maintainer: Anita Risch

en: grassland ecosystem-multifunctionality ecosystem-functions invertebrates vertebrates swiss-national-park exclusion subalpine grazing ecosystem-coupling

en: Datafile

en:
ID: Plot ID composited of Fence & Treatment number (90 plots total)

Grassland: Name of the six subalpine grasslands where the exclosure setups were erected. In large grasslands (GRIM, MING, STAB), we established four hierarchical exclosure setups (two in short- and two in tall-grass vegetation), whereas in the small grasslands (BOTS, DADA, MARG) only one setup was erected per vegetation type. BOTS = Val dal Botsch, GRIM = Grimmels, MING = Val Mingér, STAB = Alp Stabelchod, DADA = Stabelchod da daint, MARG = Margunet

Fence: Number of exclosure setup (1 - 18). Note that uneven numbers (1,3,5,7,9,11,13,15,17) represent setups established on short-grass vegetation, even numbers (2,4,6,8,10,12,14,16,18) setups established in tall-grass vegetation
Vegetation	Vegetation type, 1 = short-grass vegetation, 2 = tall-grass vegetation; (9 replicate fences on each vegetation type)
Treatment	Progressive animal exclusion, numbers indicate which animals had access to the plot: 1 = Large/medium/small mammals, and invertebrates have access ("L/M/S/I"), 2 = Medium/small mammals, and invertebrates have access (“M/S/I”), 3 = Small mammals and invertebrates have access (“S/I”), 4 = Invertebrates have access (“I”), 5 = No animals have access (“None”)

Bulkden:	Fine fraction soil bulk density (g cm-3)

Soil.moist	: Soil moisture (%)

Soil.org.C: Rhizosphere soil C content (%)

pH: Soil pH

Nema.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil nematode community composition

Nema.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil nematode community composition

Micro.PC1: 	First principal component (PC1) of principal component analysis (PCA) of the soil microbial community composition

Micro.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil microbial community composition

Abv.Inv.PC1: First principal component (PC1) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Abv.Inv.PC2: Second principal component (PC2) of principal component analysis (PCA) of the aboveground-dwelling invertebrate community composition

Arthr.PC1: First principal component (PC1) of principal component analysis (PCA) of the soil arthropod community composition

Arthr.PC2: Second principal component (PC2) of principal component analysis (PCA) of the soil arthropod community composition

Plant.PC1: First principal component (PC1) of principal component analysis (PCA) of the plant community composition

Plant.PC2: Second principal component (PC2) of principal component analysis (PCA) of the plant community composition

Root.biomass: Root biomass (g m-2)

Micro.biomass	: Microbial biomass carbon (mg kg-1)

Plant.richness: Plant species richness

Plant.N: Plant tissue nitrogen content (%)

Soil.N.min: Soil net nitrogen mineralisation (kg ha-1 season)

Soil.resp: Soil carbon dioxide emissions (soil respiration; g m-2 h-1)

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:
 > Risch AC, Ochoa-Hueso R, van der Putten WH, Bump JK, Busse MD, Frey B, Gwiazdowicz DJ, Page-Dumroese DS, Vandegehuchte ML, Zimmermann S, Schütz M. Size-dependent loss of aboveground animals differentially affects grassland ecosystem coupling and functions. 2018. Nature Communications 9: 3684. [doi: 10.1038/s41467-018-06105-4](https://doi.org/10.1038/s41467-018-06105-4).

Please cite this paper together with the citation for the datafile.

