en: landslide-early-warning soil-moisture soil-water-matrix-potential soil-temperature soil-water-content

en: In-situ soil moisture measurements, Trub, 2021-2023

en:
Volumetric soil water content (m3 m-3), soil water potential (mbar) and soil temperatures (oC) measured in two profiles and four depths each, as well as air temperature (oC) and precipitation (mm). Time intervall: 1 hour. Measurement period: April 2021-Dec 2023.
Location: slope, meadow
For a detailed description of site, soil and vegetation: see attached document.

en: In-situ soil moisture measurements, Langnau i E, 2021-2023

en:
Volumetric soil water content (m3 m-3), soil water potential (mbar) and soil temperatures (oC) measured in two profiles and four depths each. Meteorological variables, such as air temperature or precipitation can be received from MeteoSwiss.
Time intervall: 1 hour. Measurement period: June 2021-Dec 2023.
Location: flat terrain, meadow (measurement site of MeteoSwiss)
For a detailed description of site, soil and vegetation: see attached document.

en: In-situ soil moisture measurements, Napf, 2021-2023

en:
Volumetric soil water content (m3 m-3), soil water potential (mbar) and soil temperatures (oC) measured in two profiles and four depths each. Meteorological variables such as air temperature or precipitation can be received from MeteoSwiss.
Time intervall: 1 hour.
Measurement period: June 2021-Dec 2023.
Location: flat terrain, meadow (official MeteoSwiss station)
For a detailed description of site, soil and vegetation: see attached document.

en: In-situ soil moisture measurements, Ranflüh, 2021-2023

en:
Volumetric soil water content (m3 m-3), soil water potential (mbar) and soil temperatures (oC) measured in two profiles and four depths each, as well as air temperature (oC) and precipitation (mm). Time intervall: 1 hour. Measurement period: April 2021-Dec 2023.
Location: hillslope, meadow
For a detailed description of site, soil and vegetation: see attached document.

en: In-situ soil moisture measurements, Wasen i.E., flat location, 2019-2023

en:
Volumetric soil water content (m3 m-3), soil water potential (mbar) and soil temperatures (oC) measured in two profiles and four depths each, and two additional soil temperature measurements (oC), air temperature (oC) and precipitation (mm). Time intervall: 1 hour. Measurement period: April 2019-Dec 2023.
Location: flat terrain, meadow
For a detailed description of site, soil and vegetation: see attached document.

en: Detailed description of soil moisture measurment sites, soil properties and sensor installations (in German)

en: In-situ soil moisture measurements, Wasen i.E., steep slope, 2019-2023

en:
Volumetric soil water content (m3 m-3), soil water potential (mbar) and soil temperatures (oC) measured in two profiles and four depths each, and two additional soil temperature measurements (oC), air temperature (oC) and precipitation (mm). Time intervall: 1 hour. Measurement period: April 2019-Dec 2023.
Location: steep hillslope, meadow
For a detailed description of site, soil and vegetation: see attached document.

en: In the context of landslide early warning research, soil moisture (e.g. volumetric soil water content and soil water potential) has been measured at six locations in the Napf-region (Emmental, central Switzerland) since April 2019. Here, we provide the hourly mean values of all sensors from all 6 locations after a comprehensive data control, filtering and aggregation (from 10-min to 1-hour values). A sub-set of these data (for the locations Wasen i.E.) has previously been published in envidat.ch at https://www.doi.org/10.16904/envidat.369 applying the same data post-processing method. The dataset is complemented with meteorological data (locally measured air temperature and precipitation) as well as soil temperature. A detailed description of the sites and measurement installations can be found in a separate report (attached with the data).

landslide-early-warning

display_name: landslide-early-warning

soil-moisture

display_name: soil-moisture

soil-temperature

display_name: soil-temperature

soil-water-content

display_name: soil-water-content

soil-water-matrix-potential

display_name: soil-water-matrix-potential

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: In-situ soil moisture measurements Napf-region


maintainer: Stephan Zimmermann

en: biogeochemical-cycling calcium fagus-orientalis-lipsky magnesium potassium

state: active

type: dataset

en: Concentrations of Ca, Mg, K, and Na in throughfall, litterflow and soil solution of a Hyrcanian forst in Northern Iran

format: XLS

en:
Throughfall, litterflow and soil solution were sampled during one whole year under five Oriental beech trees in a mixed Hyrcanian beech forest. The amounts of Ca2+, Mg2+, K+ and Na+ in these fluxes were calculated based on their concentrations and the sampled volumes, and subsequently compared with the respective fluxes in the rainfall and soil solution of an adjacent forest gap.
In addition six soil profiles, one close to every single tree and one in the forest gap, were analyzed for pH, CaCO3, organic matter and texture.

biogeochemical-cycling

display_name: biogeochemical-cycling

calcium

display_name: calcium

fagus-orientalis-lipsky

display_name: fagus-orientalis-lipsky

magnesium

display_name: magnesium

potassium

display_name: potassium

educ

fr: Éducation, culture et sport

de: Bildung, Kultur und Sport

en: Education, culture and sport

it: Istruzione, cultura e sport

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: Base cation dynamics in an Oriental beech forest


maintainer: Anita Risch

en: vertebrates grassland exclusion ecosystem-functions invertebrates ecosystem-coupling grazing swiss-national-park ecosystem-multifunctionality subalpine

en: Datafile

en:
ID: Plot ID composited of Fence & Treatment number (90 plots total)

Grassland: Name of the six subalpine grasslands where the exclosure setups were erected. In large grasslands (GRIM, MING, STAB), we established four hierarchical exclosure setups (two in short- and two in tall-grass vegetation), whereas in the small grasslands (BOTS, DADA, MARG) only one setup was erected per vegetation type. BOTS = Val dal Botsch, GRIM = Grimmels, MING = Val Mingér, STAB = Alp Stabelchod, DADA = Stabelchod da daint, MARG = Margunet

Fence: Number of exclosure setup (1 - 18). Note that uneven numbers (1,3,5,7,9,11,13,15,17) represent setups established on short-grass vegetation, even numbers (2,4,6,8,10,12,14,16,18) setups established in tall-grass vegetation
Vegetation	Vegetation type, 1 = short-grass vegetation, 2 = tall-grass vegetation; (9 replicate fences on each vegetation type)
Treatment	Progressive animal exclusion, numbers indicate which animals had access to the plot: 1 = Large/medium/small mammals, and invertebrates have access ("L/M/S/I"), 2 = Medium/small mammals, and invertebrates have access (“M/S/I”), 3 = Small mammals and invertebrates have access (“S/I”), 4 = Invertebrates have access (“I”), 5 = No animals have access (“None”)

Bulkden:	Fine fraction soil bulk density (g cm-3)

Soil.moist	: Soil moisture (%)

Soil.org.C: Rhizosphere soil C content (%)

pH: Soil pH

Nema.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil nematode community composition

Nema.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil nematode community composition

Micro.PC1: 	First principal component (PC1) of principal component analysis (PCA) of the soil microbial community composition

Micro.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil microbial community composition

Abv.Inv.PC1: First principal component (PC1) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Abv.Inv.PC2: Second principal component (PC2) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Arthr.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil arthropod community composition

Arthr.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil arthropod community composition

Plant.PC1: First principal component (PC1) of principal component analysis (PCA) of the plant community composition

Plant.PC2: Second principal component (PC2) of principal component analysis (PCA) of the plant community composition

Root.biomass: Root biomass (g m-2)

Micro.biomass	: Microbial biomass carbon (mg kg-1)

Plant.richness: Plant species richness

Plant.N: Plant tissue nitrogen content (%)

Soil.N.min: Soil net nitrogen mineralisation (kg ha-1 season)

Soil.resp: Soil carbon dioxide emissions (soil respiration; g m-2 h-1)

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:
 > Risch AC, Ochoa-Hueso R, van der Putten WH, Bump JK, Busse MD, Frey B, Gwiazdowicz DJ, Page-Dumroese DS, Vandegehuchte ML, Zimmermann S, Schütz M. Size-dependent loss of aboveground animals differentially affects grassland ecosystem coupling and functions. 2018. Nature Communications 9: 3684. [doi: 10.1038/s41467-018-06105-4](https://doi.org/10.1038/s41467-018-06105-4).

Please cite this paper together with the citation for the datafile.

