maintainer: M. Carol Resch

en: diversity vegetation long-term-experiment nematodes grassland topsoil-removal restoration

en: Plant community composition (plant species)

en:
Plot: Plot ID combining treatment code and plot number (in total 55 plots)

Treatment: Three restoration treatments and two reference treatments. Initial=intensively managed grasslands; Harvest only= multiple hay harvest (2-3 times) per years; Topsoil= Topsoil removal (upper 10-30 cm); Topsoil+Propagules= Topsoil removal (upper 10-30 cm) combined with introduction of target plant species; Target= species-rich ancient grasslands

column 3-252: Plant abundance per species (in total 252 plant species) expressed as cover percentage estimation according to van der Maarel (vdM 1979; 7 classes: 1-7); nomenclature according to Lauber & Wagner (1996)

en: Coordinates of one research plot were wrong. This was corrected in the current version of the file.

en: Nematode families

en:
Plot: Plot ID combining treatment code and plot number (in total 55 plots)

Treatment: Three restoration treatments and two reference treatments. Initial=intensively managed grasslands; Harvest only= multiple hay harvest (2-3 times) per years; Topsoil= Topsoil removal (upper 10-30 cm); Topsoil+Propagules= Topsoil removal (upper 10-30 cm) combined with introduction of target plant species; Target= species-rich ancient grasslands

column 3-41: Nematode abundance (individuals per 100g dry soil) per family; nomenclature according to Bongers 1988

en: Nemadotes and vegetation

en:
Plot: Plot ID combining treatment code and plot number (in total 55 plots)

Treatment: Three restoration treatments and two reference treatments. Initial=intensively managed grasslands; Harvest only= multiple hay harvest (2-3 times) per years; Topsoil= Topsoil removal (upper 10-30 cm); Topsoil+Propagules= Topsoil removal (upper 10-30 cm) combined with introduction of target plant species; Target= species-rich ancient grasslands

X: X-Coordinate

Y: Y-Coordinate

Ri.veg: Total number of plant species

Div.veg: Shannon diversity of plant species

Ri.nem: Total number of nematode families

Div.nem: Shannon diversity of nematode families

abund: Total nematode abundance (individuals per 100g dry soil)

MI: Maturity index according to Bongers 1990; calculations done in online tool NINJA (Sieriebriennikov, Ferris, & de Goede, 2014)

PPI: Plant parasite index according to Bongers 1990; calculations done in online tool NINJA (Sieriebriennikov, Ferris, & de Goede, 2014)

PPI.MI: Plant parasite to Maturity index according to Bongers, van der Meulen, & Korthals 1997; calculations done in online tool NINJA (Sieriebriennikov, Ferris, & de Goede, 2014)

CI: Channel index according to Ferris, Bongers, & de Goede 2001 (%); calculations done in online tool NINJA (Sieriebriennikov, Ferris, & de Goede, 2014)

EI: Enrichment index according to Ferris, Bongers, & de Goede 2001 (%); calculations done in online tool NINJA (Sieriebriennikov, Ferris, & de Goede, 2014)

SI: Structure index according to Ferris, Bongers, & de Goede 2001 (%); calculations done in online tool NINJA (Sieriebriennikov, Ferris, & de Goede, 2014)

He.a: Herbivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

He.ecto.a: Herbivorous ectoparasite nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

He.endo.a: Herbivorous endoparasite nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Ba.a: Bacterivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Fu.a: Fungivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Om.a: Omnivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Ca.a: Carnivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Om.Ca.a	Sum of omnivorous and carnivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014
CP1.a: C-P (colonizer-persister) class 1 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990; equivalent to enrichment indicators according to Ferris, Bongers, & de Goede 2001

CP2.a: C-P (colonizer-persister) class 2 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990; equivalent to stress indicators according to Ferris, Bongers, & de Goede 2001

CP3.a: C-P (colonizer-persister) class 3 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990

CP4.a: C-P (colonizer-persister) class 4 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990

CP5.a: C-P (colonizer-persister) class 5 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990

CP3-5.a: Sum of C-P3, C-P4 and C-P5 nematode abundance (individuals per 100g dry soil) equivalent to structure indicators according to Ferris, Bongers, & de Goede 2001

protect.sp.pro: plant species categorized into species of concern based on their red list status in Switzerland as well as their protection status in Switzerland and the Canton Zurich (Moser, Gygax, Bäumler, Wyler, & Palese, 2002)

tar.sp: Number of target species (all species recorded in the eleven target plots plus propagules of species applied by hand, resulting in a total of 143 species)

Indi.moist: Plant indicator values for soil moisture according to Landolt et al. 2010

Indi.nutri: Plant indicator values for soil nutrients according to Landolt et al. 2010

Management: level of mowing and manuring frequency (35-40 kg N ha-1 application-1); intensity1 = mowing once and no manure; intensity2 = 2-3 times mowing and no manure; intensity3 = 2-3 times mowing and manuring; intensity4 = 4-5 times mowing and manuring

Tillage: last time of tillage

Land.use.history: for Initial plots = recent agricultural use; for all restored sites = agricultural use before restoration
Mowing	earliest date of biomass harvesting (excluding “Initial”) based on contracts between farmers and the Nature Protection Agency of Zurich

Soil.type: based on soil mapping survey 1991 (before restoration)

Soil.texture: determined by the sediment method (Gee & Bauder 1986) and classified into soil textural classes according to FAO (1990)

pH: soil pH (CaCl2)

Slope: slope gradient classes according to FAO (1990; %)

en: Nematode colonizer-persister classes

en:
Plot: Plot ID combining treatment code and plot number (in total 55 plots)

Treatment: Three restoration treatments and two reference treatments. Initial=intensively managed grasslands; Harvest only= multiple hay harvest (2-3 times) per years; Topsoil= Topsoil removal (upper 10-30 cm); Topsoil+Propagules= Topsoil removal (upper 10-30 cm) combined with introduction of target plant species; Target= species-rich ancient grasslands

CP1.a: C-P (colonizer-persister) class 1 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990; equivalent to enrichment indicators according to Ferris, Bongers, & de Goede 2001
CP2.a: C-P (colonizer-persister) class 2 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990; equivalent to stress indicators according to Ferris, Bongers, & de Goede 2001
CP3.a: C-P (colonizer-persister) class 3 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990
CP4.a: C-P (colonizer-persister) class 4 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990
CP5.a: C-P (colonizer-persister) class 5 nematode abundance (individuals per 100g dry soil); C-P classification according to Bongers 1990

en: Nematode feeding types

en:
Plot: Plot ID combining treatment code and plot number (in total 55 plots)

Treatment: Three restoration treatments and two reference treatments. Initial=intensively managed grasslands; Harvest only= multiple hay harvest (2-3 times) per years; Topsoil= Topsoil removal (upper 10-30 cm); Topsoil+Propagules= Topsoil removal (upper 10-30 cm) combined with introduction of target plant species; Target= species-rich ancient grasslands

He.ecto.a: Herbivorous ectoparasite nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

He.endo.a: Herbivorous endoparasite nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Ba.a: Bacterivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Fu.a: Fungivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Om.a: Omnivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

Ca.a: Carnivorous nematode abundance (individuals per 100g dry soil); classification according to Sieriebriennikov, Ferris, & de Goede, 2014

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:
 > Resch, M.C., Schütz, M., Graf, U., Wagenaar, R., van der Putten, W.H., Risch, A.C. 2019. Does topsoil removal in grassland restoration benefit both soil nematode and plant communities? Journal of Applied Ecology 56: 1782-1793.

Please cite this paper together with the citation for the datafile.

 Methods
 Study area and experimental settings
The study was conducted in a nature reserve (Eigental: 47° 27’ to 47° 29’ N, 8° 37’ E, 461 to 507 m a.s.l.) that is located on the Swiss Central plateau close to Zurich airport (Canton Zurich, Switzerland). The mean annual temperature in this area ranges from 8.9 to 10.6 °C, mean annual precipitation from 910 to 1260 mm [10-year average (2007-2017); MeteoSchweiz, 2018]. The main soil types are calcaric to gleyic Cambisol and Gleysols. The reserve was established in 1967 to protect small remnants of oligotrophic semi-natural grasslands (roughly 12 ha). The plant community can be characterized as Molinion and Mesobromion (semi-wet to semi-dry), depending on the site-specific groundwater level and slope inclination (Delarze, Gonseth, Eggenberg, & Vust, 2015). These remnants represent species-rich islands in an otherwise intensively managed agricultural landscape. Semi-natural grasslands covered an area of 60,000 ha in the Canton Zurich in 1939, however, by 2005 only roughly 600 ha remained (Baudirektion Kanton Zürich, 2007). In 1990, the government of Canton Zurich decided to enlarge the nature reserve Eigental. The goal was to incorporate eleven patches of 20 ha adjacent intensively farmed land and transform these patches into semi-natural grasslands. The patches had a different agricultural history, ranging from permanent (no tillage for >50 years) to temporary grassland (as part of crop rotation; last tillage <5 years). On all freshly integrated patches fertilization was stopped in 1992 and from then on biomass was harvested three times a year and removed. After 5 years without noticeable effects on vegetation composition, the Nature Conservation Agency of Canton Zurich decided to increase the restoration efforts. In 1995, a large-scale experiment was initialized to evaluate if certain treatments can facilitate restoration within a reasonable timeframe of 5 to 10 years after treatment implementation.
The three restoration treatments used were:
i. “Harvest only”: Plots are being mowed two to three times a year and the biomass is removed.
ii. “Topsoil”: Topsoil was removed to a depth of 10 to 20 cm, depending on the depth of the O and A horizon, in four randomly selected areas within each of the eleven patches in late autumn 1995. The size of each topsoil removal area depended on individual patch size and was between 2700 and 7000 m2.
iii. “Topsoil+Propagules”: Propagules from target vegetation were added on half of the area where topsoil was removed, using fresh, seed-containing hay originating from a mixture of semi-dry to semi-wet species-rich grasslands of local provenance (within a radius of 7 km). Hay applications were conducted twice in 1995 and 1996. Repeated applications were chosen to account for the low quantity of available plant material per transfer, since area ratio between receptor and donor sites was roughly 1:1. In addition, hand-collected propagules from 15 selected target species of regional provenance (within a radius of 30 km) were equally applied in 1996 and 1997.
“Topsoil” and “Topsoil+Propagules” plots are mowed once a year, and the biomass is removed. Mowing on these plots started five years after the treatment was implemented.
Eleven permanent plots of 5 m x 5 m were randomly established in each treatment to monitor the vegetation development. The experiment was complemented with 11 control plots that represent the initial state of intensively managed grasslands, further referred to as “Initial”, and 11 control plots that represent the targeted state of donor sites for “Topsoil+Propagules”, further referred to as “Target”. Consequently, the experiment consists of 55 plots (5 treatments x 11 replicates). Management of intensively used grasslands includes mowing and fertilizing (manure) between two to five times a year, as well as different tillage regimes (no tillage for >50 years; last time of tillage <5 years).

