Study sites
The experimental exclosure setups were installed within the SNP (IUCN category Ia preserve; Dudley 2008), in south-eastern Switzerland. The park covers 172 km2 of forests and subalpine and alpine grasslands along with scattered rock outcrops and scree slopes. The entire area has been protected from human impact (no hunting, fishing, camping or off-trail hiking) since 1914. Large, fairly homogenous patches of short- and tall-grass vegetation, which originate from different historical management and grazing regimes, cover the park’s subalpine grasslands entirely. Short-grass vegetation developed in areas where cattle used to rest (nutrient input) prior to the park’s foundation (14th century to 1914) (Schütz and others 2003, 2006) and is dominated by lawn grass species such as Festuca rubra L., Briza media L. and Agrostis capillaris L. (Schütz and others 2003, 2006). Today, this vegetation type is intensively grazed by diverse vertebrate and invertebrate communities that inhabit the park and consume up to 60% of the available biomass (Risch and others 2013). Tall-grass vegetation developed where cattle formerly grazed, but did not rest, and is dominated by rather nutrient-poor tussocks of Carex sempervirens Vill. and Nardus stricta L. (Schütz and others 2003, 2006). This vegetation type receives considerably less grazing, with only roughly 20% of the biomass consumed (Risch and others 2013). Consequently, the two vegetation types together represent a long-term trajectory of changes in grazing regimes. Underlying bedrock of all grasslands is dolomite, which renders these grasslands rather poor in nutrients regardless of former and current land-use regimes.
Experimental design
To progressively exclude aboveground vertebrate and invertebrate animals, we established 18 size-selective exclosure setups (nine in short-grass, nine in tall-grass vegetation) distributed over six subalpine grasslands across the SNP (Risch and others 2013, 2015). Elevation differences of exclosure locations did not exceed 350 m (between 1975 and 2300 m a.s.l.). The exclosures were established immediately after snowmelt in spring 2009 and were left in place for five consecutive growing seasons (until end of 2013). They were, however, temporarily dismantled every fall (late October after first snowfall) to protect them from avalanches. They were re-established in the same location every spring immediately after snowmelt. Each size-selective exclosure setup consisted of five plots (2 x 3 m) that progressively excluded aboveground vertebrates and invertebrates from large to small. The plots are labelled according to the guilds that had access to them “L/M/S/I”, “M/S/I”, “S/I”, “I”, “None”; L = large mammals, M = medium mammals, S = small mammals, I = invertebrates, None = no animals had access. As we only had permission to have the experimental setup in place for five consecutive growing seasons, the experiment had to be completely dismantled in the late fall of 2013 and all material removed from the SNP.
Our exclosure design was aimed at excluding mammalian herbivores, but naturally also excluded the few medium and small mammalian predators, as well as the entire aboveground invertebrate food web. A total of 26 large to small mammal species can be found in the SNP, but large apex predators are missing (wolf, bear, lynx) . Reptiles, amphibians and birds are scarce to absent in the subalpine grasslands under study. Only two reptile species occur in the park and they are confined to rocky areas that warm up enough for them to survive. One frog species spawns in an isolated pond far from our grasslands. Only three bird species occasionally feed on the subalpine grasslands. Using game cameras (Moultrie 6MP Game Spy I-60 Infrared Digital Game Camera, Moultrie Feeders, Alabaster, AL, USA), we did observe that the medium- and small-sized mammals (marmot/hares and mice) were not afraid to enter the fences and feed on their designated plots. We never spotted reptiles, amphibians or birds on camera. We distinguished between 59 higher aboveground-dwelling invertebrate taxa that our size-selective exclosures excluded (see also methods for aboveground-dwelling invertebrates below).
The “L/M/S/I” plot (not fenced) was located at least 5 m from the 2.1 m tall and 7 x 9 m large main electrical fence that enclosed the other four plots. The bottom wire of this fence was mounted at 0.5 m height and was not electrified to enable safe access for medium and small mammals, while fencing out the large ones. Within each main fence, we randomly established four 2 x 3 m plots separated by 1-m wide walkways from one another and from the main fence line: 1) the “M/S/I” plots were unfenced, allowing access to all but the large mammals; 2) the “S/I” plots (10 x 10 cm electrical mesh fence) excluded all medium-sized mammals. Note that the bottom 10 cm of this fence remained non-electrified to enable safe access for small mammals; 3) the “I” plots (2 x 2 cm metal mesh fence) excluded all mammals. We double-folded the mesh at the bottom 50 cm to reduce the mesh size to smaller than 1 x 1 cm openings; and 4) the “None” plots were surrounded by a 1 m tall mosquito net (1.5 x 2 mm) to exclude all animals. The top of the plot was covered with a mosquito-meshed wooden frame mounted to the corner posts (roof). We treated these plots a few times with biocompatible insecticide (Clean kill original, Eco Belle GmbH, Waldshut-Tiengen, Germany) to remove insects that might have entered during data collection or that hatched from the soil, but amounts were negligible and did not impact soil moisture conditions within these plots.
To assess whether the design of the “None” exclosure (mesh and roof) affected the response variables within the plots and, therefore, influenced the results, we established an additional six “micro-climate control” exclosures (one in each of the six grasslands) (Risch and others 2013, 2015). These exclosures were built as the “None” exclosures but were open at the bottom (20 cm) of the 3-m side of the fence facing away from the prevailing wind direction to allow invertebrates to enter. A 20-cm high and 3-m long strip of metal mesh was used to block access to small mammals. Thus, this construction allowed a comparable micro-climate to the “None” plots, but also a comparable feeding pressure by invertebrates to the “I” plots. We compared various properties within these exclosures against one another to assess if our construction altered the conditions in the “None” plots. We showed that differences in plant (e.g., vegetation height, aboveground biomass) and soil properties (e.g., soil temperature, moisture) found between the “I” and the “None” treatments were not due to the construction of the “None” exclosure, but a function of animal exclusions, although the amount of UV light reaching the plant canopy was significantly reduced (Risch and others 2013).
Aboveground invertebrate sampling
Aboveground invertebrates were sampled with two different methods to capture both ground- and plant-dwelling organisms: 1) we randomly placed two pitfall traps (67 mm in diameter, covered with a roof) filled with 20% propylene glycol in one 1 x 1 m subplot of the 2 x 3 m treatment plots in spring 2013 (May) and emptied them every two weeks until late September 2013 (Vandegehuchte and others 2017b, 2018). A pitfall trap consisted of a plastic cylinder (13 cm depth, 6.75 cm diameter). Within each cylinder we placed a 100 ml plastic vial with outer diameter 6.70 cm and on top of the cylinder we placed a plastic funnel to guide the invertebrates into the vials. Each trap was cover with a cone-shaped and transparent plastic roof to protect the trap from rain (Vandegehuchte and others 2017b, 2018). Note that in the “None” plots only one trap was placed as control to check for effectiveness of the exclosure. 2) We vacuumed all invertebrates from a 60 x 60 cm area on another 1 x 1 m subplot with a suction sampler (Vortis, Burkhard manufacturing CO, Ltd., Rickmansworth, Hertfordshire, UK) every month from June to September 2013 (Vandegehuchte and others 2017b, 2018). For this purpose, we quickly placed a square plastic frame (60 x 60 x 40 cm) with a closable mosquito mesh sleeve attached to the top edge into the plot from the outside. The suction sample was then inserted into through the sleeve and operated for 45 s to collect the invertebrates (Vandegehuchte and others 2017b, 2018).
We sorted the ≈100 000 individuals collected with both methods by hand and identified each individual morphologically to the lowest taxonomic level feasible (59 taxa, including orders, suborders, subfamilies, families; phylum for Mollusca). These taxa belonged to the following feeding types: 19 herbivores, 16 detritivores, 9 predators, 8 mixed feeders, 5 omnivores and 2 non-classified feeders (or not feeding as adults) (Vandegehuchte and others 2017b). We summed the numbers from the two pitfall traps and the suction sampling over the course of the 2013 season to represent the aboveground invertebrate abundance and community composition of a plot. Note: we did not specifically attempt to catch flying invertebrates with e.g., sticky traps, thus a few flying insects may have been missed with our vacuum sampling approach.
Sampling of plant properties
The vascular plant species composition was assessed at peak biomass every summer (July) by estimating the frequency of occurrence of each species with the pin count method in each plot (Frank and McNaughton 1990). A total of 172 taxa occurred within our 90 plots and we calculated plant species richness for each plot separately. We used the 2013 data in this study. Plant quality was assessed every year in July and September; here we use plant quality at the end of the experiment (September 2013). Two 10 x 100 cm wide strips of vegetation per plot were clipped, combined, dried at 65°C, and ground (Pulverisette 16, Fritsch, Idar-Oberstein, Germany) to pass through a 0.5 mm sieve. Twenty randomly selected samples across all treatments were analysed for N (Leco TruSpec Analyser, Leco, St. Joseph, Michigan, USA) (Vandegehuchte and others 2015). Nitrogen concentrations of the other samples were then estimated from models established for the experiment and the entire SNP relating Fourier transform-near infrared reflectance (FT-NIR) spectra to the measured values of N using a multi-purpose FT-NIR spectrometer (Bruker Optics, Fällanden, Switzerland) (Vandegehuchte and others 2015). Root biomass was sampled every fall by collecting five 2.2 cm diameter x 10 cm deep soil samples (Giddings Machine Company, Windsor, CO, USA) per plot (450 samples year-1). The samples were dried at 30 °C and roots were sorted from the sample by hand. We sorted each sample for 1 h which allowed to retrieve over 90% of all roots present in the samples (Risch and others 2013). The roots were then dried at 65 °C for 48 and weighed to the nearest mg. We averaged the values per plot and used the 2013 data only in this study.
Sampling of edaphic communities
In 2009, 2010, and 2011 we collected three composited soil samples (5 cm diameter x 10 cm depth; AMS Samplers, American Falls, ID, USA) and assessed bacterial community structure using T-RFLP profiling (Liu and others 1997; Blackwood and others 2003; Hodel and others 2014). We detected a total of 89 operational taxonomic units (OTUs). These values are in accordance with other studies reporting OTU richness (Wirthner and others 2011; Zumsteg and others 2012; Meola and others 2014) using T-RFLP profiling, a method that detects the most abundant, and thus likely, the most relevant, taxa. We averaged the data over the three years of collections for our calculations. Microbial biomass carbon (MBC) was determined with the substrate-induced method (Anderson and Domsch 1978) every fall (September) between 2009 and 2013 by collecting three mineral soil samples (5 cm diameter × 10 cm mineral soil core, AMS Samplers, American Falls, ID, USA). The three samples were combined (90 samples for each sampling year), immediately put on ice, taken to the laboratory, passed through a 2-mm sieve and stored at 4°C. Again, we only used the 2013 data in this study.
Soil samples (5 cm diameter x 10 cm depth) to extract soil arthropods were collected in June, July, and August 2011 with a soil corer lined with a plastic sleeve to ensure an undisturbed sample (total of 270 samples). The plastic line core was immediately sealed on both ends using cling film and put into a cooler. All plots were sampled within three days and the extraction of arthropods started the evening of the sampling day using a high-gradient Tullgren funnel apparatus (Crossley and Blair 1991; Vandegehuchte and others 2015). Samples were kept in the extractor for four days and the soil arthropods were collected in 95% ethanol. All individuals were counted and each individual was identified morphologically to the lowest level feasible [76 taxa, including orders, suborders, subfamilies, families (Protura, Thysanoptera, Aphidina, Psylina, Coleoptera, Brachycera, Nematocera, Auchenorryncha, Heteroptera, Formicidae); sub-phylum for Myriapoda, for Acari and Collembola also including morpho-species). Note that we also included larval stages (nine of the 76 taxa) (Vandegehuchte and others 2015). All data were summed over the season. A detailed species list for mites and collembolans is published (Vandegehuchte and others 2017a) [https://doi.org/10.1371/journal.pone.0118679.s001]. Earthworms are rare in the SNP and therefore were not included. We collected eight random 2.2 cm diameter x 10 cm deep soil cores from each plot in September 2013 to determine the soil nematode community composition. The samples were mixed and the nematodes were extracted from 100 ml of fresh soil using Oostenbrink elutriators (Oostenbrink 1960). All nematodes in a 1 ml of the 10 ml extract were counted, a minimum of 150 individuals sample-1 were identified to genus or family level using (Bongers 1988), the numbers of all nematodes were extrapolated to the entire sample and expressed for a 100 g dry sample. In total we identified 63 genus or family levels (Vandegehuchte and others 2015). The list of all the nematodes found is published (Vandegehuchte and others 2015) [http://www.oikosjournal.org/appendix/oik-03341] or DOI: [doi: 10.1111/oik.03341].
We are aware that sampling soil microbes from 2009 to 2011 and soil arthropods in 2011 was not ideal, but we are positive that this does not bias the results. Most of the parameters measured in our experiment either already showed a treatment response after the first growing season (e.g., plant biomass) or did not respond over the entire time experiment (e.g., microbial biomass C). The microbial community composition (2009 – 2011) was highly influenced by inter-annual differences in temperature and precipitation, but did not differ between treatments or vegetation types (Hodel and others 2014). We therefore felt comfortable using the 2009 through 2011 data for describing the soil microbial community in our experimental treatments. Similarly, we are positive that our soil arthropod data are representative. We did assess soil arthropods in August 2012 and found no differences to the August 2011 data. However, we did not feel comfortable combining the 2011 June, July, August data with only August data for 2012 for our analyses.
Sampling of soil properties
We collected three soil samples (5 cm diameter x 10 cm depth) in each plot in September 2013 after removing the vegetation. First, we collected the top layer of mineral soil rich in organic matter, the surface organic layer or rhizosphere, typically 1 to 3 cm in depth with a soil corer (AMS Samples, American Falls, Idaho, USA). Second, we collected a 10 cm mineral soil core beneath this surface layer. The cores for each layer were composited, dried at 65 °C for 48 h and fine-ground to pass a 0.5 mm screen. We then analysed all samples for total C using a Leco TruSpec Analyser (Leco, St. Joseph, Michigan, USA). Mineral soil pH was measured potentiometrically in 1:2 soil:CaCl2 solution with an equilibration time of 30 min.
Soil net N mineralisation was assessed during the 2013 growing season (Risch and others 2015). For this purpose, we randomly collected a 5 cm diameter x10 cm deep soil sample with a soil corer (AMS Samples, American Falls, Idaho, USA) after clipping the vegetation in June 2013. After weighing and sieving (4 mm mesh) the soil, we extracted a 20 g subsample in 1 mol l-1 KCl for 1.5 h on an end-over-end shaker and thereafter filtered it through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnenmühle FineArt GmbH, Dassel, Germany). From these filtrates NO3- concentrations were measured colorimetrically (Norman and Stucki 1981) and NH4+with flow injection analysis (FIAS 300, Perkin Elmer, Waltham Massachusetts, USA) (Risch and others 2015). We dried the rest of the sample 105 °C to constant mass to determine fine,fraction bulk density. A second soil sample was collected within each plot in June 2013 with a corer lined with a 5 x 13 cm aluminium cylinder. The corer was driven 11.5 cm deep into the soil so that the top 1.5 cm of the cylinder remained empty. Into this space we placed a polyester bag (250 µm) filled an ion-exchanger resin to capture the incoming N. The bag was filled with a 1:1 mixture of acidic and alkaline exchanger resin (ion-exchanger I KA/ion exchanger IIIAA, Merck AG, Darmstadt, Germany). We then removed 1.5 cm soil at the bottom of the cylinder and placed a second resin exchanger bag into this space to capture the N leached from the soil column. To assure that the exchange resin was saturated with H+ and Cl- prior to filling the bags, the mixture was stirred with 1.2 ml l-1 HCl for 1 h and then rinsed with demineralized water until the electrical conductivity of the water reached 5 µm cm-1. The cylinder with the resin bags in place was reinserted into the soil with the top flush to the soil surface and incubated for three months. We recollected the cylinders in September 2013. Each resin bag and 20 g of sieved soil (4 mm mesh) from each cylinder were then separately extracted with KCl and NO3- and NH4+ concentrations were measured. Nitrate and NH4+ concentrations of all samples were then converted to a content basis by multiplying their values with fine fraction bulk density. Net N mineralisation was thereafter calculated as the difference between the N content of the samples collected at the end of the three-month incubation (including the N extracted from the bottom resin bag) and the N content at the beginning of the incubation (Risch and others 2015).
Soil CO2 emissions were measured every two weeks between 0900 and 1700 hrs from early May through late September 2013 with a PP-Systems SRC-1 soil respiration chamber (15 cm high, 10 cm diameter; closed circuit) attached to a PP-Systems EGM-4 infrared gas analyser (PP-Systems, Amesbury, MA, USA) on two locations per plot (Risch and others 2013). The chamber was placed on randomly placed, permanently installed PVC collars (10 cm diameter) driven 5 cm into the soil at the beginning of the study (Risch and others 2013). Freshly germinated plants growing within the collars were removed prior to each measurement to avoid measuring plant respiration or photosynthesis. The two measurements collected per plot and sampling date were averaged.
Soil moisture (with time domain reflectometry; Field-Scout TDR-100, Spectrum Technologies, Plainfield, Illionois, USA) and temperature (with a waterproof digital pocket thermometer; Barnstead International, Dubuque, Iowa, USA) were measured at five random locations per plot every two weeks during the growing seasons during the experiment for the 0 to 10 cm depth (Risch and others 2013, 2015). As soil moisture and soil temperature were highly negatively correlated (Risch and others 2013), we only used soil moisture for this study. We used plot-level averages of all values available to capture soil moisture variability during the five years of the experiment. The results remained unchanged when we only used soil moisture from the 2013 growing season.
Numeral calculations and statistical analyses
Ecosystem coupling. We conducted principal component analyses (PCAs; unscaled) at the complete dataset level using the abundances of each taxonomical entity to describe each of the five different communities used in this study: aboveground-dwelling invertebrates, vascular plants, soil microorganisms, soil arthropods and soil nematodes. We retained the first two components (PCA axis 1 and PCA axis 2) of each analysis as we found them to adequately represent the temporal and spatial variability of our 90 treatment plots in previous studies55,67. Together they explained a total of 71.70% of the variation for aboveground invertebrates, 44.36% for plants, 44.85% for soil microorganisms, 61.85% for soil arthropods and 77.19% for soil nematodes. In addition, we used soil pH and soil organic C content as a proxy for soil chemical properties, soil bulk density as a proxy for soil physical properties and soil moisture (negatively correlated with soil temperature) as a proxy for soil micro-climatic conditions for an overall total of fourteen constituents.
We calculated ecosystem coupling9 for each exclosure treatment within each vegetation type (i.e., 2  5 treatment combinations in total) as an integrated measure of pairwise ecological interactions between ecosystem constituents representing ecological communities and the soil abiotic environment. These ecological interactions are defined by non-parametric Spearman rank correlation analyses between two constituents, excluding interactions involving two abiotic constituents (e.g., soil pH vs. soil moisture) and interactions between the first (PC1) and second (PC2) component of each community type, as these are orthogonal by definition. Interactions between abiotic constituents were excluded from the analyses because the focus of our study was on communities and how they interact with one another and their surrounding environment; therefore, including abiotic-abiotic interactions was not of interest here. Given that the effectiveness of our experimental design resulted in that no community composition data of aboveground-dwelling invertebrates was available for the “None” plots (all animals excluded), only thirteen instead of fourteen constituents were included in the ecosystem coupling calculations for this treatment. The complete absence of aboveground invertebrates represents the most extreme case of disturbance between aboveground animal communities and the rest of the ecosystem constituents. This may have resulted in a slight overestimation of ecosystem coupling for these plots.
	Average ecosystem coupling was calculated as follows:
Ecosystem coupling=
where Xi is the absolute Coupling was calculated value of the Spearman’s rho coefficient of the ith correlation for each treatment within each vegetation type (i.e., based on nine replicates each), considering and n is the number of pairwise comparisons considered (n = a total of 80; interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).	Coupling was calculated for each treatment within each vegetation type (i.e., based on nine replicates each), considering a total of 80 interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).
To establish whether constituents were significantly and positively coupled within treatments (i.e., the average of their correlation coefficients were greater than in a null model where correlation only happens by chance), we calculated one-tailed p-values based on permutation tests with 999 permutations.

We considered six ecosystem functions and process rates commonly used to assess ecosystem functioning (Meyer and others 2015; Manning and others 2018). Plant N content represents a measure of forage quality, while plant richness has been shown to stabilise biomass production, thus allowing the system to respond to changes in herbivory. Soil net N mineralisation, soil respiration, root biomass, and microbial biomass represent fluxes or stocks of energy. For all functions and processes higher values represent higher functioning (Manning and others 2018). All these variables were measured in the last year of the experiment (2013). We then quantified ecosystem multifunctionality using the multiple threshold approach (Byrnes and others 2014; Manning and others 2018), which considers the number of functions that are above a certain threshold, over a series of threshold values (typically 10-99%) that are defined based on the maximum value of each function. We weighted all our functions equally for these calculations (Manning and others 2018). The number of functions in a plot with values higher than a given threshold value for the respective function is summed up. The sum represents ecosystem multifunctionality for that plot. Given that choosing any particular threshold as a measure of ecosystem multifunctionality is arbitrary, we calculated the average of thresholds from 10-90% (in 10% intervals) as a more integrated representation of ecosystem multifunctionality.
We used Pearson correlations to explore the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and ecosystem multifunctionality by calculating the slopes of all relationships between ecosystem coupling and multifunctionality for all thresholds between 10 and 99%. We also related ecosystem coupling with the average of multifunctionality at thresholds between 30-80% as explained before and considered this correlation as a robust indication of the type of association between these two variables. In addition, we explored the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and individual ecosystem functions. The effects of exclosures and vegetation type on individual functions and multifunctionality were evaluated using linear mixed effects models ('lme' function of the nlme package), with exclosure and vegetation type as fixed effects and fence as a random factor. All statistical analyses and numerical calculations were done in R version 3.4.0 (R Core Team 2016).