#Methods
Study sites
The experimental exclosure setups were installed within the SNP (IUCN category Ia preserve; Dudley 2008), in south-eastern Switzerland. The park covers 172 km2 of forests and subalpine and alpine grasslands along with scattered rock outcrops and scree slopes. The entire area has been protected from human impact (no hunting, fishing, camping or off-trail hiking) since 1914. Large, fairly homogenous patches of short- and tall-grass vegetation, which originate from different historical management and grazing regimes, cover the park’s subalpine grasslands entirely. Short-grass vegetation developed in areas where cattle used to rest (nutrient input) prior to the park’s foundation (14th century to 1914) (Schütz and others 2003, 2006) and is dominated by lawn grass species such as Festuca rubra L., Briza media L. and Agrostis capillaris L. (Schütz and others 2003, 2006). Today, this vegetation type is intensively grazed by diverse vertebrate and invertebrate communities that inhabit the park and consume up to 60% of the available biomass (Risch and others 2013). Tall-grass vegetation developed where cattle formerly grazed, but did not rest, and is dominated by rather nutrient-poor tussocks of Carex sempervirens Vill. and Nardus stricta L. (Schütz and others 2003, 2006). This vegetation type receives considerably less grazing, with only roughly 20% of the biomass consumed (Risch and others 2013). Consequently, the two vegetation types together represent a long-term trajectory of changes in grazing regimes. Underlying bedrock of all grasslands is dolomite, which renders these grasslands rather poor in nutrients regardless of former and current land-use regimes.
Experimental design
To progressively exclude aboveground vertebrate and invertebrate animals, we established 18 size-selective exclosure setups (nine in short-grass, nine in tall-grass vegetation) distributed over six subalpine grasslands across the SNP (Risch and others 2013, 2015). Elevation differences of exclosure locations did not exceed 350 m (between 1975 and 2300 m a.s.l.). The exclosures were established immediately after snowmelt in spring 2009 and were left in place for five consecutive growing seasons (until end of 2013). They were, however, temporarily dismantled every fall (late October after first snowfall) to protect them from avalanches. They were re-established in the same location every spring immediately after snowmelt. Each size-selective exclosure setup consisted of five plots (2 x 3 m) that progressively excluded aboveground vertebrates and invertebrates from large to small. The plots are labelled according to the guilds that had access to them “L/M/S/I”, “M/S/I”, “S/I”, “I”, “None”; L = large mammals, M = medium mammals, S = small mammals, I = invertebrates, None = no animals had access. As we only had permission to have the experimental setup in place for five consecutive growing seasons, the experiment had to be completely dismantled in the late fall of 2013 and all material removed from the SNP.
Our exclosure design was aimed at excluding mammalian herbivores, but naturally also excluded the few medium and small mammalian predators, as well as the entire aboveground invertebrate food web. A total of 26 large to small mammal species can be found in the SNP, but large apex predators are missing (wolf, bear, lynx) . Reptiles, amphibians and birds are scarce to absent in the subalpine grasslands under study. Only two reptile species occur in the park and they are confined to rocky areas that warm up enough for them to survive. One frog species spawns in an isolated pond far from our grasslands. Only three bird species occasionally feed on the subalpine grasslands. Using game cameras (Moultrie 6MP Game Spy I-60 Infrared Digital Game Camera, Moultrie Feeders, Alabaster, AL, USA), we did observe that the medium- and small-sized mammals (marmot/hares and mice) were not afraid to enter the fences and feed on their designated plots. We never spotted reptiles, amphibians or birds on camera. We distinguished between 59 higher aboveground-dwelling invertebrate taxa that our size-selective exclosures excluded (see also methods for aboveground-dwelling invertebrates below).
The “L/M/S/I” plot (not fenced) was located at least 5 m from the 2.1 m tall and 7 x 9 m large main electrical fence that enclosed the other four plots. The bottom wire of this fence was mounted at 0.5 m height and was not electrified to enable safe access for medium and small mammals, while fencing out the large ones. Within each main fence, we randomly established four 2 x 3 m plots separated by 1-m wide walkways from one another and from the main fence line: 1) the “M/S/I” plots were unfenced, allowing access to all but the large mammals; 2) the “S/I” plots (10 x 10 cm electrical mesh fence) excluded all medium-sized mammals. Note that the bottom 10 cm of this fence remained non-electrified to enable safe access for small mammals; 3) the “I” plots (2 x 2 cm metal mesh fence) excluded all mammals. We double-folded the mesh at the bottom 50 cm to reduce the mesh size to smaller than 1 x 1 cm openings; and 4) the “None” plots were surrounded by a 1 m tall mosquito net (1.5 x 2 mm) to exclude all animals. The top of the plot was covered with a mosquito-meshed wooden frame mounted to the corner posts (roof). We treated these plots a few times with biocompatible insecticide (Clean kill original, Eco Belle GmbH, Waldshut-Tiengen, Germany) to remove insects that might have entered during data collection or that hatched from the soil, but amounts were negligible and did not impact soil moisture conditions within these plots.
To assess whether the design of the “None” exclosure (mesh and roof) affected the response variables within the plots and, therefore, influenced the results, we established an additional six “micro-climate control” exclosures (one in each of the six grasslands) (Risch and others 2013, 2015). These exclosures were built as the “None” exclosures but were open at the bottom (20 cm) of the 3-m side of the fence facing away from the prevailing wind direction to allow invertebrates to enter. A 20-cm high and 3-m long strip of metal mesh was used to block access to small mammals. Thus, this construction allowed a comparable micro-climate to the “None” plots, but also a comparable feeding pressure by invertebrates to the “I” plots. We compared various properties within these exclosures against one another to assess if our construction altered the conditions in the “None” plots. We showed that differences in plant (e.g., vegetation height, aboveground biomass) and soil properties (e.g., soil temperature, moisture) found between the “I” and the “None” treatments were not due to the construction of the “None” exclosure, but a function of animal exclusions, although the amount of UV light reaching the plant canopy was significantly reduced (Risch and others 2013).
Aboveground invertebrate sampling
Aboveground invertebrates were sampled with two different methods to capture both ground- and plant-dwelling organisms: 1) we randomly placed two pitfall traps (67 mm in diameter, covered with a roof) filled with 20% propylene glycol in one 1 x 1 m subplot of the 2 x 3 m treatment plots in spring 2013 (May) and emptied them every two weeks until late September 2013 (Vandegehuchte and others 2017b, 2018). A pitfall trap consisted of a plastic cylinder (13 cm depth, 6.75 cm diameter). Within each cylinder we placed a 100 ml plastic vial with outer diameter 6.70 cm and on top of the cylinder we placed a plastic funnel to guide the invertebrates into the vials. Each trap was cover with a cone-shaped and transparent plastic roof to protect the trap from rain (Vandegehuchte and others 2017b, 2018). Note that in the “None” plots only one trap was placed as control to check for effectiveness of the exclosure. 2) We vacuumed all invertebrates from a 60 x 60 cm area on another 1 x 1 m subplot with a suction sampler (Vortis, Burkhard manufacturing CO, Ltd., Rickmansworth, Hertfordshire, UK) every month from June to September 2013 (Vandegehuchte and others 2017b, 2018). For this purpose, we quickly placed a square plastic frame (60 x 60 x 40 cm) with a closable mosquito mesh sleeve attached to the top edge into the plot from the outside. The suction sample was then inserted into through the sleeve and operated for 45 s to collect the invertebrates (Vandegehuchte and others 2017b, 2018).
We sorted the ≈100 000 individuals collected with both methods by hand and identified each individual morphologically to the lowest taxonomic level feasible (59 taxa, including orders, suborders, subfamilies, families; phylum for Mollusca). These taxa belonged to the following feeding types: 19 herbivores, 16 detritivores, 9 predators, 8 mixed feeders, 5 omnivores and 2 non-classified feeders (or not feeding as adults) (Vandegehuchte and others 2017b). We summed the numbers from the two pitfall traps and the suction sampling over the course of the 2013 season to represent the aboveground invertebrate abundance and community composition of a plot. Note: we did not specifically attempt to catch flying invertebrates with e.g., sticky traps, thus a few flying insects may have been missed with our vacuum sampling approach.
Sampling of plant properties
The vascular plant species composition was assessed at peak biomass every summer (July) by estimating the frequency of occurrence of each species with the pin count method in each plot (Frank and McNaughton 1990). A total of 172 taxa occurred within our 90 plots and we calculated plant species richness for each plot separately. We used the 2013 data in this study. Plant quality was assessed every year in July and September; here we use plant quality at the end of the experiment (September 2013). Two 10 x 100 cm wide strips of vegetation per plot were clipped, combined, dried at 65°C, and ground (Pulverisette 16, Fritsch, Idar-Oberstein, Germany) to pass through a 0.5 mm sieve. Twenty randomly selected samples across all treatments were analysed for N (Leco TruSpec Analyser, Leco, St. Joseph, Michigan, USA) (Vandegehuchte and others 2015). Nitrogen concentrations of the other samples were then estimated from models established for the experiment and the entire SNP relating Fourier transform-near infrared reflectance (FT-NIR) spectra to the measured values of N using a multi-purpose FT-NIR spectrometer (Bruker Optics, Fällanden, Switzerland) (Vandegehuchte and others 2015). Root biomass was sampled every fall by collecting five 2.2 cm diameter x 10 cm deep soil samples (Giddings Machine Company, Windsor, CO, USA) per plot (450 samples year-1). The samples were dried at 30 °C and roots were sorted from the sample by hand. We sorted each sample for 1 h which allowed to retrieve over 90% of all roots present in the samples (Risch and others 2013). The roots were then dried at 65 °C for 48 and weighed to the nearest mg. We averaged the values per plot and used the 2013 data only in this study.
Sampling of edaphic communities
In 2009, 2010, and 2011 we collected three composited soil samples (5 cm diameter x 10 cm depth; AMS Samplers, American Falls, ID, USA) and assessed bacterial community structure using T-RFLP profiling (Liu and others 1997; Blackwood and others 2003; Hodel and others 2014). We detected a total of 89 operational taxonomic units (OTUs). These values are in accordance with other studies reporting OTU richness (Wirthner and others 2011; Zumsteg and others 2012; Meola and others 2014) using T-RFLP profiling, a method that detects the most abundant, and thus likely, the most relevant, taxa. We averaged the data over the three years of collections for our calculations. Microbial biomass carbon (MBC) was determined with the substrate-induced method (Anderson and Domsch 1978) every fall (September) between 2009 and 2013 by collecting three mineral soil samples (5 cm diameter × 10 cm mineral soil core, AMS Samplers, American Falls, ID, USA). The three samples were combined (90 samples for each sampling year), immediately put on ice, taken to the laboratory, passed through a 2-mm sieve and stored at 4°C. Again, we only used the 2013 data in this study.
Soil samples (5 cm diameter x 10 cm depth) to extract soil arthropods were collected in June, July, and August 2011 with a soil corer lined with a plastic sleeve to ensure an undisturbed sample (total of 270 samples). The plastic line core was immediately sealed on both ends using cling film and put into a cooler. All plots were sampled within three days and the extraction of arthropods started the evening of the sampling day using a high-gradient Tullgren funnel apparatus (Crossley and Blair 1991; Vandegehuchte and others 2015). Samples were kept in the extractor for four days and the soil arthropods were collected in 95% ethanol. All individuals were counted and each individual was identified morphologically to the lowest level feasible [76 taxa, including orders, suborders, subfamilies, families (Protura, Thysanoptera, Aphidina, Psylina, Coleoptera, Brachycera, Nematocera, Auchenorryncha, Heteroptera, Formicidae); sub-phylum for Myriapoda, for Acari and Collembola also including morpho-species). Note that we also included larval stages (nine of the 76 taxa) (Vandegehuchte and others 2015). All data were summed over the season. A detailed species list for mites and collembolans is published (Vandegehuchte and others 2017a) [https://doi.org/10.1371/journal.pone.0118679.s001]. Earthworms are rare in the SNP and therefore were not included. We collected eight random 2.2 cm diameter x 10 cm deep soil cores from each plot in September 2013 to determine the soil nematode community composition. The samples were mixed and the nematodes were extracted from 100 ml of fresh soil using Oostenbrink elutriators (Oostenbrink 1960). All nematodes in a 1 ml of the 10 ml extract were counted, a minimum of 150 individuals sample-1 were identified to genus or family level using (Bongers 1988), the numbers of all nematodes were extrapolated to the entire sample and expressed for a 100 g dry sample. In total we identified 63 genus or family levels (Vandegehuchte and others 2015). The list of all the nematodes found is published (Vandegehuchte and others 2015) [http://www.oikosjournal.org/appendix/oik-03341] or DOI: [doi: 10.1111/oik.03341].
We are aware that sampling soil microbes from 2009 to 2011 and soil arthropods in 2011 was not ideal, but we are positive that this does not bias the results. Most of the parameters measured in our experiment either already showed a treatment response after the first growing season (e.g., plant biomass) or did not respond over the entire time experiment (e.g., microbial biomass C). The microbial community composition (2009 – 2011) was highly influenced by inter-annual differences in temperature and precipitation, but did not differ between treatments or vegetation types (Hodel and others 2014). We therefore felt comfortable using the 2009 through 2011 data for describing the soil microbial community in our experimental treatments. Similarly, we are positive that our soil arthropod data are representative. We did assess soil arthropods in August 2012 and found no differences to the August 2011 data. However, we did not feel comfortable combining the 2011 June, July, August data with only August data for 2012 for our analyses.
Sampling of soil properties
We collected three soil samples (5 cm diameter x 10 cm depth) in each plot in September 2013 after removing the vegetation. First, we collected the top layer of mineral soil rich in organic matter, the surface organic layer or rhizosphere, typically 1 to 3 cm in depth with a soil corer (AMS Samples, American Falls, Idaho, USA). Second, we collected a 10 cm mineral soil core beneath this surface layer. The cores for each layer were composited, dried at 65 °C for 48 h and fine-ground to pass a 0.5 mm screen. We then analysed all samples for total C using a Leco TruSpec Analyser (Leco, St. Joseph, Michigan, USA). Mineral soil pH was measured potentiometrically in 1:2 soil:CaCl2 solution with an equilibration time of 30 min.
Soil net N mineralisation was assessed during the 2013 growing season (Risch and others 2015). For this purpose, we randomly collected a 5 cm diameter x10 cm deep soil sample with a soil corer (AMS Samples, American Falls, Idaho, USA) after clipping the vegetation in June 2013. After weighing and sieving (4 mm mesh) the soil, we extracted a 20 g subsample in 1 mol l-1 KCl for 1.5 h on an end-over-end shaker and thereafter filtered it through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnenmühle FineArt GmbH, Dassel, Germany). From these filtrates NO3- concentrations were measured colorimetrically (Norman and Stucki 1981) and NH4+with flow injection analysis (FIAS 300, Perkin Elmer, Waltham Massachusetts, USA) (Risch and others 2015). We dried the rest of the sample 105 °C to constant mass to determine fine,fraction bulk density. A second soil sample was collected within each plot in June 2013 with a corer lined with a 5 x 13 cm aluminium cylinder. The corer was driven 11.5 cm deep into the soil so that the top 1.5 cm of the cylinder remained empty. Into this space we placed a polyester bag (250 µm) filled an ion-exchanger resin to capture the incoming N. The bag was filled with a 1:1 mixture of acidic and alkaline exchanger resin (ion-exchanger I KA/ion exchanger IIIAA, Merck AG, Darmstadt, Germany). We then removed 1.5 cm soil at the bottom of the cylinder and placed a second resin exchanger bag into this space to capture the N leached from the soil column. To assure that the exchange resin was saturated with H+ and Cl- prior to filling the bags, the mixture was stirred with 1.2 ml l-1 HCl for 1 h and then rinsed with demineralized water until the electrical conductivity of the water reached 5 µm cm-1. The cylinder with the resin bags in place was reinserted into the soil with the top flush to the soil surface and incubated for three months. We recollected the cylinders in September 2013. Each resin bag and 20 g of sieved soil (4 mm mesh) from each cylinder were then separately extracted with KCl and NO3- and NH4+ concentrations were measured. Nitrate and NH4+ concentrations of all samples were then converted to a content basis by multiplying their values with fine fraction bulk density. Net N mineralisation was thereafter calculated as the difference between the N content of the samples collected at the end of the three-month incubation (including the N extracted from the bottom resin bag) and the N content at the beginning of the incubation (Risch and others 2015).
Soil CO2 emissions were measured every two weeks between 0900 and 1700 hrs from early May through late September 2013 with a PP-Systems SRC-1 soil respiration chamber (15 cm high, 10 cm diameter; closed circuit) attached to a PP-Systems EGM-4 infrared gas analyser (PP-Systems, Amesbury, MA, USA) on two locations per plot (Risch and others 2013). The chamber was placed on randomly placed, permanently installed PVC collars (10 cm diameter) driven 5 cm into the soil at the beginning of the study (Risch and others 2013). Freshly germinated plants growing within the collars were removed prior to each measurement to avoid measuring plant respiration or photosynthesis. The two measurements collected per plot and sampling date were averaged.
Soil moisture (with time domain reflectometry; Field-Scout TDR-100, Spectrum Technologies, Plainfield, Illionois, USA) and temperature (with a waterproof digital pocket thermometer; Barnstead International, Dubuque, Iowa, USA) were measured at five random locations per plot every two weeks during the growing seasons during the experiment for the 0 to 10 cm depth (Risch and others 2013, 2015). As soil moisture and soil temperature were highly negatively correlated (Risch and others 2013), we only used soil moisture for this study. We used plot-level averages of all values available to capture soil moisture variability during the five years of the experiment. The results remained unchanged when we only used soil moisture from the 2013 growing season.
Numeral calculations and statistical analyses
Ecosystem coupling. We conducted principal component analyses (PCAs; unscaled) at the complete dataset level using the abundances of each taxonomical entity to describe each of the five different communities used in this study: aboveground-dwelling invertebrates, vascular plants, soil microorganisms, soil arthropods and soil nematodes. We retained the first two components (PCA axis 1 and PCA axis 2) of each analysis as we found them to adequately represent the temporal and spatial variability of our 90 treatment plots in previous studies55,67. Together they explained a total of 71.70% of the variation for aboveground invertebrates, 44.36% for plants, 44.85% for soil microorganisms, 61.85% for soil arthropods and 77.19% for soil nematodes. In addition, we used soil pH and soil organic C content as a proxy for soil chemical properties, soil bulk density as a proxy for soil physical properties and soil moisture (negatively correlated with soil temperature) as a proxy for soil micro-climatic conditions for an overall total of fourteen constituents.
We calculated ecosystem coupling9 for each exclosure treatment within each vegetation type (i.e., 2  5 treatment combinations in total) as an integrated measure of pairwise ecological interactions between ecosystem constituents representing ecological communities and the soil abiotic environment. These ecological interactions are defined by non-parametric Spearman rank correlation analyses between two constituents, excluding interactions involving two abiotic constituents (e.g., soil pH vs. soil moisture) and interactions between the first (PC1) and second (PC2) component of each community type, as these are orthogonal by definition. Interactions between abiotic constituents were excluded from the analyses because the focus of our study was on communities and how they interact with one another and their surrounding environment; therefore, including abiotic-abiotic interactions was not of interest here. Given that the effectiveness of our experimental design resulted in that no community composition data of aboveground-dwelling invertebrates was available for the “None” plots (all animals excluded), only thirteen instead of fourteen constituents were included in the ecosystem coupling calculations for this treatment. The complete absence of aboveground invertebrates represents the most extreme case of disturbance between aboveground animal communities and the rest of the ecosystem constituents. This may have resulted in a slight overestimation of ecosystem coupling for these plots.
	Average ecosystem coupling was calculated as follows:
Ecosystem coupling=
where Xi is the absolute Coupling was calculated value of the Spearman’s rho coefficient of the ith correlation for each treatment within each vegetation type (i.e., based on nine replicates each), considering and n is the number of pairwise comparisons considered (n = a total of 80; interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).	Coupling was calculated for each treatment within each vegetation type (i.e., based on nine replicates each), considering a total of 80 interactions (56 in the case of the “None” treatment). We considered a total of 40 biotic-biotic interactions (i.e., concerning two community-level principal components such as plants and microbes; 24 in the case of the “None” treatment) and 40 abiotic-biotic (i.e., concerning one community-level principal component and one abiotic factor, e.g., plant community and soil properties; 32 in the case of the “None” treatment).
To establish whether constituents were significantly and positively coupled within treatments (i.e., the average of their correlation coefficients were greater than in a null model where correlation only happens by chance), we calculated one-tailed p-values based on permutation tests with 999 permutations.