 Nematode and plant sampling
Soil nematodes were sampled in 2 m x 2 m plots, randomly established at least 2 m away from the vegetation plots. We collected eight soil cores with a 2.2 cm diameter soil core sampler (Giddings Machine Company, Windsor, CO, USA) to a depth of 12 cm (representing the majority of the plant rooting system) in each plot at the beginning of July 2017. The eight cores within each replicate plot were combined, gently homogenized, placed in coolers and transported to the laboratory of NIOO in Wageningen, the Netherlands, within one week. Free-living nematodes were extracted from 200 g of fresh soil using Oostenbrink elutriator (Oostenbrink, 1960) and concentrated, resulting in 6 mL nematode solution. The nematode solution was subdivided into three subsamples, two for morphological identification and quantification, and one for molecular work (not used in this study). For morphological identification and quantification, nematodes were heat-killed at 90 °C and fixed in 4 % formaldehyde solution (final volume 10 mL per subsample). All nematodes in 1 mL of formaldehyde solution were counted, and a minimum of 150 individuals per 1 mL sample (or all if less nematodes were present) were identified to family level using Bongers (1988). We then extrapolated the numbers of each nematode taxa identified to the entire sample and expressed them per 100 g dry soil for further analyses.
We calculated number of nematode taxa and Shannon diversity and assessed nematode community composition. In addition, we classified the nematode taxa into feeding types (herbivores, bacterivores, fungivores, omni-carnivores), structural and functional guilds (Table S4). Structural guilds assign nematode taxa according to life-history traits into five colonizer-persister (C-P) classes, ranging from one (early colonizers of new resources) to five (persisters in undisturbed habitats; Bongers 1990). C-P classes can be categorized as indicators for nutrient-enriched (C-P1), stressed (C-P2) and structured (C-P3 + C-P4 + C-P5) soil conditions (Ferris, Bongers, & de Goede, 2001). Functional guilds assign nematode taxa according to their C-P classification combined with their feeding habits (Ferris, Bongers, & de Goede, 2001). Based on the structural and functional guild classification we calculated five additional indices to assess soil nutrient status, disturbance and food web characteristics using NINJA (Sieriebriennikov, Ferris, & de Goede, 2014). 1) The Maturity index indicates the degree of different environmental perturbations (e.g., tillage, nutrient enrichment, pollution) and is used to monitor colonization and subsequent succession after disturbances (Bongers, 1990). 2) The ratio between the Plant Parasite (C-P of herbivorous nematodes only) to Maturity index is used to monitor the recovery of disturbed habitats incorporating information of life-history traits for all feeding types (Bongers, van der Meulen, & Korthals, 1997). 3) The Enrichment index indicates nutrient-enriched soils and agricultural management practices (Ferris, Bongers, & de Goede, 2001). 4) The Structure index provides information about the succession stage of the soil food web and therefore correlates with the degree of maturity of an ecosystem (Ferris, Bongers, & de Goede, 2001). 5) The Channel index provides information about the predominant decomposition pathways, where higher values stand for a higher proportion of energy transformed through the slow fungal decomposition channel (Ferris, Bongers, & de Goede, 2001). In addition, the Structure and Enrichment indices can be displayed in a biplot where nematode assemblages are plotted along a structure (x-axis) and enrichment (y-axis) trajectory (increasing index values). Each biplot quadrat reflects different levels of disturbance, soil nutrient pools and decomposition pathways (Ferris, Bongers, & de Goede, 2001).
The plant surveys were conducted on the 25 m2 permanent plots in June 2017. Plant species cover was visually assessed according to the semi-quantitative cover-abundance scale of Braun-Blanquet (1964; nomenclature: Lauber & Wagner, 1996). We calculated number of species and Shannon diversity, and assessed plant community composition. We also counted the number of target species (all species recorded in the eleven target plots plus propagules of species applied by hand, resulting in a total of 143 species) and categorized plant species into species of concern based on their red list status in Switzerland as well as their protection status in Switzerland and the Canton Zurich (Moser, Gygax, Bäumler, Wyler, & Palese, 2002). Furthermore, we calculated indicator values for soil moisture and soil nutrients for each species according to Landolt et al. (2010).

 References
Baudirektion Kanton Zürich (2007). 10 Jahre Naturschutz-Gesamtkonzept für den Kanton Zürich 1995-2005 – Stand der Umsetzung. Zürich: Baudirektion Kanton Zürich.
Bongers, T. (1988). De nematoden van Nederland. Utrecht: Stichting Uitgeverij Koninklijke Nederlandse Natuurhistorische Vereniging.
Bongers, T. (1990). The maturity index: an ecological measure of environmental disturbance based on nematode species composition. Oecologia, 83, 14-19. doi:10.1007/BF00324627
Bongers, T., van der Meulen, H., & Korthals, G. (1997). Inverse relationship between the nematode maturity index and plant parasite index under enriched nutrient conditions. Applied Soil Ecology, 6, 195-199. doi:10.1016/S0929-1393(96)00136-9
Braun-Blanquet, J. (1964). Pflanzensoziologie, Grundzüge der Vegetationskunde (3rd ed.). Wien: Springer.
Delarze, R., Gonseth, Y., Eggenberg, S., & Vust, M. (2015). Lebensräume der Schweiz: Ökologie - Gefährdung - Kennarten (3rd ed.). Bern: Ott.
Ferris, H., Bongers, T., & de Goede, R.G.M. (2001). A framework for soil food web diagnostics: extension of the nematode faunal analysis concept. Applied Soil Ecology, 18, 13-29. doi:10.1016/S0929-1393(01)00152-4
Landolt, E., Bäumler, B., Erhardt, A., Hegg, O., Klötzli, F., Lämmler, W., … Wohlgemuth, T. (2010). Flora indicativa. Ecological indicator values and biological attributes of the Flora of Switzerland and the Alps (2nd ed.). Bern: Haupt.
Lauber, K., & Wagner, G. (1996). Flora Helvetica. Flora der Schweiz. Bern: Haupt.
MeteoSchweiz (2018). Klimabulletin Jahr 2017, Zürich: MeteoSchweiz.
Moser, D., Gygax, A., Bäumler, B., Wyler, N., & Palese, R. (2002) Rote Liste der gefährteten Farn- und Blütenpflanzen der Schweiz. Bern: BUWAL.
Oostenbrink, M. (1960). Estimating nematode populations by some selected methods. In N.J. Sasser & W.R. Jenkins (Eds.), Nematology (pp. 85-101). Chapel Hill: University of North Carolina Press.
Sieriebriennikov, B., Ferris, H., & de Goede, R.G.M (2014). NINJA: An automated calculation system for nematode-based biological monitoring. European Journal of Soil Biology, 61, 90-93. doi:10.1016/j.ejsobi.2014.02.004

diversity

display_name: diversity

grassland

display_name: grassland

long-term-experiment

display_name: long-term-experiment

nematodes

display_name: nematodes

restoration

display_name: restoration

topsoil-removal

display_name: topsoil-removal

vegetation

display_name: vegetation

en: Grassland restoration: nematodes and plant communities


maintainer: EnviDat Support

en: par meteo long-term relative-humidity wind-speed precipitation temperature swiss-forest-lab climate-change meteorology

state: active

type: dataset

en: Data plot of the forest station (LAB)

media_type: application/pdf

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

format: PDF

mimetype: application/pdf

en: Site aerial photo (Lausanne)

en: Site aerial photo from both Lausanne stations. Source: Google. 2016. Site aerial photo from both Lausanne stations. Retrieved November 11, 2016, from https://www.google.ch/maps

format: SERVICE

mimetype: image/jpeg

en: Site photo of the field station (LAF)

en: Site photo of the field station from the side.

en: Data plot of the field station (LAF)

en: Field station data (LAF)

media_type: text/csv

en: Data file.

format: CSV

mimetype: text/csv

en: Site photo of the forest station (LAB)

en: Site photo of the fforest station from the side.

en: Forest station data (LAB)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Lausanne in Switzerland where one station is located within a natural mixed forest stand (LAB) with European beech (_Fagus sylvatica_; 160-170 yrs), European silver fir (_Abies alba_; 160-170 yrs) and Norway spruce (_Picea abies_; 160-170 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, LAF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Lausanne is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

educ

fr: Éducation, culture et sport

de: Bildung, Kultur und Sport

en: Education, culture and sport

it: Istruzione, cultura e sport

envidat

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

en: EnviDat: the Environmental Data Portal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: Lausanne, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1996 onwards


maintainer: Felix Neff

en: functional-diversity diversity grassland restoration long-term-experiment insects functional-traits topsoil-removal

en: Plot information

en:
Information on the experimental plots.