#Methods
Study sites
The experimental exclosure setups were installed within the SNP (IUCN category Ia preserve; Dudley 2008), in south-eastern Switzerland. The park covers 172 km2 of forests and subalpine and alpine grasslands along with scattered rock outcrops and scree slopes. The entire area has been protected from human impact (no hunting, fishing, camping or off-trail hiking) since 1914. Large, fairly homogenous patches of short- and tall-grass vegetation, which originate from different historical management and grazing regimes, cover the park’s subalpine grasslands entirely. Short-grass vegetation developed in areas where cattle used to rest (nutrient input) prior to the park’s foundation (14th century to 1914) (Schütz and others 2003, 2006) and is dominated by lawn grass species such as Festuca rubra L., Briza media L. and Agrostis capillaris L. (Schütz and others 2003, 2006). Today, this vegetation type is intensively grazed by diverse vertebrate and invertebrate communities that inhabit the park and consume up to 60% of the available biomass (Risch and others 2013). Tall-grass vegetation developed where cattle formerly grazed, but did not rest, and is dominated by rather nutrient-poor tussocks of Carex sempervirens Vill. and Nardus stricta L. (Schütz and others 2003, 2006). This vegetation type receives considerably less grazing, with only roughly 20% of the biomass consumed (Risch and others 2013). Consequently, the two vegetation types together represent a long-term trajectory of changes in grazing regimes. Underlying bedrock of all grasslands is dolomite, which renders these grasslands rather poor in nutrients regardless of former and current land-use regimes.
Experimental design
To progressively exclude aboveground vertebrate and invertebrate animals, we established 18 size-selective exclosure setups (nine in short-grass, nine in tall-grass vegetation) distributed over six subalpine grasslands across the SNP (Risch and others 2013, 2015). Elevation differences of exclosure locations did not exceed 350 m (between 1975 and 2300 m a.s.l.). The exclosures were established immediately after snowmelt in spring 2009 and were left in place for five consecutive growing seasons (until end of 2013). They were, however, temporarily dismantled every fall (late October after first snowfall) to protect them from avalanches. They were re-established in the same location every spring immediately after snowmelt. Each size-selective exclosure setup consisted of five plots (2 x 3 m) that progressively excluded aboveground vertebrates and invertebrates from large to small. The plots are labelled according to the guilds that had access to them “L/M/S/I”, “M/S/I”, “S/I”, “I”, “None”; L = large mammals, M = medium mammals, S = small mammals, I = invertebrates, None = no animals had access. As we only had permission to have the experimental setup in place for five consecutive growing seasons, the experiment had to be completely dismantled in the late fall of 2013 and all material removed from the SNP.
Our exclosure design was aimed at excluding mammalian herbivores, but naturally also excluded the few medium and small mammalian predators, as well as the entire aboveground invertebrate food web. A total of 26 large to small mammal species can be found in the SNP, but large apex predators are missing (wolf, bear, lynx) . Reptiles, amphibians and birds are scarce to absent in the subalpine grasslands under study. Only two reptile species occur in the park and they are confined to rocky areas that warm up enough for them to survive. One frog species spawns in an isolated pond far from our grasslands. Only three bird species occasionally feed on the subalpine grasslands. Using game cameras (Moultrie 6MP Game Spy I-60 Infrared Digital Game Camera, Moultrie Feeders, Alabaster, AL, USA), we did observe that the medium- and small-sized mammals (marmot/hares and mice) were not afraid to enter the fences and feed on their designated plots. We never spotted reptiles, amphibians or birds on camera. We distinguished between 59 higher aboveground-dwelling invertebrate taxa that our size-selective exclosures excluded (see also methods for aboveground-dwelling invertebrates below).
The “L/M/S/I” plot (not fenced) was located at least 5 m from the 2.1 m tall and 7 x 9 m large main electrical fence that enclosed the other four plots. The bottom wire of this fence was mounted at 0.5 m height and was not electrified to enable safe access for medium and small mammals, while fencing out the large ones. Within each main fence, we randomly established four 2 x 3 m plots separated by 1-m wide walkways from one another and from the main fence line: 1) the “M/S/I” plots were unfenced, allowing access to all but the large mammals; 2) the “S/I” plots (10 x 10 cm electrical mesh fence) excluded all medium-sized mammals. Note that the bottom 10 cm of this fence remained non-electrified to enable safe access for small mammals; 3) the “I” plots (2 x 2 cm metal mesh fence) excluded all mammals. We double-folded the mesh at the bottom 50 cm to reduce the mesh size to smaller than 1 x 1 cm openings; and 4) the “None” plots were surrounded by a 1 m tall mosquito net (1.5 x 2 mm) to exclude all animals. The top of the plot was covered with a mosquito-meshed wooden frame mounted to the corner posts (roof). We treated these plots a few times with biocompatible insecticide (Clean kill original, Eco Belle GmbH, Waldshut-Tiengen, Germany) to remove insects that might have entered during data collection or that hatched from the soil, but amounts were negligible and did not impact soil moisture conditions within these plots.
To assess whether the design of the “None” exclosure (mesh and roof) affected the response variables within the plots and, therefore, influenced the results, we established an additional six “micro-climate control” exclosures (one in each of the six grasslands) (Risch and others 2013, 2015). These exclosures were built as the “None” exclosures but were open at the bottom (20 cm) of the 3-m side of the fence facing away from the prevailing wind direction to allow invertebrates to enter. A 20-cm high and 3-m long strip of metal mesh was used to block access to small mammals. Thus, this construction allowed a comparable micro-climate to the “None” plots, but also a comparable feeding pressure by invertebrates to the “I” plots. We compared various properties within these exclosures against one another to assess if our construction altered the conditions in the “None” plots. We showed that differences in plant (e.g., vegetation height, aboveground biomass) and soil properties (e.g., soil temperature, moisture) found between the “I” and the “None” treatments were not due to the construction of the “None” exclosure, but a function of animal exclusions, although the amount of UV light reaching the plant canopy was significantly reduced (Risch and others 2013).
Aboveground invertebrate sampling
Aboveground invertebrates were sampled with two different methods to capture both ground- and plant-dwelling organisms: 1) we randomly placed two pitfall traps (67 mm in diameter, covered with a roof) filled with 20% propylene glycol in one 1 x 1 m subplot of the 2 x 3 m treatment plots in spring 2013 (May) and emptied them every two weeks until late September 2013 (Vandegehuchte and others 2017b, 2018). A pitfall trap consisted of a plastic cylinder (13 cm depth, 6.75 cm diameter). Within each cylinder we placed a 100 ml plastic vial with outer diameter 6.70 cm and on top of the cylinder we placed a plastic funnel to guide the invertebrates into the vials. Each trap was cover with a cone-shaped and transparent plastic roof to protect the trap from rain (Vandegehuchte and others 2017b, 2018). Note that in the “None” plots only one trap was placed as control to check for effectiveness of the exclosure. 2) We vacuumed all invertebrates from a 60 x 60 cm area on another 1 x 1 m subplot with a suction sampler (Vortis, Burkhard manufacturing CO, Ltd., Rickmansworth, Hertfordshire, UK) every month from June to September 2013 (Vandegehuchte and others 2017b, 2018). For this purpose, we quickly placed a square plastic frame (60 x 60 x 40 cm) with a closable mosquito mesh sleeve attached to the top edge into the plot from the outside. The suction sample was then inserted into through the sleeve and operated for 45 s to collect the invertebrates (Vandegehuchte and others 2017b, 2018).
We sorted the ≈100 000 individuals collected with both methods by hand and identified each individual morphologically to the lowest taxonomic level feasible (59 taxa, including orders, suborders, subfamilies, families; phylum for Mollusca). These taxa belonged to the following feeding types: 19 herbivores, 16 detritivores, 9 predators, 8 mixed feeders, 5 omnivores and 2 non-classified feeders (or not feeding as adults) (Vandegehuchte and others 2017b). We summed the numbers from the two pitfall traps and the suction sampling over the course of the 2013 season to represent the aboveground invertebrate abundance and community composition of a plot. Note: we did not specifically attempt to catch flying invertebrates with e.g., sticky traps, thus a few flying insects may have been missed with our vacuum sampling approach.
Sampling of plant properties
The vascular plant species composition was assessed at peak biomass every summer (July) by estimating the frequency of occurrence of each species with the pin count method in each plot (Frank and McNaughton 1990). A total of 172 taxa occurred within our 90 plots and we calculated plant species richness for each plot separately. We used the 2013 data in this study. Plant quality was assessed every year in July and September; here we use plant quality at the end of the experiment (September 2013). Two 10 x 100 cm wide strips of vegetation per plot were clipped, combined, dried at 65°C, and ground (Pulverisette 16, Fritsch, Idar-Oberstein, Germany) to pass through a 0.5 mm sieve. Twenty randomly selected samples across all treatments were analysed for N (Leco TruSpec Analyser, Leco, St. Joseph, Michigan, USA) (Vandegehuchte and others 2015). Nitrogen concentrations of the other samples were then estimated from models established for the experiment and the entire SNP relating Fourier transform-near infrared reflectance (FT-NIR) spectra to the measured values of N using a multi-purpose FT-NIR spectrometer (Bruker Optics, Fällanden, Switzerland) (Vandegehuchte and others 2015). Root biomass was sampled every fall by collecting five 2.2 cm diameter x 10 cm deep soil samples (Giddings Machine Company, Windsor, CO, USA) per plot (450 samples year-1). The samples were dried at 30 °C and roots were sorted from the sample by hand. We sorted each sample for 1 h which allowed to retrieve over 90% of all roots present in the samples (Risch and others 2013). The roots were then dried at 65 °C for 48 and weighed to the nearest mg. We averaged the values per plot and used the 2013 data only in this study.
Sampling of edaphic communities
In 2009, 2010, and 2011 we collected three composited soil samples (5 cm diameter x 10 cm depth; AMS Samplers, American Falls, ID, USA) and assessed bacterial community structure using T-RFLP profiling (Liu and others 1997; Blackwood and others 2003; Hodel and others 2014). We detected a total of 89 operational taxonomic units (OTUs). These values are in accordance with other studies reporting OTU richness (Wirthner and others 2011; Zumsteg and others 2012; Meola and others 2014) using T-RFLP profiling, a method that detects the most abundant, and thus likely, the most relevant, taxa. We averaged the data over the three years of collections for our calculations. Microbial biomass carbon (MBC) was determined with the substrate-induced method (Anderson and Domsch 1978) every fall (September) between 2009 and 2013 by collecting three mineral soil samples (5 cm diameter × 10 cm mineral soil core, AMS Samplers, American Falls, ID, USA). The three samples were combined (90 samples for each sampling year), immediately put on ice, taken to the laboratory, passed through a 2-mm sieve and stored at 4°C. Again, we only used the 2013 data in this study.
Soil samples (5 cm diameter x 10 cm depth) to extract soil arthropods were collected in June, July, and August 2011 with a soil corer lined with a plastic sleeve to ensure an undisturbed sample (total of 270 samples). The plastic line core was immediately sealed on both ends using cling film and put into a cooler. All plots were sampled within three days and the extraction of arthropods started the evening of the sampling day using a high-gradient Tullgren funnel apparatus (Crossley and Blair 1991; Vandegehuchte and others 2015). Samples were kept in the extractor for four days and the soil arthropods were collected in 95% ethanol. All individuals were counted and each individual was identified morphologically to the lowest level feasible [76 taxa, including orders, suborders, subfamilies, families (Protura, Thysanoptera, Aphidina, Psylina, Coleoptera, Brachycera, Nematocera, Auchenorryncha, Heteroptera, Formicidae); sub-phylum for Myriapoda, for Acari and Collembola also including morpho-species). Note that we also included larval stages (nine of the 76 taxa) (Vandegehuchte and others 2015). All data were summed over the season. A detailed species list for mites and collembolans is published (Vandegehuchte and others 2017a) [https://doi.org/10.1371/journal.pone.0118679.s001]. Earthworms are rare in the SNP and therefore were not included. We collected eight random 2.2 cm diameter x 10 cm deep soil cores from each plot in September 2013 to determine the soil nematode community composition. The samples were mixed and the nematodes were extracted from 100 ml of fresh soil using Oostenbrink elutriators (Oostenbrink 1960). All nematodes in a 1 ml of the 10 ml extract were counted, a minimum of 150 individuals sample-1 were identified to genus or family level using (Bongers 1988), the numbers of all nematodes were extrapolated to the entire sample and expressed for a 100 g dry sample. In total we identified 63 genus or family levels (Vandegehuchte and others 2015). The list of all the nematodes found is published (Vandegehuchte and others 2015) [http://www.oikosjournal.org/appendix/oik-03341] or DOI: [doi: 10.1111/oik.03341].
We are aware that sampling soil microbes from 2009 to 2011 and soil arthropods in 2011 was not ideal, but we are positive that this does not bias the results. Most of the parameters measured in our experiment either already showed a treatment response after the first growing season (e.g., plant biomass) or did not respond over the entire time experiment (e.g., microbial biomass C). The microbial community composition (2009 – 2011) was highly influenced by inter-annual differences in temperature and precipitation, but did not differ between treatments or vegetation types (Hodel and others 2014). We therefore felt comfortable using the 2009 through 2011 data for describing the soil microbial community in our experimental treatments. Similarly, we are positive that our soil arthropod data are representative. We did assess soil arthropods in August 2012 and found no differences to the August 2011 data. However, we did not feel comfortable combining the 2011 June, July, August data with only August data for 2012 for our analyses.
Sampling of soil properties
We collected three soil samples (5 cm diameter x 10 cm depth) in each plot in September 2013 after removing the vegetation. First, we collected the top layer of mineral soil rich in organic matter, the surface organic layer or rhizosphere, typically 1 to 3 cm in depth with a soil corer (AMS Samples, American Falls, Idaho, USA). Second, we collected a 10 cm mineral soil core beneath this surface layer. The cores for each layer were composited, dried at 65 °C for 48 h and fine-ground to pass a 0.5 mm screen. We then analysed all samples for total C using a Leco TruSpec Analyser (Leco, St. Joseph, Michigan, USA). Mineral soil pH was measured potentiometrically in 1:2 soil:CaCl2 solution with an equilibration time of 30 min.
Soil net N mineralisation was assessed during the 2013 growing season (Risch and others 2015). For this purpose, we randomly collected a 5 cm diameter x10 cm deep soil sample with a soil corer (AMS Samples, American Falls, Idaho, USA) after clipping the vegetation in June 2013. After weighing and sieving (4 mm mesh) the soil, we extracted a 20 g subsample in 1 mol l-1 KCl for 1.5 h on an end-over-end shaker and thereafter filtered it through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnenmühle FineArt GmbH, Dassel, Germany). From these filtrates NO3- concentrations were measured colorimetrically (Norman and Stucki 1981) and NH4+with flow injection analysis (FIAS 300, Perkin Elmer, Waltham Massachusetts, USA) (Risch and others 2015). We dried the rest of the sample 105 °C to constant mass to determine fine,fraction bulk density. A second soil sample was collected within each plot in June 2013 with a corer lined with a 5 x 13 cm aluminium cylinder. The corer was driven 11.5 cm deep into the soil so that the top 1.5 cm of the cylinder remained empty. Into this space we placed a polyester bag (250 µm) filled an ion-exchanger resin to capture the incoming N. The bag was filled with a 1:1 mixture of acidic and alkaline exchanger resin (ion-exchanger I KA/ion exchanger IIIAA, Merck AG, Darmstadt, Germany). We then removed 1.5 cm soil at the bottom of the cylinder and placed a second resin exchanger bag into this space to capture the N leached from the soil column. To assure that the exchange resin was saturated with H+ and Cl- prior to filling the bags, the mixture was stirred with 1.2 ml l-1 HCl for 1 h and then rinsed with demineralized water until the electrical conductivity of the water reached 5 µm cm-1. The cylinder with the resin bags in place was reinserted into the soil with the top flush to the soil surface and incubated for three months. We recollected the cylinders in September 2013. Each resin bag and 20 g of sieved soil (4 mm mesh) from each cylinder were then separately extracted with KCl and NO3- and NH4+ concentrations were measured. Nitrate and NH4+ concentrations of all samples were then converted to a content basis by multiplying their values with fine fraction bulk density. Net N mineralisation was thereafter calculated as the difference between the N content of the samples collected at the end of the three-month incubation (including the N extracted from the bottom resin bag) and the N content at the beginning of the incubation (Risch and others 2015).
Soil CO2 emissions were measured every two weeks between 0900 and 1700 hrs from early May through late September 2013 with a PP-Systems SRC-1 soil respiration chamber (15 cm high, 10 cm diameter; closed circuit) attached to a PP-Systems EGM-4 infrared gas analyser (PP-Systems, Amesbury, MA, USA) on two locations per plot (Risch and others 2013). The chamber was placed on randomly placed, permanently installed PVC collars (10 cm diameter) driven 5 cm into the soil at the beginning of the study (Risch and others 2013). Freshly germinated plants growing within the collars were removed prior to each measurement to avoid measuring plant respiration or photosynthesis. The two measurements collected per plot and sampling date were averaged.
Soil moisture (with time domain reflectometry; Field-Scout TDR-100, Spectrum Technologies, Plainfield, Illionois, USA) and temperature (with a waterproof digital pocket thermometer; Barnstead International, Dubuque, Iowa, USA) were measured at five random locations per plot every two weeks during the growing seasons during the experiment for the 0 to 10 cm depth (Risch and others 2013, 2015). As soil moisture and soil temperature were highly negatively correlated (Risch and others 2013), we only used soil moisture for this study. We used plot-level averages of all values available to capture soil moisture variability during the five years of the experiment. The results remained unchanged when we only used soil moisture from the 2013 growing season.
Numeral calculations and statistical analyses
Ecosystem coupling. We conducted principal component analyses (PCAs; unscaled) at the complete dataset level using the abundances of each taxonomical entity to describe each of the five different communities used in this study: aboveground-dwelling invertebrates, vascular plants, soil microorganisms, soil arthropods and soil nematodes. We retained the first two components (PCA axis 1 and PCA axis 2) of each analysis as we found them to adequately represent the temporal and spatial variability of our 90 treatment plots in previous studies55,67. Together they explained a total of 71.70% of the variation for aboveground invertebrates, 44.36% for plants, 44.85% for soil microorganisms, 61.85% for soil arthropods and 77.19% for soil nematodes. In addition, we used soil pH and soil organic C content as a proxy for soil chemical properties, soil bulk density as a proxy for soil physical properties and soil moisture (negatively correlated with soil temperature) as a proxy for soil micro-climatic conditions for an overall total of fourteen constituents.
We calculated ecosystem coupling9 for each exclosure treatment within each vegetation type (i.e., 2  5 treatment combinations in total) as an integrated measure of pairwise ecological interactions between ecosystem constituents representing ecological communities and the soil abiotic environment. These ecological interactions are defined by non-parametric Spearman rank correlation analyses between two constituents, excluding interactions involving two abiotic constituents (e.g., soil pH vs. soil moisture) and interactions between the first (PC1) and second (PC2) component of each community type, as these are orthogonal by definition. Interactions between abiotic constituents were excluded from the analyses because the focus of our study was on communities and how they interact with one another and their surrounding environment; therefore, including abiotic-abiotic interactions was not of interest here. Given that the effectiveness of our experimental design resulted in that no community composition data of aboveground-dwelling invertebrates was available for the “None” plots (all animals excluded), only thirteen instead of fourteen constituents were included in the ecosystem coupling calculations for this treatment. The complete absence of aboveground invertebrates represents the most extreme case of disturbance between aboveground animal communities and the rest of the ecosystem constituents. This may have resulted in a slight overestimation of ecosystem coupling for these plots.
	Average ecosystem coupling was calculated as follows:
Ecosystem coupling=
where Xi is the absolute Coupling was calculated value of the Spearman’s rho coefficient of the ith correlation for each treatment within each vegetation type (i.e., based on nine replicates each), considering and n is the number of pairwise comparisons considered (n = a total of 80; interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).	Coupling was calculated for each treatment within each vegetation type (i.e., based on nine replicates each), considering a total of 80 interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).
To establish whether constituents were significantly and positively coupled within treatments (i.e., the average of their correlation coefficients were greater than in a null model where correlation only happens by chance), we calculated one-tailed p-values based on permutation tests with 999 permutations.