We considered six ecosystem functions and process rates commonly used to assess ecosystem functioning (Meyer and others 2015; Manning and others 2018). Plant N content represents a measure of forage quality, while plant richness has been shown to stabilise biomass production, thus allowing the system to respond to changes in herbivory. Soil net N mineralisation, soil respiration, root biomass, and microbial biomass represent fluxes or stocks of energy. For all functions and processes higher values represent higher functioning (Manning and others 2018). All these variables were measured in the last year of the experiment (2013). We then quantified ecosystem multifunctionality using the multiple threshold approach (Byrnes and others 2014; Manning and others 2018), which considers the number of functions that are above a certain threshold, over a series of threshold values (typically 10-99%) that are defined based on the maximum value of each function. We weighted all our functions equally for these calculations (Manning and others 2018). The number of functions in a plot with values higher than a given threshold value for the respective function is summed up. The sum represents ecosystem multifunctionality for that plot. Given that choosing any particular threshold as a measure of ecosystem multifunctionality is arbitrary, we calculated the average of thresholds from 10-90% (in 10% intervals) as a more integrated representation of ecosystem multifunctionality.
We used Pearson correlations to explore the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and ecosystem multifunctionality by calculating the slopes of all relationships between ecosystem coupling and multifunctionality for all thresholds between 10 and 99%. We also related ecosystem coupling with the average of multifunctionality at thresholds between 30-80% as explained before and considered this correlation as a robust indication of the type of association between these two variables. In addition, we explored the relationships between ecosystem coupling (all interactions, biotic-biotic interactions, abiotic-biotic interactions involving above- and belowground constituents, and all interactions, biotic-biotic interactions, abiotic-biotic interactions involving belowground constituents only) and individual ecosystem functions. The effects of exclosures and vegetation type on individual functions and multifunctionality were evaluated using linear mixed effects models ('lme' function of the nlme package), with exclosure and vegetation type as fixed effects and fence as a random factor. All statistical analyses and numerical calculations were done in R version 3.4.0 (R Core Team 2016).

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- R Core Team. 2016. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing
- Risch AC, Haynes AG, Busse MD, Filli F, Schütz M. 2013. The response of soil CO2 fluxes to progressively excluding vertebrate and invertebrate herbivores depends on ecosystem type. Ecosystems 16:1192–202.
- Risch AC, Schütz M, Vandegehuchte ML, Van Der Putten WH, Duyts H, Raschein U, Gwiazdowicz DJ, Busse MD, Page-Dumroese DS, Zimmermann S. 2015. Aboveground vertebrate and invertebrate herbivore impact on net N mineralization in subalpine grasslands. Ecology 96:3312–22.
- Schütz M, Risch AC, Achermann G, Thiel-Egenter C, Page-Dumroese DS, Jurgensen MF, Edwards PJ. 2006. Phosphorus translocation by red deer on a subalpine grassland in the Central European Alps. Ecosystems 9:624–633.
- Schütz M, Risch AC, Leuzinger E, Krüsi BO, Achermann G. 2003. Impact of herbivory by red deer (Cervus elaphus L.) on patterns and processes in subalpine grasslands in the Swiss National Park. For Ecol Manage 181:177–88.
- Vandegehuchte ML, van der Putten WH, Duyts H, Schütz M, Risch AC. 2017a. Aboveground mammal and invertebrate exclusions cause consistent changes in soil food webs of two subalpine grassland types, but mechanisms are system-speciﬁc. Oikos 126:212–23.
- Vandegehuchte ML, Raschein U, Schütz M, Gwiazdowicz DJ, Risch AC. 2015. Indirect short- and long-term effects of aboveground invertebrate and vertebrate herbivores on soil microarthropod communities. PLoS One 10:e0118679.
- Vandegehuchte ML, Schütz M, de Schaetzen F, Risch AC. 2017b. Mammal-induced trophic cascades in invertebrate food webs are modulated by grazing intensity in subalpine grassland. J Anim Ecol 86:1434–46.
- Vandegehuchte ML, Trivellone V, Schütz M, Firn J, de Schaetzen F, Risch AC. 2018. Mammalian herbivores affect leafhoppers associated with specific plant functional types at different timescales. Funct Ecol 32:545–55.
- Wirthner S, Frey B, Busse MD, Schütz M, Risch AC. 2011. Effects of wild boar (Sus scrofa L.) rooting on the bacterial community structure in mixed-hardwood forest soils in Switzerland. Eur J Soil Biol 47:296–302. http://dx.doi.org/10.1016/j.ejsobi.2011.07.003
- Zumsteg A, Luster J, Göransson H, Smittenberg RH, Brunner I, Bernasconi SM, Zeyer J, Frey B. 2012. Bacterial, Archaeal and Fungal Succession in the Forefield of a Receding Glacier. Microb Ecol 63:552–64. https://doi.org/10.1007/s00248-011-9991-8

