en: lad-use-chages bees urban dietary-patterns foraging-behaviour macronutrients pollen-metabarcoding nutrient-intake bombus-pasquorum rural zurich bombus-lapidarius basel bern bumblebees

en:
Processed data. The dataset contains the functional diversity metrics based on the plant composition from the leg-baskets per bumblebee individual. It contains:
- SampleID: Combining bumblebee ID, location, landscape and replica
- Location: canton, levels: Bern =BE; Basel = BS; Zurich =ZH
- Landscape: landscape type, levels: Urban = U; Rural = R
- Replicate: sampling place (800-m buffer) within location and landscape. Levels: A-E.
- LocationLandscape: combining location and landscape
- Bumblebee_species: the two studied species, levels: B.pasquorum; B. lapidarius
- Fric.w: Weighted functional richness
-Fric: functional richness
-FEve.w: Weighted functional evenness
- FEve: functional evenness
- FDiv.w: weighted functional divergence
- FDiv: functional divergence

en:
The dataset contains the information on the 16 study sites:
- Location: the three Swiss cantons studied. Levels: Bern =BE; Basel = BS; Zurich =ZH
- Landscape: the two typea of human-dominated ecosystems, nested to the Swiss canton. Levels: Urban = U; Rural = R
- Replicate: sampling place (800-m buffer) nested with the location and the landscape. Levels: A-E.
- Site_ID: combination of location, landscape and replicate
- X: X coordinate, WGS84
- Y: Y coordinate WGS84
- Plant S in landscape: Plant species richness within the 800 m calculated using both information from the Global Biodiversity Information. Facility (GBIF) and InfoFlora (www.infoflora.ch).
- % Buildings: the proportion of buildings and other impervious surfaces within 800 m radius.
- % Agricultural: the proportion of agricultural land and other impervious surfaces within 800 m radius.
- % Water: the proportion of water and other impervious surfaces within 800 m radius.
- % Meadows & grasslands: the proportion of meadows and grasslands and other impervious surfaces within 800 m radius.
The raw metabarcoding data is archived in NCBI project PRJNA1137320.

en:
The dataset contains the filtered plant composition1 (removing species with too low relative abundances) and the relative abundance obtained from DNA metabarcoding on pollen samples for both the leg-baskets and the body. The dataset contains the following columns:
- SampleID: Combining bumblebee ID, location, landscape and replica
- Abundance: numeric, relative abundance
- BumblebeeID: numerical ID for each bumblebee individual within a location, landscape and replicate sampling place.
- Location: canton, levels: Bern =BE; Basel = BS; Zurich =ZH
- Landscape: landscape type, levels: Urban = U; Rural = R
- Replicate: sampling place (800-m buffer) within location and landscape. Levels: A-E.
- LocationLandscape:
- Bumblebee_species: the two studied species, levels: B.pasquorum; B. lapidarius
- Transportation_structure: bumblebee pollen transportation structure, levels: B = Body; L = leg-baskets (i.e. corbicula)
- Kingddom: taxonomic
- Phylum: taxonomic
- Order: taxonomic
- Family: taxonomic
- Genus: taxonomic
- Species: plant sicentific name
-Binomial_abundance: Logic, indicating if the plant species is included (1).
The raw metabarcoding data is archived in NCBI project PRJNA1137320

en:
The dataset contains the amino acid content and ratios for the two bumblebees. Measures are taken only from pollen from the leg-baskets. SampleID is not the same as in the metabarcoding, several samples from different bumblebees were merged within a Location ,Landscape and Replica. Not for all the samples there is information on both amino acids and fatty acids. The columns are:
- SampleID_chemical: The ID for chemical analyses
- Location: the three Swiss cantons studied. Levels: Bern =BE; Basel = BS; Zurich =ZH
- Landscape: the two typea of human-dominated ecosystems, nested to the Swiss canton. Levels: Urban = U; Rural = R
- Replicate: sampling place (800-m buffer) nested with the location and the landscape. Levels: A-E.
- Bumblebee_species: the two studied species, levels: B.pasquorum; B. lapidarius
- LocationLandscape: Combining location and landscape
- Asp, Thr, ..., Arg: Content of individual amino acids. Amino acids are written as abbreviated names. FUll names be checked here: https://en.wikipedia.org/wiki/Amino_acid
- sum: total sum of amino acid content (mg)
- sumessential: total sum essential amino acids (mg)
-sumnon-essential: total sum non-essential amino acids (mg)
- proportionnonessentialessential: proportion between essential and non-essential amino acids.

en:
The dataset contains the plant trait values obtined from published sources. It contains:
- Kingddom: taxonomic
- Phylum: taxonomic
- Order: taxonomic
- Family: taxonomic
- Genus: taxonomic
- Species: species name
- plant.species: full scientific name
- native_exotic: origin status. As presented in https://www.doi.org/10.16904/envidat.210
- pollination mode: vector of pollination. As presented in https://www.doi.org/10.16904/envidat.210
- Flowering_months_duration: Number of flowering months. As presented in https://www.doi.org/10.16904/envidat.210
- start_flowering: month of the starting of the flowering. As presented in https://www.doi.org/10.16904/envidat.210
- growth_form_category: plant growth form. As presented in https://www.doi.org/10.16904/envidat.210
- plant-height: plant heght in meters. As presented in https://www.doi.org/10.16904/envidat.210
- inflorescence: Logic, plant with single flowers (0) or inflorescences (1). As presented in https://www.doi.org/10.16904/envidat.210
- structural_blossom_class: depicting the accessibility of the flowers. As presented in https://www.doi.org/10.16904/envidat.210
- symmetry: floral symmetry. As presented in https://www.doi.org/10.16904/envidat.210
- nectar: logic, nectar present. As presented in https://www.doi.org/10.16904/envidat.210
- pollen: logic, pollen rewards present. As presented in https://www.doi.org/10.16904/envidat.210
- oil: logic. oil present. As presented in https://www.doi.org/10.16904/envidat.210
- sugar.concentration: the amount of sugar present in the nectar, in mg.
Additional information can be found in https://doi.org/10.1016/j.dib.2021.107243

en:
The dataset contains the fatty acid content and ratios for the two bumblebees. Measures are taken only from pollen from the leg-baskets. SampleID is not the same as in the metabarcoding, several samples from different bumblebees were merged within a Location ,Landscape and Replica. Not for all the samples there is information on both amino acids and fatty acids. The columns are:
- SampleID_chemical: The ID for chemical analyses
- Location: the three Swiss cantons studied. Levels: Bern =BE; Basel = BS; Zurich =ZH
- Landscape: the two typea of human-dominated ecosystems, nested to the Swiss canton. Levels: Urban = U; Rural = R
- Replicate: sampling place (800-m buffer) nested with the location and the landscape. Levels: A-E.
- Bumblebee_species: the two studied species, levels: B.pasquorum; B. lapidarius
- LocationLandscape: combination between location and landscape
- Caprylic, Pelargonic, ..., Lacceroic: Content of individual fatty acids acids.
- sum: total sum of fatty acid content (mg)
- unsaturatedsum: total sum unsaturated fatty acids (mg)
- saturatedsum: total sum saturated fatty acids (mg)
- omega3sum: total sum Omega 3 fatty acids (mg)
- omega6sum: total sum Omega 6 fatty acids (mg)
- omega9sum: total sum Omega 9 fatty acids (mg)
- omega6to3ratio: ratio Omega 6 to Omega3 fatty acids
-saturatedtounsaturatedration: ratio between saturated and unsaturated fatty acids

en:
Processed data. The dataset contains the phylogeneitc diversity metrics based on the plant composition from the leg-baskets per bumblebee individual. It contains:
- SampleID: Combining bumblebee ID, location, landscape and replica
- Location: canton, levels: Bern =BE; Basel = BS; Zurich =ZH
- Landscape: landscape type, levels: Urban = U; Rural = R
- Replicate: sampling place (800-m buffer) within location and landscape. Levels: A-E.
- LocationLandscape: combining location and landscape
- Bumblebee_species: the two studied species, levels: B.pasquorum; B. lapidarius
- PVar: phylogenetic variance
- PRic: Phylogenetic richness
- PEve: Phylogenetic evenness
- PClu: Phylogenetic clustering

en:
Processed data. The dataset contains the plant species richness in the leg-baskets per bumblebee individual. It contains:
- SampleID: Combining bumblebee ID, location, landscape and replica
- Location: canton, levels: Bern =BE; Basel = BS; Zurich =ZH
- Landscape: landscape type, levels: Urban = U; Rural = R
- Replicate: sampling place (800-m buffer) within location and landscape. Levels: A-E.
- LocationLandscape: combining location and landscape
- Bumblebee_species: the two studied species, levels: B.pasquorum; B. lapidarius
- Plant species richness: the number of plant species found in the pollen of the leg-baskets.

en:
Data on the diet of urban and rural bumblebees individuals, Bombus lapidarius and Bombus pascuorum, collected in Switzerland (cantons Bern, Basel and Zürich) obtained using metabarconding and chemical analyses from two pollen transportation structures, that is, the body and the leg-baskets (i.e., corbicula). The bumblebees were collected by Egenberger et al., 2019 (https://doi.org/10.5061/dryad.qt8mr4v)
The data contains:
- The composition and relartive abundance of plant species in the body and the leg-baskets, separately.
- The content and ratios of two groups of macronutrients, that is, amino acids and fatty acids.
The dataset contains the data used in the paper "Urban bumblebees diversify their foraging strategy to maintain nutrient intake" as well as unpublished data.

basel

display_name: basel

bees

display_name: bees

bern

display_name: bern

bombus-lapidarius

display_name: bombus-lapidarius

bombus-pasquorum

display_name: bombus-pasquorum

bumblebees

display_name: bumblebees

dietary-patterns

display_name: dietary-patterns

foraging-behaviour

display_name: foraging-behaviour

lad-use-chages

display_name: lad-use-chages

macronutrients

display_name: macronutrients

nutrient-intake

display_name: nutrient-intake

pollen-metabarcoding

display_name: pollen-metabarcoding

rural

display_name: rural

urban

display_name: urban

zurich

display_name: zurich


data-analysis-toolkits

maintainer: EnviDat Support

title_for_slug: data-analysis-toolkits

en: hypothesis-testing uncertainty bayesian-inference data-analysis statistics error-propagation

state: active

type: dataset

en: Toolkit 10: linear regression

media_type: application/pdf

en: An overview of the math, logic, and pitfalls of ordinary least-squares linear regression

format: PDF

mimetype: application/pdf

en: Toolkit 12: weighted averages and their uncertainties

en: There are two different ways to calculate the uncertainty of a weighted average. Software packages often don't tell you which one they are reporting, and the resulting errors can be huge.

en: Toolkit 3: tools for transforming data

en: The ladder of powers and a few other cool transformations

en: Toolkit 11: serial correlation

en: Tools for detecting and correcting for serial correlation in analyses of time series

en: Toolkit 2: quantifying data distributions using descriptive statistics

en: Toolkit 1: graphically displaying data distributions

en: toolkit 6: averaging in nonlinear functions

en: The average of a nonlinear function will not equal the function evaluated at its average inputs (the mean of the function will differ from from the function of the mean). This toolkit shows how to estimate that averaging bias.

en: Toolkit 9: Experimentwise error rates and statistical fishing expeditions

en: Statistical fishing expeditions, and how to avoid being misled by them

format: SERVICE

en: Toolkit 4: confidence intervals

en: Toolkit 7: hypothesis testing, significance, and power

en: An overview of basic concepts -- and common pitfalls -- in statistical hypothesis testing, statistical significance, and power analysis

en: Toolkit 8: Hodges-Lehmann estimators

en: A quick introduction to Hodges-Lehmann estimators (nonparametric measures of effect size)

en: Toolkit 5: uncertainty analysis and error propagation

en: Gaussian error propagation, monte carlo methods, and first-order, second-moment error propagation

en:
These are condensed notes covering selected key points in data analysis and statistics. They were developed by James Kirchner for the course "Analysis of Environmental Data" at Berkeley in the 1990's and 2000's. They are not intended to be comprehensive, and thus are not a substitute for a good textbook or a good education!