We considered six ecosystem functions and process rates commonly used to assess ecosystem functioning (Meyer and others 2015; Manning and others 2018). Plant N content represents a measure of forage quality, while plant richness has been shown to stabilise biomass production, thus allowing the system to respond to changes in herbivory. Soil net N mineralisation, soil respiration, root biomass, and microbial biomass represent fluxes or stocks of energy. For all functions and processes higher values represent higher functioning (Manning and others 2018). All these variables were measured in the last year of the experiment (2013). We then quantified ecosystem multifunctionality using the multiple threshold approach (Byrnes and others 2014; Manning and others 2018), which considers the number of functions that are above a certain threshold, over a series of threshold values (typically 10-99%) that are defined based on the maximum value of each function. We weighted all our functions equally for these calculations (Manning and others 2018). The number of functions in a plot with values higher than a given threshold value for the respective function is summed up. The sum represents ecosystem multifunctionality for that plot. Given that choosing any particular threshold as a measure of ecosystem multifunctionality is arbitrary, we calculated the average of thresholds from 10-90% (in 10% intervals) as a more integrated representation of ecosystem multifunctionality.
We used Pearson correlations to explore the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and ecosystem multifunctionality by calculating the slopes of all relationships between ecosystem coupling and multifunctionality for all thresholds between 10 and 99%. We also related ecosystem coupling with the average of multifunctionality at thresholds between 30-80% as explained before and considered this correlation as a robust indication of the type of association between these two variables. In addition, we explored the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and individual ecosystem functions. The effects of exclosures and vegetation type on individual functions and multifunctionality were evaluated using linear mixed effects models ('lme' function of the nlme package), with exclosure and vegetation type as fixed effects and fence as a random factor. All statistical analyses and numerical calculations were done in R version 3.4.0 (R Core Team 2016).