ecosystem-coupling

display_name: ecosystem-coupling

ecosystem-functions

display_name: ecosystem-functions

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

exclusion

display_name: exclusion

grassland

display_name: grassland

grazing

display_name: grazing

invertebrates

display_name: invertebrates

subalpine

display_name: subalpine

swiss-national-park

display_name: swiss-national-park

vertebrates

display_name: vertebrates

educ

en: Education, culture and sport

en: Ecosystem coupling and multifunctionality - exclosure experiment


maintainer: EnviDat Support

en: mars snow sediment-transport erosion model

state: active

type: dataset

media_type: text/plain

en: Datasets of Figure 4b

format: TXT

mimetype: text/plain

en: Datasets of Figure 3a

en: Datasets of Figure 3b

en: Datasets of Figure 2

en: Datasets of Figure 4a

en:
The files contain the datasets used to produce Figures 2, 3, and 4 of the manuscript ([doi: 10.1002/2016GL071822](http://dx.doi.org/10.1002/2016GL071822)).

 Manuscript Abstract:
Despite being the main sediment entrainment mechanism in aeolian transport, granular splash is still poorly understood. We provide a deeper insight into the dynamics of sand and snow ejection with a stochastic model derived from the energy and momentum conservation laws. Our analysis highlights that the ejection regime of uniform sand is inherently different from that of heterogeneous sand. Moreover, we show that cohesive snow presents a mixed ejection regime, statistically controlled either by energy or momentum conservation depending on the impact velocity. The proposed formulation can provide a solid base for granular splash simulations in saltation models, leading to more reliable assessments of aeolian transport on Earth and Mars.

erosion

display_name: erosion

mars

display_name: mars

model

display_name: model

sediment-transport

display_name: sediment-transport

snow

display_name: snow

fr: Éducation, culture et sport

de: Bildung, Kultur und Sport

it: Istruzione, cultura e sport

envidat

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

en: EnviDat: the Environmental Data Portal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: Energy- and momentum-conserving model of splash entrainment in sand and snow saltation


en: pest-invasion southern-switzerland dryocosmus-kuriphilus castanea-sativa population-evolution biological-control torymus-sinensis

media_type: text/csv

en:
Data has been collected from summer 2011 to 2016.

Main reasons for NA values:

-	D. kuriphilus still not arrived in the site.
-	No galls were found during the field sampling.
-	Site has not been sampled in the correspondent year because of time, weather or money constrains.
-    T. sinensis not found in the galls

Variable description:

Year = sampling year.

Coordinate, elevation, slope, aspect = average site characteristics.

ts_arrival = T. sinensis arrival year.

dk_arrival = D. kuriphilus arrival year.

dk_parasitism_by_ts = D. kuriphilus parasitism rate by T. sinensis. Formula: (no. of T. sinensis/ no. of D. kuriphilus chambers) * 100 at gall level.

dk_infestation = Percentage of attacked buds. Formula: no. of attacked buds / no. of total developed buds*100 (as presented in Kotobuki et al. 1985) at shoot level.

ts_per_bud = number of alive T. sinensis individual calculated at bud level.

dk_per_bud = number of alive D. kuriphilus individual calculated at bud level.

dk_pot_per_bud = overall number (dead and alive) D. kuriphilus individual calculated at bud level.

total_gall_nr_per_bud = average gall number per bud.

chamber_per_galls = average D. kuriphilus chamber number per gall.

For details see the submitted publication with the same name

References
Kotobuki, K., Mori, K. and Sato, Y. (1985) ‘2 methods to estimate the tree damage by chestnut gall wasp Dryocosmus-kuriphilus’, Bulletin of the fruit tree research station A (Yatabe), 2(12), pp. 29–36.

format: CSV

mimetype: text/csv

en:
This dataset contains the population evolution of a pest and its biocontrol agent in terms of presence proportion at gall level and absolute number of insects.

The study area extends from the Cuneo region (Piedmont, Italy) to southern Switzerland. In order to provide a complete range of data covering the entire process from the pest arrival to complete biological control by its natural enemy T. sinensis, a space-for-time substitution approach has been adopted so as to create a temporal gradient of the epidemic stages over the whole study area.

The southernmost Swiss sites roughly represent the arrival and establishment of the pest without the presence of the natural enemy, the central ones the early epidemic stage and the epidemic peak, whereas the northern ones the end of the epidemic with the beginning of the biocontrol. The Italian ones represent the beginning of the equilibrium between the two population as well as the situation with stable T. sinensis populations on the long term.

These data are used in the paper entitled: Torymus sinensis local and regional early population dynamics in the Insubrian and Piedmont regions

biological-control

display_name: biological-control

castanea-sativa

display_name: castanea-sativa

dryocosmus-kuriphilus

display_name: dryocosmus-kuriphilus

pest-invasion

display_name: pest-invasion

population-evolution

display_name: population-evolution

southern-switzerland

display_name: southern-switzerland

torymus-sinensis

display_name: torymus-sinensis


en: x-ray-tomography slow-snow-compression-experiments microstructure snow-mechanics ice

en: slow snow compression experiments

media_type: application/zip

en: Compression experiments, X-ray images and with supplemented README file

format: ZIP

en: README

en: README file gives an overview of the organization of the data

en:
We conducted consecutive loading-relaxation experiments at low strain rates to study the viscoplastic behavior of the intact ice matrix in snow. The experiments were conducted using a micro-compression stage within the X-ray tomography scanner in the SLF cold laboratory.

Next, to evaluate the experiments, a novel, implicit solution of a transient scalar model was developed to estimate the stress exponent and time scales in the effective creep relation (Glen's law). The result reveals that, for the first time, a transition in the exponent in Glen's law depends on geometrical grain size. A cross-over of stress exponent $n=1.9$ for fine grains to $n=4.4$ for coarse grains is interpreted as a transition from grain boundary sliding to dislocation creep.

The dataset includes compression force data from 11 experiments and corresponding 3D image data from tomography scans.

ice

display_name: ice

microstructure

display_name: microstructure

slow-snow-compression-experiments

display_name: slow-snow-compression-experiments

snow-mechanics

display_name: snow-mechanics

x-ray-tomography

display_name: x-ray-tomography

en: A grain-size driven transition in the deformation mechanism in slow snow compression


maintainer: Maria Elvira Murazzi

en: forest-trees uptake 13c-labelling polystyrene nanoplastic

en: Nanoplastic in forests experiment

en:
Data embargoed until August 2022, and it will be provided upon request

-  excel file containing: two working sheets (leaf supply and root supply experiment) with a table with the values 13C-nanopolystyrene in the different tissues of the two forest tree species; graph showing the differences between controls and treatments of 13C-nanopolystyrene enrichment for each tissue of each forest tree species; ANOVA table showing significant values after having exposed the tree species to 13C-nanopolystyrene

format: XLS

en:
The fate of plastic in the environment is of global concern, because its production recently has
increased strongly and it accumulates in terrestrial and aquatic ecosystems. Although some knowledge on
its role in aquatic and terrestrial ecosystems was gained in the recent decade, hitherto very little is known
about the impact of micro and nanoplastics in forest ecosystems.

The aim of this pioneering project was to explore if nanoplastics are taken up by forest trees species
through leaves or roots. In greenhouse experiments, we exposed leaves or roots of seedling of two forest
trees species to solutions with highly 13C-labelled polystyrene nanoparticles (13C-nPS, 99 atom%) and
examined if they were incorporated in different above- and belowground tissues.

Treated part of the trees for both species showed significant 13C-enrichment, indicating that trees take up nanoparticles. However, the overall 13C signal strength in tissues that were not exposed to the 13C label remained low (Δδ13C<1‰) and was confined to a few seedlings, leaving it ambiguous whether nanoplastic transport occurs or not.

We acknowledge that the new method developed might be not sensitive enough to unequivocally detect
mechanisms of nanoplastic uptake and transport at environmentally realistic concentrations.

forest-trees

display_name: forest-trees

nanoplastic

display_name: nanoplastic

polystyrene

display_name: polystyrene

uptake

display_name: uptake

educ

en: Education, culture and sport

en: Nanoplastics in forests: Exploring the effects of nanoplastics on forest soils and tree physiology (NanoPlast)


en: preferential-deposition alpine3d tls lidar lee-side-flow-field

en: Dataset

en:
The data presented here corresponds to the publication "A Close-Ridge Small-Scale Atmospheric Flow Field and its Influence on Snow Accumulation" (Gerber et al., 2017), which investigates an eddy-like structure in the vicinity of the Sattelhorn in the Dischma valley (Davos Switzerland) and its influence on snow accumulation. The dataset contains:

* Alpine3D: Alpine3D snow depth grids (25 m resolution) for two simulations with and without snow redistribution.
* ARPS: 10 ARPS simulations (25 m horizontal resolution) with different model setups (wind direction, wind speed, stability).
* LiDAR: Processed LiDAR PPI (D2_PPI_1h) and RHI (D2_cross_1h) across the valley with a hourly resolution for the period 27 October 2015 01:00 - 29 October 2015c 21:00 (spatial resolution: 25 m).
* meteostations-dischma: Meteorological station data of two meteorological stations in the Dischma valley with 10 minute resolution for the period 28 October 2015 - 30 October 2015.
* TLS: Snow depth change data (m) between 28 October 2015 and 30 October 2015 based on terrestrial laser scans.