License: These notes are released by James Kirchner under a Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) license.

bayesian-inference

display_name: bayesian-inference

data-analysis

display_name: data-analysis

error-propagation

display_name: error-propagation

hypothesis-testing

display_name: hypothesis-testing

statistics

display_name: statistics

uncertainty

display_name: uncertainty

educ

fr: Éducation, culture et sport

de: Bildung, Kultur und Sport

en: Education, culture and sport

it: Istruzione, cultura e sport

envidat

fr: EnviDat: le portail de données environnementales

de: EnviDat: das Umwelt-Datenportal

en: EnviDat: the Environmental Data Portal

it: EnviDat: il portale dei dati ambientali

fr: EnviDat est le portail de données du WSL; il offre un accès uniformisé et réglementé aux données de surveillance et de recherche en matière d’environnement. Ce portail est capable d’héberger et de publier des ensembles de données précieuses et uniques, qui sont partagées aux quatre coins de la planète. Ce faisant, EnviDat favorise la recherche et contribue à l’avancement des sciences environnementales.

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

de: EnviDat ist das Datenportal der WSL für einen einheitlichen und verwalteten Zugang zu Umweltdaten aus Monitoring- und Forschungsprojekten. Auf dem Portal können Datensätze gehostet und veröffentlicht werden. Durch die weltweite Bereitstellung einzigartiger und wertvoller Datensätze leistet EnviDat einen Beitrag zur Forschung im Bereich der Umweltwissenschaften.

it: EnviDat è il portale dati del WSL che fornisce un accesso unificato e gestito ai dati di monitoraggio ambientale e di ricerca. Il portale ha la capacità di ospitare e pubblicare serie di dati preziosi e unici che vengono condivisi in tutto il mondo. Così facendo, EnviDat promuove la ricerca e contribuisce al progresso della scienza ambientale.

political_level: other

wsl

capacity: public

type: organization

approval_status: approved

key: guid

key: uri

value: Envidat Harvester

en: Data analysis toolkits


maintainer: Frank Techel

en: avalanche-fatalities avalanche-accident avalanche-accident-statistics long-term-trends avalanche-statistics

en: Data avalanche fatalities 1969/1970 - 2014/2015

media_type: text/plain

en: The data comprises avalanche fatality data from the Alpine regions in the seven countries Austria, France, Germany, Italy, Liechtenstein, Slovenia, and Switzerland.

format: TXT

mimetype: text/plain

en:
During the last 45 years, about 100 people lost their lives in avalanches in the European Alps each year. Avalanche fatalities in settlements and on transportation corridors have considerably decreased since the 1970s. In contrast, the number of avalanche fatalities during recreational activities away from avalanche-secured terrain doubled between the 1960s and 1980s and has remained relatively stable since, despite a continuing strong increase in winter backcountry recreational activities.

Data complementing Figure 2 in:
_"Avalanche fatalities in the European Alps: long-term trends and statistics"_, by Techel, F., Jarry, F., Kronthaler, G., Mitterer, S., Nairz, P., Pavšek, M., Valt, M., and Darms, G.

Data description:
please refer to section 2 (Data and Methods) in the mentioned publication

avalanche-accident

display_name: avalanche-accident

avalanche-accident-statistics

display_name: avalanche-accident-statistics

avalanche-fatalities

display_name: avalanche-fatalities

avalanche-statistics

display_name: avalanche-statistics

long-term-trends

display_name: long-term-trends

en: Avalanche fatalities in the European Alps (1969/1970 - 2014/2015)


en: relative-humidity meteo temperature long-term par climate-change swiss-forest-lab precipitation meteorology wind-speed

en: Site photo of the field station (LAF)

en: Site photo of the field station from the side.

mimetype: image/jpeg

en: Field station data (LAF)

media_type: text/csv

en: Data file.

format: CSV

mimetype: text/csv

en: Forest station data (LAB)

en: Data plot of the forest station (LAB)

en: Data overview for air temperature, relative humidity, precipitation, PAR, wind speed.

en: Data plot of the field station (LAF)

en: Site aerial photo (Lausanne)

en: Site aerial photo from both Lausanne stations. Source: Google. 2016. Site aerial photo from both Lausanne stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Site photo of the forest station (LAB)

en: Site photo of the fforest station from the side.

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Lausanne in Switzerland where one station is located within a natural mixed forest stand (LAB) with European beech (_Fagus sylvatica_; 160-170 yrs), European silver fir (_Abies alba_; 160-170 yrs) and Norway spruce (_Picea abies_; 160-170 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, LAF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Lausanne is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

par

display_name: par

precipitation

display_name: precipitation

relative-humidity

display_name: relative-humidity

swiss-forest-lab

display_name: swiss-forest-lab

temperature

display_name: temperature

wind-speed

display_name: wind-speed

en: Lausanne, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1996 onwards


en: detritus yellowstone wolf carcass decomposition carrion biogeochemical-cycling soil-biodiversity fungi bacteria

en:
Site	ID for different carcasses across the Yellowstone Landscape
Location	Sampling location, either under carcass or control plot
Species	Indicating whether carcass was from bison or elk
Gender	Gener of carcass; F = female, M = male, ? = unknown
Lat	Latitude
Long	Longitude
DSK	Days since kill; in days
Soil.N	Soil N concentration (%)
Soil.C	Soil C concentration (%)
Veg.C	Vegetation C concentration (%); dominant grasses sampled
Veg.N	Vegetation N concentration (%); dominant grasses sampled
Veg.CtoN	Vegetation C to N ratio; dominant grasses sampled
Soil.resp	Soil respiration (g m-2 h-1)
soil.temp	Soil temperature (°C)
Soil.moist	Soil moisture (volumentric; %)
CRR	Cotton rotting rate
bacteria.richness	Bacteria richness
bacteria.diversity	Bacteria diverstiy
fungi.richness	Fungi richness
fungi.diversity	Fungi diversity
bacteria.abundance	Bacteria abundance
fungi.abundance	Fungi abundance

format: XLS

en:
This dataset contains all data on which the following publication below is based.

Paper Citation:

Risch, A.C., Frossard, A., Schütz, M., Frey, B., Morris, A.W., Bump, J.K. (accepted) Effects of elk and bison carcasses on soil microbial communities and ecosystem functions in Yellowstone, USA. (accepted). Functional Ecology
doi: ...

Methods
Study area and study sites
This study was conducted in YNP’s Northern Range (NR), located in north-western Wyoming and south-western Montana, USA (~44.9163° N, 110.4169° W). The NR expands over ~1000 km2 and features long cold winters and short dry summers. Grasslands and shrublands dominate the NR that is the home of large migratory herds of bison (winter counts 2017: ~3919 individuals; Geremia, Wallen, & White, 2017) and elk (~5349 individuals) as well as their main predators, approximately five packs of wolves with a total of 33 individuals (Smith et al., 2017). As part of a long-term research program within YNP, wolf predation has been studied since their reintroduction in 1995.
For our study, we received ground-truthed coordinates of bison and elk carcasses from winter 2016/17 (November 2016 through April 2017) from the YNP Wolf Project. Between June 20 and July 1, 2017, we visited 24 carcasses in total. At five sites, we could not sample as the carcasses were no longer found. In total we located remains (hairmats, rumen content, bones, teeth) of 19 adult male and female carcasses (7 bison, 12 elk; Supplementary Table 1). Live body weights of adult bison and elk are approximately 730 kg (male bison), 450 kg (female bison), 330 kg (male elk), and 235 kg (female elk, Meagher, 1973; Quimby & Johnson, 1951).
The kills and subsequent consumption happened between 34 and 173 days prior to our sampling (hereafter “days since kill”, DSK), for which we accounted in our statistics. Note that wolves and other scavengers consumed the soft tissue of the carcasses quickly, hence, there is close to no soft tissue left for decomposition as compared to an intact body left on the soil surface. The 19 carcass sites covered the extent of YNP’s NR, with both bison and elk carcasses showing similar distributions; elevation ranged from 1703 to 2884 m a.s.l. (Supplementary Fig 1 & Supplementary Table 1). The carcasses were all located in grassland or sage-brush shrubland, with or without sparsely scattered trees, and both bison and elk carcasses showed the same distribution of DSK. At each study site, we selected a reference plot (hereafter “control”) that was of comparable size, slope aspect and vegetation to the carcass location (hereafter “carcass”). The control was at least 10 m away (Danell, Berteaux, & Brathen, 2002; Melis et al., 2007) from the carcass itself to ensure the absence of potential direct and indirect carcass effects (paired design; (Bump, Webster, et al., 2009; Bump, Peterson, et al., 2009).