#References

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- Vandegehuchte ML, van der Putten WH, Duyts H, Schütz M, Risch AC. 2017a. Aboveground mammal and invertebrate exclusions cause consistent changes in soil food webs of two subalpine grassland types, but mechanisms are system-speciﬁc. Oikos 126:212–23.
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- Wirthner S, Frey B, Busse MD, Schütz M, Risch AC. 2011. Effects of wild boar (Sus scrofa L.) rooting on the bacterial community structure in mixed-hardwood forest soils in Switzerland. Eur J Soil Biol 47:296–302. http://dx.doi.org/10.1016/j.ejsobi.2011.07.003
- Zumsteg A, Luster J, Göransson H, Smittenberg RH, Brunner I, Bernasconi SM, Zeyer J, Frey B. 2012. Bacterial, Archaeal and Fungal Succession in the Forefield of a Receding Glacier. Microb Ecol 63:552–64. https://doi.org/10.1007/s00248-011-9991-8

ecosystem-coupling

display_name: ecosystem-coupling

ecosystem-functions

display_name: ecosystem-functions

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

exclusion

display_name: exclusion

grassland

display_name: grassland

grazing

display_name: grazing

invertebrates

display_name: invertebrates

subalpine

display_name: subalpine

swiss-national-park

display_name: swiss-national-park

vertebrates

display_name: vertebrates

en: Ecosystem coupling and multifunctionality - exclosure experiment


en: atlantic-region teleo chondrichthyes edna bay-biscay fish-composition actinopterygii dna-metabarcoding

en: Data access

format: SERVICE

en:
Environmental DNA complements scientific trawling in surveys of marine fish biodiversity (Dataset 2020)

In October 2020 we chose 16 sites from the 2020 EVHOE survey for eDNA sampling. The French international EVHOE bottom trawl survey is carried out annually during autumn in the BoB to monitor demersal fish resources. All sites were located on the continental shelf (26–170 m depth), except one on the upper slope with a depth of 1045 m. To perform eDNA sampling, we collected water samples at 15 sites (Figure 1a). At each site, we sampled seawater using Niskin bottles deployed with a circular rosette. There were nine bottles on the rosette, each of them able to hold ∼5 l of water. At each site, we first cleaned the circular rosette and bottles with freshwater, then lowered the rosette (with bottles open) to 5 m above the sea bottom, and finally closed the bottles remotely from the boat. The 45 l of sampled water was transferred to four disposable and sterilized plastic bags of 11.25 l each to perform the filtration on-board in a laboratory dedicated to the processing of eDNA samples. To speed up the filtration process, we used two identical filtration devices, each composed of an Athena® peristaltic pump (Proactive Environmental Products LLC, Bradenton, Florida, USA; nominal flow of 1.0 l min–1 ), a VigiDNA 0.20 μm filtration capsule (SPYGEN, le Bourget du Lac, France), and disposable sterile tubing. Each filtration device filtered the water contained in two plastic bags (22.5 l), which represent two replicates per sampling site. We followed a rigorous protocol to avoid contamination during fieldwork, using disposable gloves and single-use filtration equipment and plastic bags to process each water sample. At the end of each filtration, we emptied the water inside the capsule that we replaced by 80 ml of CL1 conservation buffer and stored the samples at room temperature following the specifications of the manufacturer (SPYGEN, Le Bourget du Lac, France). We processed the eDNA capsules at SPYGEN, following the protocol proposed by Polanco-Fernández et al., (2020). We performed library preparation and sequencing at Fasteris (Geneva, Switzerland). Specifically, we prepared four libraries using the MetaFast protocol (a ligation-based method) and sequenced them separately. We carried out paired-end sequencing using a MiSeq sequencer (2 × 125 bp, Illumina, San Diego, CA, USA) on two MiSeq Flow Cell Kits (v3; Illumina), following the manufacturer’s instructions. We analysed the sequence reads using the OBITools package (http:
//metabarcoding.org/obitools; Boyer et al., 2016), following the protocol described by Valentini et al. (2016).

Data content:
	* rawdata/: contains the raw reads for each individual sample. One archive contains the paired-end reads specified by the _R1 or _R2 suffix as well as individually tagged PCR replicates (if available) together with an archive containing all extraction and PCR blank samples of the library. Reads have been demultiplexed using cutadapt but not trimmed, individual demultiplexing tags and primers remain present in the sequences.
	* taxadata/: contains the table with all detected taxonomy for each sample after bioinformatic processing (see Polanco et al. 2020 for details; https://doi.org/10.1002/edn3.140) and associated field metadata.
	* metadata/: contains two metadata files, one related to the data collected in the field for each filter, and the second related to the sequencing process in the lab (including the tag sequence, library name, and marker information for each sample)

actinopterygii

display_name: actinopterygii

atlantic-region

display_name: atlantic-region

bay-biscay

display_name: bay-biscay

chondrichthyes

display_name: chondrichthyes

dna-metabarcoding

display_name: dna-metabarcoding

edna

display_name: edna

fish-composition

display_name: fish-composition

teleo

display_name: teleo

en: Environmental DNA Marine France Evhoe 2020


en: landscape-ecology european-landscape-convention ecosystem-services landscape landscape-perception

en: This indicator shows the situation of wind energy in Switzerland. Firstly, we locate the current wind turbines and wind farms in Switzerland and compare their location with the wind speed map and the federal government's base map concerning the main wind potential areas. Secondly, we analyse the development of wind energy in Switzerland over the years and the efficiency of such structures.