For more details about the simulation and observation data, see Gerber et al., 2017.

Publication: Gerber et al., 2017: A Close-Ridge Small-Scale Atmospheric Flow Field and its Influence on Snow Accumulation, submitted to JGR - Atmospheres.

lee-side-flow-field

display_name: lee-side-flow-field

lidar

display_name: lidar

preferential-deposition

display_name: preferential-deposition

tls

display_name: tls

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: DISCHMEX - Observations and simulations of the close-ridge small-scale atmospheric flow field and snow accumulation at Sattelhorn, Dischma valley, Davos, Switzerland.


en: urban-ecology place-attachment landscape electrodermal-activity sense-of-place

en: 03. Publication Ready Data

en: 00. EDA Raw Data

en: 04. Scripts

en: 02. Questionnaire Data

en: 05. Visuals

en: 01. EDA Processed Data

en: This repository contains data on EDA measurements of visitors with different cultural backgrounds in virtual urban park settings. The parks are a Persian garden (Shiraz, Iran) and a historical park in Zurich, Switzerland. The cultural background of the visitors is Persian and Central European. The repository contains raw data from EDA, processed time series and statistical procedures.

electrodermal-activity

display_name: electrodermal-activity

landscape

display_name: landscape

place-attachment

display_name: place-attachment

sense-of-place

display_name: sense-of-place

urban-ecology

display_name: urban-ecology

en: Electrodermal Activity (EDA) of Bi-cultural Visitors In Virtual Park Settings


maintainer: Ana Beatriz Pierri Daunt

en: decision-making landscape-services sense-of-place literature-review landscape-ecology socio-ecological-system ecosystem-services planning pdfsearch multifunctionality landscape-planning resilience landscape-perception green-infrastructure

en: 6. Protocol

en: 1. Frequency of early concepts.

en: 4. Use of any additional concept.

en: 5. Planning steps.

en: 3. Use of any early concept.

en: 2. Frequency of additional concepts.

en:
Table of content: 1. Frequency of early concepts; 2. Frequency of additional concepts; 3. Use of any early concept; 4. Use of any additional concept, 5. Planning steps; 6. Protocol.

The present dataset is part of the published scientific paper entitled “Landscape ecological concepts in planning: review of recent developments” (Hersperger et al., 2021). The goal of this research was to review recent publications to assess the use of landscape ecological concepts in planning. Specifically, we address the following research questions: Q1. Landscape ecological concepts: What are they? How frequently are they mentioned in current research? Q2. How are landscape ecological concepts integrated in landscape planning?

We analysed all empirical and overview papers that have been published in four key academic journals in the field of landscape ecology and landscape planning in the years 2015–2019 (n = 1918). Four key journals in the field of landscape ecology were selected to conduct the analysis, respectively Landscape Ecology (LE), Landscape Online (LO), Current Landscape Ecology Reports (CLER), and Landscape and Urban Planning (LUP). The title, abstract and keywords of all papers were read in order to identify landscape ecological concepts. Then, all 1918 papers went through a keyword search to identify the use of early and additional concepts. We used the “pdfsearch” package in R programming language and searched for singular and plural forms and different variations of the concepts (see Supplementary material 1, Table A). As a result, we provided four outputs:

  1. Frequency of early concepts. This data provides the total number of times each article used each early concept (Q1). This data was used to produce the Figure 2a at the original publication.

  2. Frequency of additional concepts. This data provides the total number of times each article used each additional concept (Q1). This data was used to produce the Figure 2b at the original publication.

  3. Use of any early concept. This data provides the total number of times each article used any early concept (Q1).
This data was used to produce the Figure 3a at the original publication.

  4. Use of any additional concept. This data provides the total number of times each article used any additional concept (Q1). This data was used to produce the Figure 3b at the original publication.

To address the second question (Q2), the title, abstract and keywords of the papers included in our sample (n=1918 articles) were screened to identify papers that might show how landscape ecological concepts are integrated into planning. We selected 52 empirical papers (see Supplementary material – 4 Integration of landscape ecological concepts into planning), and we provided two outputs:

  5. Planning steps. This data provides the number of times landscape ecological concepts were addressed in each
planning steps in 52 empirical papers analysed in detail (Q2). This data was used to produce the Figure 4 at the
original publication.

  6. Protocol for assessing the integration of landscape ecological concepts into planning. To systematically collect the
data, we used this protocol which addressed the following questions: (a) which type of planning is addressed by the
paper? (b) to which planning level does the paper refer to? (c) which concepts are integrated in any of the planning
steps described above?

decision-making

display_name: decision-making

ecosystem-services

display_name: ecosystem-services

green-infrastructure

display_name: green-infrastructure

landscape-ecology

display_name: landscape-ecology

landscape-perception

display_name: landscape-perception

landscape-planning

display_name: landscape-planning

landscape-services

display_name: landscape-services

literature-review

display_name: literature-review

multifunctionality

display_name: multifunctionality

pdfsearch

display_name: pdfsearch

planning

display_name: planning

resilience

display_name: resilience

socio-ecological-system

display_name: socio-ecological-system

educ

en: Education, culture and sport

en: The usage of landscape ecological concepts in the planning literature


maintainer: Anita C. Risch

en: grassland native-plant herbivory weed-management non-native-plant

en: Data file native and non-native plant interactions in Australian grasslands

en:
taxa tab:	list and overview of all the recorded species

* family:                 family of the plant

* genus:                genus of the plant

* species:                plant species name

* origin:                indicates whether the plant is native to Australia or not (non-native)

* common name:	        common name of the plant species

* functional group:	        functional group of the plant species: forb, graminoid, fern, tree

* functional group detailed:	gives more detail about the type of graminoid and forb: legume, forb (non-leguminous),
                        grass, sedge, rush, fern, tree

* lifeform:                annual or perennial lifeform (species which can have both of these lifeforms where
                        considered as annuals)

plot tab:	list of species found in each plot and their cover (%)

* sampling.session:        provides information about the sampling time:
                        May2020 = data that was collected in May 2020
                        Nov2020 = data that was collected in November 2020

* farm.name:	            name of the farms where the sampling took place:
                        SF = farm of the Southern group
                        NF = farm of the Northern group
                        numbers 1-3 = randomly allocated to the Southern group of farms
                        numbers 4-6 = randomly allocated to the Northern group of farms

* site.type:	                KG: Kangaroo grass dominated site
                        ALG: ALG+KG site (Kangarooo grass and African lovegrass co-dominated site)

* plot.no:                plot number: 9-12

* scientific.name:	        scientific name of the plant species found in a certain plot

* functional group:	        indicates whether the species found is a tree, forb, graminoid or fern

* functional group detailed:	indicates whether the species found is a tree, legume, forb (non-leguminous),
                        grass, sedge, rush, fern

* lifeform:                specifies the lifeform of the species: annual or perennial lifeform
                        (species which can have both of these lifeforms where considered as annuals)

en:
This dataset contains all data, on which the following publication below is based.

Paper Citation:

_Schlierenzauer, C., Risch, A.C., Schütz, M., Firn, J. 2021. Non-native Eragrostis curvula reduces plant species diversity in pastures of South-eastern Australia even when native Themeda triandra remains co-dominant. Plants 10, 596._

Please cite this paper together with the citation for the datafile.

Study area
The study was conducted in the lowland grassy woodlands of the Bega Valley Region, which is located in the south-east corner of New South Wales, Australia. Embedded between the Pacific Ocean and the Australian Alps, the lowland grassy woodlands are mostly located on granitic substrates and reach elevations of roughly 500 m above sea level. Typically, these grassy woodlands receive less precipitation (mean annual precipitation between 700-1100 mm) compared to the more elevated areas that surround them (NSW Government - Office of Environment and Heritage 2017).
The vegetation is dominated by an open tree canopy layer consisting of Eucalyptus tereticornis Sm, Angophora floribunda Sm. (Sweet) and a range of other eucalypt species. Sometimes shrub or small trees are also present, whereas grasses and forbs form the ground-cover. In areas without intensive agricultural history, this layer is dominated by perennial, tussock grasses such as Themeda triandra Forssk, Microlaena stipoides R.Br (Weeping Grass), Eragrostis leptostachya Steud. (Paddock Lovegrass) and Echinopogon ovatus P.Beauv (Forest Hedgehog Grass). The remaining inter-tussock spaces are occupied by a diversity of growth-restricted grasses and herbaceous forbs (NSW - Department of Planing, Industries and Environment 2019; NSW Government - Office of Environment and Heritage 2017).
Clearing, pasture sowing, fertilizer application and livestock grazing resulted in a dramatic decrease in the extent of these natural woodlands, with less than five percent within conservation reserves and overall, with only about 20% of their original extent in New South Wales still existing (Tozer et al. 2010). The remaining areas outside of reserves are threatened by altered fire frequencies, habitat clearing, livestock grazing and especially by non-native plant invasion, particularly Eragrostis curvula (Schrad.) Nees. For this reason, the grassy woodlands are listed as an endangered ecological community in the NSW state legislation. Additionally, they are considered as critically endangered by the Commonwealth of Australia (Threatened Species Scientific Committee (TSSC) 2013).

Experimental design and sampling
The study was conducted on six farms and in each of them two sites were chosen, representing a paired design. One of the sites at each farm is dominated by native Themeda triandra, the other one co-dominated by non-native Eragrostis curvula and Themeda triandra. All farms are within a radius of approximately 10 km from the town Candelo. Three of the farms are located North (36°40’ to 36°42’ S and 149°38’ to 149°42’ E) and three of them are located South (36°51’ to 36°49’ S and 149°38’ to 149°42’ E) of Candelo.
Non-native herbivores (mainly cattle, sheep and rabbits) and native herbivorous marsupials (mainly kangaroos, wallabies and wombats) are present in the area of these sites. On each site, data was collected within four plots (each 1 x 1 m) in May and November 2020. All plant species found within a plot were recorded and their relative abundance was estimated.

References
NSW - Department of Planing, Industries and Environment. 2019. “Lowland Grassy Woodland in the South East Corner Bioregion - Endangered Ecological Community Listing.” https://www.environment.nsw.gov.au/topics/animals-and-plants/threatened-species/nsw-threatened-species-scientific-committee/determinations/final-determinations/2004-2007/lowland-grassy-woodland-south-east-corner-bioregion-endangered-ecological-community-l (February 18, 2021).
NSW Government - Office of Environment and Heritage. 2017. “Lowland Grassy Woodland in the South East Corner Bioregion - Profile.” https://www.environment.nsw.gov.au/threatenedSpeciesApp/profile.aspx?id=20070 (January 31, 2021).
Threatened Species Scientific Committee (TSSC). 2013. Environment Protection and Biodiversity Conservation Act 1999 (EPBC Act) Conservation Advice for Lowland Grassy Woodland in the South East Corner Bioregion. http://www.environment.gov.au/biodiversity/threatened/communities/pubs/82-conservation-advice.pdf.
Tozer, Mark et al. 2010. “Native Vegetation of Southeast NSW: A Revised Classification and Map for the Coast and Eastern Tablelands.” Cunninghamia : a journal of plant ecology for eastern Australia 11(3): 359–406.

grassland

display_name: grassland

herbivory

display_name: herbivory

native-plant

display_name: native-plant

non-native-plant

display_name: non-native-plant

weed-management

display_name: weed-management

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Native and no-native plant interactions in Australian grasslands


photogrammetric-drone-data-schurlialp

maintainer: Yves Bühler

title_for_slug: photogrammetric-drone-data-schurlialp

en: photogrammetry dsm wingtra orthophoto drone-data uav

en: Data Access

en:
- 10 cm DSM
- 10 cm HS
- 3 cm orthophoto
- original RGB images
- Agisoft Metashape Processing Report

All of the products are also available as a cloud optimized geotiff.

format: SERVICE

en: The data was collected on 16.04.2021 and on 28.05.2021 with a Wingtra Gen II and a Sony RX1 II RGB sensor to obtain snow depth and distribution data. Following the data collection, the data was processed with Agisoft Metashape. A 10cm DSM, a 10cm snow depth raster, a 3mm orthophoto and the original drone images are available for download.

drone-data

display_name: drone-data

dsm

display_name: dsm

orthophoto

display_name: orthophoto

photogrammetry

display_name: photogrammetry

uav

display_name: uav

wingtra

display_name: wingtra

en: Photogrammetric Drone Data Schürlialp


en: swiss-forest-lab meteorology temperature long-term relative-humidity precipitation wind-speed meteo climate-change par

en: Site photo of the field station (NOF)

en: Site photo of the field station from the side.

mimetype: image/jpeg

en: Data plot of the forest station (NOB)

media_type: application/pdf

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

format: PDF

mimetype: application/pdf

en: Site aerial photo (Novaggio)

en: Site aerial photo from both Novaggio stations. Source: Google. 2016. Site aerial photo from both Novaggio stations. Retrieved November 15, 2016, from https://www.google.ch/maps

en: Site photo of the forest station (NOB)

en: Site photo of the forest station from the side.

en: Forest station data (NOB)

en: Data file.

en: Field station data (NOF)

en: Data plot of the field station (NOF)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Novaggio in Switzerland where one station is located within a natural deciduous forest stand (NOB) with Turkey oak (_Quercus cerris_; 70 yrs), sweet chestnut (_Castanea sativa_; 90 yrs) and silver birch (_Betula pendula_; 70 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, NOF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Novaggio is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

en: Novaggio, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1996 onwards


maintainer: Ariel Bergamini

en: biomass bryophytes functional-traits fungicide nitrogen moss pandiv species-richness grassland biodiversity-experiment

en: main data as used in Lin et al. 2025

en:
This dataset was used to analyse effects of different biodiversity and functional group treatments, fertilizer and fungicide applications as well as weeding of vascular plants on the species richness and species composition of bryophytes for the following publication: Lin M., Bergamini A., Pichon N.A., Allan E, Boch S. 2025. Nitrogen enrichment and vascular plant richness loss reduce bryophyte richness. Scientific Reports.
In this study we surveyed 96 of 216 2 m x 2 m experimental plots of the PaNDiv experiment, a large grassland field experiment in the Swiss lowlands (Pichon et al. 2020). In the PaNDiv experiment four experimental treatments were applied in a full-factorial design manipulating: (1) the nitrogen availability (through fertilization), (2) the presence of fungal pathogens (through fungicide application), (3) the functional composition of the vascular plant community (represented here by community-weighted mean (CWM) SLA), and (4) the vascular plant richness (sowing of either 1, 4, 8 or 20 species). To maintain the original sown vascular plant species richness and composition, one part of each plot (1.5 m × 2 m) was weeded, while the other part has been left unweeded since the beginning of the experiment (0.5 m × 2 m).
On each plot we surveyed two subplots, the unweeded subplot and a weeded subplot of the same area (0.5 m × 2 m). On each subplot we established a complete list of all bryophyte species together with an estimate of their cover.
The provided excel file contains three sheets:
1.	A sheet with raw species lists of all plots surveyed together with information on all treatments and an estimation of the percentage cover of vascular plants in a summer campaign in August 2022. Cover of bryophytes was estimated for each species in percent.
2.	A sheet with a list of all bryophyte species with its growth form (acroarpous/pleurocarpous)
3.	A sheet with some explantations
By means of the provided R code and the data in the excel file, all analyses can be repeated.
More details to the data sampled can be found in Lin et al (2025).

biodiversity-experiment

display_name: biodiversity-experiment

biomass

display_name: biomass

bryophytes

display_name: bryophytes

functional-traits

display_name: functional-traits

fungicide

display_name: fungicide

grassland

display_name: grassland

moss

display_name: moss

nitrogen

display_name: nitrogen

pandiv

display_name: pandiv

species-richness

display_name: species-richness

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Data on bryophyte diversity and treatment factors in the PaNDiv experiment


en: land-use-change digital-zoning-plans spatial-planning land-use-simulations land-use-transformations

en: Legend simulated maps

en:
Table of Content: 1. General context of the data set "LUSzoning”; 2. Background and aims of the study using the data set LUSzoning; 3. The data set LUSzoning.
1. General context of the data set "LUSzoning".
The data set "LUSzoning" stands for Land-use simulations integrating zoning regulations in Spanish functional urban areas. The data set has been generated as part of the CONCUR research project (https://www.wsl.ch/en/projects/concur.html) led by Dr. Anna M. Hersperger and funded by the Swiss National Science Foundation (ERC TBS Consolidator Grant (ID: BSCGIO 157789) for the period 2016-2021. The CONCUR research project is interdisciplinary and aims to develop a scientific basis for adequately integrating spatial policies (in this case, digital zoning plans) into quantitative land-change modelling approaches at the urban regional level.
2.	Background and aims of the study using the data set “LUSzoning”.
As part of the CONCUR project, a specific task was to integrate planning spatial policies in land-change modelling. Planning can be implemented in modelling using either hard or gradual restrictions. Different studies have addressed the inclusion of spatial planning policies in land-use change modelling. However, the integration of zoning constraints is generally established as hard or Boolean-based restrictions (e.g., whether urban development is allowed or not), while not accounting for the spatial heterogeneity or gradual characteristics within planning zones (e.g., whether planning regulations allow low, medium or high urban density), though these could improve real patterns simulations in urban areas. We assume Spanish General Zoning plans were suitable to explore the integration of planning into land-change modelling as soft constrains because they define land-use intensities in the buildable zoning areas. In light of the above considerations, the overall aim of the study was to model urban land-use changes using a multi-scenario approach that integrates digitized zoning plans for the Functional Urban Areas (FUAs) of Madrid, Barcelona, Valencia, and Zaragoza. The following specific objectives were addressed: i) to analyse the role of planning by defining three future scenarios that integrate digitized zoning plans and one scenario that assumes almost no planning intervention; ii) to introduce zoning constraints that reflect different degrees of urban densities; iii) to generate a transferable spatially-explicit modelling framework to integrate planning into land-use change simulations.
Four future land-use demands scenarios were defined for the FUAs. Storylines were created considering probable development scenarios related to zoning plans, current Spanish legislation and sustainability goals defined along two axes: a high market-oriented vs. high planning-intervention axis, and an axis of short-term economic growth vs. long-term sustainable growth. The sustainable development scenario (S1) is characterized by low gross floor area (GFA) growth that is limited to areas that are currently under development according to zoning plans. The business-as-usual scenario (S2) is characterized by medium GFA growth in the range of on-going trends. The strong development scenario (S3) is characterized by high GFA growth rates. Growth is restricted to buildable areas without urbanization project designated in zoning plans. The unrestricted development scenario (S4) prioritizes a high degree of market liberalization characterized by high GFA growth that surpasses population demands. S4 follows a rapid economic growth pattern with almost no planning intervention.
3.	 The data set “LUSzoning”.
The dataset includes 16 .asc raster layers providing the simulated land-uses under four defined scenarios for Barcelona, Madrid, Valencia and Zaragoza Functional Urban Areas (FUAs) for 2030. The simulated raster layers were created using CLUMondo simulation framework and have a spatial resolution of 30m. The .asc layers name include the name of the FUA and scenario number. For example, the output from simulating the urban growth for the city of Zaragoza under Scenario 2 is named “Zaragoza_S2.tif”. Furthermore, a .txt file named “Legend.txt” includes the numeric value of the land-use and the category of land-use that represents to interpret the .asc raster layers. The name of the land-use classes is a reclassification of the Urban Atlas 2012 land-use classes within the four Spanish FUAs analyzed.