Ecosystem functions and soil properties
We randomly collected 50 g of mineral soil from three locations on both control and carcass plots to a depth of 5 cm with sterile techniques and gently mixed the material to obtain a composite sample. Half the soil sample was immediately bagged in plastic bags (whirl packs), stored in a cooler with ice packs (~5 ºC), sieved (2-mm) and frozen within 4-6 hours of collection to assess soil microbial communities. For this purpose, we extracted total genomic DNA from 0.5 g soil using the PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany). DNA concentrations were measured using PicoGreen (Molecular Probes, Eugene, OR, USA). PCR amplifications of partial bacterial small-subunit ribosomal RNA genes (region V3–V4 of 16S rRNA) and fungal ribosomal internal transcribed spacers (region ITS2) were performed as described previously (Frey et al., 2016). Each sample consisting of 40 ng DNA was amplified in triplicate and pooled before purification with Agencourt AMPure XP beads (Beckman Colter, Berea, CA, USA) and quantified with the Qubit 2.0 fluorometric system (Life Technologies, Paisley, UK). Amplicons were sent to the Genome Quebec Innovation Center (Montreal, Canada) for barcoding using the Fluidigm Access Array technology and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA).
Quality control of bacterial and fungal reads was performed using a customized pipeline (Supplementary Table 2; Frey et al., 2016). Paired-ends reads were matched with USEARCH (Edgar & Flyvbjerg, 2015), substitution errors were corrected using Bayeshammer (Nikolenko, Korobeynikov, & Alekseyev, 2013) and PCR primers were trimmed (allowing for 1 mismatch, read length >300 bp for 16S and >200 bp for ITS primers) using Cutadapt (M. Martin, 2011). Sequences were dereplicated and singleton reads removed prior to clustering into operational taxonomic units (OTUs) at 97% identity using USEARCH (Edgar, 2013). The remaining centroid sequences were tested for the presence of ribosomal signatures using Metaxa2 (Bengtsson-Palme et al., 2015) or ITSx (Bengtsson-Palme et al., 2013). Taxonomic assignments of the OTUs were obtained using Bayesian classifier (Wang, Garrity, Tiedje, & Cole, 2007) with a minimum bootstrap support of 60% implemented in mothur (Schloss et al., 2009) by querying the bacterial and fungal reads against the SILVA Release 128 (Quast et al., 2013) and UNITE 8.0 (Abarenkov et al., 2010) reference databases for 16S and ITS OTUs, respectively.
Abundances of the bacterial 16S rRNA gene and fungal ITS amplicon were determined by quantitative real-time PCR (qPCR) on an ABI7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) as described previously (Frossard et al., 2018). The same primers (without barcodes) and cycling conditions as for the sequencing approach were used for the 16S and ITS qPCR. Three standard curves per target region were obtained using tenfold serial dilutions of plasmids generated from cloned targets (Frey, Niklaus, Kremer, Lüscher, & Zimmermann, 2011). Data were converted to represent mean copy number of targets per gram of soil (dry weight).
The other half of the soil sample was bagged in paper, dried to constant weight at 60°C, passed through a 2 mm sieve and analyzed for total C and N concentration with a CE Instruments NC 2100 soil analyzer (CE Elantech Inc., Lakewood NJ, USA). We also collected 20 mature and undamaged leaves of the dominant grass species growing on control and carcass sites, but taxa were not recorded. The plant material was dried at 60°C, finely ground till homogenized and also analyzed to obtain total C and N concentrations. Soil temperature (10 cm depth) was measured with a waterproof digital thermometer (Barnstead International, Dubuque IA, USA) at three locations each at the control and carcass site. Soil moisture (0 – 10 cm depth) was measured with time domain reflectometry (Field-Scout TDR-100; Spectrum Technologies, Plainfield IL, USA) at five randomly chosen points on control and carcass sites. We measured soil respiration at five randomly chosen points at both control and carcass sites with a PP-Systems SRC-1 soil respiration chamber (closed circuit) attached to a PP-Systems EGM-4 infrared gas analyzer (PP-Systems, Amesbury, MA, USA). For each measurement the soil chamber (15 cm high; 10 cm diameter) was tightly placed on the soil surface, after clipping plants to avoid measuring plant respiration or photosynthesis. Measurements were conducted over 120 s.
In addition, we assessed the decomposition rates of standardized OM using the cotton strip assay (Latter & Howson, 1977; Latter & Walton, 1988). Cotton cloth tensile strength loss (CTSL) is a measure of decomposition, and an index to express the combined effect of soil microclimatic, physical, chemical and biological properties on decomposition while accounting for OM quality (Latter & Walton, 1988; Risch, Jurgensen, & Frank, 2007; Withington & Sanford Jr., 2007). We placed five 20 cm wide x 13 cm long sheets of 100% unbleached cotton cloth (American Type SM 1/18’’, Warp: 34/1, Weft: 20/1, Weave plain, 29.5 picks/cm warp, 22 picks/cm weft, 237 g/m2; Daniel Jenny & Co., Switzerland;) at each carcass and control site vertically into the soil by making slits with a flat spade to a depth of 12 cm. We inserted each cloth with the spade, and then pushed the slit closed to assure tight contact with the soil. The cloths were retrieved after 18 to 27 days. After retrieval, the cloths were air-dried, remaining soil gently removed by hand, and 1.5 cm wide strips were cut at the 3.5-5.0 cm (top) and the 9-10.5 cm (bottom) soil depth. The strips were equilibrated at 50 % relative humidity and 20°C for 48 hours (climate chamber) prior to strength testing (Scanpro Awetron TH-1 tensile strength tester; AB Lorentzen and Wettre, Kista, Sweden). Cotton rotting rate (CRR) = (CTScontrol - CTSfinal/CTSfinal)1/3 * (365/t), where CTScontrol is the cotton tensile strength of a control cloth and CTSfinal the cotton tensile strength of the incubated sample, t is the incubation period in days. Control cloths were inserted into the ground and immediately retrieved to account for tensile strength loss associated with cloth insertion. We averaged the CRR of top and bottom strips for further analyses as no difference was found between the two. All sampling and cloth insertion took place between June 20 and July 1, 2017, cloths were retrieved between July 17 and 20, 2017. Soil respiration, average CRR, vegetation N concentration and vegetation C:N ratio are defined as ecosystem functions, soil C and N concentration, soil temperature and moisture as soil abiotic properties, and bacterial and fungal richness (number of taxa), diversity (Shannon) and abundance as soil biotic properties.

Statistical analyses
Univariate analyses for ecosystem functions, soil biotic and abiotic properties
We tested whether individual ecosystem functions, soil biotic and abiotic properties differed between carcass and control (“Location”), bison and elk (“Species”) and days since kill (“DSK”). For this purpose, we used linear mixed effect models (LMM, “nlme” package v 3.1 – 131.1 in R v 3.4.4; Pinheiro, Bates, DebRoy, & Sarkar, 2018; R Core Team, 2019) with Location, Species, Location x Species and DSK as fixed effects. Site was included as random effect to account for the paired design. We developed a separate model for all dependent variables. All but bacterial richness, fungal richness, fungal diversity and vegetation N concentration were natural-log transformed to meet model assumptions. For each LMM, we calculated contrasts to assess the specific comparisons we were interested in with the “lsmeans” package v 2.27-62 (Lenth & Love, 2018): 1) carcass vs control, 2) carcass bison vs control bison, and 3) carcass elk vs control elk. We also tested whether we had differences between bison and elk carcasses or the sites where bison and elk were killed and included contrasts 4) carcass bison vs carcass elk and 5) control bison vs control elk.
We calculated the log response ratio (LRR = ln[carcass/control]) to obtain carcass effects for all variables for both species separately. LRR < 0 indicates higher value at control compared to carcass, LRR > 0 indicates higher values at carcass compared control. We used LRRs for visualization and to assess spatial patterns in carcass effects across YNP. For this purpose we calculated the Moran’s I statistic for each ecosystem function, soil biotic and abiotic property based on a latitude-longitude matrix with the “moran.test” function in the “spdep” package version 1.1-3 (Bivand et al., 2019).
Multivariate analyses
Rare OTUs, defined as OTUs with a low abundance of reads, were retained in multivariate methods because they only marginally influence these analyses (Gobet, Quince, & Ramette, 2010). Bray–Curtis dissimilarity matrices were generated based on square-root-transformed matrices. We used Principal Coordinate Analyses (PCoA) to assess how soil bacterial and fungal communities differed between control and carcass of bison and elk (“vegan” package v 2.5-4, Oksanen et al., 2019). We then extracted PCoA axes scores 1 and 2 and used LMM (“nlme” package) with Location, Species, Location x Species and DSK as fixed effects. Site was, again, included as random effect. We again calculated the contrasts as described above using the “lsmeans” package. We also assessed how ecosystem functions, and soil abiotic and biotic properties were related to the soil bacteria and fungi community structure associated with bison and elk control and carcasses using the “envfit” function in the “vegan” package (Oksanen et al., 2019).
Indicator species analyses were performed using the multipatt function implemented in the “indicspecies” package version 1.7.6 with 100000 permutations (De Caceres & Jansen, 2016). This step allowed to identify OTUs that led to changes in multivariate patterns between control and carcass of both bison and elk separately (De Cáceres, Legendre, & Moretti, 2010). The multipatt function uses a point biserial correlation coefficient statistical test. Indicator OTUs were defined as bacterial and fungal OTUs with more than 50 sequences, i.e., removing rare taxa and taxa with low abundances containing little indicator information (Rime et al., 2015) and that were significantly correlated with Location (p < 0.05, correlation coefficient > 0.3). A heatmap of these OTUs were generated with the vegan and ggplot2 packages. The indicator analyses were performed in R version 3.3.3 (R Core Team, 2017).