en: This indicator quantitatively describes land consumption for settlement and the change in Switzerland's settlement area based on data from Switzerland's land use statistics (Swiss Federal Statistical Office FSO).

en: Summer pastures make up the majority of extensively used agricultural land in Switzerland. Summer pastures are located at altitudes that do not allow intensive use of the land, as is the case in the Central Plateau. Fertiliser use is either non-existent or limited. The indicator helps to measure changes in a basic agricultural use that is very important for the mountain landscape. It is a major determinant of landscape character and biodiversity.

media_type: application/zip

en: For many people, "untouched landscape" is the very essence of nature. There are hardly any places left in Switzerland that have no human influence. But you can find regions where human influence is not so obvious. These are areas where no human buildings and installations can be seen. Areas free of facilities are therefore the most natural areas that can be found in Switzerland.

format: ZIP

en: Indicator 4 describes the length and area of roads and railroads for the year 2020, as well as the changes between different points in time. The calculation of length and area consumption is based on the Topographic Landscape Model TLM and TLM3D of the Federal Office of Topography. Before 2014, changes were recorded in a sampling procedure based on landscape elements and extrapolated to subspaces and the whole of Switzerland. Since 2015, however, it has been possible to use data directly for the whole of Switzerland.

en: This indicator measures the average number of different uses of agricultural land in Switzerland. It is thus a measure of the diversity of agricultural land. The agricultural structure survey is used to record the diversity of use.

en: Indicator 31a describes the accessibility of water bodies in Switzerland. Water bodies are popular recreational areas. In order to be used by the population, therefore they should be freely accessible.

en: As a metric for characterising urban sprawl, the measure of urban sprawl (Z or weighted urban permeation WUP) was first propagated by Jaeger et al. (2008). We use this measure here. Urban sprawl is expressed in DSE/m2. The metric was refined within the framework of the National Research Programme 54 "Sustainable settlement and infrastructure development" in the project "Landscape sprawl Switzerland - Quantitative analysis 1935 to 2002 and implications for spatial planning" (Jaeger et al. 2008).

en: This indicator shows the status of transmission lines in Switzerland and analyses the land cover on which these infrastructures are built. Transmission lines are assessed on the basis of TLM data. An extract from TLM_VERSORGUNGSBAUTE (OBJEKTART: Hochspannungsleitungen) is used to calculate the length consumption. In addition, the area statistics layer (NOLC04) is used to differentiate land types into different categories. The total length of transmission lines in Switzerland, in the different biogeographical regions and in the different cantons is calculated by overlay operations for year 2015 and year 2020 (see appendices for further details).

en: Light emissions in 500m resolution for entire Switzerland for the periode 2014-2020 using VIIRS-VCSML

en: This is the general readme for all LABES datasets.

en: Indicator 2 describes the quantitative change in Switzerland's agricultural area excluding summer pastures. It contributes to assessing the basic agricultural use, which is very important for the landscape. This has a significant impact on the character of the landscape and on biodiversity.

en:
This indicator shows the state of vegetation within urban areas in Switzerland. On the one hand, the entire vegetation period is considered, taking into account all data from mid-May to mid-September. On the other hand, an additional analysis focuses on the spring period from mid-May to the end of June in order to observe only the growing season, which in the vast majority of cases is not yet affected by drought stress.
The remote sensing data used to calculate this indicator come from Sentinel-2 MSI and cover the whole of Switzerland. Sentinel-2 is a wide-area, high-resolution, multispectral imaging mission of ESA for monitoring vegetation, soil and water cover. Sentinel-2 Level-1C data were used, which are radiometrically and geometrically corrected top-of-atmosphere products. The corrections include orthorectification and spatial registration on a global reference system (combined UTM projection and WGS84 ellipsoid) with sub-pixel accuracy. Level 1C images are delivered in tiles of 100×100 km. A total of 11 tiles are required to cover the extent of Switzerland.

en: Indicator 11a describes the change in the length of watercourses in Switzerland between different points in time. The changes are systematically recorded on the basis of the changes in the national maps 1:25,000, later Vector25 and from about 2012 using TLM3D. With this evaluation protocol (2022), the sampling procedure of "Landschaft unter Druck" is used for the last time. It consists of 152 sampling rectangles in which the changes are recorded and extrapolated to the whole of Switzerland. From TLM3D (2020) onwards, the extrapolation is guaranteed for the entire area of Switzerland.

en: Indicator 35 describes the accessibility with footpaths and hiking trails less than 2m wide. The basis for this is the Topographic Landscape Model (TLM and TLM3D) of the Federal Office of Topography. The data are given in km/km2 and are broken down by land use. The land use categories settlement, forest and open land are extracted from the area statistics of the Federal Statistical Office. Contrary to the initial calculation in 2015, we have abandoned the official attribute "hiking trail", as it has been shown that official "hiking trails" are also routed over longer distances along major roads. The current definition has to do solely with the width of the path (max. width 2m) and not with the official designation. This is closer to the quality of a trail than the official designation "hiking trail".

en: The degree of imperviousness indicates the sealed surface area as a percentage. The LABES indicator 7 is surveyed with the area statistics Switzerland. The survey interval of the areal statistics is 12 years for the first 3 surveys and 9 years for the fourth survey of the areal statistics; therefore, short-term developments in the change of the sealed surface cannot be recorded.

en: Areas left to nature are areas in which natural processes can take place essentially undisturbed and direct human influences determine the (current) development only to a small extent. In such areas, the composition of animal and plant communities differs from that in the cultivated landscape. The areas left to nature in Switzerland can be divided into "wilderness areas" in the forest (forest wilderness), areas left to nature in the high mountains (alpine fallow) and areas along water bodies (floodplains). The areas may or may not be accessible. Of central importance is the intensity of human activities.

en:
The social indicators for LABES were collected with a Swiss-wide representative survey. The first survey was conducted in 2011 (n = 2814 participants), the second in 2020 (n = 2090 participants). The “Auswertungsprotokolle” inform about the results of each indicator in 2020 and compared to the state of 2011.
Indicator list:
Indicator 23 Ortsbindung / place attachment
Indicator 24 Landschaftliche Schönheit / visual landscape quality or beauty
Indicator 25 Besonderheit (Eigenart und Vergangenheitsbezug)/ place identity
Indicator 27 Wahrgenommener Informationsgehalt der Landschaft: Komplexität, Entdeckungspotenzial, Kohärenz, Lesbarkeit / Kaplans’ preference matrix: complexity, mystery, coherence, legibility

Indicator 29 Faszination / soft fascination
Indicator 30 Authentizität /authenticity
Indicator Neu_16 Erholung und Landschaft (Erholungsindex, Zugang, Outdoorsport) / recreation and landscapes (recreationindex, access, outdoor sports)
Indicator Neu_17 Beobachtete Landschaftscharakterisierung / perceived landscape charachterization
Indicator Neu_18 Beobachtete Landschaftsveränderungen / perceived landscape changes
Indicator Neu_20 Wahrgenommene Landschaftsqualität in den Grundnutzungen oder Texturen (Wald, Landwirtschaft, Siedlungsflächen, Gewässer)/ Perceived landscape quality in the different land use areas (forest, agriculture, settlement areas, water bodies)
Indicator Neu_22 Gesamturteil zur wahrgenommenen Landschaftsqualität / overall satisfaction
Indicator Neu_23 Kulturelle Landschafswerte / cultural values and services of the landscape

en: Indicator 1 quantitatively describes the forest area of Switzerland and its change. The required data on forest area are calculated using the area statistics (Swiss Federal Statistical Office, SFSO).

en: Indicator 8 quantitatively describes the Euclidean distance from each point of a 12.5x12.5m grid outside the building zone to the nearest building. This is a revision based on a new calculation method.

en: This indicator shows the agricultural intensity in Switzerland using satellite data by calculating the NDVI values for each growing season and, in particular, observing the standard deviation of these values over the years. The remote sensing data used to calculate this indicator come from Sentinel-2 MSI and cover the whole of Switzerland. Sentinel-2 is a wide-area, high-resolution, multispectral imaging mission of ESA for monitoring vegetation, soil and water cover. Sentinel-2 Level-1C data were used, which are radiometrically and geometrically corrected top-of-atmosphere products. The corrections include orthorectification and spatial registration on a global reference system (combined UTM projection and WGS84 ellipsoid) with sub-pixel accuracy. Level 1C images are delivered in tiles of 100×100 km. A total of 11 tiles are needed to cover the extent of Switzerland.

en:
Remoteness implies distance from a reference. In the case of an actual journey through a remote landscape, this distance can mean the physical distance from society or from signs of civilisation. In solitude, individuals face the elements of nature and thus move a step away from the backing of security provided by civilised society.
To calculate the remoteness of each grid cell per time period, the "Path Distance" ArcGIS tool is used. This calculates the cost between each grid cell and the settlement areas (pixels with >15 people) as the shortest trajectory. The surface distance is calculated from the horizontal and vertical distance (including the digital elevation model DHM25) and a cost grid, which assigns to each cell the costs incurred when passing through this area. As soon as the trajectory meets (or starts on) a road on which the settlement can be reached, the journey time by public transport or by private means of transport is calculated and the shorter of the two journey times is used.