- **Plot**: ID of Plot
- **Treatment**: Experimental treatment
- **Landuse_history**: recent agricultural use for Initial; agricultural use before restoration for all restored sites
- **Last_tilled**: Last time the site was tilled
- **Intensity**: Frequency and intensity of mowing and manuring (*intensity1*: mowing once and no manure, i*ntensity2*: 2-3 times mowing and no manure, *intensity3*: 2-3 times mowing and manuring, *intensity4*: 4-5 times mowing and manuring)
- **Slope**: slope gradient according to classes defined by FAO (1990)
- **X**: X coordinate of the plot (CH1903)
- **Y**: Y coordinate of the plot (CH1903)
- **Cluster**: Spatial cluster of the plot

en: Insect abundance

en:
Abundance of adult insects recorded in the samplings on all experimental sites

- **Plot**: ID of Plot
- **Treatment**: Experimental treatment
- **Species**: Insect species name (following [Fauna Europaea](https://fauna-eu.org))
- **Author**: Author and year of species' description (following [Fauna Europaea](https://fauna-eu.org))
- **Group**: Insect group as used in the analyses
- **Number_Adults**: Number of adult individuals recorded
- **Herbivore**: Logical indicating whether the species is feeding as a herbivore (*TRUE*) or not (*FALSE*)

en: Insect traits

en:
Functional traits of all herbivorous insect species sampled in the study.

- **Species**: Insect species name (following [Fauna Europaea](https://fauna-eu.org))
- **Author**: Author and year of species' description (following [Fauna Europaea](https://fauna-eu.org))
- **Group**: Insect group as used in the analyses
- **Body volume**: Body volume determined by body length, body width and body height (in mm$^3$)
- **Body_shape**: Ratio of body length to body width
- **Hind_femur_shape**: Ratio of hind-femur length to hind-femur width
- **Hind_front_leg_ratio**: Ratio of front-leg length to hind-leg length (leg length = femur length + tibia length)
- **Wing_length_rel**: Ratio of wing length to body length
- **Leg_length_rel**: Ratio of front-leg length to body length (leg length = femur length + tibia length)
- **Antenna_length_rel**: Ratio antenna length to body length
- **Eye_width_rel**: Ratio of eye width to head width (Auchenorrhyncha, Heteroptera) / to head height (Orthoptera)
- **Feeding_specialization**: Insect specialization on food source (*m*: monophagous, *o*: oligophagous, *p*: polyphagous)
- **Feeding_tissue**: Feeding tissue used for feeding (*c*: chewers, *x*: xylem, *p*: phloem, *m*: mesophyll, *m-p/r*: mesophyll as well as phloem and/or reproductive organs, *r*: reproductive organs, *se*: ripe seeds)
- **Hibernation**: Hibernation stage (*e*: egg, *e/n*: egg and nymph, *n*: nymph, *a*: adult)
- **Generations**: Number of generations per year (*1.5* stands for *1-2* generations per year)
- **Moisture_pref_mean**: mean of preferred habitat moistures (*1*: xerophilous, *2*: moderately xerophilous, *3*: moderately mesohygrophilous, *4*: mesohygrophilous, *5*: moderately hygrophilous, *6*: hygrophilous, *7*: moderately hydrophilous, *8*: hydrophilous)
- **Moisture_pref_range**: range of preferred habitat moistures (indicating degree of specialization)

en:
This dataset contains all data, on which the following publication below is based.

**Paper Citation**:

Neff, F., Resch, M. C., Marty, A., Rolley, J. D., Schütz, M., Risch, A. C, Gossner, M. M. 2020. Long-term restoration success of insect herbivore communities in semi-natural grasslands: a functional approach. Ecological Applications, 30, e02133. [10.1002/eap.2133](https://doi.org/10.1002/eap.2133)

Please cite this paper together with the citation for the datafile.

 Methods

 Study site
The study area is situated within and nearby to Eigental nature reserve (47°27’36” to 47°29’06” N, 8°37’12” to 8°37’44” E, 461 to 507 m a.s.l.) in the vicinity of Zurich airport (Canton Zurich, Switzerland). Mean annual precipitation and temperature is 903 ± 136 mm and 9.14°C ± 0.50°C (mean ± SD for 2007-2017 (*MeteoSchweiz 2018*)). In 1967, the Eigental nature reserve was established to protect small and isolated remnants of species-rich, semi-natural grasslands (roughly 12 ha), which were embedded in an otherwise intensively managed landscape. It is characterized by oligo- to mesotrophic Molinion (semi-wet, matrix species *Molinia caerulea*) and Mesobromion (semi-dry, matrix species *Bromus erectus*) meadows (*Delarze et al. 2015*), reflecting small-scale habitat heterogeneity, mainly due to site-specific groundwater levels and slope inclination. As in most Central European grasslands, management is necessary to prevent shrub and tree invasions as well as to secure low levels of available soil nutrients and thus to maintain these species-rich habitats ([*Poschlod and WallisDeVries 2002*](https://doi.org/10.1016/S0006-3207(01)00201-4)).
In 1990, the government of the Canton Zurich decided to enlarge the Eigental nature reserve as a counter measure against degradation and biodiversity loss in semi-natural grasslands due to overutilization and the excessive input of nutrients (mostly nitrogen). Eleven patches of adjacent intensively managed grassland (in total roughly 20 ha) were targeted to be transformed into semi-natural grasslands. As a first restoration measure, fertilization was ceased, and biomass harvested three times to remove excessive soil nutrients from the original system and thus benefit plant species with low competitive ability on the long run. In 1995, the restoration efforts were increased and a large-scale experiment comprising three restoration measures with increasing intervention intensities was implemented:

- **Harvest only**: Initial restoration measures were continued with mowing and removing of the aboveground biomass two times a year (early summer and autumn).
- **Topsoil**: Removal of topsoil, depending on the thickness of the A horizon the upper 10 to 20 cm, in four randomly selected areas within the eleven patches in late autumn 1995. The size of the restoration area depended on individual patch size (2700 to 7000 m2).
- **Topsoil + Propagules**: Plant propagules were added on half of the area where topsoil was removed via application of fresh, seed-containing hay and hand-collected propagules of target species originating from semi-dry and semi-wet species-rich grasslands with local and regional provenance (within radius of 7 to 30 km) (1995, 1996, 1997).

Management of *Topsoil* and *Topsoil + Propagules* started five years after treatment implementation and included yearly mowing and removing of aboveground biomass (late summer or early autumn). The experiment was complemented with intensively managed grassland sites that share the same agricultural history as the restored sites (**Initial**; swards dominated by *Lolium perenne*, *L. multiflorum* and *Trifolium repens*): mowing and subsequent fertilizing (manure) up to five times a year, as well as different tillage regimes. Finally, sites were selected in target semi-dry and semi-wet grasslands (**Target**) located within the Eigental nature reserve and another nature reserve nearby (Altläufe der Glatt; 47°28’29” to 47°27’41” N, 8°31’56” to 8°32’26” E, 418 to 420 m a.s.l.). The selected target sites are mown and aboveground biomass removed once a year in late summer or early autumn. For each of the five treatments, we selected eleven plots (5 m × 5 m) spread across the sites. Altogether, the experiment included 55 plots.

 Arthropod sampling
Aboveground arthropods were sampled using suction sampling on four consecutive days in early July 2017 before the grasslands were mown. Arthropods were sampled in two locations on each 5 m × 5 m plot, once in the south-western and once in the north-eastern corner to account for possible spatial heterogeneity within the plots. Arthropods were sorted to order or lower taxonomic levels and individuals were identified to species level. We focused on three groups (Hemiptera: Auchenorrhyncha, Hemiptera: Heteroptera, Orthoptera),

 Functional traits
We used two sets of functional traits in this study.

**Morphometric traits**:
Body volume, body shape, hind femur shape, hind/front leg ratio, wing length, leg length, antenna length and eye width. We used trait measurements from [*Simons et al. (2016)*](http://dx.doi.org/10.1890/15-0616.1) and [*Neff et al. (2019)*](https://doi.org/10.1007/s10980-019-00872-1) and complemented them with measurements on study specimens. These measurements were conducted using a high-resolution measuring stereo microscope (Leica DVM6, Leica Microsystems) including automated high-resolution photo stacking with the software Leica Application Suite X (LAS X, © 2018 Leica Microsystems CMS GmbH) and Leica Map Premium (Leica Microsystems, © 1996-2017 Digital Surf) at WSL Birmensdorf. The eight morphometric traits were calculated from direct measurements of body parts on specimens of all sampled species. From each species, we measured at least one female and one male specimen. Additionally, for species that show wing dimorphism, we included the different wing morphs and weighted them by their prevalence reported in literature. For few species, of which not all wing morphs were available for measurements (10 cases), we estimated relative wing length from congeneric species or from the literature.

**Life-history traits**
Based on an existing data set collected by [*Gossner et al. (2015)*](http://dx.doi.org/10.1890/14-2159.1). We included traits describing different life-history characteristics of herbivore insect species, namely: feeding specialization, feeding tissue, hibernation stage and number of generations per year, which are related to insect species’ vulnerability to changes in plant community composition, microhabitat use and disturbance tolerance. To represent potential changes in habitat moisture with abandonment of intensive land use (e.g., change in ground-water level), we also included two traits related to preferred habitat moisture of the study species: moisture preference, describing species’ optimum habitat moisture, and moisture range, which describes the species’ range of preferable moisture conditions.