References
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Bengtsson-Palme, J., Hartmann, M., Eriksson, K. M., Pal, C., Thorell, K., Larsson, D. G. J., & Nilsson, R. H. (2015). metaxa2: improved identification and taxonomic classification of small and large subunit rRNA in metagenomic data. Molecular Ecology Resources, 15(6), 1403–1414. doi:10.1111/1755-0998.12399
Bengtsson-Palme, J., Ryberg, M., Hartmann, M., Branco, S., Wang, Z., Godhe, A., … Nilsson, R. H. (2013). Improved software detection and extraction of ITS1 and ITS2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data. Methods in Ecology and Evolution, 4(10), 914–919. doi:10.1111/2041-210X.12073
Bivand, R., Altman, M., Anselin, L., Assuncao, R., Berke, O., Blanchet, G., … Yu, D. (2019). spdep: Spatial dependence, weighthing schemes, statistics. R package version 1.1-3.
Bump, J. K., Peterson, R. O., & Vucetich, J. A. (2009). Wolves modulate soil nutrient heterogeneity and foliar nitrogen by configuring the distribution of ungulate carcasses. Ecology, 90(11), 3159–3167.
Bump, J. K., Webster, C. R., Vucetich, J. A., Peterson, R. O., Shields, J. M., & Powers, M. D. (2009). Ungulate carcasses perforate ecological filters and create biogeochemical hotspots in forest herbaceous layers allowing trees a competitive advantage. Ecosystems, 12(6), 996–1007. doi:10.1007/s10021-009-9274-0
Danell, K., Berteaux, D., & Brathen, K. A. (2002). Effect of muskox carcasses on nitrogen concentration in tundra vegetation. Arctic, 55(4), 389392.
De Caceres, M., & Jansen, F. (2016). indicspecies: relationship between species and groups of species. R package version 1.7.6.
De Cáceres, M., Legendre, P., & Moretti, M. (2010). Improving indicator species analysis by combining groups of sites. Oikos, 119(10), 1674–1684. doi:10.1111/j.1600-0706.2010.18334.x
Edgar, R. C. (2013). UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods, 10, 996.
Edgar, R. C., & Flyvbjerg, H. (2015). Error filtering, pair assembly and error correction for next-generation sequencing reads. Bioinformatics, 31(21), 3476–3482. doi:10.1093/bioinformatics/btv401
Frey, B., Niklaus, P. A., Kremer, J., Lüscher, P., & Zimmermann, S. (2011). Heavy-machinery traffic impacts methane emissions as well as methanogen abundance and community structure in oxic forest soils. Applied and Environmental Microbiology, 77(17), 6060–6068. doi:10.1128/AEM.05206-11
Frey, B., Rime, T., Phillips, M., Stierli, B., Hajdas, I., Widmer, F., & Hartmann, M. (2016). Microbial diversity in European alpine permafrost and active layers. FEMS Microbial Ecology, 92(3), fiw018.
Frossard, A., Donhauser, J., Mestrot, A., Gygax, S., Bååth, E., & Frey, B. (2018). Long- and short-term effects of mercury pollution on the soil microbiome. Soil Biology and Biochemistry, 120, 191–199. doi:https://doi.org/10.1016/j.soilbio.2018.01.028
Geremia, C., Wallen, R., & White, P. J. (2017). Status report of the Yellowstone bison population, September 2017. Yellowstone National Park, Mammoth, WY, USA: National Park Service, Yellowstone Center for Resources.
Gobet, A., Quince, C., & Ramette, A. (2010). Multivariate cutoff level analysis (MultiCoLA) of large community data sets. Nucleic Acids Research, 38(15), e155–e155. doi:10.1093/nar/gkq545
Latter, P., & Howson, G. (1977). The use of cotton strips to indicate cellulose decomposition in the field. Pedobiologia, (17), 145–155.
Latter, P., & Walton, D. (1988). The cotton strip assay for cellulose decomposition studies in soil: history of the assay and development. In Cotton strip assay: an index for decomposition in soils (pp. 7–9). ITE Symposium, Institute of Terrestrial Ecology, Natural Environment Research Council, UK.
Lenth, R., & Love, J. (2018). lsmeans: least-squares means. R package version 2.27-62.
Martin, M. (2011). Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.Journal, 17(1), 10–12.
Meagher, M. M. (1973). The bison of Yellowstone National Park. NPS Scientific Monograph (Vol. 1). National Park Service, Yellowstone Center for Resources.
Melis, C., Selva, N., Teurlings, I., Skarpe, C., Linnell, J. D. C., & Andersen, R. (2007). Soil and vegetation nutrient response to bison carcasses in Białowieża Primeval Forest, Poland. Ecological Research, 22(5), 807–813. doi:10.1007/s11284-006-0321-4
Nikolenko, S. I., Korobeynikov, A. I., & Alekseyev, M. A. (2013). BayesHammer: Bayesian clustering for error correction in single-cell sequencing. BMC Genomics, 14(1), S7. doi:10.1186/1471-2164-14-S1-S7
Oksanen, J., Blanchet, F. G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., … Wagner, H. H. (2019). vegan: community ecology package. R package version 2.5-4.
Pinheiro, J., Bates, D., DebRoy, S., & Sarkar, D. (2018). nlme: Linear and nonlinear mixed effect models. R package version 3.1-131.1.
Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., … Glöckner, F. O. (2013). The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Research, 41(Database issue), D590–D596. doi:10.1093/nar/gks1219
Quimby, D. C., & Johnson, D. E. (1951). Weights and measurements of Rocky Mountain elk. Journal of Wildlife Management, 15, 57–62.
R Core Team. (2017). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Zurich, Switzerland.
R Core Team. (2019). R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing.
Rime, T., Hartmann, M., Brunner, I., Widmer, F., Zeyer, J., & Frey, B. (2015). Vertical distribution of the soil microbiota along a successional gradient in a glacier forefield. Molecular Ecology, 24(5), 1091–1108. doi:10.1111/mec.13051
Risch, A. C., Jurgensen, M. F., & Frank, D. A. (2007). Effects of grazing and soil micro-climate on decomposition rates in a spatio-temporally heterogeneous grassland. Plant and Soil, 298(1–2), 191–201. doi:10.1007/s11104-007-9354-x
Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., … Weber, C. F. (2009). Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology, 75(23), 7537–7541. doi:10.1128/AEM.01541-09
Smith, D., Stahler, D., Cassidy, K., Stahler, E., Metz, M., Cassidy, B., … Cato, E. (2018). Yellowstone National Park wolf project annual report 2017. Yellowstone National Park, Mammoth, WY, USA: National Park Service, Yellowstone Center of Resources.
Wang, Q., Garrity, G. M., Tiedje, J. M., & Cole, J. R. (2007). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology, 73(16), 5261–5267. doi:10.1128/AEM.00062-07
Withington, C., & Sanford Jr., R. (2007). Decomposition rates of buried substances increase with altitude in a forest-alpine tundra ecotone. Soil Biology and Biochemistry, (39), 68–75.

Please cite this paper together with the citation for the datafile.

bacteria

display_name: bacteria

biogeochemical-cycling

display_name: biogeochemical-cycling

carcass

display_name: carcass

carrion

display_name: carrion

decomposition

display_name: decomposition

detritus

display_name: detritus

fungi

display_name: fungi

soil-biodiversity

display_name: soil-biodiversity

wolf

display_name: wolf

yellowstone

display_name: yellowstone

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Elk and bison carcasses in Yellowstone, USA


en: climate-change long-term meteo swiss-forest-lab relative-humidity wind-speed temperature meteorology precipitation par

en: Site photo of the field station (NEF)

en: Site photo of the field station from the side.

en: Data plot of the field station (NEF)

en: Field station data (NEF)

en: Data file.

en: Site aerial photo (Neunkirch)

en: Site aerial photo from both Neunkirch stations. Source: Google. 2016. Site aerial photo from both Neunkirch stations. Retrieved November 11, 2016, from https://www.google.ch/maps

en: Forest station data (NEB)

en: Site photo of the forest station (NEB)

en: Site photo of the forest station from below (viewing upwards the slope).

en: Data plot of the forest station (NEB)

en: High quality meteorological data are needed for long-term forest ecosystem research, particularly in the light of global change. The long-term data series published here comprises almost 20 years of measurements for two meteorological stations in Neunkirch in Switzerland where one station is located within a natural deciduous forest stand (NEB) with European beech (_Fagus sylvatica_; 160 yrs), sycamore maple (_Acer pseudoplatanus_; 160 yrs) and lime trees (_Tilia sp._; 110 yrs) as dominant tree species. A second station is situated in the very vicinity outside of the forest (field station, NEF). The meteorological time series are presented in hourly time resolution of air temperature, relative humidity, precipitation, photosynthetically active radiation (PAR) and wind speed. Neunkirch is part of the Long-term Forest Ecosystem Research Programme (LWF) established and maintained by the Swiss Federal Research Institute WSL.

climate-change

display_name: climate-change

long-term

display_name: long-term

meteo

display_name: meteo

meteorology

display_name: meteorology

en: Neunkirch, Switzerland: Long-term forest meteorological data from the Long-term Forest Ecosystem Research Programme (LWF), from 1997 onwards


maintainer: Izabela Bujak-Ozga

en: dynamics catchment precipitation catchment-data channel water-level drainage drainage-network

en: Documentation for the Short-term Drainage Density Dynamics Dataset for the Haute-Mentue Catchment (dataset_of_flowing_drainage_network_lengths_water_levels_and_precipitation_for_the_haute-mentue_catchment.csv). This document provides a detailed description of the collected data, the locations where it was recorded, the data format, and the curation process applied.

en: The dataset consists of time series of measured surface and groundwater levels, calculated flowing drainage network lengths, and nearby precipitation data for two sub-catchments of the Haute-Mentue catchment, Switzerland (Summer-Autumn 2022). The details of the dataset are described in the provided documentation (short-term_drainage_density_dynamics_dataset_documentation_for_the_haute-mentue_catchment.pdf)

en: The dataset contains time series of water levels, precipitation measured in the two sub-catchments of the Haute-Mentue catchment and its vicinity during summer and autumn 2022, as well as flowing drainage network lengths calculated for these areas using the CEASE method developed by the authors. Detailed description of the dataset is provided in the documentation.

catchment

display_name: catchment

catchment-data

display_name: catchment-data

channel

display_name: channel

drainage

display_name: drainage

drainage-network

display_name: drainage-network

dynamics

display_name: dynamics

precipitation

display_name: precipitation

water-level

display_name: water-level

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal


en: forest-inventory mobile-laser-scanning terrestrial-laser-scanning 3d-point-cloud lidar structure-from-motion unmanned-aerial-vehicle-laser-scanning terrestrial-photogrammetry

media_type: application/zip

format: ZIP

en:
Close Range Remote Sensing Benchmark for different LiDAR and photogrammetric Sensors in a mixed temperate forest.
Benchmarks are needed to evaluate the performance of different close-range remote sensing devices and approaches, both in terms of efficiency as well as accuracy. In this study we evaluate the performance of two terrestrial (TLS), one handheld mobile (PLS) and two drone based (UAVLS) laser scanning systems to detect trees and extract the diameter at breast height (DBH) in three plots with a steep gradient in tree and understorey vegetation density. As a novelty, we also tested the acquisition of 3D point-clouds using a low-cost action camera (GoPro) in conjunction with the Structure from Motion (SfM) technique and compared its performance with those of the more costly LiDAR devices.

forest-inventory

display_name: forest-inventory

lidar

display_name: lidar

mobile-laser-scanning

display_name: mobile-laser-scanning

structure-from-motion

display_name: structure-from-motion

terrestrial-laser-scanning

display_name: terrestrial-laser-scanning

terrestrial-photogrammetry

display_name: terrestrial-photogrammetry

unmanned-aerial-vehicle-laser-scanning

display_name: unmanned-aerial-vehicle-laser-scanning

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Ramerenwald Close Range Remote Sensing Benchmark


maintainer: Yohann Chauvier

en: point-process-model target-group cluster independent-dataset survey-effort random-stratified-sampling environmental-stratification background-data covariate-correction plant-species

en: Non-copyright data, scripts, diversity outputs

en:
Aim: While species distribution models (SDMs) are standard tools to predict species distributions, they can suffer from observation and sampling biases, particularly presence-only SDMs that often rely on species observations from non-standardized sampling efforts. To address this issue, sampling background points with a target-group strategy is commonly used, although more robust strategies and refinements could be implemented. Here, we exploited a dataset of plant species from the European Alps to propose and demonstrate efficient ways to correct for observer and sampling bias in presence-only models.

Innovation: Recent methods correct for observer bias by using covariates related to accessibility in model calibrations (classic bias covariate correction, Classic-BCC). However, depending on how species are sampled, accessibility covariates may not sufficiently capture observer bias. Here, we introduced BCCs more directly related to sampling effort, as well as a novel corrective method based on stratified resampling of the observational dataset before model calibration (environmental bias correction, EBC). We compared, individually and jointly, the effect of EBC and different BCC strategies, when modelling the distributions of 1’900 plant species. We evaluated model performance with spatial block split-sampling and independent test data, and assessed the accuracy of plant diversity predictions across the European Alps.