en: Transport infrastructure such as highways, connecting roads and railroads, as well as settlement areas divide a landscape into individual areas separated from each other. To measure the degree of landscape fragmentation, the metric of "effective mesh size" (meff) was used (Jaeger 2000). It expresses the probability that there are no separating elements, such as traffic routes or settlements, between two randomly selected points. The more separating elements cut up the landscape, the lower this probability and the smaller the effective mesh size.

en:
The Swiss Landscape Monitoring Program (LABES) records both the physical and the perceived quality of the landscape with about 30 indicators. The surveys of the physical aspects are largely based on evaluations of data available throughout Switzerland from swisstopo and the Federal Statistical Office (FSO). Another significant part of the data comes from a nationwide population survey on landscape perception.
This dataset describes data that have been assembled in the 2020 update of the Swiss Landscape Monitoring Program LABES.

ecosystem-services

display_name: ecosystem-services

european-landscape-convention

display_name: european-landscape-convention

landscape

display_name: landscape

landscape-ecology

display_name: landscape-ecology

landscape-perception

display_name: landscape-perception

envidat

en: EnviDat: the Environmental Data Portal

en: LABES 2 Indicators of the Swiss Landscape Monitoring Program


maintainer: Anita C. Risch

en: nitrogen soil nitrification climate herbivore grassland nutrient-network mineralization ammonification global-change fertilization

en: Risch et al. 2020 - Raw data for publication "Global impacts of fertilization and herbivore removal on soil net nitrogen mineralization are modulated by local climate and soil properties"

en:
we assessed how the removal of mammalian herbivores (Fence) and fertilization with growth-limiting nutrients (N, P, K, plus nine essential macro- and micronutrients; NPK) individually, and in combination (NPK+Fence), affected potential and realized soil net Nmin across 22 natural and semi-natural grasslands on five continents. Our sites spanned a comprehensive range of climatic and edaphic conditions found across the grassland biome. We focused on grasslands, because they cover 40-50% of the ice-free land surface and provide vital ecosystem functions and services. They are particularly important for forage production and C sequestration. Worldwide, grasslands store approximately 20-30% of the Earth’s terrestrial C, most of it in the soil (Schimel, 1995; White et al., 2000).

Study sites and experimental design
The 22 sites contributing to this project are part of the Nutrient Network Global Research Cooperative (NutNet, https://nutnet.umn.edu/). Mean annual temperature across our 22 sites ranged from -4 to 22°C, mean annual precipitation from 252 to 1,592 mm, and elevations from 6 to 4,261 m above sea level (Fig. 1, Supplementary Table S1). Soil organic C varied from 0.8 to 7.8%, soil total N from 0.1 to 0.6%, and the soil C:N ratio from 9.1 to 21.5. Soil clay content spanned from 3.0 to 35%, and soil pH from 3.4 to 7.6 (Supplementary Table S2). Thus, the sites covered a wide range of environments in which grasslands occur (Fig. 1, Supplementary Table S1 & S2).
At each site, the effects of nutrient addition and herbivore removal were tested via a randomized-block design (Borer et al., 2014; Supplementary Fig. S1a). Three replicate blocks with 10 treatment plots each were established at each site, with the exception of the site at bldr.us, where only two blocks were established (Supplementary Fig. S1a). The 10 plots were randomly assigned to a nutrient or fencing treatment, but only a subset of four plots was used in the current study, each with a different treatment (see below; Supplementary Fig. S1a). All plots were 5 x 5 m and divided into four 2.5 x 2.5 m subplots (Supplementary Fig. S1b). Each subplot was further divided into four 1 x 1 m square sampling plots, one of which was set aside for soil sampling (Borer et al., 2014; Supplementary Fig. S1b). Plots were separated by at least 1 m wide walkways.
In this study, we collected data from the following four treatments: (i) untreated control plots (Control), (ii) herbivore removal plots (Fence), (iii) plots fertilized with N, P, K, plus nine essential macro and micronutrients (NPK), and (iv) plots with simultaneous fertilizer addition and herbivore removal (NPK+Fence; Supplementary Fig. S1a). The number of years of treatment differed among sites (2 – 9 years since start of treatment; Supplementary Table S1). For the nutrient additions, all sites applied 10 g N m-2 yr-1 as time-release urea; 10 g P m-2 yr-1 as triple-super phosphate; 10 g K m-2 yr-1 as potassium sulfate. A micro-nutrient mix (Fe, S, Mg, Mn, Cu, Zn, B, Mo, Ca) was applied at 100 g m-2 together with K in the first year of treatments but not thereafter.
The vertebrate herbivore removal treatment (Fence) was established by fencing two plots, one control and one NPK plot, within each of the blocks (Supplementary Fig. S1a). We designed the fences so that they would effectively exclude aboveground mammalian herbivores with a body mass of over 50 g (Borer et al., 2014). At the majority of sites, the height of the fences was 180 cm, and the fence design included wire mesh (1 cm holes) on the first 90 cm along with a 30 cm outward-facing flange stapled to the ground to exclude burrowing animals; climbing and subterranean animals may potentially still access these plots (Borer et al., 2014). For slight modifications in fence design at a few sites see Supplementary Table S3. While most sites only had native herbivores, a few sites (4) were also grazed by domestic animals (Supplementary Table S1).
Potential and realized soil net N mineralization, ammonification, nitrification and other soil properties
Each site participating in the study received a package containing identical material from the Swiss Federal Institute for Forest, Snow and Landscape Research (WSL) to be used for sampling and on-site N incubations. For the field incubation, we followed the protocol by Risch et al. (2015, 2019). Briefly, we drove a 5 x 15 cm (diameter x depth) steel cylinder 13.5 cm deep into the soil after clipping the vegetation at randomized locations in each plot. The top 1.5 cm of the cylinder remained empty to capture incoming N from run-off or deposition with a polyester mesh bag (mesh-size 250 µm) filled with 13.2 ± 0.9 g of acidic and alkaline exchanger resin (1:1 mixture; ion-exchanger I KA/ion-exchanger III AA, Merck AG, Darmstadt). The bag was fixed in place with a metal Seeger ring (Bruetsch-Rüegger Holding, Urdorf, Switzerland). We then removed 1.5 cm soil at the bottom of the cylinder and placed another resin bag to capture N leached from the soil column. The exchange resin was saturated with H+ and Cl- prior to filling the bags by stirring the mixture in 1.2M HCl for 1 h and then rinsing it with demineralized water until the electrical conductivity of the water reached 5 μS/cm. The cylinders were then re-inserted into the cored hole, level with the soil surface, and incubated for an average of 42 days (range 40 to 57days). The individual site coordinators chose the timing of incubation to start approximately six weeks prior to peak plant biomass production. All incubations were completed between February 2015 and January 2016 accounting for differences in growing season between northern and southern hemispheres. At the end of the incubation, the cylinders were re-collected and immediately shipped to WSL in an insulated box together with cold packs to halt further mineralization. Gloves were worn at all times to avoid contamination of the samples. Upon arrival at WSL, we extracted the resin bags and a 20 g subsample of sieved soil (4 mm) separately in a 100 ml PE-bottle with 80 ml 1 M KCl for 1.5 h on an end-over-end shaker and filtered through ashless folded filter paper (DF 5895 150, ALBET LabScience). We measured NO3- (colorimetrically; Norman & Stucki, 1981) and NH4+concentrations (flow injection analysis; FIAS 300, Perkin Elmer) on these filtrates.
At the start of the field incubation, we additionally collected two soil cores of 5 x 12 cm (diameter x depth) in each sampling plot and composited them to measure potential soil net Nmin, soil chemical and biological properties (see below). We also collected an additional sample (5 x 12 cm) to assess soil physical properties, which remained within the steel cylinder. Both ends were tightly closed with plastic caps. Cores were carefully packed to avoid further disturbance, and together with the composited soil samples, were shipped to the laboratory at WSL within a few days after collection.
From the composited samples, we extracted an equivalent of 20 g dry soil with KCl, as described above, and measured NO3- and NH4+concentrations. Realized soil net Nmin was then calculated as the difference between the inorganic N content of samples collected at the end of the incubation (plus N extracted from the bottom resin bag) and the N content at the beginning of the incubation; values were scaled to represent daily mineralization rates (mg N kg-1soil d-1; Risch et al., 2015). Realized soil net Nmin values represent an average period of 42 days prior to peak biomass, typically the highest period of biological activity, and not the entire year (Risch et al., 2019).
A second subsample of the composited sample was used to determine potential soil net Nmin in the laboratory (Risch et al., 2019). Briefly, we weighed duplicate samples (8 g dry soil) into 50-ml Falcon tubes. Soil moisture was brought to 60% of the field capacity of each plot, the Falcon tubes tightly closed and then incubated at 20°C for 42 days in a dark room. Every week the Falcon tubes were opened and ventilated. At the end of the incubation, the soil samples were extracted the same way as described above and NO3- and NH4+ was determined. Potential soil net Nmin was calculated as the difference between the N content before and after the incubation and scaled to represent daily values (mg N kg-1soil d-1). Using our NO3- and NH4+ measures we also calculated potential and realized soil net nitrification and soil net ammonification to be able to better understand the drivers of fertilization and herbivore removal effects on potential and realized soil net Nmin.
A third subsample of the composite soil sample was sieved (2 mm mesh) and microbial biomass (μg Cmic g-1 soil dry weight) was estimated by measuring the maximal respiratory response to the addition of glucose solution (4 mg glucose per g soil dry weight dissolved in distilled water; substrate-induced respiration method) on approximately 5.5 g of soil (Anderson & Domsch, 1978). The rest of the composited sample was dried at 65°C for 48 h, ground and sieved (2 mm mesh) to assess a series of soil chemical properties (Risch et al., 2019). We measured the percentage of clay as an indicator of soil texture (Gee & Bauder, 1986; Risch et al., 2019).
Statistical analyses
Potential and realized soil net Nmin were square root transformed to account for a highly skewed data distribution (yt = sign(y)*sqrt|y|; negative values in the data set impeded log transformation). To assess treatment effects on potential and realized soil net Nmin, we used linear mixed effects models (LMMs) fitted by maximum likelihood using the lme function from the nlme package (version 3.131.1; Pinheiro et al., 2016), R version 3.6.1; R Foundation for Statistical Computing. Treatment (Control, Fence, NPK, NPK+Fence) was a fixed factor, with site and block as random factors, where block was nested within site. We also tested for effects of time since start of treatments in preliminary analyses by adding total treatment years as an additional fixed factor. We did not find a significant effect of years of treatment, and thus dropped this variable from the models. The LMMs were corrected using varIdent if the homogeneity of variance criterion was not met (Pinheiro et al., 2016). To visualize our results, we calculated treatment effects using Cohens’ d statistic (Cohen, 1977; Koricheva et al., 2013). Note that calculating response ratios (or log response ratios) was not possible with our data, as we have both negative and positive values. We also fitted LMMs for potential and realized soil net ammonification and nitrification to gain more insight into how global change affects the processes underpinning potential and realized soil net Nmin. We also sqrt-transformed (see above) these dependent variables. Treatment was included as a fixed factor with random factors as described above. In addition, we assessed how potential and realized soil net Nmin were related to potential and realized soil net ammonification and nitrification, respectively. For this, we calculated site averages for each treatment separately. We then ran LMMs, with potential and realized soil net Nmin as the dependent variable, potential and realized soil net ammonification/nitrification as the independent ones. Site was included as a random factor.
Based on our previous work (Risch et al., 2019) and the existing literature (Schimel & Bennett, 2004; Liu et al., 2017), we developed a priori causal conceptual models of relationships among treatments, environmental drivers, and potential and realized soil net Nmin (Supplementary Fig. S2) to test with structural equation modelling (SEM) using a d-sep approach (Shipley, 2009; Lefcheck, 2016). The variables included in the model were long-term climatic conditions, specifically, site-level mean annual precipitation (MAP) and temperature of the wettest quarter (T.q.wet), plot-level soil texture (clay content) and soil microbial biomass. Mean annual precipitation and T.q.wet were obtained from WorldClim (Hijmans et al., 2005) (http://www.worldclim.org/) and together with the experimental treatments were predicted to directly affect soil properties and soil net Nmin (Supplementary Fig. S2). Soil clay content was, in turn, predicted to affect microbial biomass and soil net Nmin. Because we determined microbial biomass prior to incubating the samples in the laboratory or field, we assumed that the abundance of these microbes would be responsible for N process rates and not vice versa (Supplementary Fig. S2). We tested our conceptual model (Supplementary Fig. S2) using the piecewiseSEM package (version 2.0.2; Lefcheck, 2016) in R 3.4.0, in which a structured set of linear models are fitted individually. This approach allowed us to account for the nested experimental design, and overcome some of the limitations of standard structural equation models, such as small sample sizes (Shipley, 2009; Lefcheck, 2016). We used the lme function of the nlme package to model response variables, including site as a random factor. Good fit of the SEM was assumed when Fisher’s C values were non-significant (p > 0.05). For all significant interactions between covariates and experimental treatments detected in the SEMs, we calculated treatment effect sizes, i.e. the differences in potential or realized soil net Nmin between Control and treatments (Fence, NPK, NPK+Fence) and plotted these values against the climate or soil covariates. Finally, we fitted LMMs for the soil variables included in our SEMs, with treatment as the fixed factor, and with site and block as random factors, where block was nested within site.