 References

Delarze, R., Y. Gonseth, S. Eggenberg, and M. Vust. 2015. Lebensräume der Schweiz: Ökologie - Gefährdung - Kennarten. 3rd ed. Ott, Bern.

Gossner, M. M., N. K. Simons, R. Achtziger, T. Blick, W. H. O. Dorow, F. Dziock, F. Köhler, W. Rabitsch, and W. W. Weisser. 2015. A summary of eight traits of Coleoptera, Hemiptera, Orthoptera and Araneae, occurring in grasslands in Germany. Scientific Data 2:150013.

MeteoSchweiz. 2018. Klimabulletin Jahr 2017. MeteoSchweiz, Zürich.

Neff, F., N. Blüthgen, M. N. Chisté, N. K. Simons, J. Steckel, W. W. Weisser, C. Westphal, L. Pellissier, and M. M. Gossner. 2019. Cross-scale effects of land use on the functional composition of herbivorous insect communities. Landscape Ecology 34:2001–2015.

Poschlod, P., and M. F. WallisDeVries. 2002. The historical and socioeconomic perspective of calcareous grasslands—lessons from the distant and recent past. Biological Conservation 104:361–376.

Simons, N. K., W. W. Weisser, and M. M. Gossner. 2016. Multi-taxa approach shows consistent shifts in arthropod functional traits along grassland land-use intensity gradient. Ecology 97:754–764.

diversity

display_name: diversity

functional-diversity

display_name: functional-diversity

functional-traits

display_name: functional-traits

insects

display_name: insects

en: Grassland restoration: insects and insect traits


data-broedlin-cnp

title_for_slug: data-broedlin-cnp

en: carbon soil drying-rewetting nitrogen dissolved-organic-matter forest phosphorus stoichiometry mineralization

en: Data Broedlin-CNP-Release

en: Raw data of C-N-P mobilization experiment from Oi, Oe/Oa, and A horizons of beech forest soils along a nutrient availability gradient.

format: XLS

en: Data Broedlin-CNP

en: Mircocosm experiment to identify the individual patterns and controls of C, N, and P mobilization in soils under beech forests. Organic and mineral horizons sampled along a nutrient availability gradient in Germany were exposed to either permanent moist conditions or to dry spells in microcosms and quantified the release of inorganic and organic C, N, and P.

carbon

display_name: carbon

dissolved-organic-matter

display_name: dissolved-organic-matter

drying-rewetting

display_name: drying-rewetting

forest

display_name: forest

mineralization

display_name: mineralization

nitrogen

display_name: nitrogen

phosphorus

display_name: phosphorus

soil

display_name: soil

stoichiometry

display_name: stoichiometry

en: Data Broedlin CNP


maintainer: Armin Sigmund

en: antarctica evaporation isotopic-distillation snow-sublimation backward-trajectories lagrangian-analysis stable-water-isotopes

en: Readme

media_type: text/plain

en: Meta data

format: TXT

en: Data

en: Modeled data including the data shown in the figures of the related research article

en: Python scripts for data visualization

media_type: application/zip

en: Scripts for generating the figures of the related research article and generating the data files (csv) from the model output

format: ZIP

en: Model code

en: Model Sublimation and Model Air Parcel (python scripts)

en:
 Summary

This data set contains Python programming code and modeled data discussed in a related research article. We developed a simple isotope model to study the drivers of the particularly depleted vapour isotopic composition measured on the ship of the Antarctic Circumnavigation Expedition close to the outlet of the Mertz glacier, East Antarctica, in the 6-day period from 27 January 2017 to 1 February 2017. The model considers the stable water isotopologues H2(16O), H2(18O), and HD(16O). It uses data from the ERA5 reanalysis product with a spatial resolution of 0.25° x 0.25° (Hersbach et al., 2018) and 10-day backward trajectories for the location of the ship, published by Thurnherr et al. (2020a). Our data set includes the model code, Python scripts for visualizing the results, and data produced by the model including the results shown in the figures of the related research article. Here, we summarize the most important model characteristics while further details can be found in the readme.txt file and the related research article including its supporting information.

 Main model characteristics

The modeling approach consists of two steps called *Model Sublimation* and *Model Air Parcel*. The former estimates the isotopic compositions of the snow and sublimation flux across the Antarctic Ice Sheet using an Eulerian frame of reference while the latter models the vapour isotopic composition and specific humidity along air parcel trajectories using a Lagrangian frame of reference. The isotope effects of most phase changes are represented by equilibrium fractionation. Only for ocean evaporation, kinetic fractionation is additionally taken into account (original Craig-Gordon formula). For snow sublimation, two assumptions are tested: *Run E* assumes that sublimation is associated with equilibrium fractionation while *Run N* assumes that sublimation occurs without isotopic fractionation.

 Model Sublimation
Model Sublimation uses a simple one-dimensional mass-balance approach in each grid cell, considering snow accumulation due to snowfall and vapour deposition and snow ablation due to sublimation. The snowpack is represented by 100 layers of equal thickness (e.g., 1 cm) and density (350 kg m-3). The isotopic composition of snowfall is parameterized by generalizing a site-specific, empirical relationship between the daily mean air temperature and snowfall isotopic composition. In the case of vapour deposition, Model Sublimation assumes equilibrium fractionation and estimates the isotopic composition of the atmospheric vapour as the average value for two idealized situations: (i) locally sourced vapour which has the same isotopic composition as the sublimation flux; (ii) non-locally sourced vapour in isotopic equilibrium with snowfall. Model Sublimation is run with a time step of 1 h, independently of Model Air Parcel.

 Model Air Parcel
Every hour, an ensemble of trajectories arrives at different heights in the ABL above the ship. For each of these trajectories, we consider an air parcel with a constant volume of 1 x 1 x 1 m3. The air parcels are initialized at the first suitable time when the trajectories are located in the ABL, either over the ice-free ocean in conditions of evaporation or over snow (Antarctic Ice Sheet or sea ice). Subsequently, the masses of the water isotopologues in the air parcels are simulated with a time step of 3 h, considering vapour uptake or removal due to the moisture flux at the snow or liquid ocean surface (only if the parcel is in the ABL) and cloud/precipitation formation (if the saturation specific humidity is reached). Sea ice is taken into account in a very simplified way. We represent the sea ice by grid cells with a sea-ice cover of more than 90% and assume the isotopic composition of the sublimation flux to be identical to that in the nearest grid cell of the Antarctic Ice Sheet. The isotopic composition of the sublimation flux is taken from Model Sublimation whereas the isotopic composition of the vapour deposition flux (over snow) and condensation flux (over ice-free ocean) is simulated assuming an isotopic equilibrium with the air parcel. Isotope effects of cloud/precipitation formation are represented using the classic Rayleigh distillation model with equilibrium fractionation, where the cloud water is assumed to precipitate immediately after formation.

 References

Hersbach, H., Bell, B., Berrisford, P., Biavati, G., Horanyi, A., Munoz Sabater, J.,... others (2018). *ERA5 hourly data on single levels from 1979 to present. Copernicus Climate Change Service (C3S) Climate Data Store (CDS)*. doi: 10.24381/cds.bd0915c6

Thurnherr, I., Wernli, H., & Aemisegger, F. (2020a). *10-day backward trajectories from ECMWF analysis data along the ship track of the Antarctic Circumnavigation Expedition in austral summer 2016/2017*. Zenodo. doi: 10.5281/zenodo.4031705

antarctica

display_name: antarctica

backward-trajectories

display_name: backward-trajectories

evaporation

display_name: evaporation

isotopic-distillation

display_name: isotopic-distillation

lagrangian-analysis

display_name: lagrangian-analysis

snow-sublimation

display_name: snow-sublimation

stable-water-isotopes

display_name: stable-water-isotopes

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Modeled Isotopic Composition of Water Vapour Along Air Parcel Trajectories in the Antarctic


maintainer: Christian G. Sommer

en: wind-crust kinect wind-tunnel snow wind-packing

en: Processing scripts

en: Contains the Matlab- and R-scripts used to process the Kinect data

mimetype: application/zip

en: README

en: Describes the dataset in detail

mimetype: text/plain

en: Kinect data

en: Links to the dataset

en:
This data sets contains the Microsoft Kinect data from 15 wind tunnel experiments on wind-packing / wind crust formation. These experiments were performed in the winter 2016/17. The Kinect measures distributed snow depth.

The Kinect data is organized per experiment. Each experiment subfolder contains the processed Kinect depth images and some additional files.

Please refer to the README for more details on the data. The processing scripts are available for download as well. The scripts are mainly provided as documentation and would need to be adjusted to be used.

This dataset is the basis of the following publication:

Sommer C.G., Lehning M. & Fierz C. (2018). Wind Tunnel Experiments: Influence of Erosion and Deposition on Wind-Packing of New Snow. Front. Earth Sci. 6:4. doi: 10.3389/feart.2018.00004

kinect

display_name: kinect

snow

display_name: snow

wind-crust

display_name: wind-crust

wind-packing

display_name: wind-packing

wind-tunnel

display_name: wind-tunnel

en: Wind crust formation: Microsoft Kinect data


en: switzerland ecology environmental--policy environmental---history historical-carthography wetlands

en: Wetlands 1850 - 2010 (shapefiles)

en:
 History of wetlands - GIS-Layers
 Location of wetlands based on signatures in topographic maps
* Basis 1850: original measuring table sheets Dufour created between the years 1834 and 1863.
* Basis 1900/ Basis 1950: The Siegfried maps were updated and published at regular intervals between 1870 and 1949. This long period of time made it possible to reconstruct wetlands for two periods based on the Siegfried Maps: the first edition (hereinafter referred to as "1900") and the last edition ("1950").
* Basis 2010: Map sheets from the National Map from 2006 to 2011 were used for the most recent time period of the reconstruction.
 Location of wetlands due to reconstructions
The reconstruction **must not** be interpreted in the sense of a surface-accurate separation, but shows without claim to location accuracy the approximate extent of the areas that would have corresponded to the mapping criteria for wetlands used in today's topographic maps around 1850, 1900 and 1950. The basics can be found in Stuber & Bürgi 2019, chapter 5.4.

en:
Naturally, large parts of the Swiss Plateau are characterised by wetlands
and meandering rivers. That this is no longer the case today is the result
of centuries of efforts to obtain dry land. But how did this process take
place? What were the relevant actors and what were their motivations? And
what can be said about the ecological consequences of this development?

In a research project on the history of wetlands in Switzerland since 1700,
we conducted (a) a historical analysis of the development of land use in
wetlands and the actors involved, (b) a historical-cartographic
reconstruction of wetland extent since 1850 and (c) an evaluation of
ecological effects of changes in wetlands on various organisms groups.

The series of GIS layers on wetland history stem from the second part of
the project. The area reconstruction is based on digitized and homogenized
signatures from national map series, as they have been available since
about 1850. Details about the digitalization process and the homogenization
procedures applied ("Rekonstruktionen") are included in Stuber & Bürgi
2019.

Book Citation:
 > Stuber M, Bürgi M (2019) Vom «eroberten Land» zum Renaturierungsprojekt. Geschichte der Feuchtgebiete in der Schweiz seit 1700. "Bristol Schriftenreihe", Band 59. Haupt Verlag, Bern, Stuttgart, Wien. 262 Seiten.

ecology

display_name: ecology

environmental---history

display_name: environmental---history

environmental--policy

display_name: environmental--policy

historical-carthography

display_name: historical-carthography

switzerland

display_name: switzerland

wetlands

display_name: wetlands

en: History of wetlands in Switzerland since 1850


maintainer: Andrin Caviezel

en: rockfall-experiments rockfall stonenodedata induced-rockfall rockfall-runout sensor-stream deposition-points stonenode natural-hazards dem

en: Induced Rockfall Data, Tschamut, GR

en:
 Induced Rockfall Experimental Data, Tschamut, GR, Switzerland

The archive contains site specific geographical data as well as the deposition points of manually induced rockfall by releasing differently shaped boulders with 30–80 kg of mass. Additionally availabe are the StoneNode v1.0 sensor streams for the equipped rocks.

The _OverviewFigures_ file serves as quick representation of the included data. It also contains the overview table for the rock masses in order to set the dimensions accordingly in RAMMS::ROCKFALL.

The archive contains the following folders:
<\ul>
* DEM: ArcGIS compatible DEM Tschamut_DEM_2m (grid resolution of 2 m) as well as the .asc grid used in RAMMS::ROCKFALL simulations
* _DepositionPoints_: containing two folders _RF Tschamut Frozen_ and _RF Tschamut WET_ containing each a shapefile with the complete deposition data of each series as well as deposition shapfiles for each rock shape.
* _DigitizedRocks_: Digitized point clouds of the used rocks as used for RAMMS::ROCKFALL simulations (.pts files) (dimensions have to be checked).
* _Orthofoto_: Orthophoto of the experimental site obtained via UAV with 5 cm resolution.

en: StoneNode v1.0 Data for equipped experimental Runs.

en: Corresponding StoneNode v1.0 data streams belonging to the deposition points.

en:
 Dataset of an experimental campaign of induced rockfall in Tschamut, Grisons, Switzerland.

The data archive contains site specific geographical data such as DEM and orthophoto as well as the deposition points of manually induced rockfall by releasing differently shaped boulders with 30–80 kg of mass. Additionally available are all the StoneNode data streams for rocks equipped with a sensor. The data set consists of

* Deposition points from two series (wet (27/10/2016) and frozen (08/12/2016) ground)
* Digital Elevation Model (grid resolution 2 m) obtained via UAV
* Orthophoto (5 cm resolution) obtained via UAV
* Digitized rock point clouds (.pts input files for RAMMS::ROCKFALL)
* StoneNode v1.0 raw data stream for equipped rocks.

Further information is found in

* A. Caviezel et al., _Design and Evaluation of a Low-Power Sensor Device for Induced Rockfall Experiments_, IEEE Transactions on Instrumentation and Measurement, 2018, 67, 767-779, http://ieeexplore.ieee.org/document/8122020/
* P. Niklaus et al., _StoneNode: A low-power sensor device for induced rockfall experiments_, 2017 IEEE Sensors Applications Symposium (SAS), 2017, 1-6, http://ieeexplore.ieee.org/document/7894081/

dem

display_name: dem

deposition-points

display_name: deposition-points

induced-rockfall

display_name: induced-rockfall

natural-hazards

display_name: natural-hazards

rockfall

display_name: rockfall

rockfall-experiments

display_name: rockfall-experiments

rockfall-runout

display_name: rockfall-runout

sensor-stream

display_name: sensor-stream

stonenode

display_name: stonenode

stonenodedata

display_name: stonenodedata

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: Induced Rockfall Dataset (Small Rock Experimental Campaign), Tschamut, Grisons, Switzerland


photogrammetric-drone-data-dorfberg

maintainer: Yves Bühler

title_for_slug: photogrammetric-drone-data-dorfberg

en: uav drone-data snow photogrammetry snow-depth-mapping dsm snow-distribution orthophoto

en: Data Access

en:
- 3cm-resolution Orthophoto
- 10cm-resolution DSM
- 10cm-resolution snow depth raster
- original RGB images
- Agisoft Metashape Report

All products are also available as a cloud optimized geotiff.

en: The data was collected with a Wingtra Gen II drone and a Sony RX1R II sensor. In total, 10 flights were conducted at different dates, both in summer and winter. A DSM, an orthophoto, a snow depth raster and the original drone images from every flight are available at a high resolution (10cm and 3cm, respectively).

drone-data

display_name: drone-data

dsm

display_name: dsm

orthophoto

display_name: orthophoto

photogrammetry

display_name: photogrammetry

snow

display_name: snow

snow-depth-mapping

display_name: snow-depth-mapping

snow-distribution

display_name: snow-distribution

uav

display_name: uav

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Photogrammetric Drone Data Dorfberg


maintainer: Ionut Iosifescu Enescu

en: open-data open-science reproducibility open-source jupyter-notebooks hpc research-data-management

en: Open Science Support @ WSL (Poster)

en:
This poster was originally created for the swissuniversities Open Science Action Plan: Kick-Off Forum, and showed to the audience on 17.10.2019. It illustrates how the environmental data portal EnviDat provides the tools for fostering Open Science and Reproducibility of scientific research at WSL.

Supporting open science is a highly relevant user requirement for EnviDat and for implementing FAIR (Findability, Accessibility, Interoperability and Reusability) principles at dataset level.
EnviDat encourages WSL scientists to complement data publication with a complete description of research methods and the inclusion of the open source software, code or scripts used for processing the dataset or for obtaining the published results. By openly publishing open software (e.g. as Jupyter notebooks) alongside research data sets, researchers can contribute to mitigate reproducibility issues.

EnviDat also promotes and supports, where possible and practical, the publication of software as Jupyter notebooks. Jupyter notebooks provide a solution for improved documentation and interactive execution of open code in a wide range of programming languages (Python, R, Octave/Matlab, Java or Scala). These programming languages are widely used in environmental research at WSL and well supported by the Jupyter-compatible kernels. We have sucessfully interfaced EnviDat-hosted notebooks with the WSL High-Performance Computing (HPC) Linux Cluster through a JupyterHub/JuypterLab beta installation on the HPC cluster implemented in close collaboration with the WSL IT-Services.
For existing software that cannot be easily migrated to Jupyter Notebooks, the Open Science and Reproducibility is assisted by containerisation. We have proven that several Singularity containers can successfully run on WSL's HPC cluster.

Finally, the researchers can upload the data/results complemented by code (e.g. as Jupyter Notebooks, or Singularity containers) and any additional documentation in EnviDat. Consequently, they will receive a DOI for the entire dataset, which they can reference in their science paper in order to publish a more reproducible research.

_License_: This poster is released by WSL and the EnviDat team to the public domain under a Creative Commons 4.0 CC0 "No Rights Reserved" international license. You can reuse this poster in any way you want, for any purposes and without restrictions.

hpc

display_name: hpc

jupyter-notebooks

display_name: jupyter-notebooks

open-data

display_name: open-data

open-science

display_name: open-science

open-source

display_name: open-source

reproducibility

display_name: reproducibility

research-data-management

display_name: research-data-management

en: Open Science Support at the Swiss Federal Research Institute WSL. The EnviDat Concept


maintainer: Albrecht von Boetticher

en: murgang hangmuren finite-volumes barrier-impact-simulation interfoam natural-hazard debris-flow dberisintermixing shallow-landslide

en: Installation Instructions for debrisInterMixing-6

en: These are the installation instructions for debrisInterMixing4

en: debrisInterMixing for OF-Version 6

en:
This version of debrisInterMixing is based on OpenFOAM Version 4.x and was tested on Ubuntu 18.04.1 LTS.
Please follow the instructions given in ReadMe_debrisInterMixing4.pdf for installation.

en:
Here the updated versions of debrisInterMixing are provided for download. The first OpenFoam-compatible Version 2.3.x are available as supplement to v. Boetticher, A., Turowski, J. M., McArdell,W. B., Rickenmann, D., Hürlimann, M., Scheidl, C., and Kirchner, J. W.: DebrisInterMixing-2.3: A Finite Volume solver for three dimensional debris flow simulations based on two calibration parameters. Part two: model validation with experiments. Geoscientific Model Development, 10, 11: 3963-3978. doi: 10.5194/gmd-10-3963-2017.
DebrisInterMixing is a Volume-of-Fluid based Finite Volume code that accounts for shear-thinning sensitive shares of fine sediment suspension together with pressure-sensitive components of the gravel grains within debris flow mixtures. All model properties can be derived from a material sample except for a grid-sensitive calibration parameter.
For more information, please contact albrecht.vonboetticher@wasserbau.ch.

barrier-impact-simulation

display_name: barrier-impact-simulation

dberisintermixing

display_name: dberisintermixing

debris-flow

display_name: debris-flow

finite-volumes

display_name: finite-volumes

hangmuren

display_name: hangmuren

interfoam

display_name: interfoam

murgang

display_name: murgang

natural-hazard

display_name: natural-hazard

shallow-landslide

display_name: shallow-landslide

en: Three-dimensional debris flow simulation tool debrisInterMixing


high-resolution-climate-data-for-europe

title_for_slug: high-resolution-climate-data-for-europe

en: cordex europe maximum-temperature precipitation minimum-temperature air-temperature daily-climate eur11

en: CHELSA EUR11 Technical Documentation

en: As the CHELSA project is continuously expanded, certain new parameters, time scales etc. are not included anymore in the original publication (Karger et al. 2017). We are therefore provide this document as a guideline of the current climatic parameters available and will expand it as new parameters or time periods will become available. Please refer to the version history for changes made in the document.

en:
High-resolution information on climatic conditions is essential to many applications in environmental and ecological sciences. Here we present downscaled climate data for the CORDEX EUR11 domain at a high resolution of 30 arc sec. The temperature algorithm is based on statistical downscaling of atmospheric temperature lapse rates. The precipitation algorithm incorporates orographic predictors including wind fields, valley exposition, and boundary layer height. The resulting data consist of a daily temperature and precipitation timeseries.

The data is distributed under a: Creative Commons: Attribution 4.0 International (CC BY 4.0) license.

air-temperature

display_name: air-temperature

cordex

display_name: cordex

daily-climate

display_name: daily-climate

europe

display_name: europe

maximum-temperature

display_name: maximum-temperature

minimum-temperature

display_name: minimum-temperature

precipitation

display_name: precipitation

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: High resolution climate data for Europe


en: shortwave-radiation photovoltaic snow winter-energy-production albedo

en: Data Forward Scattering

en: GROUNDEYE model for multiple forward scattering in snow-covered terrain. Meteorological and topographical input data set for the Meierhofertäli near Davos, Switzerland, September 2017 - August 2018. Output data for September 2017 - August 2018 at hourly resolution and every pixel. Post-processing scripts for plotting and analysis.

en:
 Overview
We present GROUNDEYE, a new model of radiative transfer over mountainous terrain, which considers for the first time the forward scattering properties of snow.
Embedded in the surface process model Alpine3D, the new terrain radiation model GROUNDEYE receives interpolated real weather data with diffuse and direct broadband shortwave radiation for each pixel as well as a spatially variable plane albedo from the module SNOWPACK.

 Format
The GROUNDEYE model is written in c++, as is the entire environment of Alpine3d. The input and output data sets are .xlsx or .txt format, pre- and postprocessing including the generation of all figures is in .R format.

 Structure
In Data_Forward_Scattering.zip you will find all necessary data and model details to
reproduce the results of the JGR publication "How forward-scattering snow and terrain change the Alpine radiation balance with application to solar panels"

 - 	Model Input Data contains the meteorological and topographic input data sets, the BRDF, and preprocessing scripts.
 - Model Code contains the full model Alpine3d including the radiative transfer module GROUNDEYE.
 - Model Output Data contains the results of the simulation of terrain irradiance and irradiance of solar panels; hourly resolution, 1. Sptember 2017 - 31. August 2018.
 - Measurements Solar Testsite contains information and measurements of the solar testsite at the Totalp near Davos, Switzerland.
 - Postprocessing contains all R-Scripts used for the analysis and
plotting of the corresponding data.

In each of these folders you will find detailed information in
the file 'About this Folder.txt'.

albedo

display_name: albedo

photovoltaic

display_name: photovoltaic

shortwave-radiation

display_name: shortwave-radiation

snow

display_name: snow

winter-energy-production

display_name: winter-energy-production

envidat

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Data, Code Link and Metadata on Forward Scattering of Snow at Totalp


snow-drift-station-micro-rain-radar

title_for_slug: snow-drift-station-micro-rain-radar

en: snow mountain raclets radar rain precipitation

en: MRR data of period 3

en: The dataset contains measurements between March 8 and March 13, 2019, with time in UTC. The parameters and specifications are given in MRR_parameters.pdf. The zip folder contains hourly data files in netCDF format and a log file, reporting the instrument status.

en: Parameter MRR

en: The PDF contains additional information on parameters which were set for the certain time periods (time in UTC).

en: MRR data of period 4

en: The dataset contains measurements between March 13 and March 22, 2019, with time in UTC. The parameters and specifications are given in MRR_parameters.pdf. The zip folder contains hourly data files in netCDF format and a log file, reporting the instrument status.

en: MRR data Period 2

en: The dataset contains measurements between March 5 and March 8, 2019, with time in UTC. The parameters and specifications are given in MRR_parameters.pdf. The zip folder contains hourly data files in netCDF format and a log file, reporting the instrument status.

en: MMR Data Period 1

en: The dataset contains measurements between March 1 and March 5, 2019, with time in UTC. The parameters and specifications are given in MRR_parameters.pdf. The zip folder contains hourly data files in netCDF format and a log file, reporting the instrument status.

en: The instrument (MRR, Metek) was mounted at Gotschnagrat (LON: 46.859 LAT: 9.849) at a height of 1 m above snow surface (at the beginning of the campaign) with an orientation of 22° with respect to North and a horizontal viewing direction. The sampling time was either 5 s or 10 s, depending on the settings at the specific period. The MRR produces standard outputs like radar reflectivity, doppler velocity, etc., and additional information can be found [here](https://metek.de/de/product/mrr-2/).

mountain

display_name: mountain

raclets

display_name: raclets

radar

display_name: radar

rain

display_name: rain

en: Snow Drift Station - Micro Rain Radar


en: terrestrial-laser-scanning mass-flux antarctica drifting-snow meteorological-data snowmicropen

en: Dataset

en: Links to the dataset

en: README

en: Describes the dataset in detail

en:
This dataset contains the data acquired during the expedition to Princess Elisabeth Antarctica Station in December 2016 and January 2017. The dataset consits of meterorological data, drifting snow mass flux data, SnowMicroPen data and Terrestrial Laser Scanning data.

Please refer to the README for more information about the data.

This dataset is the basis of the following publication:

Sommer, C. G., Wever, N., Fierz, C., and Lehning, M.: Wind-packing of snow in Antarctica, The Cryosphere Discuss., https://doi.org/10.5194/tc-2018-36, in review, 2018.

antarctica

display_name: antarctica

drifting-snow

display_name: drifting-snow

mass-flux

display_name: mass-flux

meteorological-data

display_name: meteorological-data

snowmicropen

display_name: snowmicropen

terrestrial-laser-scanning

display_name: terrestrial-laser-scanning

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: Expedition to Princess Elisabeth Antarctica Station, 2016/2017


modeling-snow-failure-with-dem

title_for_slug: modeling-snow-failure-with-dem

en: snow discrete-element-modelling snow-micro-mechanics snow-avalanche snow-failure

en: The zip file contains the data underlying the figures presented in Bobillier et al. (2019). The figures can be reproduced with the python file, which is also provided.

en: Read-me

en: The read-me file contains a description of all the data provided in the zip file.

en: Python file

en: Python file to reproduce the figures in Bobillier et al. (2019)

mimetype: text/x-python

en: This data set includes the modeling results described in the research article by Bobiller et al. (2020). All the figures in the article can be reproduced with the data provided.

discrete-element-modelling

display_name: discrete-element-modelling

snow-avalanche

display_name: snow-avalanche

snow-failure

display_name: snow-failure

snow-micro-mechanics

display_name: snow-micro-mechanics

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Modeling snow failure with DEM


biogas-aus-hofdunger-in-der-schweiz

title_for_slug: biogas-aus-hofdunger-in-der-schweiz

en: switzerland biogas bioenergy review manure

en: Biogas aus Hofdünger in der Schweiz

en: Ziel dieses Whitepapers ist es, Entscheidungsträgern, Verwaltungen und Stakeholdern die aktuellsten Forschungsergebnisse zur Verfügung zu stellen, um die optimale Nutzung von Bioenergie aus Hofdünger in der Schweizer Energiewende zu fördern. Zu diesem Zweck werden die Ergebnisse des Schweizer Kompetenzzentrums für Bioenergieforschung - SCCER BIOSWEET - zusammengefasst und in einem breiteren Kontext dargestellt. Wenn nichts anderes erwähnt wird, beziehen sich die Ergebnisse auf die Schweiz und im Falle der Ressourcen auf die heimischen Biomassepotenziale.

bioenergy

display_name: bioenergy

biogas

display_name: biogas

manure

display_name: manure

review

display_name: review

switzerland

display_name: switzerland


en: long-term relative-humidity meteo meteorology swiss-forest-lab par climate-change precipitation wind-speed temperature

en: Data plot of the forest station (VOB)

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

en: Site aerial photo (Vordemwald)

en: Site aerial photo from both Vordemwald stations. Source: Google. 2016. Site aerial photo from both Vordemwald stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Forest station data (VOB)

en: Data file.

en: Field station data (VOF)

en: Site photo of the forest station (VOB)

en: Site photo of the forest station from the side.

en: Data plot of the field station (VOF)

en: Site photo of the field station (VOF)

en: Site photo of the field stationfrom the side.

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Vordemwald in Switzerland where one station is located within a natural mixed forest stand (VOB) with European silver fir (_Abies alba_; 110 yrs) and oak trees (_Quercus sp._; 190-210 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, VOF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Vordemwald is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

en: Vordemwald, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1996 onwards


maintainer: Matthias Haeni

en: meteorology meteo temperature long-term relative-humidity wind-speed precipitation climate-change par swiss-forest-lab

en: Data plot of the forest station (NAB)

en: Site photo of the forest station (NAB)

en: Data plot of the field station (NAF)

en: Site photo of the field station (NAF)

en: Site photo of the field station form the side.

en: Site aerial photo (Nationalpark)

en: Site aerial photo from both Nationalpark stations. Source: Google. 2016. Site aerial photo from both Nationalpark stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Field station data (NAF)

en: Forest station data (NAB)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Nationalpark in Switzerland where one station is located within a natural coniferous forest stand (NAB) with mountain pine (_Pinus mugo_; 210 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, NAF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Nationalpark is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Nationalpark, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


maintainer: Yohann Chauvier

en: r-package occurrence-records conservation accepted-name taxonomy synonyms landsca flags species-observations ecoregions hard-limit gbif large-scale species-distribution range-maps filtering

en: gbif.range R package version 0.2 (deprecated)

en: gbif.range R package version 1.1 (current)

en: GitHub URL

en: wsl.gbif R package version 0.1 (deprecated)

en: Test version, not to be used.

en: Although species range may be obtained using expert maps or modeling methods, expert data is often species-limited and statistical models need more technical expertise as well as many species observations. When unavailable, such information may be extracted from the Global Biodiversity Information facility (GBIF), the largest public data repository inventorying georeferenced species observations worldwide. However, retrieving GBIF records at large scale may be tedious if users are unaware of specific tools and functions that need to be employed. Here we present *gbif.range*, an R library that contains automated methods to generate species range maps from scratch using in-house ecoregions shapefiles and an easy-to-use GBIF download wrapper. Finally, this library also offers a set of additional very useful parameters and functions for large GBIF datasets (generate doi, extract GBIF taxonomy, records filtering...). [gbif.range R project](https://github.com/8Ginette8/gbif.range)

accepted-name

display_name: accepted-name

conservation

display_name: conservation

ecoregions

display_name: ecoregions

filtering

display_name: filtering

flags

display_name: flags

gbif

display_name: gbif

hard-limit

display_name: hard-limit

landsca

display_name: landsca

large-scale

display_name: large-scale

occurrence-records

display_name: occurrence-records

r-package

display_name: r-package

range-maps

display_name: range-maps

species-distribution

display_name: species-distribution

species-observations

display_name: species-observations

synonyms

display_name: synonyms

taxonomy

display_name: taxonomy

en: gbif.range - An R package to generate species range maps based on ecoregions and a user-friendly GBIF wrapper


en: soil-biodiversity decomposition bacteria detritus wolf biogeochemical-cycling fungi carrion yellowstone carcass

en:
Site	ID for different carcasses across the Yellowstone Landscape
Location	Sampling location, either under carcass or control plot
Species	Indicating whether carcass was from bison or elk
Gender	Gener of carcass; F = female, M = male, ? = unknown
Lat	Latitude
Long	Longitude
DSK	Days since kill; in days
Soil.N	Soil N concentration (%)
Soil.C	Soil C concentration (%)
Veg.C	Vegetation C concentration (%); dominant grasses sampled
Veg.N	Vegetation N concentration (%); dominant grasses sampled
Veg.CtoN	Vegetation C to N ratio; dominant grasses sampled
Soil.resp	Soil respiration (g m-2 h-1)
soil.temp	Soil temperature (°C)
Soil.moist	Soil moisture (volumentric; %)
CRR	Cotton rotting rate
bacteria.richness	Bacteria richness
bacteria.diversity	Bacteria diverstiy
fungi.richness	Fungi richness
fungi.diversity	Fungi diversity
bacteria.abundance	Bacteria abundance
fungi.abundance	Fungi abundance

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:

Risch, A.C., Frossard, A., Schütz, M., Frey, B., Morris, A.W., Bump, J.K. (accepted) Effects of elk and bison carcasses on soil microbial communities and ecosystem functions in Yellowstone, USA. (accepted). Functional Ecology
doi: ...

Methods
Study area and study sites
This study was conducted in YNP’s Northern Range (NR), located in north-western Wyoming and south-western Montana, USA (~44.9163° N, 110.4169° W). The NR expands over ~1000 km2 and features long cold winters and short dry summers. Grasslands and shrublands dominate the NR that is the home of large migratory herds of bison (winter counts 2017: ~3919 individuals; Geremia, Wallen, & White, 2017) and elk (~5349 individuals) as well as their main predators, approximately five packs of wolves with a total of 33 individuals (Smith et al., 2017). As part of a long-term research program within YNP, wolf predation has been studied since their reintroduction in 1995.
For our study, we received ground-truthed coordinates of bison and elk carcasses from winter 2016/17 (November 2016 through April 2017) from the YNP Wolf Project. Between June 20 and July 1, 2017, we visited 24 carcasses in total. At five sites, we could not sample as the carcasses were no longer found. In total we located remains (hairmats, rumen content, bones, teeth) of 19 adult male and female carcasses (7 bison, 12 elk; Supplementary Table 1). Live body weights of adult bison and elk are approximately 730 kg (male bison), 450 kg (female bison), 330 kg (male elk), and 235 kg (female elk, Meagher, 1973; Quimby & Johnson, 1951).
The kills and subsequent consumption happened between 34 and 173 days prior to our sampling (hereafter “days since kill”, DSK), for which we accounted in our statistics. Note that wolves and other scavengers consumed the soft tissue of the carcasses quickly, hence, there is close to no soft tissue left for decomposition as compared to an intact body left on the soil surface. The 19 carcass sites covered the extent of YNP’s NR, with both bison and elk carcasses showing similar distributions; elevation ranged from 1703 to 2884 m a.s.l. (Supplementary Fig 1 & Supplementary Table 1). The carcasses were all located in grassland or sage-brush shrubland, with or without sparsely scattered trees, and both bison and elk carcasses showed the same distribution of DSK. At each study site, we selected a reference plot (hereafter “control”) that was of comparable size, slope aspect and vegetation to the carcass location (hereafter “carcass”). The control was at least 10 m away (Danell, Berteaux, & Brathen, 2002; Melis et al., 2007) from the carcass itself to ensure the absence of potential direct and indirect carcass effects (paired design; (Bump, Webster, et al., 2009; Bump, Peterson, et al., 2009).

Ecosystem functions and soil properties
We randomly collected 50 g of mineral soil from three locations on both control and carcass plots to a depth of 5 cm with sterile techniques and gently mixed the material to obtain a composite sample. Half the soil sample was immediately bagged in plastic bags (whirl packs), stored in a cooler with ice packs (~5 ºC), sieved (2-mm) and frozen within 4-6 hours of collection to assess soil microbial communities. For this purpose, we extracted total genomic DNA from 0.5 g soil using the PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany). DNA concentrations were measured using PicoGreen (Molecular Probes, Eugene, OR, USA). PCR amplifications of partial bacterial small-subunit ribosomal RNA genes (region V3–V4 of 16S rRNA) and fungal ribosomal internal transcribed spacers (region ITS2) were performed as described previously (Frey et al., 2016). Each sample consisting of 40 ng DNA was amplified in triplicate and pooled before purification with Agencourt AMPure XP beads (Beckman Colter, Berea, CA, USA) and quantified with the Qubit 2.0 fluorometric system (Life Technologies, Paisley, UK). Amplicons were sent to the Genome Quebec Innovation Center (Montreal, Canada) for barcoding using the Fluidigm Access Array technology and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA).
Quality control of bacterial and fungal reads was performed using a customized pipeline (Supplementary Table 2; Frey et al., 2016). Paired-ends reads were matched with USEARCH (Edgar & Flyvbjerg, 2015), substitution errors were corrected using Bayeshammer (Nikolenko, Korobeynikov, & Alekseyev, 2013) and PCR primers were trimmed (allowing for 1 mismatch, read length >300 bp for 16S and >200 bp for ITS primers) using Cutadapt (M. Martin, 2011). Sequences were dereplicated and singleton reads removed prior to clustering into operational taxonomic units (OTUs) at 97% identity using USEARCH (Edgar, 2013). The remaining centroid sequences were tested for the presence of ribosomal signatures using Metaxa2 (Bengtsson-Palme et al., 2015) or ITSx (Bengtsson-Palme et al., 2013). Taxonomic assignments of the OTUs were obtained using Bayesian classifier (Wang, Garrity, Tiedje, & Cole, 2007) with a minimum bootstrap support of 60% implemented in mothur (Schloss et al., 2009) by querying the bacterial and fungal reads against the SILVA Release 128 (Quast et al., 2013) and UNITE 8.0 (Abarenkov et al., 2010) reference databases for 16S and ITS OTUs, respectively.
Abundances of the bacterial 16S rRNA gene and fungal ITS amplicon were determined by quantitative real-time PCR (qPCR) on an ABI7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) as described previously (Frossard et al., 2018). The same primers (without barcodes) and cycling conditions as for the sequencing approach were used for the 16S and ITS qPCR. Three standard curves per target region were obtained using tenfold serial dilutions of plasmids generated from cloned targets (Frey, Niklaus, Kremer, Lüscher, & Zimmermann, 2011). Data were converted to represent mean copy number of targets per gram of soil (dry weight).
The other half of the soil sample was bagged in paper, dried to constant weight at 60°C, passed through a 2 mm sieve and analyzed for total C and N concentration with a CE Instruments NC 2100 soil analyzer (CE Elantech Inc., Lakewood NJ, USA). We also collected 20 mature and undamaged leaves of the dominant grass species growing on control and carcass sites, but taxa were not recorded. The plant material was dried at 60°C, finely ground till homogenized and also analyzed to obtain total C and N concentrations. Soil temperature (10 cm depth) was measured with a waterproof digital thermometer (Barnstead International, Dubuque IA, USA) at three locations each at the control and carcass site. Soil moisture (0 – 10 cm depth) was measured with time domain reflectometry (Field-Scout TDR-100; Spectrum Technologies, Plainfield IL, USA) at five randomly chosen points on control and carcass sites. We measured soil respiration at five randomly chosen points at both control and carcass sites with a PP-Systems SRC-1 soil respiration chamber (closed circuit) attached to a PP-Systems EGM-4 infrared gas analyzer (PP-Systems, Amesbury, MA, USA). For each measurement the soil chamber (15 cm high; 10 cm diameter) was tightly placed on the soil surface, after clipping plants to avoid measuring plant respiration or photosynthesis. Measurements were conducted over 120 s.
In addition, we assessed the decomposition rates of standardized OM using the cotton strip assay (Latter & Howson, 1977; Latter & Walton, 1988). Cotton cloth tensile strength loss (CTSL) is a measure of decomposition, and an index to express the combined effect of soil microclimatic, physical, chemical and biological properties on decomposition while accounting for OM quality (Latter & Walton, 1988; Risch, Jurgensen, & Frank, 2007; Withington & Sanford Jr., 2007). We placed five 20 cm wide x 13 cm long sheets of 100% unbleached cotton cloth (American Type SM 1/18’’, Warp: 34/1, Weft: 20/1, Weave plain, 29.5 picks/cm warp, 22 picks/cm weft, 237 g/m2; Daniel Jenny & Co., Switzerland;) at each carcass and control site vertically into the soil by making slits with a flat spade to a depth of 12 cm. We inserted each cloth with the spade, and then pushed the slit closed to assure tight contact with the soil. The cloths were retrieved after 18 to 27 days. After retrieval, the cloths were air-dried, remaining soil gently removed by hand, and 1.5 cm wide strips were cut at the 3.5-5.0 cm (top) and the 9-10.5 cm (bottom) soil depth. The strips were equilibrated at 50 % relative humidity and 20°C for 48 hours (climate chamber) prior to strength testing (Scanpro Awetron TH-1 tensile strength tester; AB Lorentzen and Wettre, Kista, Sweden). Cotton rotting rate (CRR) = (CTScontrol - CTSfinal/CTSfinal)1/3 * (365/t), where CTScontrol is the cotton tensile strength of a control cloth and CTSfinal the cotton tensile strength of the incubated sample, t is the incubation period in days. Control cloths were inserted into the ground and immediately retrieved to account for tensile strength loss associated with cloth insertion. We averaged the CRR of top and bottom strips for further analyses as no difference was found between the two. All sampling and cloth insertion took place between June 20 and July 1, 2017, cloths were retrieved between July 17 and 20, 2017. Soil respiration, average CRR, vegetation N concentration and vegetation C:N ratio are defined as ecosystem functions, soil C and N concentration, soil temperature and moisture as soil abiotic properties, and bacterial and fungal richness (number of taxa), diversity (Shannon) and abundance as soil biotic properties.

Statistical analyses
Univariate analyses for ecosystem functions, soil biotic and abiotic properties
We tested whether individual ecosystem functions, soil biotic and abiotic properties differed between carcass and control (“Location”), bison and elk (“Species”) and days since kill (“DSK”). For this purpose, we used linear mixed effect models (LMM, “nlme” package v 3.1 – 131.1 in R v 3.4.4; Pinheiro, Bates, DebRoy, & Sarkar, 2018; R Core Team, 2019) with Location, Species, Location x Species and DSK as fixed effects. Site was included as random effect to account for the paired design. We developed a separate model for all dependent variables. All but bacterial richness, fungal richness, fungal diversity and vegetation N concentration were natural-log transformed to meet model assumptions. For each LMM, we calculated contrasts to assess the specific comparisons we were interested in with the “lsmeans” package v 2.27-62 (Lenth & Love, 2018): 1) carcass vs control, 2) carcass bison vs control bison, and 3) carcass elk vs control elk. We also tested whether we had differences between bison and elk carcasses or the sites where bison and elk were killed and included contrasts 4) carcass bison vs carcass elk and 5) control bison vs control elk.
We calculated the log response ratio (LRR = ln[carcass/control]) to obtain carcass effects for all variables for both species separately. LRR < 0 indicates higher value at control compared to carcass, LRR > 0 indicates higher values at carcass compared control. We used LRRs for visualization and to assess spatial patterns in carcass effects across YNP. For this purpose we calculated the Moran’s I statistic for each ecosystem function, soil biotic and abiotic property based on a latitude-longitude matrix with the “moran.test” function in the “spdep” package version 1.1-3 (Bivand et al., 2019).
Multivariate analyses
Rare OTUs, defined as OTUs with a low abundance of reads, were retained in multivariate methods because they only marginally influence these analyses (Gobet, Quince, & Ramette, 2010). Bray–Curtis dissimilarity matrices were generated based on square-root-transformed matrices. We used Principal Coordinate Analyses (PCoA) to assess how soil bacterial and fungal communities differed between control and carcass of bison and elk (“vegan” package v 2.5-4, Oksanen et al., 2019). We then extracted PCoA axes scores 1 and 2 and used LMM (“nlme” package) with Location, Species, Location x Species and DSK as fixed effects. Site was, again, included as random effect. We again calculated the contrasts as described above using the “lsmeans” package. We also assessed how ecosystem functions, and soil abiotic and biotic properties were related to the soil bacteria and fungi community structure associated with bison and elk control and carcasses using the “envfit” function in the “vegan” package (Oksanen et al., 2019).
Indicator species analyses were performed using the multipatt function implemented in the “indicspecies” package version 1.7.6 with 100000 permutations (De Caceres & Jansen, 2016). This step allowed to identify OTUs that led to changes in multivariate patterns between control and carcass of both bison and elk separately (De Cáceres, Legendre, & Moretti, 2010). The multipatt function uses a point biserial correlation coefficient statistical test. Indicator OTUs were defined as bacterial and fungal OTUs with more than 50 sequences, i.e., removing rare taxa and taxa with low abundances containing little indicator information (Rime et al., 2015) and that were significantly correlated with Location (p < 0.05, correlation coefficient > 0.3). A heatmap of these OTUs were generated with the vegan and ggplot2 packages. The indicator analyses were performed in R version 3.3.3 (R Core Team, 2017).

References
Abarenkov, K., Henrik Nilsson, R., Larsson, K.-H., Alexander, I. J., Eberhardt, U., Erland, S., … Kõljalg, U. (2010). The UNITE database for molecular identification of fungi – recent updates and future perspectives. New Phytologist, 186(2), 281–285. doi:10.1111/j.1469-8137.2009.03160.x
Bengtsson-Palme, J., Hartmann, M., Eriksson, K. M., Pal, C., Thorell, K., Larsson, D. G. J., & Nilsson, R. H. (2015). metaxa2: improved identification and taxonomic classification of small and large subunit rRNA in metagenomic data. Molecular Ecology Resources, 15(6), 1403–1414. doi:10.1111/1755-0998.12399
Bengtsson-Palme, J., Ryberg, M., Hartmann, M., Branco, S., Wang, Z., Godhe, A., … Nilsson, R. H. (2013). Improved software detection and extraction of ITS1 and ITS2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data. Methods in Ecology and Evolution, 4(10), 914–919. doi:10.1111/2041-210X.12073
Bivand, R., Altman, M., Anselin, L., Assuncao, R., Berke, O., Blanchet, G., … Yu, D. (2019). spdep: Spatial dependence, weighthing schemes, statistics. R package version 1.1-3.
Bump, J. K., Peterson, R. O., & Vucetich, J. A. (2009). Wolves modulate soil nutrient heterogeneity and foliar nitrogen by configuring the distribution of ungulate carcasses. Ecology, 90(11), 3159–3167.
Bump, J. K., Webster, C. R., Vucetich, J. A., Peterson, R. O., Shields, J. M., & Powers, M. D. (2009). Ungulate carcasses perforate ecological filters and create biogeochemical hotspots in forest herbaceous layers allowing trees a competitive advantage. Ecosystems, 12(6), 996–1007. doi:10.1007/s10021-009-9274-0
Danell, K., Berteaux, D., & Brathen, K. A. (2002). Effect of muskox carcasses on nitrogen concentration in tundra vegetation. Arctic, 55(4), 389392.
De Caceres, M., & Jansen, F. (2016). indicspecies: relationship between species and groups of species. R package version 1.7.6.
De Cáceres, M., Legendre, P., & Moretti, M. (2010). Improving indicator species analysis by combining groups of sites. Oikos, 119(10), 1674–1684. doi:10.1111/j.1600-0706.2010.18334.x
Edgar, R. C. (2013). UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods, 10, 996.
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Please cite this paper together with the citation for the datafile.

bacteria

display_name: bacteria

biogeochemical-cycling

display_name: biogeochemical-cycling

carcass

display_name: carcass

carrion

display_name: carrion

decomposition

display_name: decomposition

detritus

display_name: detritus

fungi

display_name: fungi

soil-biodiversity

display_name: soil-biodiversity

wolf

display_name: wolf

yellowstone

display_name: yellowstone

en: Elk and bison carcasses in Yellowstone, USA