digital-zoning-plans

display_name: digital-zoning-plans

land-use-change

display_name: land-use-change

land-use-simulations

display_name: land-use-simulations

land-use-transformations

display_name: land-use-transformations

spatial-planning

display_name: spatial-planning

en: LUSzoning: Land-use simulations integrating zoning regulations in Spanish functional urban areas


en: switzerland edna insect minion drone ins16s

en: Data access

en: Please use cyberduck to download

en:
Traditional methods of biodiversity monitoring are often logistically challenging, time-consuming, require experienced experts on species identification and sometimes include destruction of the targeted specimens. Here, we investigated a non-invasive approach of combining the use of drones and environmental DNA (eDNA) to monitor insect biodiversity on vegetation.
We aimed to assess the efficiency of this novel method in capturing insect diversity and comparing insect composition across different vegetation types (grassland, shrub, and forest) in Switzerland. A commercial, off-the-shelf drone was equipped with a specialised probe that autonomously swabbed vegetation and collected eDNA. Then, samples were processed using rapid third-generation Oxford Nanopore sequencing. The obtained data were analysed for insect diversity, comparing taxonomic richness, evenness and community composition across the three habitat types using statistical techniques. Sequencing of the samples yielded 76 hexapods taxa, revealing an insect community with notable differences in taxonomic richness but not in evenness across grassland, shrub, and forest habitats.
Our study demonstrates the potential of drone-based sampling integrated with eDNA and nanopore sequencing for biodiversity monitoring, offering a non-destructive method for detecting insect occurrence on plant surfaces. Integrating robotics and eDNA technology provides a promising solution for fast, large-scale, non-invasive biodiversity monitoring, potentially improving conservation efforts and ecosystem management.

drone

display_name: drone

edna

display_name: edna

insect

display_name: insect

minion

display_name: minion

switzerland

display_name: switzerland

en: Robot-aided measurement of insect diversity on vegetation using environmental DNA


disdrometer-data-davos-wolfgang

title_for_slug: disdrometer-data-davos-wolfgang

en: fall-velocity precipitation raclets davos disdrometer

en: Disdrometer Data Davos Wolfgang

en:
The zip folder contains one netCDF file per day for Davos Wolfgang from February 8 to March 19 2019 with a temporal resolution of 30 seconds (time in UTC) and includes:

- characteristics of hydrometeors (e.g. diameter, fall velocity, amount per diameter class,...)
- precipitation rate
- radar reflectivity
- visibility range
- weather codes
- instrument performance

mimetype: application/zip

en: The dataset contains information on precipitation amount and type for Davos Wolfgang (LON: 9.853594, LAT: 46.835577) from February 8 to March 19 2019. It includes: characteristics of hydrometeors (e.g. diameter, fall velocity, amount per diameter class,...), precipitation rate, radar reflectivity, visibility range, weather codes and instrument performance.

davos

display_name: davos

disdrometer

display_name: disdrometer

fall-velocity

display_name: fall-velocity

precipitation

display_name: precipitation

raclets

display_name: raclets


en: functional-taxonomic-groups biodiversity functional-taxonomic-group photobiont moss lichen-growth-form elevational-gradient bryophyte-life-strategy climate-change lichen-growthform climate-change-impact bryophyte liverwort madeira-island endemic disturbance lichen endemic-species

en: MadCrypto – Bryophyte and macrolichen diversity in laurel forests of Madeira

en:
This dataset includes species lists of bryophytes and macrolichens (presence/absence) sampled on the forest floor and on trees in disturbed and undisturbed plots along elevation gradients in the laurel forests of Madeira island. It also contains species specific information (bryophytes: red list status, endemic status, taxonomic group, life strategy; macrolichens: photobiont type, growth form) as well as plot information (Plot_ID, sampling date, coordinates, elevation a.s.l. (m), disturbance type, sampled host tree species).

The dataset was used for the paper
Boch S, Martins A, Ruas S, Fontinha S, Carvalho P, Reis F, Bergamini A, Sim-Sim M (2019) Bryophyte and macrolichen diversity show contrasting elevation relationships and are negatively affected by disturbances in laurel forests of Madeira island. Journal of Vegetation Science.

en:
This dataset includes species lists of bryophytes and macrolichens (presence/absence) sampled on the forest floor and on trees in disturbed and undisturbed plots along elevation gradients in the laurel forests of Madeira island. It also contains species specific information (bryophytes: red list status, endemic status, taxonomic group, life strategy; macrolichens: photobiont type, growth form) as well as plot information (Plot_ID, sampling date, coordinates, elevation a.s.l. (m), disturbance type, sampled host tree species).

The dataset was used for the paper
Boch S, Martins A, Ruas S, Fontinha S, Carvalho P, Reis F, Bergamini A, Sim-Sim M (2019) Bryophyte and macrolichen diversity show contrasting elevation relationships and are negatively affected by disturbances in laurel forests of Madeira island. Journal of Vegetation Science 30: 1122–1133.

The excel file contains 5 sheets:
1) Plot information
2) Bryophyte data with species specific information, separated per substrate
3) Macrolichen data with species specific information, separated per substrate
4) Bryophyte data with species specific information (plot level data). Species lists of the two substrates were merged; if one substrate in a plot hosted a species identified only to genus level (genus spec.) but the second investigated substrate hosted an identified species of the same genus, we removed the genus spec. entry in the particular plot. The species list of sheet 2 and 4 might therefore differ slightly.
5) Macrolichen data with species specific information (plot level data). Species lists of the two substrates were merged; if one substrate in a plot hosted a species identified only to genus level (genus spec.) but the second investigated substrate hosted an identified species of the same genus, we removed the genus spec. entry in the particular plot. The species list of sheet 3 and 5 might therefore differ slightly.

biodiversity

display_name: biodiversity

bryophyte

display_name: bryophyte

bryophyte-life-strategy

display_name: bryophyte-life-strategy

climate-change

display_name: climate-change

climate-change-impact

display_name: climate-change-impact

disturbance

display_name: disturbance

elevational-gradient

display_name: elevational-gradient

endemic

display_name: endemic

endemic-species

display_name: endemic-species

functional-taxonomic-group

display_name: functional-taxonomic-group

functional-taxonomic-groups

display_name: functional-taxonomic-groups

lichen

display_name: lichen

lichen-growth-form

display_name: lichen-growth-form

lichen-growthform

display_name: lichen-growthform

liverwort

display_name: liverwort

madeira-island

display_name: madeira-island

moss

display_name: moss

photobiont

display_name: photobiont

educ

en: Education, culture and sport


maintainer: Matthias Haeni

en: precipitation par climate-change long-term temperature meteo swiss-forest-lab meteorology relative-humidity wind-speed

en: Site aerial photo (Jussy)

en: Site aerial photo from both Jussy stations. Source: Google. 2016. Site aerial photo from both Jussy stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Data plot of the field station (JUF)

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

en: Site photo of the field station (JUF)

en: Site photo of the field station from the side.

en: Site photo of the forest station (JUB)

en: Site photo of the forest station from the side.

en: Data file.

en: Forest station data (JUB)

en: Data plot of the forest station (JUB)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Jussy in Switzerland where one station is located within a natural broad-leaved forest stand (JUB) with sessile oak (_Quercus petrea_; 90 yrs), aspen (_Populus tremula_; 60 yrs) and European hornbeam (_Carpinus betulus_; 60 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, JUF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Jussy is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Jussy, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


maintainer: Joan Casanelles Abella

en: tartu poznan cavity-nesting-bees antwerp paris osmia-cornuta zurich trap-nest osmia-bicornis hylaeus-communis plant-pollinator-interactions dna-metabarcoding urban-ecology chelostoma-florisomne

en: Information on the study sites

en: For each site, IDs, city, coordinates, structure where trapnest was attahced and height of the trapnest is provided

en: Larval food composition

en: Explanation data variables

en: Urbanization poses threats and opportunities for the biodiversity of wild bees. A main gap relates to the food preferences of wild bees in urban ecosystems, which usually harbour large numbers of plant species, particularly at the larval stage. This data sets describes the larval food of four wild bee species (i.e. Chelostoma florisomne, Hylaeus communis, Osmica bicornis and Osmia cornuta) and three genera (i.e. Chelostoma sp., Hylaeus sp, and Osmia sp.) common in urban areas in five different European cities (i.e. Antwerp, Paris, Poznan, Tartu and Zurich). This data results from a European-level study aimed at understanding the effects of urbanization on biodiversity across different cities and citiscapes, and a Swiss project aimed at understanding the effects of urban ecosystems in wild bee feeding behaviour. Wild bees were sampled using standardized trap-nests in 80 sites (32 in Zurich and 12 in each of the remaining cities), selected following a double gradient of available habitat at local and landscape scales. Larval pollen was obtained from the bee nests and identified using DNA metabarconding. The data provides the plant composition at the species or genus level of the different bee nests of the studied species in the studied sites of the five European cities. For Hylaeus communis, this is the first study in reporting larval food composition.

antwerp

display_name: antwerp

cavity-nesting-bees

display_name: cavity-nesting-bees

chelostoma-florisomne

display_name: chelostoma-florisomne

dna-metabarcoding

display_name: dna-metabarcoding

hylaeus-communis

display_name: hylaeus-communis

osmia-bicornis

display_name: osmia-bicornis

osmia-cornuta

display_name: osmia-cornuta

paris

display_name: paris

plant-pollinator-interactions

display_name: plant-pollinator-interactions

poznan

display_name: poznan

tartu

display_name: tartu

trap-nest

display_name: trap-nest

urban-ecology

display_name: urban-ecology

zurich

display_name: zurich

educ

en: Education, culture and sport

en: Larval food composition of four wild bee species in five European cities


maintainer: Camille Albouy

en: atlantic-ocean barcoding teleo-primers 12s mitochondrial-dna actinopterygii environmental-dna

en: This file contains the 12s mitochondrial sequences for the Atlantic fishes collected on a French scientific survey (Evhoe) for stock assessment.

en: This file contains the metadata associated with each scientific survey

en: This file contains the Teleo Barcode for the Atlantic fishes collected during a French scientific survey (Evhoe) for stock assessment.

en: This file contains the metadata associated with each 12 s mitochondrial sequence.

en:
Mitochondrial tree based on the 12s sequences obtained for altantic fish.
This data represent the maximum likelihood phylogenetic tree allowing to assess 1) the clustering of the different sequences generated in
this study and their closest sequences recovered through a MegaBLAST on GenBank, 2) the robustness of the clades and 3) the
phylogenetic dissimilarities through the branch length of the tree among the different sequences. This tree was built with IQtree2
using the best substitution model found by modelFinder. The robustness of the node was evaluated using 1000 ultra-fast bootstrap
(node information indicated on the right of the /) and SH-aLRT procedures (node information indicated on the left of the /), using a hillclimbing nearest neighbor interchange (NNI) search to reduce the risk of overestimating branch supports with ultra-fast Bootstrap
caused by too severe model violations.

en:
The global biodiversity crisis driven by anthropogenic pressures significantly threatens marine ecosystems functioning. The rate of climate change and the impacts of anthropogenic pressures outpacing the capabilities of our observation tools, stresses the need to develop new methods to assess these rapid modifications. Environmental DNA (eDNA; DNA traces released by organisms) metabarcoding has emerged as a non-invasive method that has been widely developed over the last decade. Thanks to a large spatio-temporal coverage, high detection of rare species and its time and cost effectiveness, eDNA metabarcoding represents a promising biomonitoring tool. However, capturing fish diversity using eDNA requires a high-quality genetic reference database, which we are currently still lacking. For the South European Atlantic shelf area, we estimated that only 41% of the fish species present were recorded in the available eDNA reference databases. Improving reference databases can notably rely on opportunistic sampling enabling the reporting of sequences for new species. Therefore, the data provided here consists of barcoding 95 species of ray-finned and cartilaginous fishes over the 12S mitochondrial DNA gene. We generated 168 12S barcodes from fishes that were sampled in the Bay of Biscay (Northeast Atlantic, France) between 2017 and 2019. We also provided the “Teleo” barcode associated with a specific 12S region for each individual. In addition to the sequences, we provided for each individual the taxonomy, the details associated with the barcode (Genbank accession number, chromatograms), a photograph, as well as 5 ecomorphological measures and 11 life-history traits. These traits document several functions such as dispersion, diet, habitat use, and position in the food web. Furthermore, we provided the metadata of each sampling site (date, station, sampling hour, gear, latitude, longitude, depth) and environmental variables measured in situ (conductivity, salinity, water temperature, water density, air temperature).
This data set is highly valuable to improve the Northeast Atlantic eDNA genetic database, thus helping to better understand the effects of environmental forcing in the Bay of Biscay, a transition zone housing mixed assemblages of boreal, temperate and subtropical fish species susceptible to display variability in functional traits to adapt to changing conditions.

actinopterygii

display_name: actinopterygii

atlantic-ocean

display_name: atlantic-ocean

barcoding

display_name: barcoding

environmental-dna

display_name: environmental-dna

mitochondrial-dna

display_name: mitochondrial-dna

teleo-primers

display_name: teleo-primers

en: ATLFISHREF A 12S mitochondrial reference dataset for metabarcoding Atlantic Fishes frequently caught during scientific surveys in the Bay of Biscay


maintainer: Gesa von Hirschheydt

en: monitoring lichens swisslichens detection-probability bayesian-inference observer-error detectability occupancy-model biodiversity inter-observer-difference cryptogams sessile-species

en: Repository containing the full data, code, README and workflow files.

en:
The available lichen data consists of detection/nondetection data (1/0) of 373 tree-inhabiting (*corticolous*) lichen species from 416 plots surveyed 1-2 times. The lichen data were originally collected for the purpose of the Red List of epiphytic lichen species in Switzerland ([Scheidegger *et al.* 2002](https://www.bafu.admin.ch/bafu/de/home/themen/biodiversitaet/publikationen-studien/publikationen/rote-liste-gefaehrdete-arten-baum-erdbewohnende-flechten.html)), but updated to recent nomenclature for the purpose of this study.
This repository contains all the supporting data and R code for the paper:
von Hirschheydt, G., Kéry, M., Ekman, S., Stofer, S., Dietrich, M., Keller, C., Scheidegger, C. (2024) **Occupancy model reveals limited detectability of lichens in a standardised large-scale monitoring**. *Journal of Vegetation Science*.
Results and figures presented in the manuscript should be reproducible (with small differences in the latter digits due to stochasticity of the MCMC sampler) with the provided data and code.
The downloadable `.zip` folder has the following structure:
* `0_data/`
* `1_code/`
* `2_output/`
* `lichen_detectability.Rproj`
* `README.txt`
* `workflow.html`
- `workflow.Rmd`

The main folder and the three subordinate folders each have their own `README*.txt` file. These describe each available file in detail and should be consulted prior to using the data or running any code. The file `lichen_detectability.Rproj` stores the information about the R project. The user can open the project by clicking/double-clicking on this file which will automatically define the repository as working directory for the R session. If the user does not use RStudio/Posit, they may have to set the working directory manually to the stored location in the R files. The files `workflow.*` guide the user through the analysis (`1_code/*.R`) in the correct order so that they can:
- bundle the cleaned data into a data list readable for JAGS
- fit the multi-species occupancy model to the data and store the output
- assess the goodness-of-fit of the model to the data
- conduct a prior sensitivity analysis with 2 additional sets of priors
- extract the summary statistics reported in the manuscript and supplementary materials
- generate the figures shown in the manuscript and supplementary materials

bayesian-inference

display_name: bayesian-inference

biodiversity

display_name: biodiversity

cryptogams

display_name: cryptogams

detectability

display_name: detectability

detection-probability

display_name: detection-probability

inter-observer-difference

display_name: inter-observer-difference

lichens

display_name: lichens

monitoring

display_name: monitoring

observer-error

display_name: observer-error

occupancy-model

display_name: occupancy-model

sessile-species

display_name: sessile-species

swisslichens

display_name: swisslichens

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Repeated detection-nondetection data of corticolous lichens from a standardised monitoring across Switzerland


historical-vegetation-height-model-nfi

maintainer: Mauro Marty

title_for_slug: historical-vegetation-height-model-nfi

en: nfi dsm historical forest vhm photogrammetry

en: 1990-1998 Vegetation Height Model

format: GeoTIFF

en: 1998-2006 Vegetation Height Model

en: 1979-1985 Vegetation Height Model

en: 1985-1991 Vegetation Height Model

en: Description of available data

en:
The main datasets available are 4 nationwide **Digital Surface Models (DSMs)** and corresponding **Vegetation Height Models (VHMs)** with a spatial resolution of 1 m.
The Vegetation Height Models are calculated by subtraction of a Digital Terrain Model (swissAlti3d version 2017, swisstopo) from the generated DSMs. DSMs were derived by image matching of **scanned historical images** (panchromatic 8-bit) acquired with a RC10 frame camera, in the context of the swisswide image acquisition campaigns of swisstopo.
Nationwide products could be generated for the **four epochs**
 1979-1985 / 1985-1991 / 1990-1998 / 1998-2006.
Additional information about successful image matching vs. interpolation of pixels and about the year of image acquisition are available in the corresponding files (**matched_area_xxxx_xxxx_binay.tif** and **meta_acquisition_year_xxxx_xxxx.json**, respectively).
To separate vegetation from non-vegetation areas a mask derived from a combination of information of the Topographic landscape model - TLM (swisstopo) and a NDVI layer was used and is supplied as **"TLM_mask.tif"**
Due to the occurrence of unrealistic vegetation height values caused by the failure of the image matching process (mainly overexposed images and clouds or very steep rocky terrain), pixel values > 60 m were set to NoData and < 0 m to 0.
All datasets are available in the Reference System **LV95 (EPSG: 2056) LN02 (EPSG: 5728)** and share the common **extent definition** (xmin ymin xmax ymax): 2480000 1070000 2840000 1300000

dsm

display_name: dsm

forest

display_name: forest

historical

display_name: historical

nfi

display_name: nfi

photogrammetry

display_name: photogrammetry

vhm

display_name: vhm

en: Historical Vegetation Height Model NFI