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:

Risch Anita C., Zimmermann, Stefan, Moser, Barbara, Schütz, Martin, Hagedorn, Frank, Firn, Jennifer, Fay, Philip A., Adler, Peter B., Biederman, Lori A., Blair, John M., Borer, Elizabeth T., Broadbent, Arthur A.D., Brown, Cynthia S., Cadotte, Marc W., Caldeira, Maria C., Davies, Kendi F., di Virgilio, Augustina, Eisenhauer, Nico, Eskelinen, Anu, Knops, Johannes M.H., MacDougall, Andrew S., McCulley, Rebecca L., Melbourne, Brett A., Moore, Joslin L., Power, Sally A., Prober, Suzanne M., Seabloom, Eric W., Siebert, Julia, Silveira, Maria L. , Speziale, Karina L., Stevens, Carly J., Tognetti, Pedro M., Virtanen, Risto, Yahdjian, Laura, Ochoa-Hueso, Raul (accepted). Global impacts of fertilization and herbivore removal on soil net nitrogen mineralization are modulated by local climate and soil properties. Global Change Biology

Please cite this paper together with the citation for the datafile.

We assessed how the removal of mammalian herbivores (Fence) and fertilization with growth-limiting nutrients (N, P, K, plus nine essential macro- and micronutrients; NPK) individually, and in combination (NPK+Fence), affected potential and realized soil net Nmin across 22 natural and semi-natural grasslands on five continents. Our sites spanned a comprehensive range of climatic and edaphic conditions found across the grassland biome. We focused on grasslands, because they cover 40-50% of the ice-free land surface and provide vital ecosystem functions and services. They are particularly important for forage production and C sequestration. Worldwide, grasslands store approximately 20-30% of the Earth’s terrestrial C, most of it in the soil (Schimel, 1995; White et al., 2000).

ammonification

display_name: ammonification

climate

display_name: climate

fertilization

display_name: fertilization

global-change

display_name: global-change

grassland

display_name: grassland

herbivore

display_name: herbivore

mineralization

display_name: mineralization

nitrification

display_name: nitrification

nitrogen

display_name: nitrogen

nutrient-network

display_name: nutrient-network

soil

display_name: soil

educ

en: Education, culture and sport

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Anthropogenic change and soil net N mineralization


en: beech forest-reserves forest-in temperate-forests swiss-plateau

en: Results trees

en:
The file results_trees.csv contains results of living, standing and lying and dead standing
trees as well as for stumps (trees found on the plot but broken / cut below 1.3 m).

en: Results lying deadwood

en: The file results_lying_deadwood.csv contains results of the lying deadwood survey.

en: Results habitat trees

en: The file results_habitat_trees.csv contains results for the number of habitat trees.

en: Data description

media_type: application/pdf

en: This document briefly describes the datasets.

format: PDF

en: Lookup

en: The file lookup.csv contains description for the values shown in the 5 result tables.

en: Results regeneration

en: The file results_regeneration.csv contains results of the regeneration survey.

en: Results TreMs

en: The file results_trems.csv contains results for the density of TreMs per ha.

en:
The Jorat is one of the largest continously forested areas in the Swiss Plateau. On a forested area of 778 ha, the Parc Naturel du Jorat (PNJ), a periurban parc, has been established in 2022. To document the initial state of the forest within the perimeter of the PNJ, a sample plot inventory (SPI) was carried out on 132 sample plots (SP) in winter 2021/22.
This dataset contains results from this sample plot inventory. It consists of the following files:
- results_trees.csv: Results for or living and dead trees.
- results_regeneration.csv: Results for trees with DBH < 7.0 cm, assessed in three height classes.
- results_lying_deadwood.csv: Results for lying deadwood, assessed no three line transects
- results_trems.csv: Results for occurence of tree related microhabitats (TreMs)
- results_habitat_trees.csv: Results for occurence / densities of trees carrying at least one TreM or with a DBH >= 80 for living trees or >= 36 cm for dead trees respectively.
- lookup.csv: Contains lookup tables which describe the respective results in-depth.
- data_description.pdf: Briefly describes the datasets mentioned above.

beech

display_name: beech

forest-in

display_name: forest-in

forest-reserves

display_name: forest-reserves

swiss-plateau

display_name: swiss-plateau

temperate-forests

display_name: temperate-forests

en: Sample plot inventory data form the Parc Naturel du Jorat


maintainer: EnviDat Support

en: swiss-geodata geoinformation-system digital-elevation-model open-source wms-data cable-yarding qgis-plugin seilaplan digital-planning

en: WMS-Layer als Hintergrundkarten laden

en: View video on youtube!

en:
In order to digitally plan a cable line using the QGIS plugin ‘Seilaplan’, maps with various background information are helpful. In this tutorial we show you how to obtain maps that are helpful for cable line planning, for example a national map of Switzerland at different scales, the NFI vegetation height model or the NFI forest mix rate. For this we explain what WMS datasets are and how to integrate them into QGIS. No download of large data is needed for this, only a good internet connection. Please note that the tutorial language is German!