#References

- Anderson J, Domsch K. 1978. A physiological method for the quantitative measurement of microbial biomass in soil. Soil Biol Biochem 10:215–21.
- Blackwood CB, Marsh T, Kim S-H, Paul EA. 2003. Terminal Restriction Fragment Length Polymorphism Data Analysis for Quantitative Comparison of Microbial Communities. Appl Environ Microbiol 69:926–32. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143601/
- Bongers T. 1988. De nematoden von Nederland. Schoorl, The Netherlands: Pirola
- Byrnes JEK, Gamfeldt L, Isbell F, Lefcheck JS, Griffin JN, Hector A, Cardinale BJ, Hooper DU, Dee LE, Duffy JE. 2014. Investigating the relationship between biodiversity and ecosystem multifunctionality: Challenges and solutions. Methods Ecol Evol 5:111–24.
- Crossley DAJ, Blair JM. 1991. A high-efficiency low-technology Tulgren-type extractor for soil microarthopods. Agric Ecosyst Environ 34:187–92.
- Dudley N. 2008. Guidelines for applying protected area managment categories. Gland: IUCN
- Frank DA, McNaughton SJ. 1990. Aboveground biomass estimation with the canopy intercept method: A plant growth form caveat. Oikos 57:57–60.
- Haynes AG, Schütz M, Buchmann N, Page-Dumroese DS, Busse MD, Risch AC. 2014. Linkages between grazing history and herbivore exclusion on decomposition rates in mineral soils of subalpine grasslands. Plant Soil 374.
- Hodel M, Schütz M, Vandegehuchte ML, Frey B, Albrecht M, Busse MD, Risch AC. 2014. Does the aboveground herbivore assemblage influence soil bacterial community composition and richness in subalpine grasslands? Microb Ecol 68:584–95.
- Liu WT, Marsh TL, Cheng H, Forney LJ. 1997. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ Microbiol 63:4516–22. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC168770/
- Manning P, van der Plas F, Soliveres S, Allan E, Maestre FT, Mace G, Whittingham MJ, Fischer M. 2018. Redefining ecosystem multifunctionality. Nat Ecol Evol 2:427–36. https://doi.org/10.1038/s41559-017-0461-7
- Meola M, Lazzaro A, Zeyer J. 2014. Diversity, resistance and resilience of the bacterial communities at two alpine glacier forefields after a reciprocal soil transplantation. Environ Microbiol 16:1918–34. https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.12435
- Meyer ST, Koch C, Weisser WW. 2015. Towards a standardized Rapid Ecosystem Function Assessment (REFA). Trends Ecol Evol 30:390–7. http://www.sciencedirect.com/science/article/pii/S0169534715000968
- Norman R., Stucki JW. 1981. The determination of nitrate and nitrite in soil extracts by ultraviolet spectrophotometry. Soil Sci Soc Am J 45:347–53.
- Ochoa-Hueso R. 2016. Non-linear disruption of ecological interactions in response to nitrogen deposition. Ecology 87:2802–2814.
- Oostenbrink M. 1960. Estimating nematode populations by some selected methods. In: Sasser NJ, Jenkins WR, editors. Nematology. Chapel Hill, NC, USA: University of North Carolina Press. pp 85–101.
- R Core Team. 2016. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing
- Risch AC, Haynes AG, Busse MD, Filli F, Schütz M. 2013. The response of soil CO2 fluxes to progressively excluding vertebrate and invertebrate herbivores depends on ecosystem type. Ecosystems 16:1192–202.
- Risch AC, Schütz M, Vandegehuchte ML, Van Der Putten WH, Duyts H, Raschein U, Gwiazdowicz DJ, Busse MD, Page-Dumroese DS, Zimmermann S. 2015. Aboveground vertebrate and invertebrate herbivore impact on net N mineralization in subalpine grasslands. Ecology 96:3312–22.
- Schütz M, Risch AC, Achermann G, Thiel-Egenter C, Page-Dumroese DS, Jurgensen MF, Edwards PJ. 2006. Phosphorus translocation by red deer on a subalpine grassland in the Central European Alps. Ecosystems 9:624–633.
- Schütz M, Risch AC, Leuzinger E, Krüsi BO, Achermann G. 2003. Impact of herbivory by red deer (Cervus elaphus L.) on patterns and processes in subalpine grasslands in the Swiss National Park. For Ecol Manage 181:177–88.
- Vandegehuchte ML, van der Putten WH, Duyts H, Schütz M, Risch AC. 2017a. Aboveground mammal and invertebrate exclusions cause consistent changes in soil food webs of two subalpine grassland types, but mechanisms are system-speciﬁc. Oikos 126:212–23.
- Vandegehuchte ML, Raschein U, Schütz M, Gwiazdowicz DJ, Risch AC. 2015. Indirect short- and long-term effects of aboveground invertebrate and vertebrate herbivores on soil microarthropod communities. PLoS One 10:e0118679.
- Vandegehuchte ML, Schütz M, de Schaetzen F, Risch AC. 2017b. Mammal-induced trophic cascades in invertebrate food webs are modulated by grazing intensity in subalpine grassland. J Anim Ecol 86:1434–46.
- Vandegehuchte ML, Trivellone V, Schütz M, Firn J, de Schaetzen F, Risch AC. 2018. Mammalian herbivores affect leafhoppers associated with specific plant functional types at different timescales. Funct Ecol 32:545–55.
- Wirthner S, Frey B, Busse MD, Schütz M, Risch AC. 2011. Effects of wild boar (Sus scrofa L.) rooting on the bacterial community structure in mixed-hardwood forest soils in Switzerland. Eur J Soil Biol 47:296–302. http://dx.doi.org/10.1016/j.ejsobi.2011.07.003
- Zumsteg A, Luster J, Göransson H, Smittenberg RH, Brunner I, Bernasconi SM, Zeyer J, Frey B. 2012. Bacterial, Archaeal and Fungal Succession in the Forefield of a Receding Glacier. Microb Ecol 63:552–64. https://doi.org/10.1007/s00248-011-9991-8

ecosystem-coupling

display_name: ecosystem-coupling

ecosystem-functions

display_name: ecosystem-functions

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

exclusion

display_name: exclusion

grassland

display_name: grassland

grazing

display_name: grazing

invertebrates

display_name: invertebrates

subalpine

display_name: subalpine

swiss-national-park

display_name: swiss-national-park

vertebrates

display_name: vertebrates

en: Ecosystem coupling and multifunctionality - exclosure experiment


en: population-evolution biological-control dryocosmus-kuriphilus pest-invasion castanea-sativa torymus-sinensis southern-switzerland

en:
Data has been collected from summer 2011 to 2016.

Main reasons for NA values:

-	D. kuriphilus still not arrived in the site.
-	No galls were found during the field sampling.
-	Site has not been sampled in the correspondent year because of time, weather or money constrains.
-    T. sinensis not found in the galls

Variable description:

Year = sampling year.

Coordinate, elevation, slope, aspect = average site characteristics.

ts_arrival = T. sinensis arrival year.

dk_arrival = D. kuriphilus arrival year.

dk_parasitism_by_ts = D. kuriphilus parasitism rate by T. sinensis. Formula: (no. of T. sinensis/ no. of D. kuriphilus chambers) * 100 at gall level.

dk_infestation = Percentage of attacked buds. Formula: no. of attacked buds / no. of total developed buds*100 (as presented in Kotobuki et al. 1985) at shoot level.

ts_per_bud = number of alive T. sinensis individual calculated at bud level.

dk_per_bud = number of alive D. kuriphilus individual calculated at bud level.

dk_pot_per_bud = overall number (dead and alive) D. kuriphilus individual calculated at bud level.

total_gall_nr_per_bud = average gall number per bud.

chamber_per_galls = average D. kuriphilus chamber number per gall.

For details see the submitted publication with the same name

References
Kotobuki, K., Mori, K. and Sato, Y. (1985) ‘2 methods to estimate the tree damage by chestnut gall wasp Dryocosmus-kuriphilus’, Bulletin of the fruit tree research station A (Yatabe), 2(12), pp. 29–36.

en:
This dataset contains the population evolution of a pest and its biocontrol agent in terms of presence proportion at gall level and absolute number of insects.

The study area extends from the Cuneo region (Piedmont, Italy) to southern Switzerland. In order to provide a complete range of data covering the entire process from the pest arrival to complete biological control by its natural enemy T. sinensis, a space-for-time substitution approach has been adopted so as to create a temporal gradient of the epidemic stages over the whole study area.

The southernmost Swiss sites roughly represent the arrival and establishment of the pest without the presence of the natural enemy, the central ones the early epidemic stage and the epidemic peak, whereas the northern ones the end of the epidemic with the beginning of the biocontrol. The Italian ones represent the beginning of the equilibrium between the two population as well as the situation with stable T. sinensis populations on the long term.

These data are used in the paper entitled: Torymus sinensis local and regional early population dynamics in the Insubrian and Piedmont regions

biological-control

display_name: biological-control

castanea-sativa

display_name: castanea-sativa

dryocosmus-kuriphilus

display_name: dryocosmus-kuriphilus

pest-invasion

display_name: pest-invasion

population-evolution

display_name: population-evolution

southern-switzerland

display_name: southern-switzerland

torymus-sinensis

display_name: torymus-sinensis


maintainer: Zhao

en: austin planning driving-factors land-dynamics urban-land-use

en: Potential driving factors of urban transformations of Austin over 25 years

media_type: application/zip

format: ZIP

en: In this study, the Austin metropolitan area, Texas, U.S., one of the fastest urban transformations and transformations regions, is selected to test the hypothesis that spatial planning and policies are important factors of urban transformations. Despite ample previous work in understanding the interactions between human and urban form transformation at specific areas, the actual interventions and outcomes of planning and policies (e.g., ‘smart growth’) on urban forms have been poorly measured. In this study, the potential influencing factors of urban transformations of Austin over 25 years were selected and collected.

austin

display_name: austin

driving-factors

display_name: driving-factors

land-dynamics

display_name: land-dynamics

planning

display_name: planning

urban-land-use

display_name: urban-land-use


en: swiss-forest-lab long-term meteo par climate-change temperature relative-humidity meteorology precipitation wind-speed

en: Data plot of the forest station (LEB)

media_type: application/pdf

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

format: PDF

mimetype: application/pdf

en: Site photo of the forest station (LEB)

en: Site photo of the forest station from below (viewing upwards the slope)

mimetype: image/jpeg

en: Forest station data (LEB)

en: Data file.

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for one meteorological station in Lens in Switzerland which is located within a natural coniferous forest with Scots pine (_Pinus sylvestris_; 150-170 yrs)) as dominant tree species. The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Lens is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

en: Lens, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


maintainer: Matthias Haeni

en: temperature meteo long-term climate-change par precipitation wind-speed swiss-forest-lab meteorology relative-humidity

en: Data plot of the forest station (ALB)

en: Site aerial photo (Alpthal)

en: Site aerial photo from the Alpthal forest station. Source: Google. 2016. Site aerial photo from the Alpthal forest station. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Forest station data (ALB)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for one meteorological station in Alpthal in Switzerland which is located within a natural coniferous forest (ALB) with Norway spruce (_Picea abies_; 180-230 yrs) as dominant tree species. The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Alpthal is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Alpthal, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


maintainer: Albrecht von Boetticher

en: hangmuren interfoam barrier-impact-simulation finite-volumes debris-flow dberisintermixing shallow-landslide natural-hazard murgang

en: debrisInterMixing for OF-Version 6

mimetype: application/zip

en: Installation Instructions for debrisInterMixing-6

en: These are the installation instructions for debrisInterMixing4

en:
This version of debrisInterMixing is based on OpenFOAM Version 4.x and was tested on Ubuntu 18.04.1 LTS.
Please follow the instructions given in ReadMe_debrisInterMixing4.pdf for installation.

en:
Here the updated versions of debrisInterMixing are provided for download. The first OpenFoam-compatible Version 2.3.x are available as supplement to v. Boetticher, A., Turowski, J. M., McArdell,W. B., Rickenmann, D., Hürlimann, M., Scheidl, C., and Kirchner, J. W.: DebrisInterMixing-2.3: A Finite Volume solver for three dimensional debris flow simulations based on two calibration parameters. Part two: model validation with experiments. Geoscientific Model Development, 10, 11: 3963-3978. doi: 10.5194/gmd-10-3963-2017.
DebrisInterMixing is a Volume-of-Fluid based Finite Volume code that accounts for shear-thinning sensitive shares of fine sediment suspension together with pressure-sensitive components of the gravel grains within debris flow mixtures. All model properties can be derived from a material sample except for a grid-sensitive calibration parameter.
For more information, please contact albrecht.vonboetticher@wasserbau.ch.

barrier-impact-simulation

display_name: barrier-impact-simulation

dberisintermixing

display_name: dberisintermixing

debris-flow

display_name: debris-flow

finite-volumes

display_name: finite-volumes

hangmuren

display_name: hangmuren

interfoam

display_name: interfoam

murgang

display_name: murgang

natural-hazard

display_name: natural-hazard

shallow-landslide

display_name: shallow-landslide

en: Three-dimensional debris flow simulation tool debrisInterMixing


en: climate-change precipitation temperature long-term relative-humidity meteo par wind-speed meteorology swiss-forest-lab

en: Site aerial photo (Vordemwald)

en: Site aerial photo from both Vordemwald stations. Source: Google. 2016. Site aerial photo from both Vordemwald stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Data plot of the field station (VOF)

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

en: Forest station data (VOB)

en: Data file.

en: Data plot of the forest station (VOB)

en: Site photo of the forest station (VOB)

en: Site photo of the forest station from the side.

en: Field station data (VOF)

en: Site photo of the field station (VOF)

en: Site photo of the field stationfrom the side.

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Vordemwald in Switzerland where one station is located within a natural mixed forest stand (VOB) with European silver fir (_Abies alba_; 110 yrs) and oak trees (_Quercus sp._; 190-210 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, VOF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Vordemwald is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

en: Vordemwald, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1996 onwards


maintainer: Frank Techel

en: shapefile avalanches observations snow alps

en: Selected wet snow avalanche activity data Davos, Switzerland (2011-2014)

en: Zip archive with shapefiles for the selected wet snow avalanche polygons, including metadata.

en:
Polygons of wet snow avalanches in the Davos area, as documented by the Swiss avalanche warning service. The georeferenced outlines of the avalanches contain both the release as well as the deposit area, but without separating between both.

The dataset is a subset of the total record of 1615 avalanches classified as wet snow avalanches from October 2011 - September 2014, containing those 255 avalanches exceeding 0.0125 km^2. Every polygon comes with meta data, including the date of occurrence.

This dataset is the underlying dataset to:
Wever, N., Vera Valero, C. and Techel, F. (2018) _Coupled snow cover and avalanche dynamics simulations to evaluate wet snow avalanche activity_. Submitted to J. Geophys. Res., in review.

alps

display_name: alps

avalanches

display_name: avalanches

observations

display_name: observations

shapefile

display_name: shapefile

snow

display_name: snow

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.


en: strategic-projects land-use-change urban-transformations strategic-spatial-planning spatial-planning europe large-scale-urban-development-projects land-use-transformations

en: QCA data set large-scale urban development projects

media_type: text/plain

en: Please refer to the sections: 3. The data set lsUDPs: 3.1 Selection of cases and data collection; 3.2 Data management and operationalization in the Metadata for further details on this resource.

format: TXT

mimetype: text/plain

en: QCA R script large-scale urban development projects Europe

en:
For the set up of this R script, we used the “QCA” (Duşa, 2019) and “SetMethods” (Oana and Schneider, 2018) R packages.

The following further resources were also used:
Dusa, A. (2019). QCA with R: A comprehensive resource. Springer: Berlin.
Schneider, C. Q., & Wagemann, C. (2012). Set-theoretic methods for the social sciences. A guide to qualitative comparative analysis. Cambridge: Cambridge University Press.
Thomann, E., Oana, I.-E., & Wittwer, S. (2018). Performing fuzzy- and crisp set QCA with R. A user-oriented beginner's guide. Retrieved from http://www.evathomann.com/links/qca-r-manual

en:
Table of Content: 1. General context of the data set "lsUDPs" ; 2. Background and aims of the study using the data set lsUDPs; 3. The data set lsUDPs: 3.1 Selection of cases and data collection; 3.2 Data management and operationalisation

1. General context of the data set "lsUDPs"
The data set "lsUDPs" has been generated as part of the CONCUR research project (https://www.wsl.ch/en/projects/concur.html) led by Dr. Anna M. Hersperger and funded by the Swiss National Science Foundation (ERC TBS Consolidator Grant (ID: BSCGIO 157789) for the period 2016-2020. The CONCUR research project is interdisciplinary and aims to develop a scientific basis for adequately integrating spatial policies (in this case, strategic spatial plans) into quantitative land-change modelling approaches at the urban regional level. The first stage (2016-2017) of the CONCUR project focussed on 21 urban regions in Western Europe. The urban regions were selected through a multi-stage strategy for empirical research (see Hersperger, A. M., Grădinaru, S., Oliveira, E., Pagliarin, S., & Palka, G. (2019). Understanding strategic spatial planning to effectively guide development of urban regions. Cities, 94, 96–105. https://doi.org/10.1016/j.cities.2019.05.032 ).

2. Background and aims of the study using the data set lsUDPs
As part of the CONCUR project, a specific task was to examine the relationship between strategic spatial plans and the formulation and implementation (i.e. urban land change) of large-scale urban development projects in Western Europe.
Strategic urban projects are typically large-scale, prominent urban transformations implemented locally with the aim to stimulate urban growth, for instance in the form of urban renewals of deprived neighborhoods, waterfront renewals and transport infrastructures. While strategic urban projects are referred to in the literature with multiple terms, in the CONCOR project we call them large-scale urban development projects (lsUDPs).
Previous studies acknowledged both local and supra-local (or structural) factors impacting the context-specific implementation of lsUDPs. Local governance factors, such as institutional capacity, coordination among public and private actors and political leadership, intertwine with supra-local conditions, such as state re-scaling processes and devolution of state competencies in spatial planning, de-industrialisation and increasing social inequality. Hence, in implementing lsUDPs, multi-scalar factors act in combination.
Because the formulation and implementation of lsUDPs require multi-scalar coordination among coalitions of public and private actors over an extended period of time, they are generally linked to strategic spatial plans (SSPs). Strategic spatial plans convey collective visions and horizons of action negotiated among public and private actors at the local and/or regional level to steer future urban development, and can contain legally binding dispositions, but also indicative guidelines.
The key question remains as to what extent large-scale urban development projects and strategic spatial plans can be regarded as aligned. By alignment, or “concordance”, we mean that strategic projects are formulated and implemented as part of the strategic planning process (“high concordance”), or that the strategic role of projects is reconfirmed in (subsequent) strategic plans (“moderate concordance”). Lack of concordance is found when lsUDPs have been limitedly (or not at all) acknowledged in strategic spatial plans.
We assume that certain local and supra-local factors, characterising the development of the projects, foster (but not strictly “cause”) the degree of alignment between lsUPDs and SSPs. In this study, we empirically examine how, and to what extent, the concordance between 38 European large-scale urban development projects and strategic plans (outcome: CONCOR) has been enabled by five multi-scalar factors (or conditions): (i) the role of the national state (STATE), (ii) the role of (inter)national private actors (PRIVATE), (iii) the occurrence of supra-regional external events (EVENTS), (iv) the degree of transport connectivity (TRANSP), and (v) local resistance from civil society (RESIST). We adopted a (multi-data) case-based qualitative strategy for empirical research and applied the formalised procedure of within- and cross-case comparison offered by fuzzy-set Qualitative Comparative Analysis appropriate for the goal of this study. Based on set theory, QCA formally integrates contextual sensitivity to case specificities (within-case knowledge) with systematic comparative analysis (across-case knowledge).
The research question the data set has been created to reply to is the following: which conditions, and combinations of conditions, enable the concordance between large-scale urban development projects and strategic spatial plans?
The conditions (“independent variables”) considered are. STATE: the set of large-scale urban projects characterized by a high degree of state intervention and support in their formulation and implementation, PRIVATE: the set of large-scale urban projects characterized by a high degree of involvement of (inter)national private actors in their formulation and implementation, EVENTS: the set of large-scale strategic projects whose formulation and implementation have been strongly affected by unforeseen international events and/or global trends, TRANSP: the set of large-scale strategic projects with a high degree of road and/or transit connectivity, and RESIST: set of large-scale strategic projects whose realization has been characterized by resistances that have substantially delayed or modified the project implementation.
The outcome (“dependent variable”) under analysis is CONCOR: the set of large-scale urban projects having a high degree of concordance/alignment/integration with strategic spatial plans

3. The data set lsUDPs

   3.1 Selection of cases and data collection
To generate the current data set on large-scale urban development projects in European urban regions (data set "lsUDPs"), we identified 35 large-scale urban development projects in a sample of the 21 Western urban regions considered in the CONCUR project (see supra, Hersperger et al. 2019): Amsterdam, Barcelona, Copenhagen, Hamburg, Lyon, Manchester, Milan, Stockholm, Stuttgart. The criteria we followed to identify the 35 large-scale urban development projects are: geographical location, size (large-scale), site (located either in the city core or in the larger urban region) and urban function (e.g. housing, transportation infrastructures, service and knowledge economic functions). Employing these criteria facilitated the selection of diverse large-scale urban development projects while still ensuring sufficient comparability.
In 2016, we performed 47 in-depth interviews with experts in urban and regional planning and large-scale strategic projects and infrastructure (i.e. academics and practitioners) about the formulation, implementation and development (1990s–2010s) of each project in each of the 9 selected urban regions. On average, each interviewee answered questions on 3.1 large-scale urban development projects. Three cases were subdivided into two cases because a clear differentiation between specific implementation stages was identified by the interviewees (expansion of the Barcelona airport, cases “bcn_airport80-90” and “bcn_airport00-16”; realisation of Lyon Part-Dieu, cases “lyo_partdieu70-90” and “lyo_partdieu00-16”; MediaCityUK, cases “man_salfordquays80-00” and “man_mediacityuk00-16”). Therefore, from the initial 35 cases, the final number of analysed cases in the lsUDPs dataset is 38.

    3.2 The data set lsUDPs: Data management and operationalisation
Interviews were fully transcribed and analysed through MAXQDA (version 12.3, VERBI GmbH, Berlin, Germany), and intercoder agreement was evaluated on a sample of nine interviews. We also compiled “synthetic case descriptions” (SCD) for each case (totalling more than 160 SCDs) to spot potential inconsistencies among interviewees’ accounts and to facilitate completion of the “calibration table” for each case (see below). An online expert survey distributed to the interviewees (response rate 78%) helped systematise the information collected during the interviews. We also consulted both academic and gray literature on the case studies to check for possible ambiguity and inconsistencies in the interview data, and to solve discrepancies between our assigned set membership scores and questionnaire values. Site visits were also carried out to retrieve additional information on the selected cases.
For each case (i.e. each of the 38 selected large-scale urban development projects), we operationalised each condition (i.e. STATE, PRIVATE, EVENTS, TRANSP, RESIST) and the outcome (CONCOR) in terms of sets, for subsequent application of Qualitative Comparative Analysis. This process is called “calibration”; we used a number of indicators for each condition to qualitatively assess each large-scale project across the conditions. The case-based qualitative assessment was then transformed into fuzzy-set membership values. Fuzzy-set membership values range from 0 to 1, and should be conceived as “fundamentally interpretative tools” that “operationalize theoretical concepts in a way that enhances the dialogue between ideas and evidence” (Ragin 2000:162, in “Fuzzy-set Social Science”. Chicago: University Press). We employed a four-value fuzzy-set scale (0, 0.33, 0.67, 1) to “quantify” into set membership scores the individual histories of cases retrieved from interview data. Only the condition TRANSP was calibrated as a crisp-set (0, 1).
The translation of qualitative case-based information into numerical fuzzy-set membership values was iteratively performed by populating a calibration table following standard practices recently emerged in QCA when dealing with qualitative (interview) data.

europe

display_name: europe

land-use-change

display_name: land-use-change

land-use-transformations

display_name: land-use-transformations

large-scale-urban-development-projects

display_name: large-scale-urban-development-projects

spatial-planning

display_name: spatial-planning

strategic-projects

display_name: strategic-projects

strategic-spatial-planning

display_name: strategic-spatial-planning

urban-transformations

display_name: urban-transformations

en: lsUDPS Large-scale urban development projects in European urban regions


en: afforestation mountain-forest treeline long-term-experiment stillberg

en: Documentation

en: This data documentation is outdated and has been replaced with an updated version (see description).

mimetype: application/msword

en: Site photo

en: This site photo is outdated and has been replaced with an updated version (see description).

en: Dataset V1

en: This dataset is outdated and has been replaced with an updated version (see description).

format: GDB

en:
 Important
This EnviDat entry is outdated. The most recent, usable version of the data can be found under the new EnviDat entry "Long-term afforestation experiment at the Alpine treeline site Stillberg, Switzerland." The entry can be found under this link <https://www.envidat.ch/dataset/afforestation-stillberg> and with this DOI 10.16904/envidat.397.

afforestation

display_name: afforestation

long-term-experiment

display_name: long-term-experiment

mountain-forest

display_name: mountain-forest

stillberg

display_name: stillberg

treeline

display_name: treeline

en: Long-term treeline research dataset at Stillberg, Davos


maintainer: Elisabeth Hafner

en: reliability avalanche-mapping avalanche-size-estimation remote-sensing avalanche

en: AvalancheReliabilityData

en: The .zip contains all data used to conduct the reliability study as well as the results, a detailed description of the included files is contained in the included pdf. All geospatial data is provided in the Swiss Coordinate System: CH1903+ / LV95 (EPSG 2056). For any use of the data the proper citation provided is obligatory!

mimetype: application/x-zip-compressed

en:
This dataset contains the data used and collected to investigate the reliability of avalanche size estimates and avalanche outlines (see related publications). Namely this is

 Avalanche size estimates

* the 10 pictures depicting avalanches
* the survey results from the 170 participants

 Avalanche outlines mapped from oblique photographs
* the pictures of the six avalanches
* the avalanche outlines mapped by the 10 participants
* the reference mapped from the georeferenced photographs

 Avalanche outlines from remotely-sensed imagery
* the orthophotos in the four different resolutions (2m, 1m, 50cm, 25cm)
* the area of interest (AoI) delimiting the research area
* the avalanche mappings in each resolution for all five participants
* the shade masks used to separate illuminated and shaded areas

The acquisition of the orthophotos has been partially supported by the Swiss National Science Foundation (SNF; Grant N°200021_172800).

avalanche

display_name: avalanche

avalanche-mapping

display_name: avalanche-mapping

avalanche-size-estimation

display_name: avalanche-size-estimation

reliability

display_name: reliability

remote-sensing

display_name: remote-sensing

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Data reliability study: avalanche size estimates and outlines


en: detritus yellowstone biogeochemical-cycling carcass carrion decomposition wolf bacteria fungi soil-biodiversity

en:
Site	ID for different carcasses across the Yellowstone Landscape
Location	Sampling location, either under carcass or control plot
Species	Indicating whether carcass was from bison or elk
Gender	Gener of carcass; F = female, M = male, ? = unknown
Lat	Latitude
Long	Longitude
DSK	Days since kill; in days
Soil.N	Soil N concentration (%)
Soil.C	Soil C concentration (%)
Veg.C	Vegetation C concentration (%); dominant grasses sampled
Veg.N	Vegetation N concentration (%); dominant grasses sampled
Veg.CtoN	Vegetation C to N ratio; dominant grasses sampled
Soil.resp	Soil respiration (g m-2 h-1)
soil.temp	Soil temperature (°C)
Soil.moist	Soil moisture (volumentric; %)
CRR	Cotton rotting rate
bacteria.richness	Bacteria richness
bacteria.diversity	Bacteria diverstiy
fungi.richness	Fungi richness
fungi.diversity	Fungi diversity
bacteria.abundance	Bacteria abundance
fungi.abundance	Fungi abundance

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:

Risch, A.C., Frossard, A., Schütz, M., Frey, B., Morris, A.W., Bump, J.K. (accepted) Effects of elk and bison carcasses on soil microbial communities and ecosystem functions in Yellowstone, USA. (accepted). Functional Ecology
doi: ...

Methods
Study area and study sites
This study was conducted in YNP’s Northern Range (NR), located in north-western Wyoming and south-western Montana, USA (~44.9163° N, 110.4169° W). The NR expands over ~1000 km2 and features long cold winters and short dry summers. Grasslands and shrublands dominate the NR that is the home of large migratory herds of bison (winter counts 2017: ~3919 individuals; Geremia, Wallen, & White, 2017) and elk (~5349 individuals) as well as their main predators, approximately five packs of wolves with a total of 33 individuals (Smith et al., 2017). As part of a long-term research program within YNP, wolf predation has been studied since their reintroduction in 1995.
For our study, we received ground-truthed coordinates of bison and elk carcasses from winter 2016/17 (November 2016 through April 2017) from the YNP Wolf Project. Between June 20 and July 1, 2017, we visited 24 carcasses in total. At five sites, we could not sample as the carcasses were no longer found. In total we located remains (hairmats, rumen content, bones, teeth) of 19 adult male and female carcasses (7 bison, 12 elk; Supplementary Table 1). Live body weights of adult bison and elk are approximately 730 kg (male bison), 450 kg (female bison), 330 kg (male elk), and 235 kg (female elk, Meagher, 1973; Quimby & Johnson, 1951).
The kills and subsequent consumption happened between 34 and 173 days prior to our sampling (hereafter “days since kill”, DSK), for which we accounted in our statistics. Note that wolves and other scavengers consumed the soft tissue of the carcasses quickly, hence, there is close to no soft tissue left for decomposition as compared to an intact body left on the soil surface. The 19 carcass sites covered the extent of YNP’s NR, with both bison and elk carcasses showing similar distributions; elevation ranged from 1703 to 2884 m a.s.l. (Supplementary Fig 1 & Supplementary Table 1). The carcasses were all located in grassland or sage-brush shrubland, with or without sparsely scattered trees, and both bison and elk carcasses showed the same distribution of DSK. At each study site, we selected a reference plot (hereafter “control”) that was of comparable size, slope aspect and vegetation to the carcass location (hereafter “carcass”). The control was at least 10 m away (Danell, Berteaux, & Brathen, 2002; Melis et al., 2007) from the carcass itself to ensure the absence of potential direct and indirect carcass effects (paired design; (Bump, Webster, et al., 2009; Bump, Peterson, et al., 2009).

Ecosystem functions and soil properties
We randomly collected 50 g of mineral soil from three locations on both control and carcass plots to a depth of 5 cm with sterile techniques and gently mixed the material to obtain a composite sample. Half the soil sample was immediately bagged in plastic bags (whirl packs), stored in a cooler with ice packs (~5 ºC), sieved (2-mm) and frozen within 4-6 hours of collection to assess soil microbial communities. For this purpose, we extracted total genomic DNA from 0.5 g soil using the PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany). DNA concentrations were measured using PicoGreen (Molecular Probes, Eugene, OR, USA). PCR amplifications of partial bacterial small-subunit ribosomal RNA genes (region V3–V4 of 16S rRNA) and fungal ribosomal internal transcribed spacers (region ITS2) were performed as described previously (Frey et al., 2016). Each sample consisting of 40 ng DNA was amplified in triplicate and pooled before purification with Agencourt AMPure XP beads (Beckman Colter, Berea, CA, USA) and quantified with the Qubit 2.0 fluorometric system (Life Technologies, Paisley, UK). Amplicons were sent to the Genome Quebec Innovation Center (Montreal, Canada) for barcoding using the Fluidigm Access Array technology and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA).
Quality control of bacterial and fungal reads was performed using a customized pipeline (Supplementary Table 2; Frey et al., 2016). Paired-ends reads were matched with USEARCH (Edgar & Flyvbjerg, 2015), substitution errors were corrected using Bayeshammer (Nikolenko, Korobeynikov, & Alekseyev, 2013) and PCR primers were trimmed (allowing for 1 mismatch, read length >300 bp for 16S and >200 bp for ITS primers) using Cutadapt (M. Martin, 2011). Sequences were dereplicated and singleton reads removed prior to clustering into operational taxonomic units (OTUs) at 97% identity using USEARCH (Edgar, 2013). The remaining centroid sequences were tested for the presence of ribosomal signatures using Metaxa2 (Bengtsson-Palme et al., 2015) or ITSx (Bengtsson-Palme et al., 2013). Taxonomic assignments of the OTUs were obtained using Bayesian classifier (Wang, Garrity, Tiedje, & Cole, 2007) with a minimum bootstrap support of 60% implemented in mothur (Schloss et al., 2009) by querying the bacterial and fungal reads against the SILVA Release 128 (Quast et al., 2013) and UNITE 8.0 (Abarenkov et al., 2010) reference databases for 16S and ITS OTUs, respectively.
Abundances of the bacterial 16S rRNA gene and fungal ITS amplicon were determined by quantitative real-time PCR (qPCR) on an ABI7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) as described previously (Frossard et al., 2018). The same primers (without barcodes) and cycling conditions as for the sequencing approach were used for the 16S and ITS qPCR. Three standard curves per target region were obtained using tenfold serial dilutions of plasmids generated from cloned targets (Frey, Niklaus, Kremer, Lüscher, & Zimmermann, 2011). Data were converted to represent mean copy number of targets per gram of soil (dry weight).
The other half of the soil sample was bagged in paper, dried to constant weight at 60°C, passed through a 2 mm sieve and analyzed for total C and N concentration with a CE Instruments NC 2100 soil analyzer (CE Elantech Inc., Lakewood NJ, USA). We also collected 20 mature and undamaged leaves of the dominant grass species growing on control and carcass sites, but taxa were not recorded. The plant material was dried at 60°C, finely ground till homogenized and also analyzed to obtain total C and N concentrations. Soil temperature (10 cm depth) was measured with a waterproof digital thermometer (Barnstead International, Dubuque IA, USA) at three locations each at the control and carcass site. Soil moisture (0 – 10 cm depth) was measured with time domain reflectometry (Field-Scout TDR-100; Spectrum Technologies, Plainfield IL, USA) at five randomly chosen points on control and carcass sites. We measured soil respiration at five randomly chosen points at both control and carcass sites with a PP-Systems SRC-1 soil respiration chamber (closed circuit) attached to a PP-Systems EGM-4 infrared gas analyzer (PP-Systems, Amesbury, MA, USA). For each measurement the soil chamber (15 cm high; 10 cm diameter) was tightly placed on the soil surface, after clipping plants to avoid measuring plant respiration or photosynthesis. Measurements were conducted over 120 s.
In addition, we assessed the decomposition rates of standardized OM using the cotton strip assay (Latter & Howson, 1977; Latter & Walton, 1988). Cotton cloth tensile strength loss (CTSL) is a measure of decomposition, and an index to express the combined effect of soil microclimatic, physical, chemical and biological properties on decomposition while accounting for OM quality (Latter & Walton, 1988; Risch, Jurgensen, & Frank, 2007; Withington & Sanford Jr., 2007). We placed five 20 cm wide x 13 cm long sheets of 100% unbleached cotton cloth (American Type SM 1/18’’, Warp: 34/1, Weft: 20/1, Weave plain, 29.5 picks/cm warp, 22 picks/cm weft, 237 g/m2; Daniel Jenny & Co., Switzerland;) at each carcass and control site vertically into the soil by making slits with a flat spade to a depth of 12 cm. We inserted each cloth with the spade, and then pushed the slit closed to assure tight contact with the soil. The cloths were retrieved after 18 to 27 days. After retrieval, the cloths were air-dried, remaining soil gently removed by hand, and 1.5 cm wide strips were cut at the 3.5-5.0 cm (top) and the 9-10.5 cm (bottom) soil depth. The strips were equilibrated at 50 % relative humidity and 20°C for 48 hours (climate chamber) prior to strength testing (Scanpro Awetron TH-1 tensile strength tester; AB Lorentzen and Wettre, Kista, Sweden). Cotton rotting rate (CRR) = (CTScontrol - CTSfinal/CTSfinal)1/3 * (365/t), where CTScontrol is the cotton tensile strength of a control cloth and CTSfinal the cotton tensile strength of the incubated sample, t is the incubation period in days. Control cloths were inserted into the ground and immediately retrieved to account for tensile strength loss associated with cloth insertion. We averaged the CRR of top and bottom strips for further analyses as no difference was found between the two. All sampling and cloth insertion took place between June 20 and July 1, 2017, cloths were retrieved between July 17 and 20, 2017. Soil respiration, average CRR, vegetation N concentration and vegetation C:N ratio are defined as ecosystem functions, soil C and N concentration, soil temperature and moisture as soil abiotic properties, and bacterial and fungal richness (number of taxa), diversity (Shannon) and abundance as soil biotic properties.

Statistical analyses
Univariate analyses for ecosystem functions, soil biotic and abiotic properties
We tested whether individual ecosystem functions, soil biotic and abiotic properties differed between carcass and control (“Location”), bison and elk (“Species”) and days since kill (“DSK”). For this purpose, we used linear mixed effect models (LMM, “nlme” package v 3.1 – 131.1 in R v 3.4.4; Pinheiro, Bates, DebRoy, & Sarkar, 2018; R Core Team, 2019) with Location, Species, Location x Species and DSK as fixed effects. Site was included as random effect to account for the paired design. We developed a separate model for all dependent variables. All but bacterial richness, fungal richness, fungal diversity and vegetation N concentration were natural-log transformed to meet model assumptions. For each LMM, we calculated contrasts to assess the specific comparisons we were interested in with the “lsmeans” package v 2.27-62 (Lenth & Love, 2018): 1) carcass vs control, 2) carcass bison vs control bison, and 3) carcass elk vs control elk. We also tested whether we had differences between bison and elk carcasses or the sites where bison and elk were killed and included contrasts 4) carcass bison vs carcass elk and 5) control bison vs control elk.
We calculated the log response ratio (LRR = ln[carcass/control]) to obtain carcass effects for all variables for both species separately. LRR < 0 indicates higher value at control compared to carcass, LRR > 0 indicates higher values at carcass compared control. We used LRRs for visualization and to assess spatial patterns in carcass effects across YNP. For this purpose we calculated the Moran’s I statistic for each ecosystem function, soil biotic and abiotic property based on a latitude-longitude matrix with the “moran.test” function in the “spdep” package version 1.1-3 (Bivand et al., 2019).
Multivariate analyses
Rare OTUs, defined as OTUs with a low abundance of reads, were retained in multivariate methods because they only marginally influence these analyses (Gobet, Quince, & Ramette, 2010). Bray–Curtis dissimilarity matrices were generated based on square-root-transformed matrices. We used Principal Coordinate Analyses (PCoA) to assess how soil bacterial and fungal communities differed between control and carcass of bison and elk (“vegan” package v 2.5-4, Oksanen et al., 2019). We then extracted PCoA axes scores 1 and 2 and used LMM (“nlme” package) with Location, Species, Location x Species and DSK as fixed effects. Site was, again, included as random effect. We again calculated the contrasts as described above using the “lsmeans” package. We also assessed how ecosystem functions, and soil abiotic and biotic properties were related to the soil bacteria and fungi community structure associated with bison and elk control and carcasses using the “envfit” function in the “vegan” package (Oksanen et al., 2019).
Indicator species analyses were performed using the multipatt function implemented in the “indicspecies” package version 1.7.6 with 100000 permutations (De Caceres & Jansen, 2016). This step allowed to identify OTUs that led to changes in multivariate patterns between control and carcass of both bison and elk separately (De Cáceres, Legendre, & Moretti, 2010). The multipatt function uses a point biserial correlation coefficient statistical test. Indicator OTUs were defined as bacterial and fungal OTUs with more than 50 sequences, i.e., removing rare taxa and taxa with low abundances containing little indicator information (Rime et al., 2015) and that were significantly correlated with Location (p < 0.05, correlation coefficient > 0.3). A heatmap of these OTUs were generated with the vegan and ggplot2 packages. The indicator analyses were performed in R version 3.3.3 (R Core Team, 2017).

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Please cite this paper together with the citation for the datafile.

bacteria

display_name: bacteria

biogeochemical-cycling

display_name: biogeochemical-cycling

carcass

display_name: carcass

carrion

display_name: carrion

decomposition

display_name: decomposition

detritus

display_name: detritus

fungi

display_name: fungi

soil-biodiversity

display_name: soil-biodiversity

wolf

display_name: wolf

yellowstone

display_name: yellowstone

en: Elk and bison carcasses in Yellowstone, USA