Main conclusions: Implementing EBC with BCC showed best results for every evaluation method. Particularly, adding the observation density of a target group as bias covariate (Target-BCC) displayed most realistic modelled species distributions, with a clear positive correlation (r≃0.5) found between predicted and expert-based species richness. Although EBC must be carefully implemented in a species-specific manner, such limitations may be addressed via automated diagnostics included in a provided R function. Implementing EBC and bias covariate correction together may allow future studies to address efficiently observer bias in presence-only models, and overcome the standard need of an independent test dataset for model evaluation.

background-data

display_name: background-data

cluster

display_name: cluster

covariate-correction

display_name: covariate-correction

environmental-stratification

display_name: environmental-stratification

independent-dataset

display_name: independent-dataset

plant-species

display_name: plant-species

point-process-model

display_name: point-process-model

random-stratified-sampling

display_name: random-stratified-sampling

survey-effort

display_name: survey-effort

target-group

display_name: target-group

en: Novel methods to correct for observer and sampling bias in presence-only species distribution models


maintainer: Henning Löwe

en: 3d-ct-images unconfined-compression-experiments spherical-model-snow discrete-element-modelling microstructure snow-mechanics

en: Data Structure (Python Notebook)

en: Data Access

en:
For the investigation of microstructural and mechanical properties of snow unconfined compression experiments and 3D computed tomography (CT) imaging were performed on sintered rounded grain snow and spherical model snow. The spherical model snow was generated to create geometrically simplified, well-defined microstructures for calibration of numerical models, such as discrete element models (DEM) in which the microstructure is represented by spherical particles.
In the experiments, microstructural variation was created by varying the sintering time (contact size) and the density of the ice sphere samples (number of contacts). The 3D CT images allow for a complete reconstruction of the entire experimental sample (cylindrical sample dimension: diameter = 33.6 mm; height = 14 mm).

discrete-element-modelling

display_name: discrete-element-modelling

microstructure

display_name: microstructure

snow-mechanics

display_name: snow-mechanics

spherical-model-snow

display_name: spherical-model-snow

unconfined-compression-experiments

display_name: unconfined-compression-experiments

en: Unconfined compression experiments and 3D CT images of spherical model snow and RG snow samples


maintainer: Paraskevi Georgakaki

en: jungfraujoch wrf swiss-alps blowing-snow liquid-water-content ice-crystal-number-concentration secondary-ice-production ice-water-content

en: WRF outputs

en:
The .zip file contains all the NetCDF outputs produced by the mesoscale Weather Research and Forecasting (WRF) model (version: 4.0.1). Two case studies were simulated in this study, between 25-28 January 2014 (00:00 UTC) and 29 January - 1 February 2014 (00:00 UTC), respectively. The former is denoted as "NW" and the latter as "SE", owing to the prevailing wind conditions at the Jungfraujoch (JFJ) station.

The outputs come from the innermost domain of the simulations with a spatial resolution of 1 km and are extracted from the nearest to JFJ model grid point (46.55°N 7.98°E). The dimensions of each model variable is [145,100], which corresponds to time and height, respectively. The frequency of the outputs is 30 min. Note that the first 24 hours of each simulation (i.e., the first 48 outputs) were considered spin up time.

The following sets of sensitivity simulations were presented in this study:

* CNTRL_NW.nc, CNTRL_SE.nc : Sensitivity simulations performed with the default version of WRF
* DS_PHILL_NW.nc, DS_PHILL_SE.nc : Sensitivity simulations including a description of the droplet shattering process following Phillips et al. (2018)
* DS_SULL_NW.nc, DS_SULL_SE.nc : Sensitivity simulations including a description of the droplet shattering process following Sullivan et al. (2018)
* PHILL_NW.nc, PHILL_SE.nc : Sensitivity simulations including a description of the ice-ice collisional break-up process following Phillips et al. (2017)
* TAKAH_NW.nc, TAKAH_SE.nc : Sensitivity simulations including a description of the ice-ice collisional break-up process following Sullivan et al. (2018) based on Takahashi et al. (1995)
* TAKAHrim_NW.nc, TAKAHrim_SE.nc : Same set-up as before but applied only to collisions between rimed ice particles
* BIPS_Xk0_DY_NW.nc, BIPS_Xk0_DY_SE.nc : Sensitivity simulations including a source of Xk0 (either 10.0 or 100.0 L⁻¹) ice crystals with sizes of DY (either 10 or 100 μm) in the first atmospheric level of the model
* BIPS100_PHILL_NW.nc, BIPS100_PHILL_SE.nc : Sensitivity simulations where the combined effect of blowing snow and ice-ice collisional break-up is examined

en: Observational data

en:
The .zip file contains 6 .csv files including all microphysical measurements taken at the Jungfraujoch station during the two periods of interest:

* 25-28 January 2014 : northwesterly-winds (NW)
* 29 January - 1 February 2014 : southeasterly-winds (SE)

The total Ice Crystal Number Concentration (ICNC; reported in L⁻¹) is measured by the two-dimensional stereo hydrometeor spectrometer (2D-S; Lawson et al., 2006) over the size range 10-1280 μm. The total ice water content (IWC; reported in gm⁻³) is also derived by the 2D-S data using the Brown and Francis (1995) mass-diameter relationship. Finally, the quantification of the liquid water content (LWC; reported in gm⁻³) is based on the liquid droplet size distribution data derived from a DMT cloud droplet probe (CDP; Lance et al., 2010) over the size range 2-50 μm. The frequency of the measurements in the .csv files is 30 min, to match the output frequency of the model. Datetime is also reported in the files (DD/MM/YYYY h:mm) in UTC.

Acknowledgment: Collection and pre-processing of the microphysical measurements has been performed by Gary Lloyd (gary.lloyd@manchester.ac.uk). More details about the instrumentation during the CLACE2014 campaign can be found in Lloyd et al. (2015).

en:
This repository contains all WRF model outputs and observational data sets used for the paper:
Georgakaki, P., Sotiropoulou, G., Vignon, É., Billault-Roux, A.-C., Berne, A., and Nenes, A.: Secondary ice production processes in wintertime alpine mixed-phase clouds, Atmos. Chem. Phys. Discuss., https://doi.org/10.5194/acp-2021-760, in review, 2021.

blowing-snow

display_name: blowing-snow

ice-crystal-number-concentration

display_name: ice-crystal-number-concentration

ice-water-content

display_name: ice-water-content

jungfraujoch

display_name: jungfraujoch

liquid-water-content

display_name: liquid-water-content

secondary-ice-production

display_name: secondary-ice-production

swiss-alps

display_name: swiss-alps

wrf

display_name: wrf

en: Data for the publication "Secondary ice production processes in wintertime alpine mixed-phase clouds"


restoring-grassland-multifunctionality

title_for_slug: restoring-grassland-multifunctionality

en: ecosystem-multifunctionality mineralization bacteria grassland restoration biodiversity nematodes plants soil-biodiversity fungi

en: Functional diversity above_belowground fauna

en: Please note that C.sto and Corg have been corrected.

en: Please note that EMF, MF.below, MF.abio and Soil.hetero have been corrected.

en:
Please cite this paper together with the citation for the datafile.

Resch, M. C., Schütz, M., Buchmann, N., Frey, B., Graf, U., van der Putten, W. H., Zimmermann, S., Risch, A. C. 2021. Evaluating long-term success in grassland restoration – an ecosystem multifunctionality approach. Ecological Applications 31, e02271.

 Study area

The study was conducted in the Canton of Zurich, Switzerland, in and around two nature reserves Eigental and Altläufe der Glatt (47°27’ to 47°29’ N, 8°37’ to 8°32’ E, 417 to 572 m a.s.l.). All studied grasslands were located with a radius of approximately 4 km. Average monthly temperatures range from 0.7 ± 2.0 °C (January) to 19.0 ± 1.5 °C (July), and monthly precipitation range from 60 ± 42 mm (January) to 118 ± 46 mm (July [maxima]; 1989-2017; MeteoSchweiz 2018). In our study, we focused on semi-dry and semi-wet oligo- to mesotrophic grasslands characterized by high plant species richness and groundwater fluctuations throughout the year (Delarze et al. 2015, see also Resch et al. 2019).

 Experimental design and sampling

A large-scale restoration experiment to expand and reconnect isolated remnants of species-rich grasslands was initiated in the nature reserve Eigental in 1990. Twenty hectares of adjacent intensive grasslands were chosen for restoration. In 1995, three restoration methods of increasing intervention intensities were implemented. The goal of all three methods was to lower the availability of soil nutrients and hence, facilitate ecosystem development towards the targeted nutrient-poor grasslands. These methods were: Harvest only (hay harvest twice a year), Topsoil (removal of the nutrient-rich topsoil), and Topsoil+Propagules (topsoil removal combined with the application of hay from target vegetation). Plant biomass harvest (once a year in late summer/early autumn) commenced in Topsoil and Topsoil+Propagules five years after the soils were removed and is still ongoing today. We measured restoration success by comparing the three restoration methods with intensively managed (Initial) and semi-natural grasslands (Target) 22 years after restoration. Initial grassland sites share the same agricultural history as the restored sites: mowing and subsequent fertilizing (manure) up to five times a year, as well as different tillage regimes (Resch et al. 2019). Target sites were the sites from which hay for seeding the Topsoil+Propagules sites was collected. Soil conditions (i.e., soil types, soil texture) were comparable to those found in the restored grasslands (Resch et al. 2019). Additionally, Target sites were selected to represent a variety of semi-natural grasslands, including semi-dry to semi-wet conditions. In Target grasslands, biomass is harvested once a year in late summer or early autumn. Eleven 5 m x 5 m (25 m2) plots were randomly established in each of the five treatments (in total 55 plots; for a detailed map see Neff et al. 2020). An additional 2 m x 2 m (4 m2) subplot was randomly established at least 2 m away from each 25 m2 plot for destructive sampling. Data sampling took place between June and September 2017.

Vegetation properties

All plant species were identified within the 25 m2 plots (nomenclature: Lauber and Wagner 1996) in mid-June 2017 (in total 250 species). Vegetation structure and plant biomass were assessed diagonally on a transect of 2 m x 10 cm within the 25 m2 plot in early July 2017. We measured the maximum and mean height of the vegetation at the start, middle and end of the transect and calculated the standard deviation of these measures to describe vegetation structural heterogeneity (Schuldt et al. 2019). Thereafter, biomass was clipped on the entire transect to 1 cm height, sorted into five functional groups (graminoids, forbs, legumes, litter, and woody species), dried at 60 °C for 48 h, and weighed (Meyer et al. 2015).

 Aboveground arthropods

Aboveground arthropods were sampled at two locations in each 25 m2 plot in early July 2017 (see also Neff et al. 2020). Briefly, two cylindrical baskets (50 cm diameter, 67 cm height; woven fabric) were thrown simultaneously from outside the plot into two opposite corners. A closable mosquito mesh sleeve was mounted to the top of the baskets and an integrated metal ring at the bottom was fixed to the ground with metal stakes to assure that insects could not escape. A suction sampler (Vortis, Burkhard Manufacturing Co. Ltd., Hertfordshire, England) was then inserted into one of the baskets through the opening of the sleeve and the plot was “vacuumed" twice for 105 seconds with a 30 seconds break. The collected animals were immediately transferred into 70% ethanol. Arthropods were sorted and assigned to 23 taxonomic groups. Holometabolic larvae were lumped into one category while hemimetabolic larvae were grouped separately from adults in the respective taxonomic rank. We used mean values of individuals per plot for total abundance. Aboveground arthropod richness was defined by the number of different taxa to lowest taxonomic level (in total 23 taxa). All taxa were assigned to one of five trophic levels: 1) primary producers, 2) primary consumers, 3) secondary consumers, 4) tertiary consumers, and 5) quaternary consumers.

 Belowground fauna

Sampling of all belowground fauna took place in mid-July 2017. Earthworms were sampled in two 30 cm x 30 cm x 20 cm soil monoliths at two opposite corners of the 25 m2 plot (opposite to aboveground arthropod sampling). The excavated soil monolith was broken by hand, all earthworms collected and immediately transferred in a 4% formaldehyde solution. Thereafter, earthworm individuals were identified to species level (in total 10 taxa; Christian and Zicsi 1999) and species assigned to three functional groups (Bouché 1977).
To assess soil arthropod communities, we randomly collected one undisturbed soil core (5 cm diameter, 12 cm depth) in each 4 m2 subplot with a slide hammer corer lined with a plastic sleeve (AMS Samplers, American Falls, Idaho, USA). Soil arthropods were extracted using Berlese-Tullgren funnels (3 mm mesh), starting the day of sampling and lasting 14 days. Individuals were stored in 70% ethanol. Soil arthropods were assigned to 41 taxonomic groups and 4 feeding types. Holometabolic and hemimetabolic larvae were treated as previously described for aboveground arthropods. Belowground arthropod richness refers to the 41 taxonomic groups.

For soil nematode sampling, we randomly collected eight soil cores of 2.2 cm diameter (Giddings Machine Company, Windsor, CO, USA) within each 4 m2 subplot to a depth of 12 cm. The eight cores were combined, gently homogenized, placed in coolers, kept at 4 °C and transported to the laboratory at NIOO in Wageningen (NL) within one week after collection. Free-living nematodes were extracted from 200 g of fresh soil using Oostenbrink elutriator (Oostenbrink 1960) and prepared for morphological identification and quantification as described by Resch et al. (2019). Nematodes were identified to family level (39 taxa) according to Bongers (1988), assigned to 17 functional groups, 5 feeding types and 5 colonizer-persister (C-P) classes (Yeates et al. 1993, Bongers 1990, Resch et al. 2019).

We randomly collected two more soil cores (2.2 cm diameter x 12 cm depth) within each 4 m2 subplot to determine soil microbial communities. Again, the soil cores were combined, homogenized, placed in coolers and transported to the laboratory at WSL in Birmensdorf (Switzerland) where the metagenomic DNA was extracted from 8 g sieved soil (2 mm) using the DNeasy PowerMax Soil Kit (Quiagen, Hilden, NRW, GER) according to the manufacturer`s instructions. PCR amplification of the V3-V4 region of the prokaryotic small-subunit (16S) and the ribosomal internal transcribed spacer region (ITS2) of eukaryotes was performed with 1 ng of template DNA utilizing PCR primers and conditions as previously described (Frey et al., 2016). PCRs were run in triplicates and pooled. The pooled amplicons were sent to the Genome Quebec Innovation Centre (Montreal, QC, Canada) for barcoding using the Fluidigm Access Array technology (Fluidigm) and paired-end sequencing on the Illumina MiSeq v3 platform (Illumina Inc., San Diego, CA, USA). Quality filtering, clustering into operational taxonomic units (OTUs) and taxonomic assignment were performed as described by Frey et al. (2016) and Adamczyk et al. (2019). We used a customised pipeline largely based on UPARSE (Edgar 2013) implemented in USEARCH v. 9.2 (Edgar 2010). After discarding singletons of dereplicated sequences, clustering into OTUs with 97% sequence similarity was performed (Edgar 2013). Quality-filtered reads were mapped on the filtered set of centroid sequences. Taxonomic classification of prokaryotic and fungal sequences was conducted querying against most recent versions of SILVA (v.132, Quast et al. 2013) and UNITE (v.8, Nilsson et al. 2018). Only taxonomic assignments with confidence rankings equal or higher than 0.8 were accepted (assignments below 0.8 set to unclassified). Prokaryotic OTUs assigned to mitochondria or chloroplasts as well as eukaryotic OTUs assigned other than fungi were removed prior to data analysis. In addition, prokaryotic and fungal datasets were filtered to discard singletons and doubletons. Thereafter, OTU abundance matrices were rarefied to the lowest number of sequences per community, to normalize the total number of reads and achieve parity between samples (Prokaryota: 29,843 reads; Fungi: 26,690 reads). Finally, prokaryotic and fungal observed richness (number of OTUs) were estimated (Prokaryota: 14,010 OTUs; Fungi: 5,813 OTUs). For prokaryotes, we distinguished five and for fungi six functional types based on lowest taxonomic resolution (Nguyen et al. 2016, Tedersoo et al. 2014). Belowground taxon richness was defined by the total number of earthworm, arthropod, nematode, fungi, and prokaryote taxa assigned to lowest taxonomic level. Finally, all belowground taxa were assigned to the same five trophic levels as the aboveground arthropods.

 Soil chemical and physical properties, soil nitrogen mineralization

We randomly collected three 5 cm diameter x 12 cm depth soil samples in each 4 m2 subplot with a slide hammer corer (AMS Samplers, American Falls, Idaho, USA), pooled them and then made two subsamples. One was field-fresh and stored at 3 °C until analysis, the other was dried for 48 h at 60 °C and passed through a 4 mm mesh. From the dried sample, we measured soil pH potentiometrically in 0.01 M CaCl2 (soil:solution ratio=1:2; 30 minutes equilibration time). Total and organic carbon content were measured on fine-ground samples (≤ 0.5 mm) by dry combustion using a CN analyzer NC 2500 (CE Instruments, Wigan, United Kingdom). Inorganic carbon of samples with a pH > 6.5 was removed with acid vapor prior to analysis of organic carbon (Walthert et al. 2010). We calculated total soil carbon (C) storage after correcting its content for soil depth, stone content and density of fine earth (see below). Exchangeable cations were determined on another 5 g dry soil sample with 50 mL unbuffered 1 M NH4Cl solution (soil:solution ratio=1:10, end-over-end shaker for 1.5 hours) and measured by an ICP-OES (Optima 7300 DV, Perkin-Elmer, Waltham, Massachusetts, USA). Thereafter, cation exchange capacity (CEC) was calculated as the sum of exchangeable cations and protons (and expressed as mmolc per 1 kg soil) and used to describe nutrient retention capacity in our plots. Concentrations of exchangeable protons were calculated as the difference between total and Al-induced exchangeable acidity as determined by the KCl-method (Thomas 1982).

Ammonium (NH4+) and nitrate (NO3−) were extracted from a 20 g fresh subsample with 80 mL 1M KCl for 1.5 hours on an end-over-end shaker and filtered through ashless folded filter paper (DF 5895 150, ALBET LabScience, Hahnemühle FineArt GmbH, Dassel, Germany). NH4+ concentrations were determined colorimetrically by automated flow injection analysis (FIAS 300, Perkin-Elmer, Waltham, Massachusetts, USA). NO3− concentrations were measured colorimetrically according to Norman and Stucki (1981). Potential soil net nitrogen (N) mineralization was assessed during an 8-week incubation period under controlled moisture (60% of field capacity), temperature (20 °C) and light conditions (dark) in the laboratory. We weighed duplicate samples of fresh soil equivalent to 8 g dry soil (24 h at 104 °C) into 50 mL Falcon tubes. Soil samples were extracted for NH4+ and NO3− at the beginning and after eight weeks as described above. Soil net N mineralization was calculated as the difference between the inorganic nitrogen (NH4+ and NO3−) before and after the incubation (Hart et al. 1994), corrected for the total incubation time and represented per day values expressed as mg N kg-1 soil d-1.

To assess soil physical properties, we randomly collected one undisturbed soil core per 4 m2 subplot (5 cm diameter, 12 cm depth) in a steel cylinder that fit into the slide hammer (AMS Samplers, American Falls, Idaho, USA). The cylinder was capped in the field to avoid disturbance. We then measured field capacity in the laboratory. For this purpose, the cylinder and soil therein were saturated in a water bath and drained on a sand/silt-bed with a suction corresponding to 60 cm hydrostatic head. The moist soil was dried at 105 °C to constant weight. Field capacity was calculated by dividing the mass of water by the total mass of wet soil contained at 60 cm hydrostatic head and used to describe water holding capacity. Thereafter, samples were passed through a 4 mm mesh. Fine-earth and skeleton fractions were weighed separately to assess bulk soil density (fine-earth plus skeleton), density of fine earth, and proportion of skeleton. Particle density was determined with the pycnometer method (Blake and Hartge 1986), and total porosity and proportion of fine pores were calculated (Danielson and Sutherland 1986). Clay, silt, and sand contents were quantified with the sediment method (Gee and Bauder 1986).

Surface and soil temperature (12 cm depth, water-resistant digital pocket thermometer; IP65, H-B Instrument, Trappe, Pennsylvania, USA) as well as volumetric soil moisture content (12 cm depth, time domain reflectometry; Field-Scout TDR 300, Spectrum Technologies, Aurora, Illinois, USA) were measured at five random locations within the 4 m2 subplots every month from June to September. We calculated the standard deviation of each temperature and moisture measure over four months to describe seasonal variations. Slope inclination was determined at plot-level via GPS measurements (GPS 1200, Leica Geosystem, Heerbrugg, Switzerland) and categorized into slope gradient classes according to FAO standards (1990). Thickness of the topsoil horizon (equivalent to Ah or Aa horizon) was determined at one soil monolith (30 x 30 x 30 cm3) per 4 m2 subplot in cm and rounded to next integer.

 References

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Blake, G.R., and K. H. Hartge. 1986. Particle Density. Pages 377-382 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Bongers, T. 1988. De nematoden van Nederland. Stichting Uitgeverij van de Koniklijke Nederlandse Natuurhistorische Verenigung (KNNV), Utrecht.

Bongers, T. 1990. The maturity index: an ecological measure of environmental disturbance based on nematode species composition. Oecologia 83:14-19.

Bouché, M. B. 1977. Strategies lombriciennes. Ecological Bulletins 25:122-132.

Christian, E., and A. Zicsi. 1999. Ein synoptischer Bestimmungsschlüssel der Regenwürmer Österreichs (Oligochaeta: Lumbricidae). Die Bodenkultur 50:121-131.

Danielson, R. E., and P. L. Sutherland. 1986. Porosity. Pages 443-461 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Delarze, R., Y. Gonseth, S. Eggenberg, and M. Vust. 2015. Lebensräume der Schweiz: Ökologie ‐ Gefährdung ‐ Kennarten, Ott Verlag, Bern.

Edgar, R. C. 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:2460–2461.

Edgar, R. C. 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods 10:996–998.

FAO. 1990. Guidelines for soil description, third ed. Land and Water Development Division at the Food and Agriculture Organization of the United Nations (FAO), Rome.

Frey, B., T. Rime, M. Phillips, B. Stierli, I. Hajdas, F. Widmer, and M. Hartmann. 2016. Microbial diversity in European alpine permafrost and active layers. FEMS Microbiology Ecology 92:fiw018.

Gee, G.W., and J. W. Bauder. 1986. Particle-size analysis. Pages 383-411 in A. Klute, editor. Methods of soil analysis: Part 1—Physical and mineralogical methods. Soil Science Society of America (SSSA) Inc., Madison.

Hart, S. C, J. M. Stark, E. A. Davidson, and M. K. Firestone. 1994. Nitrogen mineralization, immobilization, and nitrification. Pages 985-1016 in R. W. Weaver, S. Angle, P. Bottomley, D. Bezdicek, S. Smith, A. Tabatabai, and A. Wollum, editors. Methods of soil analysis: Part 2—Microbiological and biochemical properties. Soil Science Society of America (SSSA) Inc., Madison.

Lauber, K., Wagner, G., 1996. Flora Helvetica. Flora der Schweiz. Haupt Verlag, Bern.

MeteoSchweiz, 2018. Klimabulletin Jahr 2017. MeteoSchweiz, Zürich.

Meyer, S. T., C. Koch, and W. W. Weisser. 2015. Towards a standardized rapid ecosystem function assessment (REFA). Trends in Ecology and Evolution 30:390-397.

Neff, F., M. C. Resch, A. Marty, J. Rolley, M. Schütz, A. C. Risch, and M. M. Gossner. 2020. Long-term restoration success of insect herbivore communities in semi-natural grasslands – a functional approach. Ecological Applications 0:e02133

Nguyen, N.H., Z. W. Song, S. T. Bates, S. Branco, L. Tedersoo, J. Menke, J. S. Schilling, and P. G. Kennedy. 2016. FUNGuild: an open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology 20:241–248.

Nilsson, R. H., K.-H. Larsson, A. F. S. Taylor, J. Bengtsson-Palme, T. S. Jeppesen, D. Schigel, P. G. Kennedy, K. Picard, F. O. Glöckner, L. Tedersoo, I. Saar, U. Kõljalg, and K. Abarenkov. 2018. The UNITE database for molecular identification of fungi: handling dark taxa and parallel taxonomic classifications. Nucleic Acids Research 47:259–264.

Norman, R. J., and J. W. Stucki. 1981. The Determination of Nitrate and Nitrite in Soil Extracts by Ultraviolet Spectrophotometry. Soil Science Society of America Journal 45:347-353.

Oostenbrink, M. 1960. Estimating nematode populations by some selected methods. Pages 81-101 in J. J. Sasser, and W. R. Jenkins, editors. Nematology. Univ. of North Carolina Press, Chapel Hill.

Quast, C., E. Pruesse, P. Yilmaz, J. Gerken, T. Schweer, P. Yarza, J. Peplies, and F. O. Glöckner. 2013. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Research 41:590-596.

Resch, M. C., M. Schütz, U. Graf, R. Wagenaar, W.H. van der Putten, and A. C. Risch. 2019. Does topsoil removal in grassland restoration benefit both soil nematode and plant communities? Journal of Applied Ecology 56:1782-1793.

Schuldt, A., A. Ebeling, M. Kunz, M. Staab, C. Guimarães-Steinicke, D. Bachmann, N. Buchmann, W. Durka, A. Fichtner, F. Fornoff, W. Härdtle, L. R. Hertzog, A-N. Klein, C. Roscher, J. Schaller, von G. Oheimb, A. Weigelt, W. Weisser, C.Wirth, J. Zhang, H. Bruelheide, and N. Eisenhauer. 2019. Multiple plant diversity components drive consumer communities across ecosystems. Nature Communications 10:1460.

Tedersoo, L., M. Bahram, S. Põlme, U. Kõljalg, N. S. Yorou, R. Wijesundera, L. Villarreal Ruiz, A. M. Vasco-Palacios, P. Q. Thu, A. Suija, M. E. Smith, C. Sharp, E. Saluveer, A. Saitta, M. Rosas, T. Riit, D. Ratkowsky, K. Pritsch, K. Põldmaa, M. Piepenbring, C. Phosri, M. Peterson, K. Parts, K. Pärtel, E. Otsing, E. Nouhra, A. L. Njouonkou, R. H. Nilsson, L. N. Morgado, J. Mayor, T. W. May, L. Majuakim, D. J. Lodge, S. See Lee, K.-H. Larsson, P. Kohout, K. Hosaka, I. Hiiesalu, T. W. Henkel, H. Harend, L.-D. Guo, A. Greslebin, G. Grelet, J. Geml, G. Gates, W. Dunstan, C. Dunk, R. Frenkhan, L. Dearnaley, A. De Kesel, T. Dang, X. Chen, F. Buegger, F. Q. Brearley, G. Bonito, S. Anslan, S. Abell, and K. Abarenkov. 2014. Global diversity and geography of soil fungi. Science 346:1256688.

Thomas, G.W. 1982. Exchangeable cations. Pages 159-165 in A. L. Page, R. H. Miller, and D. R. Keeney, editors. Methods of Soil Analysis: Part 2—Chemical and microbiological properties. Soil Science Society of America (SSSA) Inc., Madison.

Walthert, L., U. Graf, A. Kammer, J. Luster, D. Pezzotta, S. Zimmermann, and F. Hagedorn. 2010. Determination of organic and inorganic carbon, δ13C, and nitrogen in soils containing carbonates after acid fumigation with HCl. Journal of Plant Nutrition and Soil Sciences 173:207-216.

Yeates, G. W., T. Bongers, R. G. M. de Goede, D. W. Freckman, and S. S. Georgieva. 1993. Feeding habits in soil nematode families and genera – an outline for soil ecologists. Journal of Nematology 25:315-331.

bacteria

display_name: bacteria

biodiversity

display_name: biodiversity

ecosystem-multifunctionality

display_name: ecosystem-multifunctionality

fungi

display_name: fungi

grassland

display_name: grassland

mineralization

display_name: mineralization

nematodes

display_name: nematodes

plants

display_name: plants

restoration

display_name: restoration

soil-biodiversity

display_name: soil-biodiversity

educ

en: Education, culture and sport

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Restoring grassland multifunctionality


maintainer: Roman Zweifel

en: eddy-covariance forest-function open-data forest subalpine forest-canopy forest-characteristics ecosystem-functioning ilter forest-ecosystems sap-flow carbon-cycle lwf forest-biomass ecophysiology water-relations picea-abies stem-growth davos dendrometer forest-area dbh carbon-sequestration icos tree-stem carbon-storage carbon-dioxide twd climate-growth flux metadata automated-monitoring co2 eddy-covariance-measurements forest-monitoring biomass treenet atmospheric-conditions seehornwald climate-change icp-forests forest-dynamics

en: Collection of files with information about the research site Seehornwald Davos CH-DAV

en: Readme

en: Site description of the Seehornwald Davos (CH-DAV) research site. The material provided offers information on the biomass and growth of trees in CH-DAV, including supporting materials such as maps, geolocations and analyses. CH-DAV is part of networks such as ICOS, LWF, TreeNet, ICPForests and is dedicated to forest monitoring and research.

atmospheric-conditions

display_name: atmospheric-conditions

automated-monitoring

display_name: automated-monitoring

biomass

display_name: biomass

carbon-cycle

display_name: carbon-cycle

carbon-dioxide

display_name: carbon-dioxide

carbon-sequestration

display_name: carbon-sequestration

carbon-storage

display_name: carbon-storage

climate-change

display_name: climate-change

climate-growth

display_name: climate-growth

davos

display_name: davos

dbh

display_name: dbh

dendrometer

display_name: dendrometer

ecophysiology

display_name: ecophysiology

ecosystem-functioning

display_name: ecosystem-functioning

eddy-covariance

display_name: eddy-covariance

eddy-covariance-measurements

display_name: eddy-covariance-measurements

flux

display_name: flux

forest

display_name: forest

forest-area

display_name: forest-area

forest-biomass

display_name: forest-biomass

forest-canopy

display_name: forest-canopy

forest-characteristics

display_name: forest-characteristics

forest-dynamics

display_name: forest-dynamics

forest-ecosystems

display_name: forest-ecosystems

forest-function

display_name: forest-function

forest-monitoring

display_name: forest-monitoring

icos

display_name: icos

icp-forests

display_name: icp-forests

ilter

display_name: ilter

lwf

display_name: lwf

metadata

display_name: metadata

open-data

display_name: open-data

picea-abies

display_name: picea-abies

sap-flow

display_name: sap-flow

seehornwald

display_name: seehornwald

stem-growth

display_name: stem-growth

subalpine

display_name: subalpine

tree-stem

display_name: tree-stem

treenet

display_name: treenet

twd

display_name: twd

water-relations

display_name: water-relations

en: Site description Davos Seehornwald CH-DAV


maintainer: Jana von Freyberg

en: plant-water-uptake ecohydrology stable-water-isotopes uncertainties root-zone endmember-mixing-models review

en: Data set of MEE-20-04-264

en: The following two tables contain information about the data sources of the values reported in Table 1 and 2 in the paper “Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources” published in the journal ‘Methods in Ecology and Evolution’.

en: The following two tables contain information about the data sources of the values reported in Table 1 and 2 in the paper “Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources” published in the journal 'Methods in Ecology and Evolution'.

ecohydrology

display_name: ecohydrology

endmember-mixing-models

display_name: endmember-mixing-models

plant-water-uptake

display_name: plant-water-uptake

review

display_name: review

root-zone

display_name: root-zone

stable-water-isotopes

display_name: stable-water-isotopes

uncertainties

display_name: uncertainties

en: Data set of: Plant and root-zone water isotopes are difficult to measure, explain, and predict: some practical recommendations for determining plant water sources


maintainer: Felix Gugerli

en: monitoring melanargia-galathea genetic-diversity conservation-genetics genetic-structure

en:
Individual genotypes assessed at six nuclear microsatellite loci (two alleles per locus) for individuals of the butterfly Melanargia galathea (Marbled white) that were collected throughout Switzerland, along the regular grid of the Biodiversity Monitoring (BDM) of Switzerland. For each individual, the sampling site (number) and the genotype are given. Note that due to privacy restrictions, the original geographic coordinates remain disclosed. For original coordinates, data holders should be contacted (Swiss Federal Office for the Environment; Biodiversity Monitoring Switzerland).
The data refer to the following publication: Terzer et al., Distinct spatial patterns of genetic structure and diversity in the butterfly Marbled White (Melanargia galathea) inhabiting fragmented grasslands. Conservation Genetics, https://doi.org/0.1007/s10592-023-01593-4.

conservation-genetics

display_name: conservation-genetics

genetic-diversity

display_name: genetic-diversity

genetic-structure

display_name: genetic-structure

melanargia-galathea

display_name: melanargia-galathea

monitoring

display_name: monitoring

en: Nuclear microsatellite genotypes of the butterfly Melanargia galathea


maintainer: Virginie Marques

en: fish-composition marseille marine teleo edna mediterraneaen

en: Data access

en: Please use cyberduck to download for the moment

en:
Description: Fish environmental DNA data set collected in 2022 in the Calanques National Park
The eDNA samples were collected in 2022 in two locations (Moyades, M−FPA and “Impérial du large”, I-LPA), during the winter (January- February), the summer (June to August)and fall (mid-September to November) seasons, with the sampling dates depending on the weather conditions. For the M−FPA, samples were collected between Moyades island and Riou island, while for the I-LPA, they were collected on the south side of the “Impérial du large” island. To account for the existing bathymetry, in the M−FPA the samples were taken at two sampling sites with depths of 20 and 40 m, while in the I-LPA they were taken at two sites with depths of 20 and 80 m.
At each site, in-situ filtration of seawater was performed using a double-head submersible pump (Subspace, Geneva, Switzerland; nominal flow of ca. 1 L/min) strapped to an underwater scooter with 2 VigiDNA 0.20 µm filtration capsules (SPYGEN, le Bourget du Lac, France), along with disposable sterile tubing. The samples were collected along two horizontal transects (up to 400 m in length) during each closed-circuit rebreather dive, enabling the filtration of a water volume of 15 L/filter per depth, as close as possible to the substrate. Two filter replicates were collected by two divers at each sampling site, except in two cases where bad weather conditions or logistical issues meant that only one replicate was sampled.
After the filtration, the remaining seawater was emptied from the capsule back on the boat and replaced by a 80 mL CL1 conservation buffer (SPYGEN, le Bourget du Lac, France). To prevent any contamination, a strict protocol was followed during the entire process, requiring disposable gloves and single-use filtration equipment. Finally, the samples were stored at room temperature. We followed a strict contamination control protocol in both field and laboratory stages. Each water sample processing included the use of disposable gloves and single-use filtration equipment to avoid any risk of contamination. Libraries were prepared with ligation using the MetaFast protocol (Fasteris).
Data content:
	* rawdata/: contains the raw reads for each individual sample. One archive contains the paired-end reads specified by the _R1 or _R2 suffix as well as individually tagged PCR replicates (if available) together with an archive containing all extraction and PCR blank samples of the library. Reads have been demultiplexed using cutadapt but not trimmed, individual demultiplexing tags and primers remain present in the sequences.
	* taxadata/: contains the table with all detected taxonomy for each sample after bioinformatic processing (see Polanco et al. 2020 for details; https://doi.org/10.1002/edn3.140) and associated field metadata.
	* metadata/: contains two metadata files, one related to the data collected in the field for each filter, and the second related to the sequencing process in the lab (including the tag sequence, library name, and marker information for each sample)

edna

display_name: edna

fish-composition

display_name: fish-composition

marine

display_name: marine

marseille

display_name: marseille

mediterraneaen

display_name: mediterraneaen

teleo

display_name: teleo

educ

en: Education, culture and sport

en: Environmental DNA Marine France Calanques 2022


en: trap-nest urbanization wasps food-webs bees management-intensity hymenoptera cavity-nesting urban-ecology host-enemy-interactions zurich urban-ecosystems

en: Questionnaires management intensity anonymised

en: Results from the management intensity questionnaires anonymised. IDs are randomized and don't match those in the study data csv

en: Script for the analyses v1.0

en: Cleaned script to reproduce the analyses

format: OCTET

en: Read me file

en: Raw data nests

en: Host-enemy interaction data, used to compute the interaction diversity metrics

en: Data of the study

en: The csv. file contains the response variable "interaction diversity" (idiv), and the values of the predictors used in the paper. The "id" column indicates the garden ID, each row in the dataset correspond to a garden and each column to the response (idiv), an explanatory variable or a descriptor (name, Lat, Long). See the thesaurus and the readme file for extended information on the variables.

en: Thesaurus

en: Variable names and their description.

en: Not used: data of the study

en: Script for the analyses v2.0

en: Not used: raw questionnaires

en: Not used: readme

en:
The data supports the paper "Local and landscape factors shape alpha and beta trophic interaction diversity in urban gardens". It provides:
(1) the host-natural enemy interaction networks and network metrics between cavity-nesting bees and wasps and their natural enemies,
 (2) the explanatory variables reflecting urban intensity, temperature, habitat amount and management intensity.
(3) the questionnaires for assessing management intensity.
(4) a thesaurus for the variable names.
(5) a readme file.
(6) the R script used for the analyses.
The data is part of the BetterGardens project. It was sampled in 85 allotment and home gardens in the ctiy of Zurich in 2015 using standardized trapnests.

bees

display_name: bees

cavity-nesting

display_name: cavity-nesting

food-webs

display_name: food-webs

host-enemy-interactions

display_name: host-enemy-interactions

hymenoptera

display_name: hymenoptera

management-intensity

display_name: management-intensity

trap-nest

display_name: trap-nest

urban-ecology

display_name: urban-ecology

urban-ecosystems

display_name: urban-ecosystems

urbanization

display_name: urbanization

wasps

display_name: wasps

zurich

display_name: zurich

envidat

en: EnviDat: the Environmental Data Portal

en: EnviDat is the WSL data portal providing unified and managed access to environmental monitoring and research data. The portal has the capability to host and publish data sets. Through the sharing of unique and high-value data sets worldwide, EnviDat fosters research and contributes to advancing environmental science.

en: Data on interaction diversity in urban gardens


en: erosion snow mars model sediment-transport

en: Datasets of Figure 2

en: Datasets of Figure 4b

en: Datasets of Figure 3a

en: Datasets of Figure 3b

en: Datasets of Figure 4a

en:
The files contain the datasets used to produce Figures 2, 3, and 4 of the manuscript ([doi: 10.1002/2016GL071822](http://dx.doi.org/10.1002/2016GL071822)).

 Manuscript Abstract:
Despite being the main sediment entrainment mechanism in aeolian transport, granular splash is still poorly understood. We provide a deeper insight into the dynamics of sand and snow ejection with a stochastic model derived from the energy and momentum conservation laws. Our analysis highlights that the ejection regime of uniform sand is inherently different from that of heterogeneous sand. Moreover, we show that cohesive snow presents a mixed ejection regime, statistically controlled either by energy or momentum conservation depending on the impact velocity. The proposed formulation can provide a solid base for granular splash simulations in saltation models, leading to more reliable assessments of aeolian transport on Earth and Mars.

erosion

display_name: erosion

mars

display_name: mars

model

display_name: model

sediment-transport

display_name: sediment-transport

snow

display_name: snow

educ

en: Education, culture and sport

en: Energy- and momentum-conserving model of splash entrainment in sand and snow saltation


maintainer: Alexandre Buttler

en: transplantation-experiment biogeochemisty soil silvopastoral-systems climate-change

en: Dataset

en: Zip archive of all datasets detailed in study.

mimetype: application/zip

en:
Silvopastoral systems are highly productive and combine long-term wood production with annual provision of forage for livestock. In the Swiss Jura Mountains these systems are a key component of the landscape. As in other cold biomes, climate change can potentially accelerate landscape change within these historically sustainable systems.

In order to anticipate the evolution of subalpine wooded pasture ecosystems under future climate and land-use changes, this project focused on the interplay between soil, vegetation and climate. It was aimed at providing experimental evidence for chief ecosystem processes, with emphasis on the quality of the ecosystem services provided. The main interest was placed on vegetation turf resistance to climate change along an unwooded – sparsely wooded - densely wooded pasture gradient (land-use intensity), where plant productivity, diversity and succession along with rates of carbon cycling and microbial activity provided measures of ecosystem functioning at both plot and landscape level.

Experimental transplantation of monolith soil turfs to lower altitudes allowed to simulate soil warming and reduced annual precipitation. In order to simulate a year-round warmer and drier climate the natural climate variation along an altitudinal gradient was used as a proxy. The aim was to simulate realistic climate change scenarios for the second half of the 21st century predicted by the IPCC report and downscaled for Switzerland providing regionalized interpolated projections integrating therein trends for temperature increase and precipitation decrease. By using permanent meteorological stations within the network of the Federal Office of Meteorology and Climatology (MeteoSwiss), we obtained high resolution regional data on the variation of mean annual temperature (MAT) and mean annual precipitation (MAP) in relation to altitude in the Swiss Jura Mountains. We observed a general increase of +0.5 K in MAT and a decrease of -20 % MAP for each 100 m decrease in altitude along the SE slope of the Swiss Jura Mountains. These relationships served for the selection of the transplantation sites such that in comparison to a control site at 1350 m a.s.l. (Combe des Amburnex, N 46°54’, E 6°23’) a +2 K MAT and -20 % MAP was achieved at 1010 m a.s.l. (Saint-George, N 46°52’, E 6°26’), a +4 K MAT and -40 % MAP at 570 m a.s.l., (Arboretum d’Aubonne, N 46°51’, E 6°37’), and a +5 K MAT and -50 % MAP at 395 m a.s.l. (Les Bois Chamblard, N 46°47’, E 6°41’). The two stations at 1010 m a.s.l. and 570 m a.s.l. corresponded to the IPCC scenario A1B for a moderate increase in greenhouse gas emissions and to scenario A2 for a high increase in greenhouse gas emissions, respectively. The station at 395 m a.s.l. was chosen to represent an extreme scenario with climate variables lying at the positive tail distribution of model predictions under the A2 scenario.

Soil cores were assembled into rectangular PVC boxes of 60  80 cm2 size and of 35 cm height. All mesocosms were dug down to surface level into previously prepared trenches in the ground thus preventing lateral heat exchange with the atmosphere. Since at each site the mesocosms were placed in a common garden with no light interception, mesocosms with turfs from the two wooded pastures were shaded from direct sun light to simulate the natural light conditions in the corresponding habitats. Each mesocosm was equipped with a drainage system and was connected to a water tank thus representing a zero potential lysimeter collecting soil solution and precipitation/snowmelt runoff. ECH2O EC-TM sensor probes coupled to Em50 data-loggers (Decagon Devices, Inc., USA) recorded soil temperature and volumetric water content in each mesocosm at the top-soil (0 to -3 cm) every minute and data were averaged over one hour intervals. Climate parameters at each transplantation site were monitored continuously throughout the experiment by means of automated weather stations (Sensor Scope Sàrl, Switzerland), measuring rain precipitation (non-heated tipping bucket gauges) and air temperature and humidity 2 m above the ground surface at one minute intervals.

A list of above- and belowground variables were measured to assess the resilience of biogeochemical processes, plant productivity, tree regeneration, and carbon sequestration for each respective land-use practice. Furthermore, the experimental data were used to improve on (parameterization) the existing spatially explicit, dynamic model WoodPaM and refine the modelʼs climatic and land-use variables so that different scenarios of climate change and land use change could be simulated. Natural and management induced disturbance patterns were incorporated into the model.
The data have been made available within the project CCES Mounted. The climate stations Sensorscope are still in use within the project CLIMARBRE (Wald und Klimawandel, WSL/BAFU).

#References

1. Puissant, J., Cécillon, L., Mills, R.T.E., Robroek, B.J.M. Gavazov, K., De Danieli, S., Spiegelberger, T., Buttler, A., Brun, J.J. 2015. Seasonal influence of climate manipulation on microbial community structure and function in mountain soils. Soil Biology and Biochemistry 80: 296–305.
2. Mills, R., K. Gavazov, T. Spiegelberger, D. Johnson and A. Buttler 2014. Diminished soil functions occur under simulated climate change in a sup-alpine pasture, but heterotrophic temperature sensitivity indicates microbial resilience. Science of the Total Environment, vol. 473–474(0): 465-472.
3. Gavazov, K., Spiegelberger, T. and Buttler, A. 2014. Transplantation of subalpine wood-pasture turfs along a natural climatic gradient reveals lower resistance of unwooded pastures to climate change compared to wooded ones. Oecologia (174) : 1425-1435.
4. Peringer A., Siehoff S., Chételat J., Spiegelberger T., Buttler A. & Gillet F. 2013. Past and future landscape dynamics in pasture-woodlands of the Swiss Jura Mountains under climate change. Ecology and Society, 18, 3: 11. DOI: 10.5751/ES-05600-180311. [online] URL: http://www.ecologyandsociety.org/vol18/iss3/art11/
5. Gavazov, K. S., A. Peringer, A. Buttler, F. Gillet and T. Spiegelberger. 2013. Dynamics of Forage Production in Pasture-woodlands of the Swiss Jura Mountains under Projected Climate Change Scenarios. Ecology and Society 18 (1): 38. [online] URL: http://www.ecologyandsociety.org/vol18/iss1/art38/

biogeochemisty

display_name: biogeochemisty

climate-change

display_name: climate-change

silvopastoral-systems

display_name: silvopastoral-systems

soil

display_name: soil

transplantation-experiment

display_name: transplantation-experiment

en: Biogeochemical data from a transplantation experiment of monolith soil turfs along an altitudinal gradient to simulate climate change scenarios