Link for the integration of WMS data: https://wms.geo.admin.ch/

Link to the description on the Swisstopo website:
https://www.geo.admin.ch/en/geo-services/geo-services/portrayal-services-web-mapping/web-map-services-wms.html

Link to the Seilaplan website: https://seilaplan.wsl.ch

**************************

Für die Verwendung des QGIS Plugins Seilaplan zur digitalen Seillinienplanung sind verschiedene Hintergrundkarten hilfreich. In diesem Tutorialvideo zeigen wir, was WMS Daten sind und wie man diese in QGIS einbinden kann. Dafür müssen die Daten nicht heruntergeladen werden. Es braucht lediglich eine gute Internetverbindung. Für die Seillinienplanung hilfreiche Karten sind bspw. die Landeskarte der Schweiz in verschiedenen Massstäben, das Vegetationshöhenmodell LFI oder der Waldmischungsgrad LFI.

Link zur Einbindung der WMS Daten: https://wms.geo.admin.ch/

Link zur Beschreibung auf der Swisstopo Webseite: https://www.geo.admin.ch/de/geo-dienstleistungen/geodienste/darstellungsdienste-webmapping-webgis-anwendungen/web-map-services-wms.html

Link zur Seilaplan-Website: https://seilaplan.wsl.ch

cable-yarding

display_name: cable-yarding

digital-elevation-model

display_name: digital-elevation-model

digital-planning

display_name: digital-planning

geoinformation-system

display_name: geoinformation-system

open-source

display_name: open-source

qgis-plugin

display_name: qgis-plugin

seilaplan

display_name: seilaplan

swiss-geodata

display_name: swiss-geodata

wms-data

display_name: wms-data

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Seilaplan Tutorial: Load WMS layers as background maps


en: geodata video-encoding raster core research-data-management time-series sentinel-2 gridded-data

en:
Shell script to extract data from the CORE format. You can 1) download and unzip the file, 2) make the script executable and 3) run it.
Disclaimer: only provided for peer review. Proper software will become available in time on https://github.com/EnviDat/?q=core (both Web/JS for Frontend and Python for Backend).

en: CORE Dataset (80 GB)

en:
Shell script to reproduce the CORE format. You can 1) download and unzip the file, 2) make the script executable and 3) run it.
Disclaimer: only provided for peer review. Proper software for working with CORE will become available in time on https://github.com/EnviDat/?q=core (both Web/JS for Frontend and Python for Backend).

en: View publication in the Digital Object Repository at WSL (DORA)

media_type: text/html

format: HTML

en:
DISCLAIMER: CORE is still in development. Interested parties are warmly invited to join common development, to comment, discuss, find bugs, etc.

Acknowledgement: The CORE format was proudly inspired by the Cloud Optimized GeoTIFF ([COG](https://www.cogeo.org/)) format, by considering how to leverage the ability of clients issuing HTTP GET range requests for a time-series of remote sensing and aerial imagery (instead of just one image).

License: The Cloud Optimized Raster Encoding (CORE) specifications are released to the public domain under a Creative Commons 1.0 CC0 "No Rights Reserved" international license. You can reuse the information contained herein in any way you want, for any purposes and without restrictions.

-----------------------

Summary:
The Cloud Optimized Raster Encoding (CORE) format is being developed for the efficient storage and management of gridded data by applying video encoding algorithms. It is mainly designed for the exchange and preservation of large time series data in environmental data repositories, while in the same time enabling more efficient workflows on the cloud. It can be applied to any large number of similar (in pixel size and image dimensions) raster data layers. CORE is not designed to replace COG but to work together with COG for a collection of many layers (e.g. by offering a fast preview of layers when switching between layers of a time series).

WARNING: Currently only applicable to RGB/Byte imagery. The final CORE specifications may probably be very different from what is written herein or CORE may not ever become productive due to a myriad of reasons (see also 'Major issues to be solved').

With this early public sharing of the format we explicitly support the Open Science agenda, which implies "shifting from the standard practices of publishing research results in scientific publications towards sharing and using all available knowledge at an earlier stage in the research process" (quote from: European Commission, Directorate General for Research and Innovation, 2016. Open innovation, open science, open to the world).

CORE Specifications:

1) a MP4 or WebM video digital multimedia container format (or any future video container playable as HTML video in major browsers)

2) a free to use or open video compression codec such as H.264, VP9, or AV1 (or any future video codec that is open sourced or free to use for end users)

Note: H.264 is currently recommended because of the wide usage with support in all major browsers, fast encoding due to acceleration in hardware (which is currently not the case for AV1 or VP9) and the fact that MPEG LA has allowed the free use for streaming video that is free to the end users. However, please note that H.264 is restricted by patents and its use in proprietary or commercial software requires the payment of royalties to [MPEG LA](https://www.mpegla.com/programs/avc-h-264/). However, when AV1 matures and accelerated hardware encoding becomes available, AV1 is expected to offer 30% to 50% smaller file size in comparison with H.264, while retaining the [same quality](https://trac.ffmpeg.org/wiki/Encode/AV1).

3) the encoding frame rate should be of one frame per second (fps) with each layer segmented in internal tiles, similar to COG, ordered by the main use case when accessing the data: either layer contiguous or tile contiguous;

Note: The internal tile arrangement should support an easy navigation inside the CORE video format, depending on the use case.

4) a CORE file is optimised for streaming with the moov atom at the beginning of the file (e.g. with -movflags faststart) and optional additional optimisations depending on the codec used (e.g. -tune fastdecode -tune zerolatency for H.264)

5) metadata tags inside the moov atom for describing and using geographic image data (that are preferably compatible with the [OGC GeoTIFF standard](https://www.ogc.org/standards/geotiff) or any future standard accepted by the geospatial community) as well as list of original file names corresponding to each CORE layer

6) it needs to encode similar source rasters (such as time series of rasters with the same extent and resolution, or different tiles of the same product; each input raster should be having the same image and pixel size)

7) it provides a mechanism for addressing and requesting overviews (lower resolution data) for a fast display in web browser depending on the map scale (currently external overviews)

Major issues to be solved:

- Internal overviews (similar to COG), by chaining lower resolution videos in the same MP4 container for fast access to overviews first); Currently, overviews are kept as separate files, as external overviews.

- Metadata encoding (how to best encode spatial extent, layer names, and so on, for each of the layer inside the series, which may have a different geographical extent, etc...; Known issues: adding too many tags with FFmpeg which are not part of the standard MP4 moov atom; metadata tags have a limited string length.

- Applicability beyond RGB/Byte datasets; defining a standard way of converting cell values from Int16/UInt16/UInt32/Int32/Float32/Float64/ data types into multi-band Byte values (and reconstructing them back to the original data type within acceptable thresholds)

Example

Notice: The provided CORE (.mp4) examples contain modified Copernicus Sentinel data [2018-2021].

For generating the CORE examples provided, 50 original Sentinel 2 (S-2) TCI data images from an area located inside Switzerland were downloaded from www.copernicus.eu, and then transformed into CORE format using ffmpeg with H.264 encoding using the [x264 library](https://www.videolan.org/developers/x264.html).

For full reproducibility, we provide the original data set and results, as well scripts for data encoding and extraction (see resources).

core

display_name: core

geodata

display_name: geodata

gridded-data

display_name: gridded-data

raster

display_name: raster

research-data-management

display_name: research-data-management

time-series

display_name: time-series

video-encoding

display_name: video-encoding


maintainer: Jana von Freyberg

en: endmember-mixing-models review plant-water-uptake uncertainties root-zone ecohydrology stable-water-isotopes

en: Data set of MEE-20-04-264 (CSV)

media_type: text/csv

format: CSV

en: Data set of MEE-20-04-264

en: The following two tables contain information about the data sources of the values reported in Table 1 and 2 in the paper “Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources” published in the journal ‘Methods in Ecology and Evolution’.

mimetype: application/pdf

en: The following two tables contain information about the data sources of the values reported in Table 1 and 2 in the paper “Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources” published in the journal 'Methods in Ecology and Evolution'.

ecohydrology

display_name: ecohydrology

endmember-mixing-models

display_name: endmember-mixing-models

plant-water-uptake

display_name: plant-water-uptake

review

display_name: review

root-zone

display_name: root-zone

stable-water-isotopes

display_name: stable-water-isotopes

uncertainties

display_name: uncertainties

en: Data set of: Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources